scholarly journals Direct demonstration of an HLA-DR allotypic determinant on the low molecular weight (beta) subunit using a mouse monoclonal antibody specific for DR3

1982 ◽  
Vol 156 (1) ◽  
pp. 104-111 ◽  
Author(s):  
JP Johnson ◽  
T Meo ◽  
G Reithmuller ◽  
DJ Schendel ◽  
R Wank
Keyword(s):  
Molecular Weight ◽  
Monoclonal Antibody ◽  
Cell Surface ◽  
B Cell ◽  
Beta Subunit ◽  
Low Molecular Weight ◽  
Β Subunit ◽  
Direct Demonstration ◽  
Hla Dr ◽  
Human B Cell

A murine monoclonal antibody directed against a human B cell surface antigen with the characteristics of HLA-DR is described. The antigen detected is tightly linked to HLA and is correlated with the alloantigen HLA-Dw/DR3. Reactivity with a fraction of Dw/DRw6 cells is also observed. The determinant recognized by this antibody has been shown to be present on the smaller molecular weight β subunit of the HLA-DR antigen.

scholarly journals B8.7 antigen is present on B-cell precursor acute lymphocytic leukemia. Correlation with the low molecular weight B-cell growth factor responsiveness of these cells

Blood ◽  
1990 ◽  
Vol 75 (4) ◽  
pp. 963-971 ◽  
Author(s):  
C Leprince ◽  
N Blumenfeld ◽  
G Flandrin ◽  
P Galanaud ◽  
F Sigaux ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Growth Factor ◽  
Cell Growth ◽  
B Cell ◽  
Lymphocytic Leukemia ◽  
Low Molecular Weight ◽  
Dependent Manner ◽  
Cell Precursor ◽  
B Cell Growth Factor ◽  
Human B Cell

Abstract The purpose of this study was to analyze the expression of B8.7 antigen and its implication in the low molecular weight B-Cell growth factor (LMW BCGF) proliferative pathway at the early stages of the human B- cell differentiation. After an overnight incubation in culture medium of B-cell precursor acute lymphoblastic leukemias (ALL), we demonstrated the presence of B8.7 antigen in 18 of 25 cases (72%). Such an incubation also induced a significant increase in the LMW BCGF responsiveness of ALL cells (P less than 0.03). In addition, we showed a significant correlation between B8.7 expression and the ability of pre-B ALL cells to respond to LMW BCGF. As previously described for normal B cells, the anti-B8.7 monoclonal antibody inhibited the LMW BCGF-dependent proliferation of pre-B ALL cells in a dose-dependent manner. These data indicate that B8.7 antigen is expressed and may be functionally related to the LMW BCGF pathway at the pre-B cell stages of differentiation. These results also suggest that human B-cell precursor ALL are not only phenotypically similar to their normal B lymphocyte counterparts, but are also sensitive to the same immunoregulatory cytokines that control normal cell growth.

scholarly journals Human B cell variants immunoselected against a single Ia antigen subset have lost expression of several Ia antigen subsets.

1983 ◽  
Vol 157 (3) ◽  
pp. 1053-1058 ◽  
Author(s):  
R S Accolla
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
B Cell ◽  
Cell Line ◽  
Lymphoblastoid Cell Line ◽  
Antigenic Determinants ◽  
Ia Antigen ◽  
Hla Dr ◽  
Surface Immunoglobulins ◽  
Human B Cell

Two monoclonal antibodies, D1-12 and BT 2.2, recognizing two distinct subsets of human Ia molecules, NG1 and NG2, respectively, present in all individuals irrespective of their HLA-DR phenotype, have been used to immunoselect cell variants from the lymphoblastoid cell line Raji. Results showed that, irrespective of the monoclonal antibody used for immunoselection, the cell variants analyzed in this study had lost the expression of both D1-12-and BT 2.2-specific antigenic determinants. Moreover, the expression of antigenic determinants specific for a third family of Ia molecules, the DC-1 subset, were also lost in the cell variants. In contrast, expression of HLA A, B, and C common structures, as recognized by the W6.32 monoclonal antibody, as well as expression of surface immunoglobulins, were not affected. Possible mechanisms inducing such a coordinate loss of expression of several families of human Ia molecules are discussed.

scholarly journals B8.7 antigen is present on B-cell precursor acute lymphocytic leukemia. Correlation with the low molecular weight B-cell growth factor responsiveness of these cells

Blood ◽  
1990 ◽  
Vol 75 (4) ◽  
pp. 963-971
Author(s):  
C Leprince ◽  
N Blumenfeld ◽  
G Flandrin ◽  
P Galanaud ◽  
F Sigaux ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Growth Factor ◽  
Cell Growth ◽  
B Cell ◽  
Lymphocytic Leukemia ◽  
Low Molecular Weight ◽  
Dependent Manner ◽  
Cell Precursor ◽  
B Cell Growth Factor ◽  
Human B Cell

