scholarly journals THE EFFECT OF ANTIBODY ON INTRACELLULAR PARASITISM OF SALMONELLA TYPHIMURIUM IN MONONUCLEAR PHAGOCYTES IN VITRO

1959 ◽  
Vol 110 (5) ◽  
pp. 715-730 ◽  
Author(s):  
Justus Gelzer ◽  
Emanuel Suter
Keyword(s):  
Salmonella Typhimurium ◽  
Gamma Globulin ◽  
Fluorescent Antibody ◽  
Immune Serum ◽  
Mononuclear Phagocytes ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Long Time ◽  
Infected Cells

The effect of antibody on the fate of Salmonella typhimurium within mononuclear phagocytes (MN) of rabbits was studied in vitro. Monocytes and bacteria were incubated either in absence or presence of antibody. After 45 minutes during which phagocytosis occurred infected cells were washed to remove extracellular bacilli and free antibody. The cells were then reincubated in a medium without addition of antibody, and the interaction between the MN and bacteria was followed, correlating bacterial viability and the morphology of the mixture. The following results were obtained. The anti-Salmonella antibody was not bactericidal even in presence of complement and did not enhance phagocytosis. Regardless of whether antibody was present or absent during phagocytosis, the bacteria appeared to multiply within the cells. When no antibody was present during phagocytosis the infected cells were severely damaged within a few hours of incubation, and extensive extracellular multiplication was dominating. When antibody was present during phagocytosis MN packed with bacteria persisted for a long time. Little or no extracellular growth occurred. It was possible to demonstrate the presence of the antibody within the infected MN, using the fluorescent antibody technique. The antibody appeared as a coat around the bacteria. Antibody entered the cells only during phagocytosis, presumably attached to the bacteria. The active factor of the immune serum was found in the gamma globulin fraction and reacted specifically with the somatic antigen of Salmonella typhimurium. The antiflagellar portion of the antiserum was not involved in the phenomenon described. It is concluded that this antibody protects monocytes against the effect of intracellularly located Salmonella.

scholarly journals CELLULAR SITES OF FORMATION OF GAMMA GLOBULIN

1957 ◽  
Vol 106 (5) ◽  
pp. 627-640 ◽  
Author(s):  
L. G. Ortega ◽  
R. C. Mellors
Keyword(s):  
Germinal Center ◽  
Gamma Globulin ◽  
Plasma Cells ◽  
Serum Proteins ◽  
Fluorescent Antibody ◽  
Germinal Centers ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Reticular Cells ◽  
Parenchymal Cells

The cellular sites of formation of γglobulin in lymphatic tissues of man and in a representative human lymphoid infiltrate have been studied by fluorescent antibody technique. The findings indicate that γ-globulin is formed in the germinal centers of lymphatic nodules and in the cytoplasm of mature and immature plasma cells of two types—those with and those without Russell bodies. The germinal center cells that synthesize γ-globulin have been designated "intrinsic" cells to distinguish them from the medium and large lymphocytes, and the primitive reticular cells that occur elsewhere and do not produce γ-globulin. Unlike the plasma cells, which function as individual units, the intrinsic cells apparently form γ-globulin only when they are arranged in discrete aggregations. The function, the blood supply, and the systematic cellular arrangement of germinal centers justifies the postulate that they are miniature organs of internal secretion of γ-globulin. The release of γ-globulin from its sites of formation appears to be accomplished by holocrine and apocrine secretion. Presumably, these secretory mechanisms are adaptations required for the production of antibody since they have not been described in parenchymal cells that form the other serum proteins. The cells found to form γ-globulin appear to be identical with those previously shown to form specific antibody in response to a variety of antigens in the experimental animal. This evidence indicates that normal γ-globulin, if it exists, originates in the same cells that produce antibody. It is suggested, also, that each of the 3 morphologically distinct categories of cells that synthesize γ-globulin represents a response to a particular form of antigenic stimulation. Nuclear participation in the process of γ-globulin synthesis was not detected by the technique employed.

scholarly journals In vitro methods for rabies virus detection, and evaluation of their use in the production of rabies immunoglobulin

2021 ◽  
Vol 21 (2) ◽  
pp. 76-84
Author(s):  
Yu. K. Gavrilova ◽  
S. V. Generalov ◽  
E. G. Abramova ◽  
A. K. Nikiforov
Keyword(s):  
Rabies Virus ◽  
Fluorescent Antibody ◽  
Enzyme Linked Immunosorbent Assay ◽  
Fluorescent Antibody Technique ◽  
Biological Method ◽  
Culture Methods ◽  
Antibody Technique ◽  
Highly Sensitive ◽  
Antibodies Detection

Current highly sensitive methods for rabies virus and rabies antibodies detection in biological material can be used not only for diagnosis and experimental research, but also for the production of antirabies medicines used for postexposure prophylaxis. The aim of the study was to analyse existing methods for rabies virus and rabies antibodies detection and to assess the potential for using these methods at the control stages during production of heterologous antirabies immunoglobulin obtained from equine serum. The search for cutting-edge highly sensitive in vitro control methods that could compete with the biological method, which is the main method used in antirabies immunoglobulin control, is an important prerequisite for improvement of the production technology and the quality of antirabies medicines. The study demonstrated that the following test methods can be used in the production of antirabies immunoglobulin: fluorescent antibody technique, enzyme-linked immunosorbent assay, cell culture methods, atomic force microscopy, and flow cytometry. These methods could be used alone or as an alternative to the biological method in white mice. These methods were chosen because of their high sensitivity, specificity, rapid and easy implementation, cost-effectiveness, and automatic recording of test results.

