scholarly journals STUDIES ON THE LIFE HISTORY OF LYMPHOCYTES

1971 ◽  
Vol 133 (5) ◽  
pp. 1131-1148 ◽  
Author(s):  
Peter C. Nowell ◽  
Darcy B. Wilson
Keyword(s):  
Life History ◽  
Tritiated Thymidine ◽  
The Body ◽  
Lymphoid Tissues ◽  
Future Studies ◽  
In Vivo Labeling ◽  
History Of ◽  
X Irradiation ◽  
Mitotic Figures

The life history, within the rat, of lymphocytes responsive to histocompatibility isoantigens in the mixed lymphocyte interaction was examined by the use of in vivo labeling with tritiated thymidine and radioautography. Lymphocytes in the peripheral blood and H-ARC (mitotic figures in the MLI) were compared with respect to the frequency of labeled cells and the median grain count. The following conclusions were drawn from this study: (a) Although some can be considered long-lived, the majority of H-ARC are the products of recent divisions in the body. (b) Adult thymectomy does not eliminate the production of long-lived lymphocytes, some of which are H-ARC. Hence, in addition to direct origin in the thymus, H-ARC, as well as other lymphocytes of the long-lived lymphocyte population, may derive from already existing thymus-derived cells in the circulation and thymus-dependent areas of the secondary lymphoid tissues. (c) Sublethal X-irradiation (600 R) in combination with adult thymectomy does not eliminate the capacity to produce some long-lived lymphocytes, however, few if any are H-ARC. (d) H-ARC and other long-lived lymphocytes appear to go through a series of rapid multiple divisions before they enter the circulation. Thereafter, long-lived lymphocytes appear to undergo intermittent single divisions which decrease both the frequency and median grain count of labeled cells gradually with time. On the other hand, labeled H-ARC maintain a more stable grain count despite a rapid decrease in frequency with time. This is taken to indicate that H-ARC are less likely to undergo occasional single divisions during their life-span, but may undergo periodic rapid sequential divisions. A speculative model is developed from these data on the life history of H-ARC which may be of predictive value in future studies and which can be tested against known facts.

scholarly journals Proliferation in vivo of T lymphocytes reactive to histocompatibility alloantigens: a correction.

1975 ◽  
Vol 142 (1) ◽  
pp. 230-235 ◽  
Author(s):  
P C Nowell ◽  
J B Finan ◽  
D B Wilson
Keyword(s):  
Life History ◽  
Peripheral Blood ◽  
Tritiated Thymidine ◽  
Age Distribution ◽  
Blood Cultures ◽  
Lymphocyte Population ◽  
Rat Lymphocytes ◽  
History Of ◽  
Distribution In Blood

Rat lymphocytes in mixed cultures can reutilize tritiated thymidine from labeled granulocytes. Shortly after thymidine injections in vivo, major effects on the frequency of labeled lymphocyte mitoses in peripheral blood cultures are introduced by 10-20% polymorph contamination, even though transfer of label via supernates is not demonstrable. Cold thymidine in the cultures prevents reutilization, and has permitted reevaluation of several previous conclusions concerning the life history of lymphocytes reactive to major histocompatibility alloantigens (HARC). Rather than being predominantly recently divided cells, HARC do not appear to have an age distribution, in blood or lymph, significantly different from the general recirculating lymphocyte population. However, the ability of immunization across strong allogeneic differences to increase markedly the proportion of young HARC among the specifically responsive population has been confirmed.

scholarly journals Differences in cell cycle characteristics among patients with acute nonlymphocytic leukemia

Blood ◽  
1987 ◽  
Vol 69 (6) ◽  
pp. 1647-1653 ◽  
Author(s):  
A Raza ◽  
Y Maheshwari ◽  
HD Preisler
Keyword(s):  
Cell Cycle ◽  
Tritiated Thymidine ◽  
S Phase ◽  
Total Cell ◽  
Acute Nonlymphocytic Leukemia ◽  
Cell Cycle Time ◽  
Myeloid Leukemias ◽  
History Of

