Abscisic acid-mediated phytochrome B signaling promotes primary root growth in Arabidopsis

Abscisic acid (ABA) is a crucial factor that affects primary root tip growth in plants. Previous research suggests that reactive oxygen species (ROS), especially hydrogen peroxide, are important regulators of ABA signalling in root growth of Arabidopsis. PROLINE-RICH EXTENSIN-LIKE RECEPTOR KINASE 4 (PERK4) plays an important role in ABA responses. Arabidopsis perk4 mutants display attenuated sensitivity to ABA, especially in primary root growth. To gain insights into the mechanism(s) of PERK4-associated ABA inhibition of root growth, in this study we investigated the involvement of ROS in this process. Normal ROS accumulation in the primary root in response to exogenous ABA treatment was not observed in perk4 mutants. PERK4 deficiency prohibits ABA-induced expression of RESPIRATORY BURST OXIDASE HOMOLOGUE (RBOH) genes, therefore the perk4-1 mutant showed decreased production of ROS in the root. The perk4-1/rbohc double mutant displayed the same phenotype as the perk4 and rbohc single mutants in response to exogenous ABA treatment. The results suggest that PERK4-stimulated ROS accumulation during ABA-regulated primary root growth may be mediated by RBOHC.

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Mechanisms for Abscisic Acid Inhibition of Primary Root Growth

Plant Signaling & Behavior ◽

10.1080/15592324.2018.1500069 ◽

2018 ◽

Vol 13 (9) ◽

pp. e1500069 ◽

Cited By ~ 16

Author(s):

Li Rong Sun ◽

Yi Bin Wang ◽

Shi Bin He ◽

Fu Shun Hao

Keyword(s):

Abscisic Acid ◽

Root Growth ◽

Primary Root ◽

Acid Inhibition ◽

Primary Root Growth

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The Nitrate Transporter MtNPF6.8 (MtNRT1.3) Transports Abscisic Acid and Mediates Nitrate Regulation of Primary Root Growth in Medicago truncatula

PLANT PHYSIOLOGY ◽

10.1104/pp.114.250811 ◽

2014 ◽

Vol 166 (4) ◽

pp. 2152-2165 ◽

Cited By ~ 27

Author(s):

Anthoni Pellizzaro ◽

Thibault Clochard ◽

Caroline Cukier ◽

Céline Bourdin ◽

Marjorie Juchaux ◽

...

Keyword(s):

Abscisic Acid ◽

Root Growth ◽

Medicago Truncatula ◽

Primary Root ◽

Nitrate Transporter ◽

Primary Root Growth

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Increased Endogenous Abscisic Acid Maintains Primary Root Growth and Inhibits Shoot Growth of Maize Seedlings at Low Water Potentials

PLANT PHYSIOLOGY ◽

10.1104/pp.93.4.1329 ◽

1990 ◽

Vol 93 (4) ◽

pp. 1329-1336 ◽

Cited By ~ 208

Author(s):

Imad N. Saab ◽

Robert E. Sharp ◽

Jeremy Pritchard ◽

Gary S. Voetberg

Keyword(s):

Abscisic Acid ◽

Root Growth ◽

Shoot Growth ◽

Primary Root ◽

Maize Seedlings ◽

Endogenous Abscisic Acid ◽

Water Potentials ◽

Primary Root Growth

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AtrbohD and AtrbohF positively regulate abscisic acid-inhibited primary root growth by affecting Ca2+ signalling and auxin response of roots in Arabidopsis

Journal of Experimental Botany ◽

10.1093/jxb/ert228 ◽

2013 ◽

Vol 64 (14) ◽

pp. 4183-4192 ◽

Cited By ~ 54

Author(s):

Yiheng Jiao ◽

Lirong Sun ◽

Yalin Song ◽

Limin Wang ◽

Liping Liu ◽

...

Keyword(s):

Abscisic Acid ◽

Root Growth ◽

Primary Root ◽

Auxin Response ◽

Primary Root Growth

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CRISPR/Cas9-targeted mutagenesis of OsERA1 confers enhanced responses to abscisic acid and drought stress and increased primary root growth under nonstressed conditions in rice

PLoS ONE ◽

10.1371/journal.pone.0243376 ◽

2020 ◽

Vol 15 (12) ◽

pp. e0243376

Author(s):

Takuya Ogata ◽

Takuma Ishizaki ◽

Miki Fujita ◽

Yasunari Fujita

Keyword(s):

Abscisic Acid ◽

Drought Stress ◽

Stress Response ◽

Root Growth ◽

Primary Root ◽

Targeted Mutagenesis ◽

Negative Regulator ◽

Aba Signaling ◽

Primary Root Growth ◽

Mutant Lines

Abscisic acid (ABA) signaling components play an important role in the drought stress response in plants. Arabidopsis thaliana ENHANCED RESPONSE TO ABA1 (ERA1) encodes the β-subunit of farnesyltransferase and regulates ABA signaling and the dehydration response. Therefore, ERA1 is an important candidate gene for enhancing drought tolerance in numerous crops. However, a rice (Oryza sativa) ERA1 homolog has not been characterized previously. Here, we show that rice osera1 mutant lines, harboring CRISPR/Cas9-induced frameshift mutations, exhibit similar leaf growth as control plants but increased primary root growth. The osera1 mutant lines also display increased sensitivity to ABA and an enhanced response to drought stress through stomatal regulation. These results illustrate that OsERA1 is a negative regulator of primary root growth under nonstressed conditions and also of responses to ABA and drought stress in rice. These findings improve our understanding of the role of ABA signaling in the drought stress response in rice and suggest a strategy to genetically improve rice.

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