STUDIES ON THE IMMUNOLOGICAL SPECIFICITY OF AUTOLOGOUS GAMMA-GLOBULIN IN THE GLOMERULI OF RABBITS WITH EXPERIMENTALLY INDUCED GLOMERULONEPHRITIS

1963 ◽  
Vol 41 (4) ◽  
pp. 897-904
Author(s):  
A. L. Sherwin ◽  
A. Leznoff ◽  
M. Richter ◽  
B. Rose
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Specific Antibody ◽  
Bovine Serum ◽  
Gamma Globulin ◽  
High Titer ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Immunological Specificity

Experimental glomerulonephritis was induced in rabbits by the intravenous administration of chicken antirabbit glomerulus serum (nephrotoxic serum). These rabbits were simultaneously immunized, either actively or passively, with bovine serum albumin or ovalbumin. Autologous gamma-globulin was identified in the glomeruli of these nephritic rabbits by means of the fluorescent antibody technique. It was not possible to demonstrate the presence of an anti-BSA or anti-OA component in the gamma-globulin even though these antibodies were present in high titer in the blood. This suggests that the autologous gamma-globulin present in the lesions is entirely specific antibody rather than an accumulation of serum gamma-globulin.

STUDIES ON THE IMMUNOLOGICAL SPECIFICITY OF AUTOLOGOUS GAMMA-GLOBULIN IN THE GLOMERULI OF RABBITS WITH EXPERIMENTALLY INDUCED GLOMERULONEPHRITIS

1963 ◽  
Vol 41 (1) ◽  
pp. 897-904
Author(s):  
A. L. Sherwin ◽  
A. Leznoff ◽  
M. Richter ◽  
B. Rose
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Specific Antibody ◽  
Bovine Serum ◽  
Gamma Globulin ◽  
High Titer ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
Immunological Specificity

Experimental glomerulonephritis was induced in rabbits by the intravenous administration of chicken antirabbit glomerulus serum (nephrotoxic serum). These rabbits were simultaneously immunized, either actively or passively, with bovine serum albumin or ovalbumin. Autologous gamma-globulin was identified in the glomeruli of these nephritic rabbits by means of the fluorescent antibody technique. It was not possible to demonstrate the presence of an anti-BSA or anti-OA component in the gamma-globulin even though these antibodies were present in high titer in the blood. This suggests that the autologous gamma-globulin present in the lesions is entirely specific antibody rather than an accumulation of serum gamma-globulin.

scholarly journals Fluorescent Antibody Technique For Detection Of Salmonella Typhimurium In The Intestine Of Experimentally Infected Calves

2008 ◽  
Vol 32 (2) ◽  
pp. 290-298
Author(s):  
Faisal G. Habasha
Keyword(s):  
Salmonella Typhimurium ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Indirect Fluorescent Antibody ◽  
Antibody Technique ◽  
Reliable Technique ◽  
Group I ◽  
Group Ii

A systemic study on the pathogenesis of experimentally inducedSalmonella typhimurium infection was carried out in calves. Sixteen normal,colostrum-fed, friesian calves, ranging in age from 3 to 6 weeks were used. Thecalves were divided into two equal groups. Group I calves were inoculatedorally with 1.5 x 1011 CFU of Salmonella typhimurium and group II calvesserved as control.Using an indirect fluorescent antibody technique, it was found thatSalmonella typhimurium has an apparent predilection for the small intestineparticularly jejunum and ileum. The results of this study suggest that thistechnique can be considered as a rapid reliable technique for diagnosis ofsalmonellosis in endemic outbreaks.

Die Bedeutung der Plasmazellen als Antikörperbildner

1964 ◽  
Vol 1 (5) ◽  
pp. 423-453 ◽  
Author(s):  
G. Trautwein
Keyword(s):  
Blood Serum ◽  
Antibody Formation ◽  
Plasma Cells ◽  
Fluorescent Antibody ◽  
Experimental Investigations ◽  
Antibody Concentration ◽  
Fluorescent Antibody Technique ◽  
Antibody Technique ◽  
And Function

During the last two decades, it has been shown by a great number of morphological, clinical, and experimental investigations that the humoral antibodies are formed by plasma cells. Plasma cells are immobile connective tissue cells which are formed in the medullary cords of lymph nodes, the red pulp of the spleen, the bone marrow, and in the adventitia of small blood vessels. The theory of the plasmacellular antibody formation is based on these clinical and experimental observations: (1) Correlation of hypergammaglobulinemia in the blood serum and proliferation of plasma cells in the tissue in various diseases of men and animals. (2) Correlation of antibody concentration in the blood serum and plasma cell proliferation in hyperimmunized experimental animals. (3) Demonstration of antibody formation in plasma cells by in vitro experiments and extraction of γ-globulin from plasma cells. (4) Detection of antibodies in plasma cells with the fluorescent antibody technique. Macrophages and lymphocytos also play a role in antibody formation. While it is the function of the macrophages to transform corpuscular antigen into soluble immunogenic antigen, the lymphocytes play the role of a co-factor. In newborns the thymus lymphocytes transmit substances which are necessary for the development and function of the antibody producing system. The complicated problems of globulin synthesis in the antibody producing cell are explained in the light of the genetic theory of antibody formation advanced by Ehrich.

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