Effect ofOcimum tenuiflorumLinn Extract on Histamine Mediated Allergic Inflammation in Human Mast Cells

2017 ◽  
Vol 7 (1) ◽  
pp. 10-17
Author(s):  
Anupam Prakash ◽  
Angel Jemima Ebenezer ◽  
Sugitharini Vasanth ◽  
Gomathi Nagarajan ◽  
Berla Thangam Elden
Keyword(s):  
Mast Cells ◽  
Allergic Inflammation ◽  
Human Mast Cells

scholarly journals Mast cells and mastocytosis

Blood ◽  
2008 ◽  
Vol 112 (4) ◽  
pp. 946-956 ◽  
Author(s):  
Dean D. Metcalfe
Keyword(s):  
Mast Cells ◽  
Kinase Inhibitors ◽  
Allergic Inflammation ◽  
Host Responses ◽  
High Affinity ◽  
Human Mast Cells ◽  
Proinflammatory Mediators ◽  
High Affinity Receptor ◽  
Ige Mediated ◽  
Affinity Receptor

Abstract Mast cells have been recognized for well over 100 years. With time, human mast cells have been documented to originate from CD34+ cells, and have been implicated in host responses in both innate and acquired immunity. In clinical immunology, they are recognized for their central role in IgE-mediated degranulation and allergic inflammation by virtue of their expression of the high-affinity receptor for IgE and release of potent proinflammatory mediators. In hematology, the clinical disease of mastocytosis is characterized by a pathologic increase of mast cells in tissues, often associated with mutations in KIT, the receptor for stem cell factor. More recently, and with increased understanding of how human mast cells are activated through receptors including the high-affinity receptor for IgE and KIT, specific tyrosine kinase inhibitors have been identified with the potential to interrupt signaling pathways and thus limit the proliferation of mast cells as well as their activation through immunoglobulin receptors.

scholarly journals Phenolic Glycosides from Lindera obtusiloba and their Anti-allergic Inflammatory Activities

2013 ◽  
Vol 8 (2) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Hyun Gyu Choi ◽  
Hwa Dong Lee ◽  
Sang Hyun Kim ◽  
Min Kyun Na ◽  
Jeong Ah Kim ◽  
...  
Keyword(s):  
Mast Cells ◽  
Histamine Release ◽  
Proinflammatory Cytokines ◽  
Allergic Inflammation ◽  
Positive Control ◽  
Phenolic Glycosides ◽  
Gene Expressions ◽  
Human Mast Cells ◽  
Tnf Α ◽  
Lindera Obtusiloba

Eight phenolic glycosides, tachioside (1), isotachioside (2), koaburaside (3), 2,6-dimethoxy-4-hydroxyphenyl-1- O-ß-D-glucopyranoside (4), 4,6-dihydroxy-2- methoxyphenyl-1- O-β-D-glucopyranoside (5), a mixture of erigeside C (6a) and salidroside (6b), and 6-hydroxyphenyl)-1- O-β-D-glucopyranoside (7) were isolated from the stems of Lindera obtusiloba Blume. The structures of the isolates were determined by 1H-, 13C-NMR, COSY, HMQC, and HMBC spectroscopy. To evaluate their anti-allergic inflammatory activities, the inhibitory effects of isolates (1-7) on histamine release and on the gene expressions of tumor necrosis factor (TNF)-α and interleukin (IL)-6 were examined using human mast cells; previous studies have reported that TNF-α and IL-6 release from mast cells is positively related to the severity of allergic symptoms. Of the tested compounds, koaburaside (3), 2,6-dimethoxy-4-hydroxyphenyl-1- O-ß-D- glucopyranoside (4), and (6-hydroxyphenyl)-1- O-β-D-glucopyranoside (7) suppressed histamine release from mast cells as compared with gallic acid (positive control). In particular, 6-hydroxyphenyl)-1- O-β-D-glucopyranoside (7) attenuated the gene expressions of the proinflammatory cytokines TNF-α and IL-6 in human mast cells. Our results support the notion that phenolic glycosides isolated from L. obtusiloba inhibit mast-cell-derived allergic inflammation, histamine, and proinflammatory cytokines.

scholarly journals Topical Spilanthol Inhibits MAPK Signaling and Ameliorates Allergic Inflammation in DNCB-Induced Atopic Dermatitis in Mice

2019 ◽  
Vol 20 (10) ◽  
pp. 2490 ◽  
Author(s):  
Wen-Chung Huang ◽  
Chun-Hsun Huang ◽  
Sindy Hu ◽  
Hui-Ling Peng ◽  
Shu-Ju Wu
Keyword(s):  
Atopic Dermatitis ◽  
Mast Cells ◽  
Protein Kinase ◽  
Allergic Inflammation ◽  
Skin Lesions ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Mapk Pathways ◽  
Mitogen Activated Protein ◽  
Cox 2

Atopic dermatitis (AD) is a recurrent allergic skin disease caused by genetic and environmental factors. Patients with AD may experience immune imbalance, increased levels of mast cells, immunoglobulin (Ig) E and pro-inflammatory factors (Cyclooxygenase, COX-2 and inducible NO synthase, iNOS). While spilanthol (SP) has anti-inflammatory and analgesic activities, its effect on AD remains to be explored. To develop a new means of SP, inflammation-related symptoms of AD were alleviated, and 2,4-dinitrochlorobenzene (DNCB) was used to induce AD-like skin lesions in BALB/c mice. Histopathological analysis was used to examine mast cells and eosinophils infiltration in AD-like skin lesions. The levels of IgE, IgG1 and IgG2a were measured by enzyme-linked immunosorbent assay (ELISA) kits. Western blot was used for analysis of the mitogen-activated protein kinase (MAPK) pathways and COX-2 and iNOS protein expression. Topical SP treatment reduced serum IgE and IgG2a levels and suppressed COX-2 and iNOS expression via blocked mitogen-activated protein kinase (MAPK) pathways in DNCB-induced AD-like lesions. Histopathological examination revealed that SP reduced epidermal thickness and collagen accumulation and inhibited mast cells and eosinophils infiltration into the AD-like lesions skin. These results indicate that SP may protect against AD skin lesions through inhibited MAPK signaling pathways and may diminish the infiltration of inflammatory cells to block allergic inflammation.

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