scholarly journals IMMUNE MECHANISMS IN PARABIOSIS INTOXICATION

1964 ◽  
Vol 119 (4) ◽  
pp. 567-579 ◽  
Author(s):  
Henry R. Hilgard ◽  
Eugene A. Cornelius ◽  
Agustin P. Dalmasso ◽  
Carlos Martinez ◽  
Robert A. Good
Keyword(s):  
Parental Strain ◽  
Immunological Tolerance ◽  
The Other ◽  
Term Survival ◽  
F1 Hybrid ◽  
Immunological Mechanism ◽  
Adult Mice ◽  
Hybrid Mice ◽  
Normal Hybrid

When A strain mice are placed in parabiotic union with (A x C57Bl/1)F1 hybrid partners, the parental strain partners are polycythemic and the hybrids anemic from the 5th through the 16th parabiosis days. All hybrids develop clinical intoxication between the 7th and the 12th days, and no pairs survive to 1 month. Long-term survival of parabiotic pairs can be achieved if lethally irradiated or specifically tolerant parental strain mice are united to hybrid partners. Production of tolerance by either of these methods results in elimination of anemia-polycythemia by the 12th parabiosis day and prevents intoxication in the hybrid partners. Preimmunization of the parental strain partners against the C57Bl/1 component of the hybrid leads to a considerable intensification of day 5 anemia-polycythemia. Intoxication develops in the hybrid partners between the 4th and the 6th days after union. It is concluded that anemia is primarily responsible for the syndrome of clinical intoxication. Early anemia-polycythemia on day 5 does not depend upon an immunological mechanism, but the late anemia-polycythemia appearing between days 12 and 16 is a function of the ability of the parental strain mouse to react immunologically against its hybrid partner. When neonatally thymectomized A strain mice are joined to hybrid partners, anemia-polycythemia is sustained through the 16th day and the hybrid partners develop clinical intoxication. On the other hand, when both partners are neonatally thymectomized, late anemia-polycythemia is considerably reduced, and the hybrid partners apparently do not develop clinical intoxication. It is concluded that normal hybrid mice are capable of reconstituting the immunological capacity of their thymectomized partners, whereas thymectomized hybrid mice do not have this restorative capacity. These findings are discussed in terms of their possible application to the problem of the induction of immunological tolerance in adult mice by the parabiosis procedure.

scholarly journals Tolerance and pancreatic islet transplantation

2001 ◽  
Vol 356 (1409) ◽  
pp. 759-765 ◽  
Author(s):  
Luca Inverardi ◽  
Camillo Ricordi
Keyword(s):  
Diabetes Mellitus ◽  
Islet Transplantation ◽  
Type I Diabetes ◽  
Immunological Tolerance ◽  
Type I Diabetes Mellitus ◽  
Current Status ◽  
Type I ◽  
Pancreatic Islet Transplantation ◽  
Term Survival

Islet transplantation holds renewed promise as a cure for type I diabetes mellitus. Results of recent clinical trials have shown remarkable success, and have reignited universal optimism for this procedure. In spite of this success, the need for life–long immunosuppression of the recipient still limits islet transplantation to patients with poorly controlled diabetes or to those requiring kidney transplantation. It is obvious that the achievement of immunological tolerance would broaden the indication for islet transplantation to a much larger cohort of patients with type I diabetes mellitus, most likely preventing long–term complications and contributing to a much improved quality of life. Increased understanding of the basic mechanisms of tolerance induction has resulted in the implementation of numerous experimental approaches to achieve long–term survival of islet grafts in the absence of chronic immunosuppression. In this brief review we will attempt to summarize the current status of research and knowledge.

scholarly journals Constitutive expression of bcl-2 in B cells causes a lethal form of lupuslike autoimmune disease after induction of neonatal tolerance to H-2b alloantigens.

1996 ◽  
Vol 183 (6) ◽  
pp. 2523-2531 ◽  
Author(s):  
M López-Hoyos ◽  
R Carrió ◽  
R Merino ◽  
L Buelta ◽  
S Izui ◽  
...  
Keyword(s):  
B Cells ◽  
Autoimmune Disease ◽  
Constitutive Expression ◽  
Systemic Autoimmune Disease ◽  
Spleen Cells ◽  
Term Survival ◽  
F1 Hybrid ◽  
Wide Range

