scholarly journals Regulation of acid phosphatase activity in human promyelocytic leukemic cells induced to differentiate in culture.

1979 ◽  
Vol 83 (2) ◽  
pp. 300-307 ◽  
Author(s):  
A Vorbrodt ◽  
P Meo ◽  
G Rovera
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Smooth Endoplasmic Reticulum ◽  
Leukemic Cell ◽  
Time Sequence ◽  
Leukemic Cells ◽  
Leukemic Cell Line ◽  
Faint Band ◽  
Induction Of Differentiation ◽  
Sequence Study

Induction of differentiation of a human promyelocytic leukemic cell line (HL60) in culture is accompanied by changes in acid phosphatase (Acpase) activity. The increase in activity is less than twofold when the leukemic cells are stimulated by dimethylsulfoxide (DMSO) to differentiate into metamyelocytes and granulocytes but is eightfold when the cells are stimulated by the tumor-promoting agent 12-0-tetradecanoylphorbol 13-acetate (TPA) to differentiate into macrophage-like cells. Five different isozymes of Acpase were separated by acrylamide gel electrophoresis. Isozyme 1, the most anodal isozyme, was found to be present in undifferentiated, DMSO-treated and TPA-treated cells; isozyme 2 was a very faint band observed both in DMSO- and TPA-treated cells, the isoenzymes 3a and 3b were present only in TPA-induced cells; and isozyme 4, the most cathodal isozyme, was present both in TPA- and DMSO-induced cells. A time sequence study on the appearance of the various forms after TPA treatment indicated that the expression of the isozymes is regulated in an uncoordinated fashion. Acpase activity has been shown by ultrastructural cytochemistry to be localized in the entire rough endoplasmic reticulum (RER) and in areas of the smooth endoplasmic reticulum (SER) located near the Golgi complex in differentiating cells but to be extremely weak, if at all detectable, in undifferentiated promyelocytes.

scholarly journals Peroxisomes in pulmonate gastropods.

1977 ◽  
Vol 25 (5) ◽  
pp. 319-328 ◽  
Author(s):  
E Dannen ◽  
M E Beard
Keyword(s):  
Endoplasmic Reticulum ◽  
Acid Phosphatase ◽  
Positive Reaction ◽  
Smooth Endoplasmic Reticulum ◽  
Positive Staining ◽  
Staining Reaction ◽  
Arion Ater ◽  
Morphologic Characteristics ◽  
Granular Matrix

Organelles with the morphologic characteristics of peroxisomes have been found in the cells of the kidney sac of two terrestrial pulmonate gastropods. Arion ater and Ariolimax columbianus. These peroxisomes appear in profile as circles or ellipses, 0.25 micron in diameter and 0.3-0.8 micron long; They have a finely granular matrix and a single-limiting membrane; the organelles are extensively associated with smooth endoplasmic reticulum. Some Ariolimax peroxisomes contained structures reminiscent of nucleoids while those of Arion did not. The peroxisomes of Arion ater show a strongly-positive staining reaction with the 3,3'-diaminobenzidine technique, which is inhibited in the presence of aminotriazole. Peroxisomes of Ariolimax columbianus did not show a positive reaction, despite a number of variations of the 3,3'-diaminobenzidine protocol. Speculations are made concerning the biochemical reasons for this cytochemical behavior. Peroxisomes in both tissues were negatively stained while lysosomes were positively stained in acid-phosphatase incubations.

scholarly journals Fluorouracil Selectively Enriches Stem-like Leukemic Cells in a Leukemic Cell Line

2010 ◽  
pp. 419-427 ◽  
Author(s):  
Ling Zhang ◽  
Song Yang ◽  
Yu-Juan He ◽  
Hui-Yuan Shao ◽  
Li Wang ◽  
...  
Keyword(s):  
Cell Line ◽  
Leukemic Cell ◽  
Leukemic Cells ◽  
Leukemic Cell Line

Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member B (ANP32B), a Novel Caspase-3 Substrate, Exerts Anti-Apoptotic Effects on Acute Myeloid Leukemic Cells.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 1337-1337
Author(s):  
Yun Yu ◽  
Shao-Ming Shen ◽  
Li-Shun Wang ◽  
Qian Zhao ◽  
Guo-Qiang Chen
Keyword(s):  
Cell Line ◽  
Nuclear Export ◽  
Caspase 3 ◽  
Expression Profiles ◽  
Leukemic Cell ◽  
Site Directed Mutagenesis ◽  
Leukemic Cells ◽  
Leukemic Cell Line ◽  
Nuclear Phosphoprotein ◽  
Acute Myeloid

