scholarly journals Local infusion of the nitric oxide donor sin-1 after angioplasty: Effects on intimal hyperplasia in porcine coronary arteries

2003 ◽  
Vol 44 (4) ◽  
pp. 395-402
Author(s):  
J. Harnek ◽  
E. Zoucas ◽  
R. Sjuve ◽  
A. Arner ◽  
E. Ekblad ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Arteries ◽  
Intimal Hyperplasia ◽  
Percutaneous Transluminal Coronary Angioplasty ◽  
Local Delivery ◽  
Nitric Oxide Donor ◽  
Histological Evaluation ◽  
No Donor ◽  
Luminal Diameter ◽  
Transluminal Coronary Angioplasty

Purpose: To investigate the development of intimal hyperplasia in response to percutaneous transluminal coronary angioplasty (PTCA) followed by local delivery of the nitric oxide (NO) donor 3-morpholino-sydnonimine (SIN-1). Material and Methods: Overdilation PTCA was performed in coronary arteries in 20 healthy pigs. One of the dilated segments was additionally treated with local delivery of SIN-1 for 10 min. Segments distal to the treated part of the arteries served as controls. Arteries were radiographically depicted and analyzed after 1 and 8 weeks for actin, myosin and intermediate filaments (IF), nitric oxide synthetase (NOS) and histological evaluation. Results: Segments treated with PTCA+SIN-1 showed a significantly ( p = 0.03) larger luminal diameter compared with PTCA only treated segments. The luminal loss after SIN-1 was not significant compared with the diameter prior to treatment. Endothelial NOS content was significantly lower in the PTCA+SIN-1 group compared with the PTCA group after 1 ( p = 0.03) and 8 weeks ( p = 0.013). IF/actin ratio after 1 week was significantly increased in PTCA-treated segments compared with untreated controls ( p = 0.004), and compared with PTCA+SIN-1-treated segments ( p = 0.004). Conclusion: PTCA-induced intimal hyperplasia was potently inhibited by local delivery of the NO donor SIN-1. Momentary events at the time of injury play a significant role in the development of intimal hyperplasia and long-lasting down-regulation of the endothelial NOS expression after SIN-1 exposure is suggested. The IF/actin ratio can be useful as an early marker of intimal hyperplasia.

scholarly journals Local Infusion of the Nitric oxide Donor Sin-1 After Angioplasty . Effects on intimal hyperplasia in porcine coronary arteries

2003 ◽  
Vol 44 (4) ◽  
pp. 395-402 ◽  
Author(s):  
J. Harnek ◽  
E. Zoucas ◽  
R. Sjuve ◽  
A. Arner ◽  
E. Ekblad ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Coronary Arteries ◽  
Intimal Hyperplasia ◽  
Nitric Oxide Donor

Acute cerebral vascular injury after subarachnoid hemorrhage and its prevention by administration of a nitric oxide donor

2007 ◽  
Vol 106 (2) ◽  
pp. 321-329 ◽  
Author(s):  
Fatima A. Sehba ◽  
Victor Friedrich ◽  
Girma Makonnen ◽  
Joshua B. Bederson
Keyword(s):  
Nitric Oxide ◽  
Subarachnoid Hemorrhage ◽  
Structural Changes ◽  
Collagen Iv ◽  
Nitric Oxide Donor ◽  
Vascular Structure ◽  
Collagenase Activity ◽  
No Donor ◽  
Luminal Diameter ◽  
Brain Parenchymal

