scholarly journals EFFECTS OF ARGININE DEPRIVATION, ULTRAVIOLET RADIATION, AND X-RADIATION ON CULTURED KB CELLS

1965 ◽  
Vol 27 (3) ◽  
pp. 603-620 ◽  
Author(s):  
Nancy J. Lane ◽  
Alex B. Novikoff
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Ultraviolet Radiation ◽  
Light And Electron Microscopy ◽  
Multivesicular Bodies ◽  
Kb Cells ◽  
Nucleolar Structure ◽  
Aryl Sulfatase ◽  
Arginine Deprivation ◽  
High Doses

Cultured KB cells (derived from a human oral carcinoma) grown in monolayers were injured by one of three agents: starvation by arginine deprivation or treatment with high doses of either ultraviolet radiation or x-radiation. The different agents produced changes in nucleolar structure and varying accumulations of triglyceride and glycogen. All three agents produced an increase in number and size of lysosomes. These were studied in acid phosphatase preparations, viewed by both light and electron microscopy, and, occasionally, in vital dye, esterase, and aryl sulfatase preparations. Ultrastructurally, alterations in lysosomes suggested that "residual bodies" developed in a variety of ways, i.e., from the endoplasmic reticulum, multivesicular bodies, or autophagic vacuoles. Following all three agents the endoplasmic reticulum assumed the form of "rough" or "smooth" whorls, and, after two of the agents, arginine deprivation or ultraviolet radiation, it acquired cytochemically demonstrable acid phosphatase activity. Near connections between the endoplasmic reticulum and lysosomes raise the possibility that in KB cells, at least when injured, the endoplasmic reticulum is involved in the formation of lysosomes and the transport of acid phosphatase to them.

Formation of chlamydospores in Gilbertella persicaria

1981 ◽  
Vol 59 (5) ◽  
pp. 908-928 ◽  
Author(s):  
Martha J. Powell ◽  
Charles E. Bracker ◽  
David J. Sternshein
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Acid Phosphatase ◽  
Light And Electron Microscopy ◽  
Multivesicular Bodies ◽  
Marker Enzyme ◽  
Transparent Layer ◽  
Thiamine Pyrophosphatase ◽  
Gilbertella Persicaria

The cytological events involved in the transformation of vegetative hyphae of the zygomycete Gilbertella persicaria (Eddy) Hesseltine into chlamydospores were studied with light and electron microscopy. Thirty hours after sporangiospores were inoculated into YPG broth, swellings appeared along the aseptate hyphae. Later, septa, traversed by plasmodesmata, delimited each end of the hyphal swellings and compartmentalized these hyphal regions as they differentiated into chlamydospores. Nonswollen regions adjacent to chlamydospores remained as isthmuses. Two additional wall layers appeared within the vegetative wall of the developing chlamydospores. An alveolate, electron-dense wall formed first, and then an electron-transparent layer containing concentrically oriented fibers formed between this layer and the plasma membrane. Rather than a mere condensation of cytoplasm, development and maturation of the multinucleate chlamydospores involved extensive cytoplasmic changes such as an increase in reserve products, lipid and glycogen, an increase and then disappearance of vacuoles, and the breakdown of many mitochondria. Underlying the plasma membrane during chlamydospore wall formation were endoplasmic reticulum, multivesicular bodies, vesicles with fibrillar contents, vesicles with electron-transparent contents, and cisternal rings containing the Golgi apparatus marker enzyme, thiamine pyrophosphatase. Acid phosphatase activity was localized cytochemically in a cisterna which enclosed mitochondria and in vacuoles which contained membrane fragments. Tightly packed membrane whorls and single membrane bounded sacs with finely granular matrices surrounding vacuoles were unique during chlamydospore development. Microbodies were rare in the mature chlamydospore, but endoplasmic reticulum was closely associated with lipid globules. As chlamydospores developed, the cytoplasm in the isthmus became highly vacuolated, lipid globules were closely associated with vacuoles, mitochondria were broken down in vacuoles, unusual membrane configurations appeared, and eventually the membranes degenerated. Unlike chlamydospores, walls of the isthmus did not thicken, but irregularly shaped appositions containing numerous channels formed at intervals on the inside of these walls. The pattern of cytoplasmic transformations during chlamydospore development is similar to events leading to the formation of zygospores and sporangiospores.

scholarly journals CYTOCHEMICAL STUDIES OF LYSOSOMES, GOLGI APPARATUS AND ENDOPLASMIC RETICULUM IN SECRETION AND PROTEIN UPTAKE BY ADRENAL MEDULLA CELLS OF THE RAT