The proliferative characteristics of myeloid leukemias were defined in vivo after intravenous infusions of bromodeoxyuridine (BrdU) in 40 patients. The percentage of S-phase cells obtained from the biopsies (mean, 20%) were significantly higher (P = .00003) than those determined from the bone marrow (BM) aspirates (mean, 9%). The post- BrdU infusion BM aspirates from 40 patients were incubated with tritiated thymidine in vitro. These double-labeled slides were utilized to determine the duration of S-phase (Ts) in myeloblasts and their total cell cycle time (Tc). The Ts varied from four to 49 hours (mean, 19 hours; median, 17 hours). Similarly, there were wide variations in Tc of individual patients ranging from 16 to 292 hours (mean, 93 hours; median, 76 hours). There was no relationship between Tc and the percentage of S-phase cells, but there was a good correlation between Tc and Ts (r = .8). Patients with relapsed acute nonlymphocytic leukemia (ANLL) appeared to have a longer Ts and Tc than those studied at initial diagnosis. A subgroup of patients at either extreme of Tc were identified who demonstrated clinically documented resistance in response to multiple courses of chemotherapy. We conclude that Ts and Tc provide additional biologic information that may be valuable in understanding the variations observed in the natural history of ANLL.

On the life history of Ascaris devosi and its development in the white mouse and the domestic ferret

Parasitology ◽  
1953 ◽  
Vol 42 (3-4) ◽  
pp. 244-258 ◽  
Author(s):  
J. F. A. Sprent
Keyword(s):  
Life History ◽  
Intermediate Host ◽  
White Mouse ◽  
The Body ◽  
Stage Larva ◽  
Evolutionary Development ◽  
Food Material ◽  
Mature Adult ◽  
Mouse Tissues ◽  
History Of

The development of Ascaris devosi, a parasite of the fisher and marten, was followed from the egg to the adult stage using the white mouse and the ferret as the intermediate and final hosts respectively. The eggs contained motile 1st stage larvae 6 days after cleavage and were infective at 12 days, the 1st moult having already occurred. The eggs remained infective for at least 1 year. The 2nd stage larva after hatching from the egg in the intestine of the mouse passes through the intestinal wall to the liver and mesenteric tissues. At 3 days after infection they were recovered from the heart, lungs, brain, kidneys and from the carcass. The larvae grow and store food material during the 2nd stage and between 8 and 12 days after infection they undergo the 2nd moult. The mouse shows the most severe pulmonary symptoms on the 3rd and 4th days after infection, the lungs showing complete red hepatization at this time. The 3rd stage larva is relatively inactive and becomes encapsulated in various tissues, particularly in the muscular and subcutaneous tissues of the neck, shoulders and thorax. The chief developmental changes, apart from growth, which occur in the 2nd and 3rd stage larvae are: (i) the intestine develops from a single row of cells to a multi-cellular tube; (ii) the body cavity appears; (iii) the excretory lobes appear, the nucleus on the left side becoming prominent at the end of the 2nd stage; (iv) the cuticle shows transverse striations at the end of the 2nd stage; (v) the lateral lines become prominent.The encapsulated 3rd stage larvae remained alive for at least six months in the tissues of mice and at 25 days after infection of the mouse they were able to develop in the young ferret following killing and ingestion of the mouse. No infection of ferrets was obtained through oral administration of embryonated eggs or 3rd stage larvae digested from mouse tissues.The 3rd moult occurred in the intestine of the young ferret 3–4 days after infection; in adult ferrets the 3rd stage larvae were evidently unable to gain a hold and were passed out in the faeces. In the next 2–3 weeks the larva grew from about 2 to 16 mm. the 4th moult occurring between 2 and 3 weeks after infection. During the 4th stage the lips develop into the adult form and sexual differentiation occurs. In the female the genital rudiment moves forward and becomes differentiated into the vagina, uteri and ovaries. The vulva remains closed throughout the 4th stage.The adult parasites had developed to sexual maturity by 56 days after infection, but they continued to grow and were considerably longer at 6 months after infection. The position of the vulva relative to the body length was found to move from about midway along the body in the 4th stage larva to a position at the junction of the anterior and middle third of the body in the mature adult.The life history of this parasite is discussed in relation to that of A. lumbricoides and other species. It is considered that the life history of A. devosi, requiring as it does a true intermediate host for its completion, provides further information on the evolutionary development of the ascaris group. This work accordingly supports the hypothesis that the earliest members of this group utilized an intermediate host and does not support that which supposes that ascaris parasites are descended from skin-penetrating forms.During this investigation the writer has benefited considerably from correspondence with Dr J. D. Tiner, Department of Zoology, University of Illinois, Urbana, Illinois, U.S.A. His thanks are also due to Dr H. B. Speakman and Dr A. M. Fallis for their encouragement, guidance and help.This work was supported by the Province of Ontario on the recommendation of the Research Council of Ontario.Grateful acknowledgement is made to Mr Cliff Smith of the Connaught Medical Research Laboratories of the University of Toronto for photographic work.

