Sample-Imprinted Polymer Potentially for Protein Depletion and Enrichment

2013 ◽  
Vol 3 (1) ◽  
pp. 40-45
Author(s):  
Chun Yang ◽  
Xinjie Luan ◽  
Meifeng Zhao ◽  
Qishu Qu ◽  
Xiaoya Hu
2019 ◽  
Vol 37 (4) ◽  
pp. 392
Author(s):  
Yuanyuan LI ◽  
Xin ZHANG ◽  
Weijie CHEN ◽  
Hongyang LIU ◽  
Liquan SUN ◽  
...  

2019 ◽  
Vol 25 (34) ◽  
pp. 3633-3644
Author(s):  
Nasrullah Shah ◽  
Saba Gul ◽  
Mazhar Ul-Islam

: Core-shell polymers represent a class of composite particles comprising of minimum two dissimilar constituents, one at the center known as a core which is occupied by the other called shell. Core-shell molecularly imprinting polymers (CSMIPs) are composites prepared via printing a template molecule (analyte) in the coreshell assembly followed by their elimination to provide the everlasting cavities specific to the template molecules. Various other types of CSMIPs with a partial shell, hollow-core and empty-shell are also prepared. Numerous methods have been reported for synthesizing the CSMIPs. CSMIPs composites could develop the ability to identify template molecules, increase the relative adsorption selectivity and offer higher adsorption capacity. Keen features are measured that permits these polymers to be utilized in numerous applications. It has been developed as a modern technique with the probability for an extensive range of uses in selective adsorption, biomedical fields, food processing, environmental applications, in utilizing the plant's extracts for further applications, and sensors. This review covers the approaches of developing the CSMIPs synthetic schemes, and their application with special emphasis on uses in the biomedical field, food care subjects, plant extracts analysis and in environmental studies.


2019 ◽  
Vol 15 (3) ◽  
pp. 251-257
Author(s):  
Bahareh Sadat Yousefsani ◽  
Seyed Ahmad Mohajeri ◽  
Mohammad Moshiri ◽  
Hossein Hosseinzadeh

Background:Molecularly imprinted polymers (MIPs) are synthetic polymers that have a selective site for a given analyte, or a group of structurally related compounds, that make them ideal polymers to be used in separation processes.Objective:An optimized molecularly imprinted polymer was selected and applied for selective extraction and analysis of clozapine in rat brain tissue.Methods:A molecularly imprinted solid-phase extraction (MISPE) method was developed for preconcentration and cleanup of clozapine in rat brain samples before HPLC-UV analysis. The extraction and analytical process was calibrated in the range of 0.025-100 ppm. Clozapine recovery in this MISPE process was calculated between 99.40 and 102.96%. The limit of detection (LOD) and the limit of quantification (LOQ) of the assay were 0.003 and 0.025 ppm, respectively. Intra-day precision values for clozapine concentrations of 0.125 and 0.025 ppm were 5.30 and 3.55%, whereas inter-day precision values of these concentrations were 9.23 and 6.15%, respectively. In this study, the effect of lipid emulsion infusion in reducing the brain concentration of drug was also evaluated.Results:The data indicated that calibrated method was successfully applied for the analysis of clozapine in the real rat brain samples after administration of a toxic dose to animal. Finally, the efficacy of lipid emulsion therapy in reducing the brain tissue concentration of clozapine after toxic administration of drug was determined.Conclusion:The proposed MISPE method could be applied in the extraction and preconcentration before HPLC-UV analysis of clozapine in rat brain tissue.


2020 ◽  
Vol 16 (4) ◽  
pp. 413-420 ◽  
Author(s):  
Youyuan Peng ◽  
Qiaolan Ji

Background: As a broad-spectrum antibiotic of the sulfonamide family, Sulfadimethoxine (SDM) has been widely utilized for therapeutic and growth-promoting purposes in animals. However, the use of SDM can cause residual problems. Even a low concentration of SDM in the aquatic system can exert toxic effects on target organisms and green algae. Therefore, the quantitation of SDM residues has become an important task. Methods: The present work describes the development of a sensitive and selective electrochemical sensor for sulfadimethoxine based on molecularly imprinted poly(o-aminophenol) film. The molecular imprinted polymer film was fabricated by electropolymerizing o-aminophenol in the presence of SDM after depositing carboxylfunctionalized multi-walled carbon nanotubes onto a glassy carbon electrode surface. SDM can be quickly removed by electrochemical methods. The imprinted polymer film was characterized by cyclic voltammetry, differential pulse voltammetry and scanning electron microscopy. Results: Under the selected optimal conditions, the molecularly imprinted sensor shows a linear range from 1.0 × 10-7 to 2.0 × 10-5 mol L-1 for SDM, with a detection limit of 4.0 × 10-8 mol L-1. The sensor was applied to the determination of SDM in aquaculture water samples successfully, with the recoveries ranging from 95% to 106%. Conclusion: The proposed sensor exhibited a high degree of selectivity for SDM in comparison to other structurally similar molecules, along with long-term stability, good reproducibility and excellent regeneration capacity. The sensor may offer a feasible strategy for the analysis of SDM in aquaculture water samples.


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