1968 ◽  
Vol 16 (5) ◽  
pp. 320-336 ◽  
Author(s):  
ERIC HOLTZMAN ◽  
REGINA DOMINITZ
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Golgi Apparatus ◽  
Adrenal Medulla ◽  
Secretory Granules ◽  
Light And Electron Microscopy ◽  
Multivesicular Bodies ◽  
Frozen Sections ◽  
Protein Uptake ◽  
Thiamine Pyrophosphatase

The adrenalin-producing cells of the rat adrenal medulla have been studied by light and electron microscopy. Frozen sections of glutaraldehyde-perfused material were incubated for demonstration of "marker" enzymes for lysosomes (acid phosphatase, aryl sulfatase) and Golgi apparatus (thiamine pyrophosphatase). In addition, the uptake and fate of intravenously administered horseradish peroxidase was followed. Acid phosphatase activity is demonstrable in secretory granules, Golgi saccules, vesicles in the Golgi area and in the agranular tubules and cisternae (GERL) from which secretory granules appear to form at the inner surface of the Golgi apparatus. Endoplasmic reticulum with ribosomes on only one surface is closely apposed to both inner and outer aspects of the Golgi apparatus. Peroxidase is taken up in vesicles, tubules and "cup-like" bodies. The latter apparently transform into multivesicular bodies. A possible source of the acid phosphatase found in multivesicular bodies is the small vesicles from the Golgi apparatus or GERL.

scholarly journals LOCALIZATION OF PEROXIDASE ACTIVITY IN MICROBODIES OF FETAL MOUSE LIVER

1969 ◽  
Vol 17 (7) ◽  
pp. 454-466 ◽  
Author(s):  
EDWARD ESSNER
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Peroxidase Activity ◽  
Mouse Liver ◽  
Fetal Liver ◽  
Light And Electron Microscopy ◽  
Small Population ◽  
Fetal Mouse ◽  
Fetal Mouse Liver

The peroxidase activity of microbodies in fetal mouse liver was studied by light and electron microscopy. Two types of microbodies were present; a small population of bodies that lacked a nucleoid, predominant on the 16th day of gestation, and a larger population of nucleoid-bearing microbodies, predominant on the 19th day, in association with the rough endoplasmic reticulum from which they probably originate. Both types of bodies were visualized when incubated for peroxidase activity but were negative (19th day) for acid phosphatase activity. The findings suggest that the anucleoid- and nucleoid-bearing organelles together constitute the microbody population of the fetal liver.

scholarly journals Canine Cutaneous Histiocytoma A Light and Electron Microscopic Study

1970 ◽  
Vol 7 (1) ◽  
pp. 12-27 ◽  
Author(s):  
D. F. Kelly
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Acid Phosphatase ◽  
Microscopic Study ◽  
Mononuclear Cell ◽  
Electron Microscopic Study ◽  
Light And Electron Microscopy ◽  
Perinuclear Region ◽  
Electron Microscopic

Cutaneous histiocytomas from 4 dogs were examined by light and electron microscopy. A large (up to 10 μ in diameter) mononuclear cell with prominent filiform processes of the plasma membrane predominated. Its cytoplasm contained relatively small amounts of endoplasmic reticulum and mitochondria, only occasional lysosomes, fibrils, most obvious in the perinuclear region, and small amounts of cytoplasmic debris. Acid phosphatase was not detected. Fibroblasts and collagen formed a small part of the lesion, except at the junction with surrounding dermis, where fibers were plentiful. The morphologic features of the lesion are compatible with the suggestion that the predominant cell is of histiocytic type.

scholarly journals ULTRASTRUCTURAL AND CYTOCHEMICAL OBSERVATIONS ON B-16 AND HARDING-PASSEY MOUSE MELANOMAS THE ORIGIN OF PREMELANOSOMES AND COMPOUND MELANOSOMES

1968 ◽  
Vol 16 (5) ◽  
pp. 299-319 ◽  
Author(s):  
ALEX B. NOVIKOFF ◽  
ARLINE ALBALA ◽  
LUIS BIEMPICA
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Light Microscopy ◽  
Lysosomal Enzyme ◽  
Smooth Endoplasmic Reticulum ◽  
Aryl Sulfatase ◽  
Thiamine Pyrophosphatase ◽  
Inosine Diphosphatase

The B-16 and Harding-Passey mouse melanomas were studied by light microscopy (tyrosinase, acid phosphatase, aryl sulfatase, thiamine pyrophosphatase and inosine diphosphatase activities) and electron microscopy (morphology and tyrosinase and acid phosphatase activities). Lysosomal enzyme activity is present in individual premelanosomes and melanosomes as well as in compound melanosomes. Acid phosphatase and tyrosinase activities are present in a Golgi-associated system of smooth endoplasmic reticulum (GERL) and small vesicles related to it. The acid phosphatase and tyrosinase activities of premelanosomes, and morphologic appearances, support the hypothesis that the granules arise from GERL. On the basis of the evidence presented, it is suggested that compound melanosomes arise within melanoma cells by autophagy.

