Clerodendrum trichotomum extract improves metabolic derangements in high fructose diet-fed rats

Abstract Diet is believed to be an important factor in the pathogenesis of Inflammatory Bowel Disease. High consumption of dietary fructose has been shown to exacerbate experimental colitis, an effect mediated through the gut microbiota. This study evaluated whether dietary alterations could attenuate the detrimental effects of a high fructose diet (HFrD) in experimental colitis. First, we determined whether the pro-colitic effects of a HFrD could be reversed by switching mice from a HFrD to a control diet. This diet change completely prevented HFrD-induced worsening of acute colitis, in association with a rapid normalization of the microbiota. Second, we tested the effects of dietary fiber, which demonstrated that psyllium was the most effective type of fiber for protecting against HFrD-induced worsening of acute colitis, compared to pectin, inulin or cellulose. In fact, supplemental psyllium nearly completely prevented the detrimental effects of the HFrD, an effect associated with a shift in the gut microbiota. We next determined whether the protective effects of these interventions could be extended to chronic colitis and colitis-associated tumorigenesis. Using the azoxymethane/dextran sodium sulfate model, we first demonstrated that HFrD feeding exacerbated chronic colitis and increased colitis-associated tumorigenesis. Using the same dietary changes tested in the acute colitis setting, we also showed that mice were protected from HFrD-mediated enhanced chronic colitis and tumorigenesis, upon either diet switching or psyllium supplementation. Taken together, these findings suggest that high consumption of fructose may enhance colon tumorigenesis associated with long-standing colitis, an effect that could be reduced by dietary alterations.

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Dietary Supplementation with Exogenous Sea-Cucumber-Derived Ceramides and Glucosylceramides Alleviates Insulin Resistance in High-Fructose-Diet-Fed Rats by Upregulating the IRS/PI3K/Akt Signaling Pathway

Journal of Agricultural and Food Chemistry ◽

10.1021/acs.jafc.0c06831 ◽

2021 ◽

Author(s):

Jin-Yue Yang ◽

Tian-Tian Zhang ◽

Zhe Dong ◽

Hao-Hao Shi ◽

Jie Xu ◽

...

Keyword(s):

Insulin Resistance ◽

Signaling Pathway ◽

Sea Cucumber ◽

Dietary Supplementation ◽

Akt Signaling ◽

Akt Signaling Pathway ◽

High Fructose Diet ◽

High Fructose

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High-Fructose Diet Increases Inflammatory Cytokines and Alters Gut Microbiota Composition in Rats

Mediators of Inflammation ◽

10.1155/2020/6672636 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10 ◽

Cited By ~ 1

Author(s):

Yong Wang ◽

Wentao Qi ◽

Ge Song ◽

Shaojie Pang ◽

Zhenzhen Peng ◽

...

Keyword(s):

Uric Acid ◽

Inflammatory Response ◽

Gut Microbiota ◽

Inflammatory Cytokines ◽

Proinflammatory Cytokines ◽

Lipid Accumulation ◽

Fasting Blood Glucose ◽

High Fructose Diet ◽

Fructose Intake ◽

High Fructose

High-fructose diet induced changes in gut microbiota structure and function, which have been linked to inflammatory response. However, the effect of small or appropriate doses of fructose on gut microbiota and inflammatory cytokines is not fully understood. Hence, the abundance changes of gut microbiota in fructose-treated Sprague-Dawley rats were analyzed by 16S rRNA sequencing. The effects of fructose diet on metabolic disorders were evaluated by blood biochemical parameter test, histological analysis, short-chain fatty acid (SCFA) analysis, ELISA analysis, and Western blot. Rats were intragastrically administered with pure fructose at the dose of 0 (Con), 2.6 (Fru-L), 5.3 (Fru-M), and 10.5 g/kg/day (Fru-H) for 20 weeks. The results showed that there were 36.5% increase of uric acid level in the Fru-H group when compared with the Con group. The serum proinflammatory cytokines (IL-6, TNF-α, and MIP-2) were significantly increased ( P < 0.05 ), and the anti-inflammatory cytokine IL-10 was significantly decreased ( P < 0.05 ) with fructose treatment. A higher fructose intake induced lipid accumulation in the liver and inflammatory cell infiltration in the pancreas and colon and increased the abundances of Lachnospira, Parasutterella, Marvinbryantia, and Blantia in colonic contents. Fructose intake increased the expressions of lipid accumulation proteins including perilipin-1, ADRP, and Tip-47 in the colon. Moreover, the higher level intake of fructose impaired intestinal barrier function due to the decrease of the expression of tight junction proteins (ZO-1 and occludin). In summary, there were no negative effects on body weight, fasting blood glucose, gut microbiota, and SCFAs in colonic contents of rats when fructose intake is in small or appropriate doses. High intake of fructose can increase uric acid, proinflammatory cytokines, intestinal permeability, and lipid accumulation in the liver and induce inflammatory response in the pancreas and colon.

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Effect of pomegranate extracts on brain antioxidant markers and cholinesterase activity in high fat-high fructose diet induced obesity in rat model

BMC Complementary and Alternative Medicine ◽

10.1186/s12906-017-1842-9 ◽

2017 ◽

Vol 17 (1) ◽

Cited By ~ 20

Author(s):

Zahra Amri ◽

Asma Ghorbel ◽

Mouna Turki ◽

Férièle Messadi Akrout ◽

Fatma Ayadi ◽

...

Keyword(s):

Rat Model ◽

Cholinesterase Activity ◽

High Fat ◽

High Fructose Diet ◽

Diet Induced Obesity ◽

High Fructose ◽

Antioxidant Markers

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Influence of Normo- and Hypogonadal Condition, Hyperuricemia, and High-Fructose Diet on Renal Changes in Male Rats

International Journal of Endocrinology ◽

10.1155/2017/1623597 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Jimena Soutelo ◽

Yanina Alejandra Samaniego ◽

Elsa Zotta ◽

María Cecilia Fornari ◽

Carlos Reyes Toso ◽

...

Keyword(s):

Gender Disparity ◽

Male Rats ◽

Renal Lesion ◽

Treatment Control ◽

High Fructose Diet ◽

High Fructose ◽

Renal Changes ◽

Male Wistar Rats ◽

Progression Of Renal Disease ◽

Oxonic Acid

Background. There is a gender disparity in the incidence, prevalence, and progression of renal disease. The object of this paper is to evaluate the presence and type of renal lesion in normogonadic and hypogonadic male rats in a mild hyperuricemia induced condition and exposed to a high-fructose diet. Methods. 56 adult male Wistar rats were used. Animals were divided into two groups, one normogonadic (NGN) and one hypogonadic (HGN), and each group was divided into four subgroups in accordance with the treatment: control with only water (C), fructose (F), oxonic acid (OA), and fructose + oxonic acid (FOA). Renal changes were evaluated by measuring glomerulosclerosis, fibrosis, and arteriolar media/lumen (M/L) ratio.Results. The OA and FOA groups presented significantly hypertension (p<0.001). The OA group significantly increased (p<0.05) the percentage of glomerulosclerosis as well as the FOA group (p<0.001). When comparing NGN versus HGN, we observed a trend to a lower glomerulosclerosis in the latter. A higher arteriolar M/L ratio was observed in the OA (p<0.05) and FOA (p<0.001). Conclusion. Hyperuricemia conditions and a high-fructose diet favor blood pressure increase together with changes in the arteriolar media/lumen ratio and renal glomerular damage. These changes were more apparent in normogonadic animals.

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