The bcl-2 protooncogene has been shown to provide a survival signal to self-reactive B cells, but it fails to override their developmental arrest after encounter with antigen. Furthermore, constitutive expression of bcl-2 in B cells does not promote the development of autoimmune disease in most strains of mice, indicating that signals other than those conferred by bcl-2 are required for long-term survival and differentiation of self-reactive B cells in vivo. To further examine the factors that are required for the pathogenesis of autoimmune disease, we have assessed the effect of bcl-2 overexpression on the development of host-versus-graft disease, a self-limited model of systemic autoimmune disease. In this model, injection of spleen cells from (C57BL/6 x BALB/c)F1 hybrid mice into BALB/c newborn parental mice induces immunological tolerance to donor tissues and activation of autoreactive F1 donor B cells through interactions provided by allogeneic host CD4+ T cells. BALB/c newborns injected with spleen cells from (C57BL/6 x BALB/c)F1 mice expressing a bcl-2 transgene in B cells developed high levels of anti-single-stranded DNA and a wide range of pathogenic autoantibodies that were not or barely detectable in mice injected with nontransgenic spleen cells. In mice injected with transgenic B cells, the levels of pathogenic autoantibodies remained high during the course of the study and were associated with long-term persistence of donor B cells, development of a severe autoimmune disease, and accelerated mortality. These results demonstrate that bcl-2 can provide survival signals for the maintenance and differentiation of autoreactive B cells, and suggest that both increased B cell survival and T cell help play critical roles in the development of certain forms of systemic autoimmune disease.

scholarly journals Brief Report: Graft-Versus-Host Reaction in F1 Hybrid Mice Injected with Pre-Immunized Parental Thymus Cells

Blood ◽  
1964 ◽  
Vol 24 (6) ◽  
pp. 770-774 ◽  
Author(s):  
LUCIANO FIORE-DONATI ◽  
LUIGI CHIECO-BIANCHI ◽  
GIUSEPPE DE BENEDICTIS ◽  
GIUSEPPE TRIDENTE
Keyword(s):  
Lymphoid Cells ◽  
The Other ◽  
F1 Hybrids ◽  
Graft Versus Host Reaction ◽  
Host Reaction ◽  
F1 Hybrid ◽  
Graft Versus Host ◽  
Immunologic Activity ◽  
Thymus Cells ◽  
Hybrid Mice

Abstract Dissociated thymus cells are capable of initiating graft-versus-host reaction in (C3Hf/Gs x DBA/2)F1 hybrids only when derived from parental donors previously sensitized against the antigens of the other parental strain. The lower immunologic activity of thymus cells as compared with other lymphoid cells is presumably due to quantitative rather than qualitative differences in immunologically competent cells.

scholarly journals Flt3 Ligand–treated Neonatal Mice Have Increased Innate Immunity Against Intracellular Pathogens and Efficiently Control Virus Infections

2003 ◽  
Vol 197 (5) ◽  
pp. 575-584 ◽  
Author(s):  
Sabine Vollstedt ◽  
Marco Franchini ◽  
Hans P. Hefti ◽  
Bernhard Odermatt ◽  
Meredith O'Keeffe ◽  
...  
Keyword(s):  
B Cells ◽  
Bacterial Infections ◽  
Fold Increase ◽  
Virus Infections ◽  
Term Survival ◽  
Newborn Mice ◽  
Adult Mice ◽  
Control Virus ◽  
Simplex Virus

Flt-3 ligand (FL), a hematopoetic growth factor, increases the number of dendritic cells (DCs), B cells, and natural killer cells in adult mice but the effect in neonates was unknown. We show that FL treatment of newborn mice induced a >100-fold increase in the innate resistance against infection with herpes simplex virus type 1 and Listeria monocytogenes. This resistance required interferon (IFN)-α/β for viral and interleukin (IL)-12 for bacterial infections. Long-term survival after viral but not bacterial infection was increased ∼100-fold by FL treatment. After treatment, CD11c+/major histocompatibility complex type II+ and CD11c+/B220+ DC lineage cells were the only cell populations increased in the spleen, liver, peritoneum, and skin. DC induction was independent of IFNs, IL-2, -4, -7, -9, -15, and mature T and B cells. The data suggest that FL increases the number of DCs in neonates and possibly in other immune-compromised individuals, which in turn improves IFN-α/β– and IL-12–associated immune responses.

