scholarly journals Wheel running predicts resilience to tumors in old mice

2019 ◽  
Vol 9 (1) ◽  
pp. 1676104
Author(s):  
Lida Zhu ◽  
Juan Wang ◽  
Christina Pettan-Brewer ◽  
Warren Ladiges ◽  
Jorming Goh
Keyword(s):  
Wheel Running ◽  
Old Mice

scholarly journals Exercise Increases Insulin Content and Basal Secretion in Pancreatic Islets in Type 1 Diabetic Mice

2011 ◽  
Vol 2011 ◽  
pp. 1-10 ◽  
Author(s):  
Han-Hung Huang ◽  
Kevin Farmer ◽  
Jill Windscheffel ◽  
Katie Yost ◽  
Mary Power ◽  
...  
Keyword(s):  
Insulin Secretion ◽  
Glycemic Control ◽  
Wheel Running ◽  
Insulin Content ◽  
Control Groups ◽  
Basal Secretion ◽  
Exercise Induced ◽  
Old Mice ◽  
Cell Proportion

Exercise appears to improve glycemic control for people with type 1 diabetes (T1D). However, the mechanism responsible for this improvement is unknown. We hypothesized that exercise has a direct effect on the insulin-producing islets. Eight-week-old mice were divided into four groups: sedentary diabetic, exercised diabetic, sedentary control, and exercised control. The exercised groups participated in voluntary wheel running for 6 weeks. When compared to the control groups, the islet density, islet diameter, andβ-cell proportion per islet were significantly lower in both sedentary and exercised diabetic groups and these alterations were not improved with exercise. The total insulin content and insulin secretion were significantly lower in sedentary diabetics compared to controls. Exercise significantly improved insulin content and insulin secretion in islets in basal conditions. Thus, some improvements in exercise-induced glycemic control in T1D mice may be due to enhancement of insulin content and secretion in islets.

scholarly journals Voluntary wheel running abolishes vascular inflammation and restores endothelial function in old mice

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Melanie L Connell ◽  
Jessica R Durrant ◽  
Molly J Russell ◽  
Anthony J Donato ◽  
Douglas R Seals ◽  
...  
Keyword(s):  
Endothelial Function ◽  
Wheel Running ◽  
Vascular Inflammation ◽  
Voluntary Wheel Running ◽  
Old Mice ◽  
Voluntary Wheel

scholarly journals CIRCADIAN PATTERNS OF FREE-WHEEL RUNNING IN YOUNG AND OLD MICE

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S911-S911
Author(s):  
Carly Hibbs ◽  
Musharraf Yusifova ◽  
Benjamin McNair ◽  
Danielle Bruns ◽  
Emily Schmitt
Keyword(s):  
Wheel Running ◽  
Delayed Response ◽  
Dark Phase ◽  
Peak Activity ◽  
Dark Cycle ◽  
Circadian Misalignment ◽  
Peripheral Muscle ◽  
The Impact ◽  
Old Mice ◽  
Young Mice

Abstract The mammalian circadian clock operates on a 24-hour cycle and regulates physiological, endocrine, and metabolic responses to changes in the environment. Aging disrupts this circadian process, increasing risk for development of age-associated diseases. Free-wheel running is not only an indicator of circadian rhythm, but also a strong predictor of survival from age-related diseases (i.e. cardiovascular disease). Thus, understanding the impact of age on free-wheel running can lead to a better understanding of disease progression. We analyzed free wheel running in both male and female C57BL/6J mice at young (3-6 months) and old (18-21 months) ages exposed to standard 12h light/dark cycle. Running wheel data was recorded hourly for 10 days. As expected, young female mice ran more than male mice, and old mice ran less than young mice. Regulation of wheel running demonstrated that older mice of both sexes had a delayed start time in activity patterns. Young mice began running immediately at lights off (signaling the start of their active period) and ran consistently throughout the dark phase with peak activity in the first 2 hours. In contrast, older mice had a delayed response to light with peak activity not occurring until hours 4-6 of the dark cycle and nightly activity ending 2 hours before lights on. Ongoing work will assess the central (brain) and peripheral (muscle, cardiac) regulation of free-wheel running in aging. Together, we demonstrate the importance of studying molecular mechanisms underlying circadian misalignment in older individuals to identify ways to combat age-associated disease with circadian misalignment.

