scholarly journals The microRNA-200 family coordinately regulates cell adhesion and proliferation in hair morphogenesis

2018 ◽  
Vol 217 (6) ◽  
pp. 2185-2204 ◽  
Author(s):  
Jaimee E. Hoefert ◽  
Glen A. Bjerke ◽  
Dongmei Wang ◽  
Rui Yi
Keyword(s):  
Cell Adhesion ◽  
Cell Fate ◽  
Mirna Family ◽  
Metastatic Cancer ◽  
Seed Region ◽  
Cell Fate Specification ◽  
Single Nucleotide ◽  
Target Sites ◽  
Hair Morphogenesis ◽  
Cell Adhesion And Proliferation

The microRNA (miRNA)-200 (miR-200) family is highly expressed in epithelial cells and frequently lost in metastatic cancer. Despite intensive studies into their roles in cancer, their targets and functions in normal epithelial tissues remain unclear. Importantly, it remains unclear how the two subfamilies of the five-miRNA family, distinguished by a single nucleotide within the seed region, regulate their targets. By directly ligating miRNAs to their targeted mRNA regions, we identify numerous miR-200 targets involved in the regulation of focal adhesion, actin cytoskeleton, cell cycle, and Hippo/Yap signaling. The two subfamilies bind to largely distinct target sites, but many genes are coordinately regulated by both subfamilies. Using inducible and knockout mouse models, we show that the miR-200 family regulates cell adhesion and orientation in the hair germ, contributing to precise cell fate specification and hair morphogenesis. Our findings demonstrate that combinatorial targeting of many genes is critical for miRNA function and provide new insights into miR-200’s functions.

scholarly journals β-catenin has an ancestral role in cell fate specification but not cell adhesion

2019 ◽  
Author(s):  
Miguel Salinas-Saavedra ◽  
Athula H. Wikramanayake ◽  
Mark Q Martindale
Keyword(s):  
Cell Adhesion ◽  
Cell Fate ◽  
Target Genes ◽  
Critical Role ◽  
Mnemiopsis Leidyi ◽  
Epithelial Tissue ◽  
Cell Fate Specification ◽  
Animal Cells ◽  
Fate Specification

AbstractThe ß-catenin protein has two major known functions in animal cells. It keeps epithelial tissue homeostasis by its connection with Adherens Junctions (AJ), and it serves as a transcriptional cofactor along with Lef/Tcf to enter the nucleus and regulate target genes of the Wnt/ß-catenin (cWnt) signaling pathway. To assess the ancestral role of ß-catenin during development we examined its distribution and function in the ctenophore Mnemiopsis leidyi (one of the earliest branching animal phyla) by using ctenophore-specific antibodies and mRNA injection. We found that ß-catenin protein never localizes to cell-cell contacts during embryogenesis as it does in other metazoans, most likely because ctenophore-cadherins do not have the cytoplasmic domain required for interaction with the catenin proteins. Downregulation of zygotic Mlß-catenin signaling led to the loss of endodermal and mesodermal tissues indicating that nuclear ß-catenin may have a deep role in germ-layer evolution. Our results indicate that the ancestral role for ß-catenin was in the cell-fate specification and not in cell adhesion and also further emphasizes the critical role of this protein in the evolution of tissue layers in metazoans.

Silk-based hybrid microfibrous mats as guided bone regeneration membranes

2021 ◽  
Author(s):  
Mi Wu ◽  
Zhengyi Han ◽  
Wen Liu ◽  
Jinrong Yao ◽  
Bingjiao Zhao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Cell Adhesion ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Guided Bone Regeneration ◽  
Regenerated Silk Fibroin ◽  
Marrow Mesenchymal Stem Cells ◽  
Cell Adhesion And Proliferation

LAPONITE® (LAP) nanoplatelets were incorporated within a regenerated silk fibroin (RSF) microfibrous mat via electrospinning, which exhibited better cell adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) than the pristine RSF ones.

Sox proteins: regulators of cell fate specification and differentiation

Development ◽  
2013 ◽  
Vol 140 (20) ◽  
pp. 4129-4144 ◽  
Author(s):  
Y. Kamachi ◽  
H. Kondoh
Keyword(s):  
Cell Fate ◽  
Cell Fate Specification ◽  
Fate Specification ◽  
Sox Proteins

Effect of Surface Potential on NIH3T3 Cell Adhesion and Proliferation

2014 ◽  
Vol 118 (26) ◽  
pp. 14464-14470 ◽  
Author(s):  
Hsun-Yun Chang ◽  
Chih-Chieh Huang ◽  
Kang-Yi Lin ◽  
Wei-Lun Kao ◽  
Hua-Yang Liao ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Surface Potential ◽  
Nih3t3 Cell ◽  
Cell Adhesion And Proliferation ◽  
Effect Of Surface

scholarly journals Surface functionalisation of nanodiamonds for human neural stem cell adhesion and proliferation

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Alice C. Taylor ◽  
Citlali Helenes González ◽  
Benjamin S. Miller ◽  
Robert J. Edgington ◽  
Patrizia Ferretti ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Adhesion ◽  
Neural Stem Cell ◽  
Human Neural Stem Cell ◽  
Surface Functionalisation ◽  
Cell Adhesion And Proliferation

scholarly journals Three-Dimensional Bone Substitutes for Oral and Maxillofacial Surgery: Biological and Structural Characterization

2018 ◽  
Vol 9 (4) ◽  
pp. 62 ◽  
Author(s):  
Gianluca Turco ◽  
Davide Porrelli ◽  
Eleonora Marsich ◽  
Federica Vecchies ◽  
Teresa Lombardi ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Clinical Performance ◽  
Maxillofacial Surgery ◽  
Chemical Characterization ◽  
Bone Substitutes ◽  
Human Cell Line ◽  
Structural Features ◽  
Oral And Maxillofacial Surgery ◽  
Cell Adhesion And Proliferation

Background: Bone substitutes, either from human (autografts and allografts) or animal (xenografts) sources, suffer from inherent drawbacks including limited availability or potential infectivity to name a few. In the last decade, synthetic biomaterials have emerged as a valid alternative for biomedical applications in the field of orthopedic and maxillofacial surgery. In particular, phosphate-based bone substitution materials have exhibited a high biocompatibility due to their chemical similitude with natural hydroxyapatite. Besides the nature of the biomaterial, its porous and interconnected architecture is essential for a correct osseointegration. This performance could be predicted with an extensive characterization of the biomaterial in vitro. Methods: In this study, we compared the biological, chemical, and structural features of four different commercially available bone substitutes derived from an animal or a synthetic source. To this end, µ-CT and SEM were used to describe the biomaterials structure. Both FTIR and EDS analyses were carried out to provide a chemical characterization. The results obtained by these techniques were correlated with cell adhesion and proliferation of the osteosarcoma MG-63 human cell line cultured in vitro. Results: The findings reported in this paper indicate a significant influence of both the nature and the structure of the biomaterials in cell adhesion and proliferation, which ultimately could affect the clinical performance of the biomaterials. Conclusions: The four commercially available bone substitutes investigated in this work significantly differed in terms of structural features, which ultimately influenced in vitro cell proliferation and may so affect the clinical performance of the biomaterials.

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