On the Morphology and Life History of Fasciola nyanzae Leiper, 1910 from the Hippopotamus

1961 ◽  
Vol 35 (S1) ◽  
pp. 53-62 ◽  
Author(s):  
J. A. Dinnik ◽  
N. N. Dinnik
Keyword(s):  
Life History ◽  
New Species ◽  
Bile Ducts ◽  
The Body ◽  
Characteristic Shape ◽  
History Of ◽  
Liver Flukes ◽  
A New Species

A half a century ago, R. T. Leiper described a new species belonging to the genus Fasciola from specimens which he collected from hippopotami at the Murchison Falls, on the Victoria Nile in Uganda, in the summer of 1907. This species, Fasciola nyanzae, was found in the bile ducts of the liver, and in most instances the specimens were somewhat macerated owing to the length of time that had elapsed before the animal could be recovered from the water. Nevertheless, the characteristic shape of these liver flukes and the restriction of the branched testes to the anterior third of the body made it possible to recognise them as being different from all other species of the genus known at that time.

scholarly journals THE LIFE-HISTORY OF THE SPINED RUSTIC, BARATHRA CURIALIS, SMITH

1906 ◽  
Vol 38 (12) ◽  
pp. 381-384
Author(s):  
James Fletcher ◽  
Arthur Gibson
Keyword(s):  
Life History ◽  
Nova Scotia ◽  
Lake Superior ◽  
The Body ◽  
Wide Area ◽  
History Of

In the Report of the Entomologist and Botanist to the Dominion Experimental Farms for 1905, at pages 179 and 180, considerable space is given to a discussion of an outbreak of a large noctuid caterpillar, which appeared in considerable numbers in Canada during 1905. Complaints of injury by this insect were received from a wide area, extending from Nova Scotia as far west as Lake Superior. During July many kinds of plants in gardens were attacked by smooth cutworm-like caterpillas, which when small were greenish in colour, having the body divided into two equal areas above and below the spiracles by a wide black stigmatal band.

Fasting and Feasting: Paradoxes of the Sport Ethic

1998 ◽  
Vol 15 (1) ◽  
pp. 41-63 ◽  
Author(s):  
David Johns
Keyword(s):  
Life History ◽  
The Body ◽  
International Competition ◽  
Philosophical Concept ◽  
History Of ◽  
Rhythmic Gymnast

In this study the sociological and philosophical concept of the sport ethic has been utilized to explain the meaning of extreme and overconforming athlete behaviors which manifest themselves as athletic preparation. The study discloses, through the life history of a rhythmic gymnast, how the meanings and values of what it means to be an athlete were transmitted through the day-to-day discourse of athlete practice. By focusing on the dietary preoccupations of gymnasts involved in international competition, it was possible to demonstrate how modern sport preparation is not only distorted but also paradoxical, serving to push the body beyond its limits while insisting on its preservation.

scholarly journals A SINGULAR CASE

1870 ◽  
Vol 2 (4) ◽  
pp. 43-44
Author(s):  
V. T. Chambers
Keyword(s):  
United States ◽  
Life History ◽  
Black Spot ◽  
The United States ◽  
The Body ◽  
Singular Case ◽  
Mature Larva ◽  
History Of ◽  
Walnut Leaves

Seeing in the last number of the Canadian Entomologist, a description of the egss of A. Luna, reminds me to ask of you the explanation of a curious circumstance in the life-history of one bred by me from the larva last year. I will premise that I am writing without my notes, and therefore cannot give figures accurately, but can give the facts. There may be nothing very strange about it, but two of the best entomologists in the United States inform me that it is entirely new to them. It is this:–Some time in the latter part of the summer of 1868 I took, feeding on walnut leaves, a mature larva of A. Luna; from which I did not houi to rear the mature insect, because I counted on the larva over twenty eggs like those of a Tachina, Underneath some of the eggs I could discern with a lens a minute opening through which the fly-larva had entered the body of the Luna larva. The skin of the latter was more or less discoloured under each egg, but under some-under many in fact there was a dense black spot, sometimes two lines in diameter.