Age-related myofiber atrophy in old mice is reversed by ten weeks voluntary high-resistance wheel running

2021 ◽  
Vol 143 ◽  
pp. 111150
Author(s):  
Annesofie Thorup Olesen ◽  
Lasse Malchow-Møller ◽  
Rune Duus Bendixen ◽  
Michael Kjær ◽  
René Brüggebusch Svensson ◽  
...  
Keyword(s):  
High Resistance ◽  
Wheel Running ◽  
Age Related ◽  
Old Mice

Abstract 222: Exercise Training Prevents Rarefaction of Pial Collaterals, Promotes Cerebral Arterial Remodeling, and Lessens Severity of Stroke in Aging Brain

Stroke ◽  
2017 ◽  
Vol 48 (suppl_1) ◽  
Author(s):  
Wojciech J Rzechorzek ◽  
Brian K Buckley ◽  
Kunji Hua ◽  
Daniel Pomp ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Exercise Training ◽  
Wheel Running ◽  
Important Determinant ◽  
Fiber Type ◽  
Infarct Volume ◽  
Cerebral Arteries ◽  
Aging Brain ◽  
Old Mice

Variation in anatomic extent of the collateral circulation is an important determinant of variation in the severity of ischemic stroke and efficacy of revascularization therapies. Yet pial collateral number and lumen diameter decrease with age, at least in mice. It is not known if this can be mitigated. We tested whether exercise training can accomplish this and if it also affects diameter of the posterior communicating collalterals (PComs) and primary cerebral arteries (ICA, BA, MCA, ACA, PCA). We randomized 30 male and 30 female, 12 months-old (~40 human years, hy) C57BL/6J mice to either sedentary or voluntary wheel-running (daily distance run was measured). At 25 mos-age (~70 hy), permanent MCA occlusion was followed 24h later by vascular casting after maximal dilation and by determination of infarct volume. Controls for aging were 3 mos-old sedentary mice (~16 hy). Training effect was confirmed by muscle fiber-type switching, body weight and cardiac hypertrophy (all p<0.05). Exercise prevented age-associated loss of collateral number and diameter (p=0.049 and 0.005, n=13-14) and reduced infarct volume by 50%, ie, to that seen in 3 months-old mice (p=0.01, n=7). Unlike pial collaterals, PCom diameter and number (ie, present bilaterally, unilaterally or absent) were unaffected by exercise. Of further interest, diameter of the primary cerebral arteries increased with aging alone (119 ± 1 vs 134 ± 5 μm averaged combined diameter, p=0.02, n=10-14); this effect tended to increase further with exercise (143 ± 4 μm, p=0.07, n=13). Mechanistically, exercise increased vascular expression (assessed by immunohisto-chemistry) of total eNOS (p=0.03, n=5-6), phospho-eNOS (p=0.004, n=5) and a marker of anti-oxidative stress (SOD, p=0.008, n=5-6; but not HO-1, p=0.40, n=6). It did not alter a marker of aging (p16, p=0.42, n=5); analysis of additional targets is underway. In conclusion, exercise training prevented age-induced rarefaction of pial collaterals and reduced infarct volume. In addition and unexpectedly, aging also caused outward remodeling of the primary cerebral arteries, and exercise training tended to further augment this. These benefits of regular aerobic exercise were associated with increased eNOS bioavailability and reduced oxidative stress.

Effects of aging on the circadian rhythm of wheel-running activity in C57BL/6 mice

1997 ◽  
Vol 273 (6) ◽  
pp. R1957-R1964 ◽  
Author(s):  
Verónica S. Valentinuzzi ◽  
Kathryn Scarbrough ◽  
Joseph S. Takahashi ◽  
Fred W. Turek
Keyword(s):  
Wheel Running ◽  
Activity Rhythm ◽  
Laboratory Mouse ◽  
Active Phase ◽  
Constant Darkness ◽  
Age Difference ◽  
Running Activity ◽  
Wheel Running Activity ◽  
Activity Onset ◽  
Old Mice