STRAIN DIFFERENCES IN TESTICULAR WEIGHT AND SPERMATOGENESIS WITH SPECIAL REFERENCE TO C57BL/10J AND DBA/2J MICE

1972 ◽  
Vol 55 (1) ◽  
pp. 163-171 ◽  
Author(s):  
J. G. M. SHIRE ◽  
A. BARTKE
Keyword(s):  
Strain Differences ◽  
The Other ◽  
Seminiferous Tubules ◽  
Adult Mice ◽  
Testicular Weight ◽  
The Absolute ◽  
The Difference ◽  
Hybrid Mice ◽  
Germinal Cells ◽  
Type A Spermatogonia

SUMMARY Marked differences were found in the absolute and relative weights of the testes of young adult mice from 11 strains. DBA/2J mice had large testes (936 ± 46 mg/100 g body weight) and C57BL/10J mice had small ones (389 ± 6 mg/100 g). Comparisons of mice from these two strains, raised under three different environmental conditions, showed that the difference between the strains was relatively unaffected by environmental variation. Measurements on hybrid mice confirmed that much of the observed difference between the two strains was genetic in origin. The C57BL/10 mice were unlike those of any of the other strains in that both the relative and the absolute weights of the testis declined between the ages of 9 and 16 weeks. Strain differences were also found when spermatogenesis was studied in four of the strains by counting the different types of germinal cells in seminiferous tubules in stage VII of spermatogenesis. There were about twice as many type A spermatogonia in DBA/2 mice as there were in C57BL/10 mice. The mean numbers of spermatocytes and spermatids were much greater in DBA/2 than in C57BL/10. These differences were sufficient to account for the observed differences between these strains in testicular weight. Reciprocal F1 mice resembled their DBA/2 parents both in the weight of their testes and in the pattern of spermatogenesis. It is suggested that, in comparison with mice of the other strains, C57BL/10J mice may be deficient in androgenic hormones.

Corporate culture and long-term survival of Spanish innovative firms

2017 ◽  
Vol 9 (4) ◽  
pp. 335-354 ◽  
Author(s):  
Manuel Ramón Tejeiro Koller ◽  
Patricio Morcillo Ortega ◽  
José Miguel Rodríguez Antón ◽  
Luís Rubio Andrada
Keyword(s):  
Corporate Culture ◽  
Business Environment ◽  
The Other ◽  
Management Tool ◽  
Term Survival ◽  
Content Type ◽  
Innovation Culture ◽  
Innovative Firms ◽  
Innovative Capabilities

Purpose The purpose of this paper is to analyze how firms can enhance their innovative capabilities and become more resilient. The current business environment requires a specific type of management for companies to remain competitive and innovation plays a key role in this respect. However, this means that a particular kind of corporate culture must promote innovation in the firm. This innovation culture is likely to be present in innovative companies that have survived in the long term (at least 50 years) and be the source of an adaptive advantage. Design/methodology/approach Using innovative Spanish firms, which were established at least 50 years ago, an exploratory factorial analysis was conducted to verify the existence of an innovation culture. Thereafter, a cluster analysis was undertaken to study differences in performance to be able to detect and identify their adaptive advantage. Findings The findings offer a detailed profile of old and innovative firms created in Spain. Results show that most of the studied firms (88 per cent) have an innovation culture. Furthermore, two separate groups were identified, in which one showed higher profitability and a lower adjustment to an innovation culture, while the other showed the reverse results. This suggests that innovation culture helps companies be more resilient but does not necessarily lead to higher returns. Practical implications Corporate culture is identified as a useful management tool in the search for more resilient enterprises. Specific cultural traits are recommended and a benchmarking tool is applied and made available upon request. Originality/value Although there are a number of studies which consider the concept of adaptive advantage and resilience on the one side, and on corporate innovation culture on the other, this paper seems to be the first to empirically explore the relationship of both these concepts.

Longevity ofEchinostoma caproniin Balb/c mice

2008 ◽  
Vol 82 (1) ◽  
pp. 95-96 ◽  
Author(s):  
B. Fried ◽  
R.C. Peoples
Keyword(s):  
The Other ◽  
Term Survival ◽  
Long Term Survival ◽  
Echinostoma Caproni ◽  
Host Parasite Relationship ◽  
Parasite Relationship ◽  
The Mean ◽  
Host Parasite ◽  
Relationship Of

AbstractThis study used Balb/c mice to examine the longevity ofEchinostoma caproni. Five mice each exposed to 75 encysted metacercariae (cysts) were necropsied at 23 weeks postinfection (PI) (160 days PI). Two of the 5 were infected with a total of 33 worms; 23 in one mouse and 10 in the other. Body and organ area measurements showed that these worms were robust and normal in appearance. No signs of atrophy of any of the genital structures were observed. The mean ± SE of eggs/uterus per worm (n = 10) was 243 ± 6. This strain of mouse will be suitable to study the effect of long-term survival on the host–parasite relationship ofE. caproniin Balb/c mice.