Abstract The acidic leucine-rich nuclear phosphoprotein 32B (ANP32B, also called APRIL) is a member of a conserved superfamily of nuclear proteins that includes ANP32A/pp32, a factor that binds histones and inhibits their acetylation and regulates cell growth and differentiation in a tissue-specific manner. Recently, ANP32B was identified as a novel histone chaperone, and it can interact with the transcription factor KLF5, leading to transcriptional repression of a KLF5-downstream gene through stimulation of promoter region-specific histone incorporation and inhibition of histone acetylation. Additionally, ANP32B and/or ANP32A also serve as adaptor molecules linking the HuR nucleocytoplasmic shuttle protein and the nuclear export receptor CRM1 to regulate the cytoplasmic accumulation of some transcripts such as c-fos and CD83. However, its biological activity is still poorly understood. By the two-dimensional electrophoresis plus MALDI-TOF/TOF tandem mass spectrometry-based analysis of subcellular protein expression profiles, we identified ANP32B protein to become a small fragment in the cytosols of apoptotic leukemic cell line induced by NSC606985, a camptothecin analog. The ongoing immunoblot analyses confirmed that ANP32B protein was cleaved during cellular context-independent and caspase-3 activation-dependent apoptosis induced by etoposide, doxorubin and arsenic trioxide besides NSC606985. Further in vitro proteolytic experiments supported that ANP32B is a direct substrate of caspase-3, and the site-directed mutagenesis analysis identified the unclassical aspartate (AEVD163) of ANP32B sequence to be the caspase-3 targeted sites. Thus, we investigated the potential role of ANP32B in apoptosis induction. Our results showed that the suppression of ANP32B expression by siRNA in acute myeloid leukemic cell line U937 cells strongly enhances NSC606985 and etoposide-induced apoptosis. Based on these findings, this work also analyzed molecular mechanism of anti-apoptotic effect of ANP32B, and some interesting findings were confirmed.

Study of the Activity of Differentiation-Induction in a Chinese Herb-Viscum alniformosanae Part 1: In vitro induction of differentiation in HL-60 leukemic cell line by conditioned medium secreted from viscum alniformosanae-stimulated mononuclear cells

1991 ◽  
Vol 19 (01) ◽  
pp. 33-39 ◽  
Author(s):  
Ling-ling Yang ◽  
Kuo-I Hsiao ◽  
Jenn-long Su ◽  
Jacqueline Liu ◽  
Po-min Chen
Keyword(s):  
Cell Line ◽  
Conditioned Medium ◽  
Mononuclear Cells ◽  
Chinese Herb ◽  
Leukemic Cell ◽  
Leukemic Cell Line ◽  
Differentiation Induction ◽  
Induction Of Differentiation ◽  
In Vitro Induction

A conditioned medium(CM), designated as 572-CMF-, was a Chinese herb viscum alniformosanae (V.A.) stimulated mononuclear cells. This CM has the capacity to induce the promyelocytic cell line HL-60 to differentiate into morphologically and functionally mature monocytoid cells. However, our results on the effect of a combination of 572 conditioned medium and IFN-r, TNF and IL-2 were neither synergistic nor additive. Further investigation of the nature of this conditioned medium remains to be performed.

scholarly journals Constitutive expression and role in growth regulation of interleukin-1 and multiple cytokine receptors in a biphenotypic leukemic cell line

Blood ◽  
1991 ◽  
Vol 78 (1) ◽  
pp. 94-102 ◽  
Author(s):  
A Cohen ◽  
T Grunberger ◽  
W Vanek ◽  
ID Dube ◽  
PJ Doherty ◽  
...  
Keyword(s):  
Cell Line ◽  
Growth Regulation ◽  
Interleukin 1 ◽  
Constitutive Expression ◽  
Leukemic Cell ◽  
Leukemic Cells ◽  
Cytokine Receptors ◽  
Low Density ◽  
Leukemic Cell Line ◽  
B1 Cells

A cell line (B1) was established from the bone marrow of a patient with a relapse of acute leukemia characterized by a 4;11 chromosomal translocation and biphenotypic features of early B and myeloid lineages. Analysis of the growth requirements of this cell line showed density-dependent growth and secretion of an autostimulatory growth factor, suggesting an autocrine mechanism. Several lines of evidence implicate the participation of interleukin-1 (IL-1) in the autocrine growth regulation of B1 cells. These cells constitutively express the messenger RNA (mRNA) for IL-1 and IL-1 receptor and secrete IL-1; recombinant IL-1 stimulated the growth of colonies when cells were seeded at low density, and anti-IL-1 antibodies inhibited the growth of colonies with cells seeded at higher density. B1 cells do not express detectable levels of mRNA for any of the other cytokines tested, and other cytokines failed to support the growth of B1 cells at low density. In addition, B1 cells express multiple cytokine receptor genes, including the receptors for IL-6, IL-7, tumor necrosis factor and gamma-interferon. Addition of the respective cytokines to the B1 cells resulted in inhibition of the growth of leukemic cells in vitro. The multiplicity of growth-inhibitory cytokine receptors on this leukemic cell line might be due to its biphenotypic lineage and may suggest new therapeutic possibilities in controlling leukemic cell proliferation.