The effects of age on the circadian clock system have been extensively studied, mainly in two rodent species, the laboratory rat and the golden hamster. However, less information is available on how aging alters circadian rhythmicity in a commonly studied rodent animal model, the mouse. Therefore, in the present study we compared the rhythm of wheel-running activity in adult (6–9 mo) and old (19–22 mo) C57BL/6J mice maintained under different lighting conditions for a period of 4 mo. During this period, mice were subjected to phase advances and phase delays of the light-dark (LD) cycle and eventually to constant darkness (DD). In LD (12 h light, 12 h dark), old mice exhibited delayed activity onset relative to light offset and an increase in the variability of activity onset compared with adult mice. After a 4-h phase advance of the LD cycle, old mice took significantly longer to reentrain their activity rhythm when compared with adult animals. Old mice also demonstrated a decline in the number of wheel revolutions per day and a tendency toward a decrease in the length of the active phase. An increase in fragmentation of activity across the 24-h day was obvious in aging animals, with bouts of activity being shorter and longer rest periods intervening between them. No age difference was detected in the maximum intensity of wheel-running activity. In DD, the free-running period was significantly longer in old mice compared with adults. In view of the rapidly expanding importance of the laboratory mouse for molecular and genetic studies of the mammalian nervous system, the present results provide a basis at the phenotypic level to begin to apply genetic methods to the analysis of circadian rhythms and aging in mammals.

scholarly journals Aerobic exercise reverses arterial inflammation with aging in mice

2011 ◽  
Vol 301 (3) ◽  
pp. H1025-H1032 ◽  
Author(s):  
Lisa A. Lesniewski ◽  
Jessica R. Durrant ◽  
Melanie L. Connell ◽  
Grant D. Henson ◽  
Alexander D. Black ◽  
...  
Keyword(s):  
Aerobic Exercise ◽  
Vascular Function ◽  
Wheel Running ◽  
Vascular Dysfunction ◽  
Voluntary Wheel Running ◽  
Arterial Inflammation ◽  
Perivascular Fat ◽  
Aortic Intima ◽  
Old Mice ◽  
Voluntary Wheel

We tested the hypothesis that regular aerobic exercise reverses arterial inflammation with aging. When compared with young controls (6.2 ± 0.4 mo; n = 7), old (31.3 ± 0.5 mo; n = 11) male B6D2F1 cage-restricted mice demonstrated increased arterial activation of the proinflammatory transcription factor NF-κB, as indicated by greater aortic phosphorylation of both the inhibitor of NF-κB kinase (IKK) and the p65 subunit of NF-κB (both P < 0.05). Similarly, aortic expression of the proinflammatory cytokines IL-1 and IL-6, IFN-γ, and TNF-α were greater in the old mice (all P < 0.05). Macrophage and T lymphocyte abundance was unchanged with age in the aortic intima and media but was markedly increased in the adventitia and perivascular fat tissue of old mice (all P < 0.05). This proinflammatory arterial phenotype with aging was associated with vascular dysfunction, as reflected by impaired nitric oxide-mediated endothelium-dependent dilation. Voluntary wheel running (10–14 wk) normalized aortic IKK-NF-κB activation, cytokine expression, adventitial and perivascular macrophage infiltration, and vascular function in old mice (32.4 ± 0.3 mo; n = 8) while having no consistent effects in young mice. Short-term voluntary wheel running started late in life reverses arterial inflammation with aging in mice possibly via outside-in actions. These anti-inflammatory effects may play an important role in the amelioration of age-associated vascular dysfunction by regular aerobic exercise.

Incorporation of Cholesterol into Peripheral Nerve Myelin

1972 ◽  
Vol 30 ◽  
pp. 334-335
Author(s):  
Frank A. Rawlins
Keyword(s):  
Electron Microscope ◽  
Sciatic Nerve ◽  
Electron Microscope Autoradiography ◽  
Myelin Sheaths ◽  
Microscope Autoradiography ◽  
Grain Distribution ◽  
Sciatic Nerves ◽  
Autoradiographic Analysis ◽  
Old Mice ◽  
Short Times

Several speculations exist as to the site of incorporation of preformed molecules into myelin. The possibility that an autoradiographic analysis of cholesterol-1,2-H3 incorporation at very short times after injection might shed some light in the solution of that problem led to the present experiment.Cholesterol-1,2-H3 was injected intraperitoneally into 24 tenday old mice. The animals were then sacrificed at 10,20,30,40,60,90,120 and 180 min after the injection and the sciatic nerves were processed for electron microscope autoradiography. To analyze the grain distribution in the autoradiograms of cross and longitudinal sections from each sciatic nerve myelin sheaths were subdivided into three compartments named: outer 1/3, middle 1/3 and inner 1/3 compartments.It was found that twenty min. after the injection of cholesterol -1.2-H3 (Figs. 1 and 2), 55% of the total number of grains (t.n.g) found in myelin were within the outer 1/3 compartment, 9% were within the middle 1/3 and 36% within the inner 1/3 compartment

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