Multifunctional nanogel engineering with redox-responsive and AIEgen features for the targeted delivery of doxorubicin hydrochloride with enhanced antitumor efficiency and real-time intracellular imaging

Swarming micro/nanorobots offer great promise in performing targeted delivery inside diverse hard-to-reach environments. However, swarm navigation in dynamic environments challenges delivery capability and real-time swarm localization. Here, we report a strategy to navigate a nanoparticle microswarm in real time under ultrasound Doppler imaging guidance for active endovascular delivery. A magnetic microswarm was formed and navigated near the boundary of vessels, where the reduced drag of blood flow and strong interactions between nanoparticles enable upstream and downstream navigation in flowing blood (mean velocity up to 40.8 mm/s). The microswarm-induced three-dimensional blood flow enables Doppler imaging from multiple viewing configurations and real-time tracking in different environments (i.e., stagnant, flowing blood, and pulsatile flow). We also demonstrate the ultrasound Doppler–guided swarm formation and navigation in the porcine coronary artery ex vivo. Our strategy presents a promising connection between swarm control and real-time imaging of microrobotic swarms for localized delivery in dynamic environments.

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Redox Responsive Pluronic Micelle Mediated Delivery of Functional siRNA: A Modular Nano-Assembly for Targeted Delivery

Biomaterials Science ◽

10.1039/d1bm00428j ◽

2021 ◽

Author(s):

Sandeep Kadekar ◽

Ganesh N. Nawale ◽

Vignesh Kumar Rangasami ◽

Vadim Le Joncour ◽

Pirjo Laakkonen ◽

...

Keyword(s):

Targeted Delivery ◽

Unmet Need ◽

Short Interfering Rna ◽

Site Specific ◽

Redox Responsive ◽

Interfering Rna

There is an unmet need to develop strategies that allow site-specific delivery of short interfering RNA (siRNA) without any associated toxicity. To address this challenge, we have developed a novel...

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Anti-HER2 VHH Targeted Fluorescent Liposome as Bimodal Nanoparticle for Drug Delivery and Optical Imaging

Recent Patents on Anti-Cancer Drug Discovery ◽

10.2174/1574892816666210806150929 ◽

2021 ◽

Vol 16 ◽

Author(s):

Sepideh Khaleghi ◽

Fatemeh Rahbarizadeh ◽

Shahryar Khoshtinat Nikkhoi

Keyword(s):

Drug Delivery ◽

Real Time ◽

Targeted Delivery ◽

Cell Tracking ◽

Physicochemical Characterization ◽

Target Cells ◽

Wide Field ◽

Cell Trafficking ◽

Microscopy Imaging ◽

Intracellular Drug Delivery

Objective: The aim of this study was to formulate fluorescent-labeled targeted immunoliposome to visualize the delivery and distribution of drugs in real-time. Methods: In this study, fluorescent-labeled liposomes were decorated with anti-HER2 VHH or Herceptin to improve the monitoring of intracellular drug delivery and tumor cell tracking with minimal side effects. The conjugation efficiency of antibodies was analyzed by SDS-PAGE silver staining. In addition, the physicochemical characterization of liposomes was performed using DLS and TEM. Finally, confocal microscopy visualized nanoparticles in the target cells. Results: Quantitative and qualitative methods characterized the intracellular uptake of 110±10 nm particles with near 70% conjugation efficiency. In addition, live-cell trafficking during hours of incubation was monitored by wide-field microscopy imaging. The results show that the fluorescent-labeled nanoparticles can specifically bind to HER2-positive breast cancer with minimal off-target delivery. Conclusion: This kind of nanoparticles can have several applications in personalized medicine, especially drug delivery and real-time visualization of cancer therapy. Moreover, this method also can be applied in the targeted delivery of contrast agents in imaging and thermotherapy.

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Gold Nanoparticle-Based Fluorescent Theranostics for Real-Time Image-Guided Assessment of DNA Damage and Repair

International Journal of Molecular Sciences ◽

10.3390/ijms20030471 ◽

2019 ◽

Vol 20 (3) ◽

pp. 471 ◽

Cited By ~ 2

Author(s):

Shriya S. Srinivasan ◽

Rajesh Seenivasan ◽

Allison Condie ◽

Stanton L. Gerson ◽

Yanming Wang ◽

...

Keyword(s):

Molecular Imaging ◽

Real Time ◽

Targeted Delivery ◽

Specific Binding ◽

Imaging Techniques ◽

Cancer Cell Line ◽

Molecular Probe ◽

Biodistribution Studies

Chemotherapeutic dosing, is largely based on the tolerance levels of toxicity today. Molecular imaging strategies can be leveraged to quantify DNA cytotoxicity and thereby serve as a theranostic tool to improve the efficacy of treatments. Methoxyamine-modified cyanine-7 (Cy7MX) is a molecular probe which binds to apurinic/apyrimidinic (AP)-sites, inhibiting DNA-repair mechanisms implicated by cytotoxic chemotherapies. Herein, we loaded (Cy7MX) onto polyethylene glycol-coated gold nanoparticles (AuNP) to selectively and stably deliver the molecular probe intravenously to tumors. We optimized the properties of Cy7MX-loaded AuNPs using optical spectroscopy and tested the delivery mechanism and binding affinity using the DLD1 colon cancer cell line in vitro. A 10:1 ratio of Cy7MX-AuNPs demonstrated a strong AP site-specific binding and the cumulative release profile demonstrated 97% release within 12 min from a polar to a nonpolar environment. We further demonstrated targeted delivery using imaging and biodistribution studies in vivo in an xenografted mouse model. This work lays a foundation for the development of real-time molecular imaging techniques that are poised to yield quantitative measures of the efficacy and temporal profile of cytotoxic chemotherapies.

