Improvement of antioxidant activity of olive mill wastewater phenolic compounds by Lactobacillus plantarum fermentation

The production of olive oil generates massive quantities of by-product called olive mill wastewater (OMWW). The uncontrolled disposal of OMWW poses serious environmental problems. The OMWW effluent is rich in several polyphenolic compounds. Liquid-liquid extraction of OMWW using ethyl acetate solvent was used to enrich phenolic compounds under investigation. Total phenolic and flavonoid content and antioxidant activity of the extract were determined. HPLC coupled to photodiode array (PDA) detector was used to analyze the main three phenolic compounds of OMWW, namely, hydroxytyrosol, tyrosol, and oleuropein. The antimicrobial activity of the extract was also investigated. Additionally, the OMWW extract was used as natural preservative and antioxidants for olive oil. Results showed that OMWW is very rich in phenolic compounds and has strong antioxidant activity. HPLC analysis showed that the extract contains mainly hydroxytyrosol and tyrosol but no oleuropein. The OMWW extract showed also positive activities as antibacterial (gram positive and gram negative) and antifungal as well as activities against yeast. The addition of OMWW extract to olive oil samples has an effect on the stability of olive oil as reflected by its acid value, peroxide value, K232 and K270, and total phenolic content.

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Antioxidant Properties of Olive Mill Wastewater Polyphenolic Extracts on Human Endothelial and Vascular Smooth Muscle Cells

Foods ◽

10.3390/foods10040800 ◽

2021 ◽

Vol 10 (4) ◽

pp. 800

Author(s):

Anna Maria Posadino ◽

Annalisa Cossu ◽

Roberta Giordo ◽

Amalia Piscopo ◽

Wael M Abdel-Rahman ◽

...

Keyword(s):

Antioxidant Activity ◽

Cell Death ◽

Smooth Muscle ◽

Vascular Smooth Muscle ◽

Smooth Muscle Cells ◽

Olive Oil ◽

Vascular Smooth Muscle Cells ◽

Muscle Cells ◽

Olive Mill Wastewater ◽

Olive Mill

This work aims to analyze the chemical and biological evaluation of two extracts obtained by olive mill wastewater (OMW), an olive oil processing byproduct. The exploitation of OMW is becoming an important aspect of development of the sustainable olive oil industry. Here we chemically and biologically evaluated one liquid (L) and one solid (S) extract obtained by liquid–liquid extraction followed by acidic hydrolysis (LLAC). Chemical characterization of the two extracts indicated that S has higher phenol content than L. Hydroxytyrosol and tyrosol were the more abundant phenols in both OMW extracts, with hydroxytyrosol significantly higher in S as compared to L. Both extracts failed to induce cell death when challenged with endothelial cells and vascular smooth muscle cells in cell viability experiments. On the contrary, the higher extract dosages employed significantly affected cell metabolic activity, as indicated by the MTT tests. Their ability to counteract H2O2-induced oxidative stress and cell death was assessed to investigate potential antioxidant activities of the extracts. Fluorescence measurements obtained with the reactive oxygen species (ROS) probe H2DCF-DA indicated strong antioxidant activity of the two OMW extracts in both cell models, as indicated by the inhibition of H2O2-induced ROS generation and the counteraction of the oxidative-induced cell death. Our results indicate LLAC-obtained OMW extracts as a safe and useful source of valuable compounds harboring antioxidant activity.

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Synthesis, characterization and evaluation of antioxidant activity of tyrosol derivatives from olive mill wastewater

Chemical Papers ◽

10.1007/s11696-018-0591-7 ◽

2018 ◽

Vol 73 (3) ◽

pp. 663-671

Author(s):

Zeinab Namazifar ◽

Fariba Saadati ◽

Ali Akbar Miranbeigi

Keyword(s):

Antioxidant Activity ◽

Olive Mill Wastewater ◽

Olive Mill

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Continuous flow adsorption of phenolic compounds from olive mill wastewater with resin XAD16N: life cycle assessment, cost–benefit analysis and process optimization

