scholarly journals PATHOGENESIS OF THE GLOMERULONEPHRITIS OF NZB/W MICE

1968 ◽  
Vol 127 (3) ◽  
pp. 507-522 ◽  
Author(s):  
P. H. Lambert ◽  
Frank J. Dixon
Keyword(s):  
Antibody Response ◽  
Antinuclear Antibody ◽  
Active Immunization ◽  
Rapid Progression ◽  
Nuclear Antigens ◽  
Glomerular Lesions ◽  
Dna Antibodies ◽  
Capillary Walls

The development of glomerulonephritis in NZB/W mice is closely related to the formation of antinuclear, particularly anti-DNA, antibodies. The developing inflammatory glomerular lesions are characterized by the deposition of γG- and ß1C-globulins plus DNA and possibly other nuclear antigens, presumably as complexes, in a granular to lumpy pattern along the capillary walls and in the mesangia. Elution studies revealed the γG-globulin in the glomeruli to be largely γG2A-type antibody to soluble nuclear antigens. Enhancement of the antinuclear antibody response by active immunization of young NZB/W mice with DNA-methylated BSA hastens the development and increases the severity of the glomerulonephritis. Similarly, injections of soluble DNA into NZB/W mice with circulating anti-DNA antibodies but with as yet little nephritis causes rapid progression of nephritis.

scholarly journals Glomerulonephritis in schistosomiasis with mesangial IgM deposits

1981 ◽  
Vol 76 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Ageu Godoy Magalhães Filho ◽  
Antônio Victoriano Barbosa ◽  
Teresa Cristina Ferreira
Keyword(s):  
Cell Proliferation ◽  
Light Microscopy ◽  
Immunofluorescence Microscopy ◽  
The Other ◽  
Proliferative Glomerulonephritis ◽  
Mesangial Proliferative Glomerulonephritis ◽  
Normal Pattern ◽  
Glomerular Lesions ◽  
Capillary Walls ◽  
Igm Deposits

Twenty one cases of hepatoesplenic schistosomiasis patients without clinical and laboratory evidence of renal disease, were studied by surgical biopsies using light microscopy and immunofluorescence. The cases were classified histologically as: normal pattern (6 cases); minimal changes (6 cases); and mesangial proliferative glomerulonephritis (9 cases). By the immunofluorescence microscopy using anti IgM, IgG, IgA and C3, the predominant finding in all biopsies, except the normal cases, was granular deposits of IgM in the mesangium along with C3. On the other hand, IgG was present in all cases including normal biopsies along the capillary walls. However IgG was also present in the mesangium only in cases with glomerular lesions. This finding may well be similar to that recently described as IgM mesangial nephropathy. According to our cases a mesangial proliferative glomerulonephritis, characterized by segmental cell proliferation and deposition of IgM in the mesangium, is probably the entity found in the early stages of mansonic schistosomiasis.

PATHOGENESIS OF IMMUNE COMPLEX GLOMERULONEPHRITIS OF NEW ZEALAND MICE

1971 ◽  
Vol 134 (3) ◽  
pp. 65-71 ◽  
Author(s):  
Frank J. Dixon ◽  
Michael B. A. Oldstone ◽  
Giorgio Tonietti
Keyword(s):  
New Zealand ◽  
Immune Complex ◽  
Antibody Formation ◽  
Antinuclear Antibody ◽  
Viral Infections ◽  
Antibody Responses ◽  
Viral Antigens ◽  
Chronic Viral Infections ◽  
Nuclear Antigens ◽  
Antigen Antibody

Observations based on elution of IgG from nephritic kidneys of NZ mice and absorption of the eluted IgG with selected antigens indicate that their immune complex nephritis involves at least two kinds of antigen-antibody complexes. Antibodies reactive with nuclear antigens account for nearly half of the IgG eluted from the kidneys while antibodies reactive with Gross viral antigens make up a significant but lesser amount. Superimposed chronic viral infections affect the nephritis of NZ mice in different ways. LCM and polyoma infections hasten and intensify the antinuclear antibody responses and glomerulonephritis of these mice while LDV infection appears to protect against both antinuclear antibody formation and development of nephritis.

scholarly journals Breaking Tolerance to Double Stranded DNA, Nucleosome, and Other Nuclear Antigens Is Not Required for the Pathogenesis of Lupus Glomerulonephritis