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Retinoic Acid Decorated Albumin-Chitosan Nanoparticles for Targeted Delivery of Doxorubicin Hydrochloride in Hepatocellular Carcinoma

Journal of Nanomaterials ◽

10.1155/2013/254127 ◽

2013 ◽

Vol 2013 ◽

pp. 1-12 ◽

Cited By ~ 7

Author(s):

Jaleh Varshosaz ◽

Farshid Hassanzadeh ◽

Hojjat Sadeghi ◽

Zahra Ghelich Khan ◽

Mahboobeh Rostami

Keyword(s):

Hepatocellular Carcinoma ◽

Retinoic Acid ◽

Targeted Delivery ◽

Drug Loading ◽

Chitosan Nanoparticles ◽

Free Drug ◽

Potential Drug ◽

Doxorubicin Hydrochloride ◽

Albumin Nanoparticles ◽

Successful Production

Retinoic acid (R) grafted chitosan (C) copolymers with different degree of substitution of retinoic acid on the chitosan were synthesized. Retinoic acid targeted chitosan-albumin nanoparticles were prepared for targeted delivery of doxorubicin in hepatocellular carcinoma by coacervation method. Physical properties of nanoparticles including particle size, zeta potential, drug loading efficiency, and drug release profiles were studied. TEM micrographs were taken to see the morphology of nanoparticles. The cytotoxicity of doxorubicin-loaded nanoparticles was studied on HepG2 cells using MTT assay and their cellular uptake by fluorescence microscopy. FTIR and1HNMR spectra confirmed successful production of RC conjugate which was used in production of the targeted RC-albumin nanoparticles. IC50of drug loaded in these nanoparticles reduced to half and one-third compared to nontargeted nanoparticles and free drug, respectively.

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Highly sensitive glutathione assay and intracellular imaging with functionalized semiconductor quantum dots

Nanoscale ◽

10.1039/c8nr09801h ◽

2019 ◽

Vol 11 (11) ◽

pp. 5014-5020 ◽

Cited By ~ 16

Author(s):

Junlin Sun ◽

Feng Liu ◽

Wenqian Yu ◽

Qunying Jiang ◽

Jialing Hu ◽

...

Keyword(s):

Quantum Dots ◽

Real Time ◽

Living Cells ◽

Semiconductor Quantum Dots ◽

Intracellular Imaging ◽

Fluorescent Nanoprobe ◽

Highly Sensitive

A fluorescent nanoprobe based on semiconductor quantum dots is designed for real-time glutathione imaging in living cells.

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Magnetic resonance–guided, real-time targeted delivery and imaging of magnetocapsules immunoprotecting pancreatic islet cells

Nature Medicine ◽

10.1038/nm1581 ◽

2007 ◽

Vol 13 (8) ◽

pp. 986-991 ◽

Cited By ~ 178

Author(s):

Brad P Barnett ◽

Aravind Arepally ◽

Parag V Karmarkar ◽

Di Qian ◽

Wesley D Gilson ◽

...

Keyword(s):

Magnetic Resonance ◽

Real Time ◽

Pancreatic Islet ◽

Targeted Delivery ◽

Islet Cells ◽

Pancreatic Islet Cells

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Glyco-Nanovesicles with Activatable Near-Infrared Probes for Real-Time Monitoring of Drug Release and Targeted Delivery

Chemistry of Materials ◽

10.1021/acs.chemmater.6b01857 ◽

2016 ◽

Vol 28 (12) ◽

pp. 4501-4506 ◽

Cited By ~ 32

Author(s):

Shoupeng Cao ◽

Zhichao Pei ◽

Yongqian Xu ◽

Yuxin Pei

Keyword(s):

Drug Release ◽

Real Time ◽

Targeted Delivery ◽

Near Infrared ◽

Real Time Monitoring ◽

Infrared Probes

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Redox-responsive xanthene–coumarin–chlorambucil-based FRET-guided theranostics for “activatable” combination therapy with real-time monitoring

Chemical Communications ◽

10.1039/c7cc03241b ◽

2017 ◽

Vol 53 (65) ◽

pp. 9109-9112 ◽

Cited By ~ 13

Author(s):

Moumita Gangopadhyay ◽

Rakesh Mengji ◽

Amrita Paul ◽

Yarra Venkatesh ◽

Venugopal Vangala ◽

...

Keyword(s):

Combination Therapy ◽

Real Time ◽

Real Time Monitoring ◽

Theranostic Agent ◽

Redox Responsive ◽

Fluorescence Change

A FRET-based theranostic agent, xanthene–coumarin–chlorambucil, exhibited redox-responsive “activatable” synergic treatment involving PDT and chemotherapy with fluorescence-change from green to blue.

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