Object Structural changes in brain parenchymal vessels occur within minutes after subarachnoid hemorrhage (SAH). These changes include platelet aggregation, activation of vascular collagenases, and destruction of perivascular collagen IV. Because collagen IV is an important component of the basal lamina, the authors attempted to further define changes in vascular structure (length and luminal diameter) and their relationship to vascular permeability immediately after SAH. In addition, the authors explored whether such alterations were attenuated by administration of a nitric oxide (NO) donor. Methods Endovascular perforation was used to induce SAH in rats. Two sets of experiments were performed. The first established changes in vascular structure and permeability (collagen IV and endothelial barrier antigen [EBA] dual immunofluorescence) during the first 24 hours after SAH. In the second, the investigators examined the effects of an NO donor on vascular structure, permeability, and collagenase activity (in situ zymography). In this second study, animals received intravenous infusion of the NO donor S-nitrosoglutathione (GSNO, 1 μM/8 μl/min) 15 minutes after induction of SAH and were killed 3 hours after SAH onset. Controls were naive unoperated animals for the first study and saline-infused SAH animals for the second. The authors found a time-dependent decrease in area fraction, length, and luminal diameter of collagen IV– and EBA-immunofluorescent vessels after SAH. The greatest change occurred at 3 hours after onset of SAH. Administration of GSNO was associated with striking preservation of collagen IV and EBA immunofluorescence compared with saline treatment. Zymography indicated decreased collagenase activity in GSNO-treated SAH animals compared with saline-treated SAH animals. Conclusions These results demonstrate changes in the structure and permeability of brain parenchymal microvessels after SAH and their reversal by treatment with an NO donor.

scholarly journals Intimal Hyperplasia in Balloon Dilated Coronary Arteries is Reduced by Local Delivery of the NO Donor, SIN-1 Via a cGMP-Dependent Pathway

2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Jan Harnek ◽  
Evita Zoucas ◽  
Valéria Perez de Sá ◽  
Eva Ekblad ◽  
Anders Arner ◽  
...  
Keyword(s):  
Coronary Arteries ◽  
Intimal Hyperplasia ◽  
Local Delivery ◽  
No Donor ◽  
Dependent Pathway

Skeletal muscle force and actomyosin ATPase activity reduced by nitric oxide donor

1997 ◽  
Vol 83 (4) ◽  
pp. 1326-1332 ◽  
Author(s):  
William J. Perkins ◽  
Young-Soo Han ◽  
Gary C. Sieck
Keyword(s):  
Nitric Oxide ◽  
Mechanical Properties ◽  
Atpase Activity ◽  
Muscle Force ◽  
Isometric Force ◽  
Nitric Oxide Donor ◽  
No Donor ◽  
Single Fibers ◽  
Actomyosin Atpase ◽  
Cross Bridge

Perkins, William J., Young-Soo Han, and Gary C. Sieck.Skeletal muscle force and actomyosin ATPase activity reduced by nitric oxide donor. J. Appl. Physiol.83(4): 1326–1332, 1997.—Nitric oxide (NO) may exert direct effects on actin-myosin cross-bridge cycling by modulating critical thiols on the myosin head. In the present study, the effects of the NO donor sodium nitroprusside (SNP; 100 μM to 10 mM) on mechanical properties and actomyosin adenosinetriphosphatase (ATPase) activity of single permeabilized muscle fibers from the rabbit psoas muscle were determined. The effects of N-ethylmaleimide (NEM; 5–250 μM), a thiol-specific alkylating reagent, on mechanical properties of single fibers were also evaluated. Both NEM (≥25 μM) and SNP (≥1 mM) significantly inhibited isometric force and actomyosin ATPase activity. The unloaded shortening velocity of SNP-treated single fibers was decreased, but to a lesser extent, suggesting that SNP effects on isometric force and actomyosin ATPase were largely due to decreased cross-bridge recruitment. The calcium sensitivity of SNP-treated single fibers was also decreased. The effects of SNP, but not NEM, on force and actomyosin ATPase activity were reversed by treatment with 10 mMdl-dithiothreitol, a thiol-reducing agent. We conclude that the NO donor SNP inhibits contractile function caused by reversible oxidation of contractile protein thiols.

scholarly journals Dopamine in the ink defence system of Sepia officinalis: biosynthesis, vesicular compartmentation in mature ink gland cells, nitric oxide (NO)/cGMP-induced depletion and fate in secreted ink1

