scholarly journals Transition from interleukin 1 beta (IL-1 beta) to IL-1 alpha production during maturation of inflammatory macrophages in vivo.

1992 ◽  
Vol 175 (6) ◽  
pp. 1793-1797 ◽  
Author(s):  
H U Beuscher ◽  
U P Rausch ◽  
I G Otterness ◽  
M Röllinghoff
Keyword(s):  
Bacterial Infection ◽  
Yersinia Enterocolitica ◽  
Interleukin 1 ◽  
Peyer's Patches ◽  
Interleukin 1 Beta ◽  
Alpha Production ◽  
Inflammatory Macrophages ◽  
In Situ Production

In situ production of interleukin 1 alpha (IL-1 alpha) and IL-1 beta was investigated in Peyer's patches (PP) of mice undergoing an acute bacterial infection with Yersinia enterocolitica O8. Synthesis of IL-1 beta, as determined by immunohistochemistry, was found primarily in monocytes migrating into the inflamed PP. In comparison, synthesis of IL-1 alpha was temporarily delayed by at least 24 h and was only found in mature macrophages, which did not produce detectable levels of IL-1 beta. This indicates a transition from IL-1 beta to IL-1 alpha production during maturation of monocytes into inflammatory macrophages, and further emphasizes a dichotomy between IL-1 alpha and IL-1 beta.

Is the Success of Cefazolin plus Ertapenem in Methicillin-Susceptible Staphylococcus aureus Bacteremia Based on Release of Interleukin 1-beta?

2022 ◽  
Author(s):  
Dan Smelter ◽  
Mary Hayney ◽  
George Sakoulas ◽  
Warren Rose
Keyword(s):  
Staphylococcus Aureus ◽  
Peripheral Blood ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Blood Monocytes ◽  
Salvage Regimen ◽  
Peripheral Blood Monocytes ◽  
Staphylococcus Aureus Bacteremia

Cefazolin and ertapenem has been shown to be an effective salvage regimen for refractory methicillin-susceptible Staphylococcus aureus bacteremia. Our findings suggest cefazolin plus ertapenem in vitro stimulates interleukin-1β release from peripheral blood monocytes both with and without S. aureus presence. This IL-1β augmentation was primarily driven by ertapenem. These findings support further exploration of cefazolin plus ertapenem in MSSA bacteremia and may partially explain its marked potency in vivo despite modest synergy in vitro .

scholarly journals The human interleukin-1 alpha gene is located on the long arm of chromosome 2 at band q13

Blood ◽  
1989 ◽  
Vol 73 (1) ◽  
pp. 104-107 ◽  
Author(s):  
M Lafage ◽  
N Maroc ◽  
P Dubreuil ◽  
R de Waal Malefijt ◽  
MJ Pebusque ◽  
...  
Keyword(s):  
Interleukin 1 ◽  
Cdna Probe ◽  
Proximal Part ◽  
Chromosome 2 ◽  
Interleukin 1 Beta ◽  
Hematopoietic Growth Factors ◽  
Alpha Gene ◽  
Immunologic Reactions ◽  
Beta Gene

Abstract Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which was described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors. In this report we discuss our use of in situ hybridization on human prometaphase cells with a human IL-1 alpha cDNA probe to localize the human IL-1 alpha gene on the proximal part of the long arm of chromosome 2 at band q13, in the same chromosomal region as the IL-1 beta gene.

A H2O2-free depot for treating bacterial infection: localized cascade reactions to eradicate biofilms in vivo

Nanoscale ◽  
2018 ◽  
Vol 10 (37) ◽  
pp. 17656-17662 ◽  
Author(s):  
Zhengqing Yan ◽  
Wei Bing ◽  
Chao Ding ◽  
Kai Dong ◽  
Jinsong Ren ◽  
...  
Keyword(s):  
Bacterial Infection ◽  
Cascade Reactions ◽  
Main Components

H2O2-free depots (CaO2/H-G@alginate) can produce hROS in situ through localized cascade reactions, damaging the main components of biofilms.

Macrophage Cell Activation with Acute Apical Abscess Contents Determined by Interleukin-1 Beta and Tumor Necrosis Factor Alpha Production

2014 ◽  
Vol 40 (11) ◽  
pp. 1752-1757 ◽  
Author(s):  
Ezilmara L.R. Sousa ◽  
Frederico C. Martinho ◽  
Fabio R.M. Leite ◽  
Gustavo G. Nascimento ◽  
Brenda P.F.A. Gomes
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Cell Activation ◽  
Interleukin 1 ◽  
Interleukin 1 Beta ◽  
Macrophage Cell ◽  
Necrosis Factor Alpha ◽  
Alpha Production ◽  
Factor Alpha ◽  
Necrosis Factor

The Histone Deacetylase-8 (HDAC8) Selective Inhibitor PCI-34051 Decreases Interleukin-1 Beta Secretion in Vitro and Reduces Inflammation in Vivo

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 2581-2581
Author(s):  
Sriram Balasubramanian ◽  
Susanne Steggerda ◽  
Mint Sirisawad ◽  
Marshall Schreeder ◽  
Luke Doiron ◽  
...  
Keyword(s):  
T Cell ◽  
Secretory Pathway ◽  
Interleukin 1 ◽  
Contact Hypersensitivity ◽  
Secretory Process ◽  
Mrna Levels ◽  
Interleukin 1 Beta ◽  
Caspase 1 ◽  
T Cell Lymphomas