The purpose of this study was to analyze the expression of B8.7 antigen and its implication in the low molecular weight B-Cell growth factor (LMW BCGF) proliferative pathway at the early stages of the human B- cell differentiation. After an overnight incubation in culture medium of B-cell precursor acute lymphoblastic leukemias (ALL), we demonstrated the presence of B8.7 antigen in 18 of 25 cases (72%). Such an incubation also induced a significant increase in the LMW BCGF responsiveness of ALL cells (P less than 0.03). In addition, we showed a significant correlation between B8.7 expression and the ability of pre-B ALL cells to respond to LMW BCGF. As previously described for normal B cells, the anti-B8.7 monoclonal antibody inhibited the LMW BCGF-dependent proliferation of pre-B ALL cells in a dose-dependent manner. These data indicate that B8.7 antigen is expressed and may be functionally related to the LMW BCGF pathway at the pre-B cell stages of differentiation. These results also suggest that human B-cell precursor ALL are not only phenotypically similar to their normal B lymphocyte counterparts, but are also sensitive to the same immunoregulatory cytokines that control normal cell growth.

Cell surface phenotype, cytokines, and antibody gene expression in immortalized human B cell lines

1990 ◽  
Vol 1 (3) ◽  
pp. 145-153 ◽  
Author(s):  
K. James ◽  
J. Gardner ◽  
G. Skibinski ◽  
M. McCann ◽  
R. Thorpe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Surface ◽  
B Cell ◽  
Cell Lines ◽  
Surface Phenotype ◽  
Human B Cell ◽  
Antibody Gene

Initial experience with treatment of human B cell lymphoma with anti-CD19 monoclonal antibody

1991 ◽  
Vol 32 (6) ◽  
pp. 364-372 ◽  
Author(s):  
A. Hekman ◽  
A. Honselaar ◽  
W. M. J. Vuist ◽  
J. J. Sein ◽  
S. Rodenhuis ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
B Cell ◽  
Cell Lymphoma ◽  
B Cell Lymphoma ◽  
Initial Experience ◽  
Human B Cell

scholarly journals Induction of human IgE synthesis by CD4+ T cell clones. Requirement for interleukin 4 and low molecular weight B cell growth factor.

1989 ◽  
Vol 170 (5) ◽  
pp. 1477-1493 ◽  
Author(s):  
R H DeKruyff ◽  
T Turner ◽  
J S Abrams ◽  
M A Palladino ◽  
D T Umetsu
Keyword(s):  
Molecular Weight ◽  
T Cells ◽  
T Cell ◽  
B Cells ◽  
B Cell ◽  
Cd4 T Cell ◽  
Low Molecular Weight ◽  
Cell Clones ◽  
B Cell Growth Factor ◽  
Ige Synthesis

We have analyzed in detail the precise requirements for the induction of human IgE synthesis using several experimental approaches with purified B cells and well-characterized alloantigen-specific CD4+ T cell clones expressing different profiles of lymphokine secretion. Using these clones under cognate conditions in which the B cells expressed alloantigens recognized by the cloned T cells, we have confirmed that IL-4 is required for the induction of IgE synthesis, but we have clearly demonstrated that IL-4 by itself is not sufficient. With several cloned CD4+ T cell lines, including an IL-4-producing clone that could not induce IgE synthesis, and cloned T cells pretreated with cyclosporin A to inhibit lymphokine synthesis, we showed that Th cell-B cell interactions are necessary for IgE synthesis, and that low molecular weight B cell growth factor (LMW-BCGF) and IL-4, in combination, are lymphokines of major importance in the induction of IgE synthesis. Together our results indicate that optimal induction of an IgE-specific response requires the exposure of B cells to a particular complex of signals that include (a) a signal(s) involving Th-B cell interaction that primes B cells to receive additional signals from soluble lymphokines, (b) a specific B cell proliferative signal provided by LMW-BCGF, and (c) a specific B cell differentiation signal provided by IL-4.

Different human B cell subsets respond to interleukin 2 and to a high molecular weight B cell growth factor (BCGF)

1986 ◽  
Vol 16 (12) ◽  
pp. 1503-1507 ◽  
Author(s):  
Aime Vazquez ◽  
Jean-Philipe Gerard ◽  
Daniel Olive ◽  
Marie-Thérèse Auffredou ◽  
Bernard Dugas ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Growth Factor ◽  
Cell Growth ◽  
B Cell ◽  
High Molecular Weight ◽  
Interleukin 2 ◽  
B Cell Growth Factor ◽  
B Cell Subsets ◽  
Human B Cell
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