scholarly journals Alkaloid-rich fraction of Himatanthus lancifolius contains anti-tumor agents against leukemic cells

2010 ◽  
Vol 46 (2) ◽  
pp. 273-280 ◽  
Author(s):  
Melissa Pires de Lima ◽  
Luciana Farhat Hilst ◽  
Fernanda Vanessa Rechinbach Mattana ◽  
Cid Aimbiré de Moraes Santos ◽  
Almeriane Maria Weffort-Santos
Keyword(s):  
Annexin V ◽  
Leukemic Cell ◽  
Leukemic Cells ◽  
Main Compound ◽  
Leukemic Cell Lines ◽  
Flow Cytometric ◽  
Induction Of Differentiation ◽  
Daudi Cells ◽  
Reh Cells ◽  
Marrow Cells

The effects of the alkaloid-rich fraction of Himatanthus lancifolius (Müll. Arg) Woodson on normal marrow cells and leukemic cell lines were investigated. After 48 h exposure, the proliferation assay showed significant cell growth inhibition for Daudi (0.1-10 µg/mL), K-562 (1-10 µg/mL), and REH cells (10-100 µg/mL), yet was inert for normal marrow cells. A similar inhibition profile was observed in clonogenic assays. This alkaloid-rich fraction, in which uleine is the main compound, showed no signs of toxicity to any cells up to 10 µg/mL. Cell feature analyses after induction of differentiation showed maintenance of the initial phenotype. Flow cytometric expression of Annexin-V and 7-AAD in K-562 and Daudi cells has indicated that the cells were not undergoing apoptosis or necrosis, suggesting cytostatic activity for tumor cells

scholarly journals LacZ staining in paraffin-embedded tissue sections.

1996 ◽  
Vol 44 (11) ◽  
pp. 1323-1329 ◽  
Author(s):  
P J Hendrikx ◽  
J Vermeulen ◽  
A Hagenbeek ◽  
M Vermey ◽  
A C Martens
Keyword(s):  
Cell Line ◽  
Leukemic Cell ◽  
Promyelocytic Leukemia ◽  
Leukemic Cells ◽  
Leukemic Cell Line ◽  
Paraffin Sections ◽  
Tissue Sections ◽  
Lacz Staining ◽  
Catalyzed Reporter Deposition ◽  
First Time

Femora and tibiae of rats carrying leukemia from a LacZ-marked acute promyelocytic leukemia-derived leukemic cell line (LT12NL15) were decalcified using EDTA and routinely embedded in paraffin. Sections were used to develop for the first time an immunostaining method for LacZ, employing catalyzed reporter deposition (CARD) based on the deposition of biotinylated tyramine. This method was used to study homing and adhesion of leukemic cells.

Delta- and Gamma-Tocotrienols Induce Classical Ultrastructural Apoptotic Changes in Human T Lymphoblastic Leukemic Cells

2012 ◽  
Vol 18 (3) ◽  
pp. 462-469 ◽  
Author(s):  
Rebecca S.Y. Wong ◽  
Ammu K. Radhakrishnan ◽  
Tengku Azmi Tengku Ibrahim ◽  
Soon-Keng Cheong
Keyword(s):  
Apoptotic Cell ◽  
Chromatin Condensation ◽  
Leukemic Cell ◽  
Leukemic Cells ◽  
Flow Cytometry Analysis ◽  
Nuclear Fragmentation ◽  
Leukemic Cell Line ◽  
Apoptotic Pathway ◽  
Cytotoxic Effects ◽  
Transmission Electron

AbstractTocotrienols are isomers of the vitamin E family, which have been reported to exert cytotoxic effects in various cancer cells. Although there have been some reports on the effects of tocotrienols in leukemic cells, ultrastructural evidence of tocotrienol-induced apoptotic cell death in leukemic cells is lacking. The present study investigated the effects of three isomers of tocotrienols (alpha, delta, and gamma) on a human T lymphoblastic leukemic cell line (CEM-SS). Cell viability assays showed that all three isomers had cytotoxic effects (p < 0.05) on CEM-SS cells with delta-tocotrienol being the most potent. Transmission electron microscopy showed that the cytotoxic effects by delta- and gamma-tocotrienols were through the induction of an apoptotic pathway as demonstrated by the classical ultrastructural apoptotic changes characterized by peripheral nuclear chromatin condensation and nuclear fragmentation. These findings were confirmed biochemically by the demonstration of phosphatidylserine externalization via flow cytometry analysis. This is the first study showing classical ultrastructural apoptotic changes induced by delta- and gamma-tocotrienols in human T lymphoblastic leukemic cells.

Sign in / Sign up

Export Citation Format

Share Document