scholarly journals The TALE Homeodomain Protein Pbx2 Is Not Essential for Development and Long-Term Survival

2004 ◽  
Vol 24 (12) ◽  
pp. 5324-5331 ◽  
Author(s):  
Licia Selleri ◽  
Jorge DiMartino ◽  
Jan van Deursen ◽  
Andrea Brendolan ◽  
Mrinmoy Sanyal ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Late Gestation ◽  
Homeodomain Protein ◽  
Mammalian Development ◽  
Term Survival ◽  
Long Term Survival ◽  
Embryonic Tissues ◽  
Adult Mice ◽  
Tale Homeodomain

ABSTRACT Pbx2 is one of four mammalian genes that encode closely related TALE homeodomain proteins, which serve as DNA binding partners for a subset of Hox transcription factors. The expression and contributions of Pbx2 to mammalian development remain undefined, in contrast to the essential roles recently established for family members Pbx1 and Pbx3. Here we report that Pbx2 is widely expressed during embryonic development, particularly in neural and epithelial tissues during late gestation. Despite wide Pbx2 expression, mice homozygous mutant for Pbx2 are born at the expected Mendelian frequencies and exhibit no detectable abnormalities in development and organogenesis or reduction of long-term survival. The lack of an apparent phenotype in Pbx2− /− mice likely reflects functional redundancy, since the Pbx2 protein is present at considerably lower levels than comparable isoforms of Pbx1 and/or Pbx3 in embryonic tissues. In postnatal bone marrow and thymus, however, Pbx2 is the predominant high-molecular-weight (MW)-isoform Pbx protein detectable by immunoblotting. Nevertheless, the absence of Pbx2 has no measurable effect on steady-state hematopoiesis or immune function in adult mice, suggesting possible compensation by low-MW-isoform Pbx proteins present in these tissues. We conclude that the roles of Pbx2 in murine embryonic development, organogenesis, hematopoiesis, immune responses, and long-term survival are not essential.

scholarly journals Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. I. Demonstration of similar or identical idiotypes on IgG molecules and T-cell receptors with specificity for the same alloantigens.

1975 ◽  
Vol 142 (1) ◽  
pp. 197-211 ◽  
Author(s):  
H Binz ◽  
H Wigzell
Keyword(s):  
T Cell ◽  
T Lymphocytes ◽  
T Lymphocyte ◽  
T Cell Receptors ◽  
Parental Strain ◽  
The Other ◽  
Antigenic Determinants ◽  
Antigen Binding ◽  
F1 Hybrid ◽  
Cell Receptors

Antigen-binding receptors on T lymphocytes and IgG antibodies with the same antigen-binding specificity as the T-cell receptors display shared or identical idiotypes. This was shown using a system where adult F1 hybrid rats between two inbred strains were inoculated with T lymphocytes from one parental strain. Such F1 hybrid rats produce antibodies directed against idiotypic determinants present on IgG alloantibodies, produced in the T donor genotype strain and with specificity for the alloantigens of the other parental strain. The idiotypic nature of the F1 antialloantibody serum against the parental alloantibodies was demonstrated both by indirect hemagglutination tests or by gel diffusion using alloantisera with different specificity as targets. Furthermore, the F1 anti-T-lymphocyte sera could be shown to contain antibodies against idiotypic parental T lymphocytes as well. This was shown by the capacity of the antisera, in the presence of complement, to wipe out the relevant parental T-cell reactivity against the other parental strain (as measured in MLC or GVH) whilst leaving the T-lymphocyte reactivity against a third, unrelated allogeneic strain intact. These findings demonstrate that F1 hybrid rats inoculated with parental T lymphocytes make anti-idiotypic antibodies directed against both the T cell receptors and IgG alloantibodies of that parental strain with specificity for alloantigens of the other parental strain. In order to prove identity between the anti-idiotypic antibodies against the B and T-cell antigen-binding molecules the following experiments were carried out; highly purified IgG from relevant alloantibody-containing serum in immunosorbent from could be shown to selectively remove both anti-idiotypic activities from the F1 antiserum. Further more, parental normal T lymphocytes could be shown capable of removing from the anti-idiotypic antisera all those antibodies that would cause agglutination of the relevant alloantibody-coated erythrocytes in the indirect agglutination assay. We would thus conclude that T and B lymphocytes reactive against a given antigenic determinant use receptors with antigen-binding areas coded for by the same variable gene subset(s).

Sign in / Sign up

Export Citation Format

Share Document