Nucleotide Degradation Products in Cerebrospinal Fluid (CSF) in Inherited and Acquired Pathologies

2004 ◽  
Vol 23 (8-9) ◽  
pp. 1185-1187 ◽  
Author(s):  
L. D. Fairbanks ◽  
J. C. Harris ◽  
J. A. Duley ◽  
H. A. Simmonds
Keyword(s):  
Cerebrospinal Fluid ◽  
Degradation Products ◽  
Nucleotide Degradation

HPLC method for measurement of purine nucleotide degradation products in cerebrospinal fluid

1996 ◽  
Vol 42 (5) ◽  
pp. 756-760 ◽  
Author(s):  
L Kuracka ◽  
T Kalnovicová ◽  
B Líska ◽  
P Turcáni
Keyword(s):  
Cerebrospinal Fluid ◽  
Uric Acid ◽  
Neurological Disorders ◽  
Limit Of Detection ◽  
Degradation Products ◽  
Potassium Phosphate ◽  
Hplc Method ◽  
Potassium Phosphate Buffer ◽  
Nucleotide Degradation

Abstract We describe a convenient method for the separation and quantification of xanthine, hypoxanthine, and uric acid in 20 microL of cerebrospinal fluid (CSF) with use of HPLC and ultraviolet detection. The analysis is performed on a Sepharon SGX C18 column and the elution system consists of potassium phosphate buffer, pH 5.1, with 20 mL/L methanol. The lower limit of detection was 4 pmol for hypoxanthine and xanthine and 6 pmol for uric acid. Analytical recoveries of purine metabolites ranged from 98.6% to 102.9%. The intra- and interassay CVs were <3%. The applicability of the method is illustrated with the determination of micromolar concentrations of xanthine, hypoxanthine, and uric acid in CSF samples obtained from 113 patients with various neurological disorders.

Nucleotide Degradation Products in Cerebrospinal Fluid (CSF) in Inherited and Acquired Pathologies

ChemInform ◽  
2005 ◽  
Vol 36 (9) ◽  
Author(s):  
L. D. Fairbanks ◽  
J. C. Harris ◽  
J. A. Duley ◽  
H. A. Simmonds
Keyword(s):  
Cerebrospinal Fluid ◽  
Degradation Products ◽  
Nucleotide Degradation

scholarly journals Increased Adenine Nucleotide Degradation in Skeletal Muscle Atrophy

2019 ◽  
Vol 21 (1) ◽  
pp. 88 ◽  
Author(s):  
Spencer G. Miller ◽  
Paul S. Hafen ◽  
Jeffrey J. Brault
Keyword(s):  
Skeletal Muscle ◽  
Muscle Atrophy ◽  
Intracellular Signaling ◽  
Adenine Nucleotide ◽  
Degradation Products ◽  
Adenine Nucleotides ◽  
Skeletal Muscle Atrophy ◽  
Valuable Insight ◽  
Disease States ◽  
Nucleotide Degradation

Adenine nucleotides (AdNs: ATP, ADP, AMP) are essential biological compounds that facilitate many necessary cellular processes by providing chemical energy, mediating intracellular signaling, and regulating protein metabolism and solubilization. A dramatic reduction in total AdNs is observed in atrophic skeletal muscle across numerous disease states and conditions, such as cancer, diabetes, chronic kidney disease, heart failure, COPD, sepsis, muscular dystrophy, denervation, disuse, and sarcopenia. The reduced AdNs in atrophic skeletal muscle are accompanied by increased expression/activities of AdN degrading enzymes and the accumulation of degradation products (IMP, hypoxanthine, xanthine, uric acid), suggesting that the lower AdN content is largely the result of increased nucleotide degradation. Furthermore, this characteristic decrease of AdNs suggests that increased nucleotide degradation contributes to the general pathophysiology of skeletal muscle atrophy. In view of the numerous energetic, and non-energetic, roles of AdNs in skeletal muscle, investigations into the physiological consequences of AdN degradation may provide valuable insight into the mechanisms of muscle atrophy.

scholarly journals Biochemical Response to Freezing in the Siberian Salamander Salamandrella keyserlingii

Biology ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1172
Author(s):  
Sergei V. Shekhovtsov ◽  
Nina A. Bulakhova ◽  
Yuri P. Tsentalovich ◽  
Ekaterina A. Zelentsova ◽  
Ekaterina N. Meshcheryakova ◽  
...  
Keyword(s):  
Degradation Products ◽  
Ice Formation ◽  
Low Molecular Weight ◽  
Biochemical Basis ◽  
Hindlimb Muscle ◽  
Adenosine Phosphate ◽  
Nucleotide Degradation ◽  
Quantitative Changes ◽  
Siberian Salamander

The Siberian salamander Salamandrella keyserlingii Dybowski, 1870 is a unique amphibian that is capable to survive long-term freezing at −55 °C. Nothing is known on the biochemical basis of this remarkable freezing tolerance, except for the fact that it uses glycerol as a low molecular weight cryoprotectant. We used 1H-NMR analysis to study quantitative changes of multiple metabolites in liver and hindlimb muscle of S. keyserlingii in response to freezing. For the majority of molecules we observed significant changes in concentrations. Glycerol content in frozen organs was as high as 2% w/w, which confirms its role as a cryoprotectant. No other putative cryoprotectants were detected. Freezing resulted in ischemia manifested as increased concentrations of glycolysis products: lactate and alanine. Unexpectedly, we detected no increase in concentrations of succinate, which accumulates under ischemia in various tetrapods. Freezing proved to be a dramatic stress with reduced adenosine phosphate pool and high levels of nucleotide degradation products (hypoxanthine, β-alanine, and β-aminoisobutyrate). There was also significant increase in the concentrations of choline and glycerophosphocholine, which may be interpreted as the degradation of biomembranes. Thus, we found that freezing results not only in macroscopical damage due to ice formation, but also to degradation of DNA and biomembranes.

scholarly journals Comparison of glucose, lactate, and nucleotide degradation products content of cooked Hanwoo and Australian beef steaks by internal temperature

2015 ◽  
Vol 42 (4) ◽  
pp. 369-374
Author(s):  
Sun-Moon Kang ◽  
Geun-Ho Kang ◽  
Pil-Nam Seong ◽  
Young-Chun Kim ◽  
Jin-Hyoung Kim ◽  
...  
Keyword(s):  
Degradation Products ◽  
Internal Temperature ◽  
Beef Steaks ◽  
Nucleotide Degradation
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