2004 ◽  
Vol 378 (3) ◽  
pp. 785-791 ◽  
Author(s):  
Gabriella FIORE ◽  
Annarita POLI ◽  
Anna Di COSMO ◽  
Marco d'ISCHIA ◽  
Anna PALUMBO
Keyword(s):  
Nitric Oxide ◽  
Tyrosine Hydroxylase ◽  
Physical Adsorption ◽  
Signalling Pathway ◽  
Nitric Oxide Donor ◽  
Sepia Officinalis ◽  
Release Process ◽  
No Donor ◽  
Cgmp Signalling ◽  
Incubation Experiments

The biosynthesis, localization and fate of catecholamines in the ink gland of the cuttlefish Sepia officinalis were investigated by combined biochemical and immunohistocytochemical methodologies. HPLC analysis of crude ink gland extracts indicated the presence of dopa (2.18±0.82 nmol/mg of protein) and DA (dopamine, 0.06±0.02 nmol/mg of protein), but no detectable noradrenaline or adrenaline. DA was shown to derive from l-tyrosine, according to experiments performed by incubating intact ink glands with [l-14C]tyrosine. The biosynthetic process involves a tyrosine hydroxylase and a dopa decarboxylase pathway and is independent of tyrosinase. The tyrosine hydroxylase activity was detected under conditions of tyrosinase suppression in the cytosolic fraction, but not in the melanosomal fraction, of ink gland extracts, and the presence of the enzyme was confirmed by Western-blot analysis. Dopa and DA were found to be released from the ink glands by processes controlled through the NMDA-nitric oxide-cGMP (where NMDA stands for N-methyl-d-aspartate) signalling pathway, as apparent from incubation experiments performed with [l-14C]tyrosine in the presence of NMDA, diethylamine NONOate (diethylamine diazeniumdiolate), a nitric oxide donor, 8-bromo-cGMP or a guanylyl cyclase inhibitor. Immunohistochemical results coupled with electron microscopy indicated that DA was concentrated in vesicles specifically localized in the mature melanin-producing cells of the ink gland proximal to the lumen and separated from the melanin-containing melanosomes. NMDA receptor stimulation or exposure to an NO donor caused a marked loss of DA immunoreactivity in mature cells, consistent with a release process. In the lumen of the ink gland, where mature exhausted cells pour their contents, DA immunoreactivity was found to be associated with the melanin granules, due apparently to physical adsorption. Overall, these results point to DA as a marker of cell maturation in Sepia ink gland subject to release by the NO/cGMP signalling pathway, and disclose apparently overlooked DA–melanin interactions in secreted ink of possible relevance to the defence mechanism.

scholarly journals Synthesis and characterization of a fluorinated S-nitrosothiol as the nitric oxide donor for fluoropolymer-based biomedical device applications

2018 ◽  
Vol 6 (38) ◽  
pp. 6142-6152 ◽  
Author(s):  
Yang Zhou ◽  
Qi Zhang ◽  
Jianfeng Wu ◽  
Chuanwu Xi ◽  
Mark E. Meyerhoff
Keyword(s):  
Nitric Oxide ◽  
Polyvinylidene Fluoride ◽  
Nitric Oxide Donor ◽  
Synthesis And Characterization ◽  
No Donor ◽  
Fluorinated Polymer ◽  
Biomedical Device ◽  
Device Applications

The first nitric oxide (NO) releasing fluorinated polymer was developed via incorporating a new fluorinated NO donor into polyvinylidene fluoride tubing.

Nitric oxide donor stimulated increase of cyclic GMP in the goldfish retina

2001 ◽  
Vol 18 (6) ◽  
pp. 849-856 ◽  
Author(s):  
WILLIAM H. BALDRIDGE ◽  
ANDY J. FISCHER
Keyword(s):  
Nitric Oxide ◽  
Optic Nerve ◽  
Ganglion Cells ◽  
Soluble Guanylyl Cyclase ◽  
Cyclic Guanosine Monophosphate ◽  
Guanosine Monophosphate ◽  
Nitric Oxide Donor ◽  
No Donor ◽  
Intracellular Signalling Pathway ◽  
Teleost Retina