2004 ◽  
Vol 199 (2) ◽  
pp. 255-264 ◽  
Author(s):  
Samuel T. Waters ◽  
Marcia McDuffie ◽  
Harini Bagavant ◽  
Umesh S. Deshmukh ◽  
Felicia Gaskin ◽  
...  
Keyword(s):  
Lupus Erythematosus ◽  
Antinuclear Antibody ◽  
Early Mortality ◽  
Chromosome 1 ◽  
Chronic Glomerulonephritis ◽  
Chromosome 4 ◽  
Double Stranded Dna ◽  
Nuclear Antigens ◽  
Dsdna Antibody ◽  
Lupus Glomerulonephritis

In lupus-prone NZM2328 mice, a locus Cgnz1 on chromosome 1 was linked to chronic glomerulonephritis, severe proteinuria, and early mortality in females. A locus Adnz1 on chromosome 4 was linked to antinuclear antibody (ANA) and anti–double stranded DNA (dsDNA) antibody (Ab) production. In this investigation, two congenic strains, NZM2328.C57L/Jc1 (NZM.C57Lc1) and NZM2328.C57L/Jc4 (NZM.C57Lc4), were generated by replacing the respective genetic intervals containing either Cgnz1 or Adnz1 with those from C57L/J, a nonlupus-prone strain. The NZM.C57Lc1 females had markedly reduced incidence of chronic glomerulonephritis and severe proteinuria. NZM.C57Lc4 females had chronic glomerulonephritis and severe proteinuria without circulating ANA, anti-dsDNA, and antinucleosome Ab. These data confirm the linkage analysis. Unexpectedly, NZM.C57Lc1 females had little anti-dsDNA and related Ab, suggesting the presence of a second locus Adnz2 on chromosome 1. The diseased NZM.C57Lc4 kidneys had immune complexes by immunofluorescence and electron microscopy. The eluates from these kidneys did not contain ANA, anti-dsDNA, and antinucleosome Ab, indicative of the presence of non–anti-dsDNA nephritogenic Ab. Thus, breaking tolerance to dsDNA and chromatin is not required for the pathogenesis of lupus nephritis. These results reaffirm that anti-dsDNA and related Ab production and chronic glomerulonephritis are under independent genetic control. These findings have significant implications in the pathogenesis of systemic lupus erythematosus.

The Clinical Significance of the Antinuclear Antibody Test as a Screening Procedure for DNA Antibodies in SLE

1980 ◽  
Vol 25 (4) ◽  
pp. 293-298 ◽  
Author(s):  
A. E. El-Ghobarey ◽  
D. J. P. Sloane ◽  
K. Whaley
Keyword(s):  
Dna Binding ◽  
Clinical Significance ◽  
Antinuclear Antibody ◽  
Antibody Test ◽  
Diagnostic Value ◽  
Screening Procedure ◽  
Dna Antibodies ◽  
Screening Procedures

During the two-year period (1st October 1974–30th September 1976), 2979 sera were tested for DNA antibodies by the Farr test. One thousand six hundred and ninety-five of these were tested because they had high ANA titres (1/256 or greater). In this group 285 sera were found to have raised DNA binding capacities (DNA-bc), 86 of which were found in patients having diagnoses other than SLE. When the diagnoses were reviewed following the finding of a raised DNA-bc, 55 of these patients were found to be suffering from SLE. Of the 1284 sera tested for DNA antibodies without the prior ANA screening procedures, 288 were positive, 36 of which came from patients not considered to have SLE; 30 of these patients were subsequently shown to have SLE. Thus the DNA-bc test is an important tool in the diagnos is of SLE, and the ANA test appears to be a valuable screening procedure. The level of DNA-bc was not of any diagnostic value.

Progression of Nailfold Microvascular Damage and Antinuclear Antibody Pattern in Systemic Sclerosis

2013 ◽  
Vol 40 (5) ◽  
pp. 634-639 ◽  
Author(s):  
Alberto Sulli ◽  
Barbara Ruaro ◽  
Vanessa Smith ◽  
Carmen Pizzorni ◽  
Giuseppe Zampogna ◽  
...  
Keyword(s):  
Systemic Sclerosis ◽  
Median Time ◽  
Antinuclear Antibody ◽  
Antinuclear Antibodies ◽  
Rapid Progression ◽  
Slight Reduction ◽  
Nailfold Videocapillaroscopy ◽  
Microvascular Damage ◽  
Antibody Pattern ◽  
Active Disease