Journal of Chemical Technology & Biotechnology ◽

10.1002/jctb.5980 ◽

2019 ◽

Vol 94 (6) ◽

pp. 1968-1981 ◽

Cited By ~ 4

Author(s):

Dario Frascari ◽

Aurora Esther Molina Bacca ◽

Tjerk Wardenaar ◽

Emmanuel Oertlé ◽

Davide Pinelli

Keyword(s):

Life Cycle Assessment ◽

Life Cycle ◽

Phenolic Compounds ◽

Process Optimization ◽

Continuous Flow ◽

Cost Benefit Analysis ◽

Cost Benefit ◽

Olive Mill Wastewater ◽

Benefit Analysis ◽

Olive Mill

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Amine-modified mesoporous silica for quantitative adsorption and release of hydroxytyrosol and other phenolic compounds from olive mill wastewater

Journal of the Taiwan Institute of Chemical Engineers ◽

10.1016/j.jtice.2016.10.053 ◽

2017 ◽

Vol 70 ◽

pp. 111-118 ◽

Cited By ~ 19

Author(s):

Asma Yangui ◽

Manef Abderrabba ◽

Abdelhamid Sayari

Keyword(s):

Phenolic Compounds ◽

Mesoporous Silica ◽

Olive Mill Wastewater ◽

Adsorption And Release ◽

Olive Mill

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Biodegradation of olive mill wastewater phenolic compounds in a thermophilic anaerobic upflow packed bed reactor and assessment of their toxicity in digester effluents

Journal of Environmental Management ◽

10.1016/j.jenvman.2019.109882 ◽

2020 ◽

Vol 255 ◽

pp. 109882 ◽

Cited By ~ 5

Author(s):

C. Genethliou ◽

M. Kornaros ◽

S. Dailianis

Keyword(s):

Phenolic Compounds ◽

Packed Bed ◽

Olive Mill Wastewater ◽

Packed Bed Reactor ◽

Olive Mill

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Biodegradation of Olive Mill Wastewater by Trichosporon Cutaneum and Geotrichum Candidum

Archives of Industrial Hygiene and Toxicology ◽

10.2478/10004-1254-61-2010-2079 ◽

2010 ◽

Vol 61 (4) ◽

pp. 399-405 ◽

Cited By ~ 4

Author(s):

Tibela Dragičević ◽

Marijana Hren ◽

Margareta Gmajnić ◽

Sanja Pelko ◽

Dzoko Kungulovski ◽

...

Keyword(s):

Phenolic Compounds ◽

Oxygen Demand ◽

Geotrichum Candidum ◽

Olive Mill Wastewater ◽

Trichosporon Cutaneum ◽

Aerobic Conditions ◽

Wide Range ◽

Dark Colour ◽

Static Conditions ◽

Olive Mill

Biodegradation of Olive Mill Wastewater by Trichosporon Cutaneum and Geotrichum CandidumOlive oil production generates large volumes of wastewater. These wastewaters are characterised by high chemical oxygen demand (COD), high content of microbial growth-inhibiting compounds such as phenolic compounds and tannins, and dark colour. The aim of this study was to investigate biodegradation of olive mill wastewater (OMW) by yeasts Trichosporon cutaneum and Geotrichum candidum. The yeast Trichosporon cutaneum was used because it has a high potential to biodegrade phenolic compounds and a wide range of toxic compounds. The yeast Geotrichum candidum was used to see how successful it is in biodegrading compounds that give the dark colour to the wastewater. Under aerobic conditions, Trichosporon cutaneum removed 88 % of COD and 64 % of phenolic compounds, while the dark colour remained. Geotrichum candidum grown in static conditions reduced COD and colour further by 77 % and 47 %, respectively. This investigation has shown that Trichosporon cutaneum under aerobic conditions and Geotrichum candidum under facultative anaerobic conditions could be used successfully in a two-step biodegradation process. Further investigation of OMW treatment by selected yeasts should contribute to better understanding of biodegradation and decolourisation and should include ecotoxicological evaluation of the treated OMW.

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