Nitric oxide (NO) activates soluble guanylyl cyclase (sGC) and the resulting increase in cyclic guanosine monophosphate (cGMP) is an important intracellular signalling pathway in the vertebrate retina. Immunocytochemical detection of cGMP following exposure to NO donors has proven an effective method of identifying cells that express sGC. While such an approach has proven useful for the study of several vertebrate retinas, it has not been applied to the well-characterized teleost retina. Therefore, in the present study, we have applied this approach to the retina of the goldfish (Carassius auratus). In the presence of the phosphodiesterase (PDE) inhibitor 3-isobutyl-1-methylxanthine (IBMX), incubation of goldfish eyecups in Ringer's solution containing (±)-S-nitroso-N-acetylpenicillamine (SNAP) increased cGMP-like immunoreactivity (cG-ir) in bipolar, horizontal, amacrine, and ganglion cells and in ganglion cell axons and optic nerve. Weak labeling was observed in horizontal cells but no change in cG-ir was noted within photoreceptors. The NO donor-stimulated increases of cG-ir in horizontal, bipolar, amacrine, and ganglion cells are consistent with known physiological effects of NO on these neurons. The physiological significance of NO action at the level of optic nerve is not known. The lack of an effect of SNAP on cG-ir in photoreceptors was unexpected, as there are known physiological actions of NO, mediated by cGMP, on these neurons. Although this may be due to insufficient sensitivity of immunolabeling, this result may indicate a difference between isoforms of sGC or cGMP PDE in these neurons, compared to neurons where exogenous NO increased cG-ir.

scholarly journals Percutaneous Transluminal Coronary Angioplasty (PTCA) and Stenting – Study of 100 Cases

1970 ◽  
Vol 26 (1) ◽  
pp. 26-31
Author(s):  
F Rahman ◽  
S Banerjee ◽  
CM Ahmed ◽  
MS Uddin ◽  
Khirul Anam ◽  
...  
Keyword(s):  
Coronary Artery ◽  
Coronary Angioplasty ◽  
Percutaneous Transluminal Coronary Angioplasty ◽  
Clinical Results ◽  
Left Ventricular ◽  
Left Ventricular Ejection ◽  
Short Term ◽  
Luminal Diameter ◽  
Transluminal Coronary Angioplasty ◽  
Percutaneous Transluminal

This prospective ongoing study conducted in University Cardiac Center, BSMMU, Dhaka from July 2004 to April 2006. 100 patients (mean age 52.4±6.2 years) underwent Percutaneous Transluminal Coronary angioplasty and stenting (PTCA & stenting) were evaluated. This study was designed to evaluate the short term angiographic and clinical results of stentangioplasty during hospital stay. The study group of 100 patients consisted of 88 (88%) men and 12 (12%) women. About risk factors 36 (36%) had hypertension, 30 (30%) were smoker, 20 (20%) suffered from diabetes mellitus, 14 (14%) had family history of ischaemic heart disease. Average Left ventricular ejection fraction was 54.2±7. Target vessel PTCA were done on 130 vessels, intracoronary stent implanted in 124 vessels, direct stenting was done in 80 cases, failed PTCA were in 4 (4%) cases, and three patients had dissection. The native vessels had a mean reference diameter of 2.89 mm and their luminal diameter increased significantly after percutaneous coronary intervention (PCI). Thombolysis in myocardial infarction (TIMI) flow analysis showed most of the patients had TIMI-1 flow (95,73%) before the procedure and maximum patients achieved TIMI-3 flow (91, 70%) after the procedure with significant clinical improvement. All the patients were discharged by one to three days of the procedure with improvement of their clinical condition. So PTCA and Stenting is a safe and effective technique with high procedural success rate and good short-term (hospital) clinical results in the native coronary artery lesions. Key words: Coronary artery diseases; PTCA and stenting. DOI: 10.3329/jbcps.v26i1.4230 J Bangladesh Coll Phys Surg 2008; 26: 26-31

Sign in / Sign up

Export Citation Format

Share Document