Endocytosis and the lysosomal apparatus of rat Leydig cells

1984 ◽  
Vol 42 ◽  
pp. 708-709
Author(s):  
M.F. Lalli ◽  
L. Hermo ◽  
Y. Clermont
Keyword(s):  
Endoplasmic Reticulum ◽  
Plasma Membrane ◽  
Acid Phosphatase ◽  
Cell Surface ◽  
Leydig Cells ◽  
Smooth Endoplasmic Reticulum ◽  
Extracellular Fluid ◽  
Multivesicular Bodies ◽  
Rat Testis ◽  
Endocytic Activity

The Leydig cells of the rat testis which are involved in testosterone production contain an abundance of smooth endoplasmic reticulum and mitochondria (Figs. 2,6). These cells also possess many peroxisomes, lysosomes and multivesicular bodies (MVB's). On the cell surface, the plasma membrane contains numerous short microvilli, small invaginations and large plasmalemmal folds which appear to engulf extracellular fluid. There are also many large dilated vacuoles adjacent to the cell surface. The purpose of the present study is to determine if these cells show endocytic activity and to differentiate by various cytochemical means lysosomal elements from peroxisomes.To identify lysosomes, tissue chopper sections of 2% glutaraldehyde-fixed testes (containing 2.5% dextran) were incubated in media containing thiamine monophosphate as a substrate (Lalli, 1983) to demonstrate the presence of acid phosphatase or in media containing P-nitrocatechol sulfate for the demonstration of arylsulfatase (Hopsu-Havu et al., 1967).

scholarly journals LYSOSOMES IN THE RAT SCIATIC NERVE FOLLOWING CRUSH

1965 ◽  
Vol 27 (3) ◽  
pp. 651-669 ◽  
Author(s):  
Eric Holtzman ◽  
Alex B. Novikoff
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Schwann Cells ◽  
Peripheral Nerves ◽  
Wallerian Degeneration ◽  
Dense Body ◽  
Multivesicular Bodies ◽  
Body Type ◽  
Autophagic Vacuoles ◽  
Myelinated Fibers

Peripheral nerves undergoing degeneration are favorable material for studying the types, origins, and functions of lysosomes. The following lysosomes are described: (a) Autophagic vacuoles in altered Schwann cells. Within these vacuoles the myelin and much of the axoplasm which it encloses in the normal nerve are degraded (Wallerian degeneration). The delimiting membranes of the vacuoles apparently form from myelin lamellae. Considered as possible sources of their acid phosphatase are Golgi vesicles (primary lysosomes), lysosomes of the dense body type, and the endoplasmic reticulum which lies close to the vacuoles. (b) Membranous bodies that accumulate focally in myelinated fibers in a zone extending 2 to 3 mm distal to the crush. These appear to arise from the endoplasmic reticulum in which demonstrable acid phosphatase activity increases markedly within 2 hours after the nerve is crushed. (c) Autophagic vacuoles in the axoplasm of fibers proximal to the crush. The breakdown of organelles within these vacuoles may have significance for the reorganization of the axoplasm preparatory to regeneration. (d) Phagocytic vacuoles of altered Schwann cells. As myelin degeneration begins, some axoplasm is exposed. This is apparently engulfed by the filopodia of the Schwann cells, and degraded within the phagocytic vacuoles thus formed. (e) Multivesicular bodies in the axoplasm of myelinated fibers. These are generally seen near the nodes of Ranvier.

Encystment and germination of the parasitic chytrid Rozella allomycis on host hyphae

1973 ◽  
Vol 51 (10) ◽  
pp. 1825-1835 ◽  
Author(s):  
Abraham A. Held
Keyword(s):  
Cyst Wall ◽  
Germ Tube ◽  
Light And Electron Microscopy ◽  
Multivesicular Bodies ◽  
Cell Periphery ◽  
General Sequence ◽  
Obligate Intracellular ◽  
Host Cytoplasm ◽  
Intracellular Parasites ◽  
Three Stages