scholarly journals On a convenient terminology for the various stages of the malaria parasite

1902 ◽  
Vol 70 (459-466) ◽  
pp. 74-79
Keyword(s):  
Life History ◽  
Natural History ◽  
Human Blood ◽  
Malaria Parasite ◽  
Point Of View ◽  
The Body ◽  
History Museum ◽  
General Morphology ◽  
History Of ◽  
Reproductive Phases

I have found it necessary in labelling a series of models of the malaria parasite in the Central Hall of the Natural History Museum to use as simple and clear a terminology as possible. I think that this terminology will be found useful by others who are perplexed by such terms as “sporozoites,” “blasts,” “ookinetes,” “schizonts,” “amphionts,” and “sporonts”—terms which have their place in schemes dealing with the general morphology and life-history of the group Sporozoa, but are not, as experience shows, well suited for immediate use in describing and referring to the stages of the malaria parasite. It is necessary to treat the malaria parasite from the point of view of malaria; that is to say, to consider its significant phases to be those which it passes in the human blood. In reality its mature condition and most important motile, as well as its most prolific reproductive, phases are passed in the body of the mosquito.

scholarly journals Mosquito Trypanosomes

1906 ◽  
Vol 6 (2) ◽  
pp. 110-111 ◽  
Author(s):  
F. G. Novy ◽  
W. J. Macneal ◽  
H. N. Torrey
Keyword(s):  
Life History ◽  
Test Tube ◽  
Blood Agar ◽  
Intracellular Parasites ◽  
History Of

In a previous paper on Bird Trypanosomes it was pointed out that these organisms grew readily in the test-tube on blood agar and that the resulting forms resembled the flagellates which Schaudinn found in the gut of mosquitoes which had fed on owls infected with Halteridium and with H. Ziemanni. In other words, the position taken was that the flagellates observed in the mosquitoes did not represent stages in the life-history of intracellular parasites but were actually cultures in vivo of trypanosomes present in the blood of the birds used. In confirmation of this position it was desirable to show that trypanosomes could actually grow and multiply in the gut of mosquitoes and that such forms actually did correspond to those which would be obtained in vitro.

scholarly journals O corpóreo e a erupção da bestialidade em Carne e Sozinho Contra Todos, de Gaspar Noé // The bodily and the eruption of bestiality in Meat and I Stand Alone, by Gaspar Noé

2019 ◽  
Vol 16 (3) ◽  
pp. 786
Author(s):  
Josette Alves de Souza Monzani ◽  
Mario Sergio Righetti
Keyword(s):  
Life History ◽  
The Body ◽  
The Social ◽  
History Of ◽  
Historia De Vida ◽  
The Spectator ◽  
The Aesthetic ◽  
Aesthetic Procedures

Resumo: Procura-se apontar de que modo os procedimentos estéticos do diretor franco-argentino Gaspar Noé acabam por realizar uma proposta de cinematografia radical que estabelece uma tensão no compartilhamento sensorial entre o corpo da tela, o corpo da câmera e o corpo do espectador. Carne (1991) e Sozinho Contra Todos (1998) narram a história de vida do Açougueiro, um cidadão à margem da sociedade metropolitana francesa, marcada por represamentos afetivos que o comprimem e levam a cometer ações bestiais marcadas por ‘enganos’, equívocos que o prejudicarão para sempre.O corpo cinematográfico de Noé faz uso da imagem e do som hápticos, trilhando um caminho batailleano - através da transgressão, da sensorialidade e da experimentação do abjeto -, e através deles busca induzir no espectador o que denominamos afeto carnal e corporeidade imanente como forma de pontuar as questões sociais e éticas propostas pelo diretor. Abstract: It is tried to point out how the aesthetic procedures of the French-Argentine director Gaspar Noé end up making a proposal of radical cinematography that establishes a tension in the sensorial sharing among the body of the screen, the body of the camera and the body of the spectator. Meat (1991) and I Stand Alone (1998) chronicle the life history of the Butcher, a citizen on the fringes of French metropolitan society, marked by affective imprisonments that compel him to commit bestial actions marked by ‘mistakes’, mistakes that will do him harm forever. The cinematographic body of Noé makes use of haptic image and sound, traversing a Bataillean path - through transgression, sensoriality and experimentation of the abject - and by them seeks to induce in the viewer what we call carnal affection and immanent corporeity as a way of punctuating the social and ethical questions proposed by the director.

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