Objective.This study evaluates possible correlations between the pattern of antinuclear antibodies (ANA) on indirect immunofluorescence (IIF) testing and nailfold microangiopathy stage (early, active, and late stage) in systemic sclerosis (SSc). Patients with SSc were followed prospectively to monitor progression of microvascular damage.Methods.The ANA pattern on IIF was searched in 42 patients with SSc showing an early pattern of nailfold microangiopathy at baseline, and was followed using nailfold videocapillaroscopy (NVC) for a median time of 91 months.Results.Among patients whose microangiopathy showed a rapid progression from early to late pattern on NVC, the IIF pattern was fine-speckled + nucleolar (Scl-70+) in 44%, centromeric in 33%, nucleolar in 11%, and homogeneous in 11% of patients with SSc. Antitopoisomerase I antibodies were significantly more frequent (57%) in patients with late pattern of microangiopathy on NVC. The median time of progression from early to active disease was significantly lower in patients with both fine-speckled + nucleolar and nucleolar ANA positivity. The severity of microangiopathy was higher in patients with the nucleolar pattern on IIF. Patients already showing a slight reduction of capillary number at baseline were likely to have either the nucleolar or the fine-speckled + nucleolar pattern on IIF. Of note, 37% of patients still showing the early microangiopathy pattern on NVC at the end of the followup were ANA-negative.Conclusion.ANA-negative patients with SSc display a slower progression of nailfold microangiopathy characterized by the early pattern on NVC. Progression to the late NVC pattern (more advanced stage of microvascular damage) seems to be associated with a different autoantibody pattern on IIF (fine-speckled + nucleolar pattern being the most prevalent).

scholarly journals Antinuclear antibody titer and antibodies to extractable nuclear antigens

2005 ◽  
Vol 53 (6) ◽  
pp. 987-988 ◽  
Author(s):  
Xavier Bossuyt ◽  
Ann Hendrickx ◽  
Johan Frans
Keyword(s):  
Antibody Titer ◽  
Antinuclear Antibody ◽  
Nuclear Antigens

scholarly journals Evaluation of Antibody Response in Sows after Vaccination with Senecavirus A Vaccine and the Effect of Maternal Antibody Transfer on Antibody Dynamics in Offspring

Vaccines ◽  
2021 ◽  
Vol 9 (10) ◽  
pp. 1066
Author(s):  
Fan Yang ◽  
Zixiang Zhu ◽  
Huanan Liu ◽  
Weijun Cao ◽  
Wei Zhang ◽  
...  
Keyword(s):  
Antibody Response ◽  
Neutralizing Antibodies ◽  
Low Dose ◽  
Active Immunization ◽  
High Dose ◽  
Booster Immunization ◽  
Substantial Problem ◽  
Antibody Levels ◽  
And Control ◽  
Antibody Dynamics

Senecavirus A (SVA) is a newly porcine virus that has been detected in many countries since its first detection in pigs in Canada in 2007, and it remains endemic in many countries in Asia and America, which has become a substantial problem for the pig industry. Vaccination is a potentially effective strategy for the prevention and control of SVA infection. Our lab has developed a SVA vaccine candidate previously. In this study, the antibody response to the prepared vaccine in sows and their offspring was evaluated. Vaccination of sows with inactivated SVA vaccines during pregnancy elicited SVA-specific virus-neutralizing antibodies. Vaccination with a high dose of SVA vaccine followed a booster immunization contributed to a long-term duration of the persistence of maternally derived neutralizing antibodies (MDAs) in the milk of the sows (>14 days). In contrast, vaccination with a single low dose of SVA vaccine resulted in a short-term persistence of MDAs in the milk (2–7 days). The MDAs could be efficiently transferred from the sows to their offspring through the colostrum/milk but not the umbilical cord blood. The antibody titers and the duration of the persistence of MDAs in the offspring are highly associated with the antibody levels in the milk from the sows. Vaccination of sows with a booster dose of SVA vaccine resulted in a longer-lasting MDAs in their offspring (persisted for at least 90 days). However, vaccination with the single low dose of vaccine only brought about 42 days of MDAs persistence in their offspring. The effect of MDAs on active immunization with SVA vaccine in offspring was further evaluated, which showed that vaccination of the SVA vaccine in the presence of MDAs at the titer of ≈1:64 or less could overcome the MDAs’ interference and give rise to effective antibody response. This will help for establishing the optimal times and schedules for SVA vaccination in pigs.

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