Zoospores of the obligately parasitic chytrid Rozella allomycis which settle upon hyphae of the water mold host, Allomyces arbuscula, encyst and germinate before their protoplasts penetrate into the host cytoplasm. This process has been examined by light and electron microscopy. Three stages which follow the attachment to the host and the retraction of the zoospore's flagellum are described: (1) the early cyst lacks a wall; it is discoid, and its shape is maintained by the coil of the retracted axoneme which forms its rim; (2) a cyst wall is formed while multivesicular bodies occur at the cell periphery and eventually disappear; a germ tube starts to grow at the point of attachment; and (3) the firm-walled cyst is spheroidal; it has a fully developed germ tube with a specialized class of vesicles; it also forms a distal, flattened vacuole whose swelling eventually injects the Rozella protoplast into the host; at this stage the retracted axoneme has disappeared and the cell's organelles have undergone extensive changes. Electron-dense, "gamma-like" granules enclosed in vacuoles may play a major role in the formation of both the cyst wall and the distal vacuole. These granules appear to give rise to small vesicles, and thus to multivesicular bodies; the distal vacuole appears to form by coalescense of gamma-like vacuoles.The general sequence of encystment and germination resembles that found in other Chytridiomycetes, both saprophytic and parasitic. However, the distal vacuole and the vesicles in the germ tube appear to be parasitic adaptations and are shared by obligate intracellular parasites from several unrelated groups of zoosporic fungi.

scholarly journals STUDIES ON MICROPEROXISOMES II. A CYTOCHEMICAL METHOD FOR LIGHT AND ELECTRON MICROSCOPY

1972 ◽  
Vol 20 (12) ◽  
pp. 1006-1023 ◽  
Author(s):  
ALEX B. NOVIKOFF ◽  
PHYLLIS M. NOVIKOFF ◽  
CLEVELAND DAVIS ◽  
NELSON QUINTANA
Keyword(s):  
Endoplasmic Reticulum ◽  
Guinea Pig ◽  
Lipid Droplets ◽  
Smooth Endoplasmic Reticulum ◽  
Light And Electron Microscopy ◽  
Distal Tubule ◽  
Striking Difference ◽  
High Concentration ◽  
Electron Microscopic ◽  
Pig Kidney

A modification of the Novikoff-Goldfischer alkaline 3,3'-diaminobenzidine medium for visualizing peroxisomes is described. It makes possible light microscopic as well as electron microscopic studies of a recently described class of peroxisomes, the microperoxisomes. Potassium cyanide (5 x 10–3 M) is included in the medium to inhibit mitochondrial staining, the pH is 9.7 and there is a high concentration of H2O2 (0.05%). Two cell types have been chosen to illustrate the advantages of the new procedure for demonstrating the microperoxisomes: the absorptive cells in the human jejunum and the distal tubule cells in the guinea pig kidney. Suggestive relations of microperoxisomes and lipid are described in the human jejunum. The microperoxisomes are strategically located between smooth endoplasmic reticulum that radiates toward the organelles and contains lipid droplets and "central domains" of highly specialized endoplasmic reticulum which do not show the lipid droplets. The microperoxisomes are also present at the periphery of large lipid-like drops. In the guinea pig kidney tubule there is a striking difference between the thick limb of Henle and distal tubule. The distal tubule has a population of cells with large numbers of microperoxisomes readily visible by light microscopy; these cells are not present in the thick limb of Henle. Other differences between the two are also described.

scholarly journals Peroxisomes in pulmonate gastropods.

1977 ◽  
Vol 25 (5) ◽  
pp. 319-328 ◽  
Author(s):  
E Dannen ◽  
M E Beard
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Positive Reaction ◽  
Smooth Endoplasmic Reticulum ◽  
Positive Staining ◽  
Staining Reaction ◽  
Arion Ater ◽  
Morphologic Characteristics ◽  
Granular Matrix

Organelles with the morphologic characteristics of peroxisomes have been found in the cells of the kidney sac of two terrestrial pulmonate gastropods. Arion ater and Ariolimax columbianus. These peroxisomes appear in profile as circles or ellipses, 0.25 micron in diameter and 0.3-0.8 micron long; They have a finely granular matrix and a single-limiting membrane; the organelles are extensively associated with smooth endoplasmic reticulum. Some Ariolimax peroxisomes contained structures reminiscent of nucleoids while those of Arion did not. The peroxisomes of Arion ater show a strongly-positive staining reaction with the 3,3'-diaminobenzidine technique, which is inhibited in the presence of aminotriazole. Peroxisomes of Ariolimax columbianus did not show a positive reaction, despite a number of variations of the 3,3'-diaminobenzidine protocol. Speculations are made concerning the biochemical reasons for this cytochemical behavior. Peroxisomes in both tissues were negatively stained while lysosomes were positively stained in acid-phosphatase incubations.

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