Abstract Inhibitors of histone deacetylases (HDACs) which are currently in clinical testing for treating various cancers typically inhibit multiple isoforms of the 11-member HDAC family. We have developed an isoform-selective HDAC inhibitor, PCI-34051, that inhibits HDAC8 with a Ki of 10 nM and greater than 200-fold selectivity over other HDAC isoforms (Balasubramanian et al. (2008) Leukemia,22:1026–34). We have shown that PCI-34051 selectively induced apoptosis in cell lines derived from T-cell lymphomas and leukemias, but not in other tumor or normal cell types. Here we show that it potently inhibits the secretion of the pro-inflammatory cytokine interleukin-1 beta (IL-1b) in lipopolysaccharide (LPS)-stimulated peripheral mononuclear blood cells (PBMC) and isolated monocytes. PCI-34051 inhibited IL-1b secretion (by 80% compared to control) from LPS-stimulated human PBMC with an IC50 of 0.6 uM, which is much lower than the growth inhibitory concentrations of 2.4–4 uM required in T-cell lymphomas. We found that PCI-34051 also inhibited the secretion of interleukin-18 (IL-18) to a similar extent as IL-1b, but secretion of other pro-inflammatory cytokines, including MIP-1b, MCP-1, TNFa, and IL-6, was inhibited to a smaller extent. Interestingly, IL-18, like IL-1b, is synthesized without a signal peptide, and also utilizes the same non-classical endosomal secretory pathway as IL-1b including cleavage of the pro-form by caspase-1. Thus, we theorized that the modulatory effect of PCI-34051 is likely to involve modulation of the post-translational secretory process. In accordance, we found that the IL-1b mRNA levels were reduced by only 20% compared to control, but the intracellular protein levels of the pro-form was increased by >50% in primary monocytes after treatment with PCI-34051, indicating that the mechanism was due to inhibition of the processing from the pro- to the mature form of the cytokine. We showed that this was not due to a direct inhibition of caspase-1 or TACE (TNF-alpha converting enzyme), but is likely due to an as-yet unidentified substrate of HDAC8. In vivo, PCI-34051 inhibited ear swelling induced by oxazolone in a model of contact hypersensitivity in mice, and we showed that this was accompanied by a reduction in IL-1b at both the protein and mRNA levels. Based on this result, we examined the effect of PCI-34051 on IL-1b secretion in human keratinocytes, as well as in PBMC from psoriasis patients, and found that it could reduce IL-1b secretion in both. We found that PCI-34051 decreased IL-1b by 60% in LPS-stimulated PBMC from rheumatoid arthritis (RA) patients, but the pan-HDAC inhibitors which were only weakly inhibitory to HDAC8 did not have this effect, indicating a specific role for HDAC8 in the secretory process. Finally, we found that in unstimulated PBMC from RA patients that had basal production of IL-1b that this could be decreased by 90% by treatment with PCI-34051. Taken together, these findings indicate that PCI-34051 is an active drug that could be useful for the treatment of T-cell lymphoma as well as for autoinflammatory diseases such as RA and psoriasis.

Aging and dietary modulation of elastase and interleukin-1 beta secretion

1995 ◽  
Vol 268 (1) ◽  
pp. R208-R213 ◽  
Author(s):  
J. G. Cannon ◽  
M. A. Fiatarone ◽  
M. Meydani ◽  
J. Gong ◽  
L. Scott ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Cell Function ◽  
Interleukin 1 ◽  
Lipid Peroxide ◽  
Arachidonic Acid Metabolism ◽  
Interleukin 1 Beta ◽  
Tension Development ◽  
Age Related

Aging is associated with diminished immune function that may stem from alterations in arachidonic acid metabolism and lipid peroxidation. This study sought to determine if dietary modification of fatty acids influenced neutrophil and monocyte secretion after an in vivo inflammatory stress in older human subjects. Volunteers participated in protocols that forced their quadriceps muscles to lengthen during tension development (eccentric stress). These protocols can cause inflammatory foci in the muscle as well as alterations in circulating leukocyte function. In this study, in vivo neutrophil degranulation was assessed by plasma elastase concentrations, and mononuclear cell function was assessed by interleukin-1 beta (IL-1 beta) secretion in vitro. In response to eccentric stress, older subjects (> 60 yr old) taking a placebo had no apparent elastase response, whereas those taking fish oil supplements responded with a 142% increase in plasma elastase (P = 0.011), similar to responses of younger reference subjects (< 33 yr old) taking no supplement. Overall, elastase responses correlated with individual plasma arachidonic acid-to-eicosapentaenoic acid ratios (r = -0.881, P = 0.004). Thus apparent age-related differences in elastase release were reconciled by individual differences in fatty acid nutriture. No significant temporal changes in urinary lipid peroxide excretion or IL-1 beta secretion were observed; however, age-associated differences were found.

Effect of fluoxetine on interleukin-1 beta in rat brain – in vivo and in vitro studies

2010 ◽  
Vol 62 ◽  
pp. 57
Author(s):  
Ewa Obuchowicz ◽  
Anna Bielecka ◽  
Agnieszka Prymus ◽  
Łukasz Drzyzga ◽  
Monika Paul-Samojedny ◽  
...  
Keyword(s):  
Rat Brain ◽  
Interleukin 1 ◽  
In Vitro Studies ◽  
Interleukin 1 Beta

Interleukin-1 potentiates glucose stimulated insulin release in the isolated perfused pancreas

1988 ◽  
Vol 117 (3) ◽  
pp. 302-306 ◽  
Author(s):  
Lise D. Wogensen ◽  
Thomas Mandrup-Poulsen ◽  
Helle Markholst ◽  
Jens Mølvig ◽  
Åke Lernmark ◽  
...  
Keyword(s):  
Insulin Release ◽  
Cell Function ◽  
Interleukin 1 ◽  
Systemic Circulation ◽  
Second Phase ◽  
Interleukin 1 Beta ◽  
Perfused Pancreas ◽  
Porcine Pancreas ◽  
Isolated Perfused Pancreas

Abstract. The acute effects of recombinant human interleukin-1 beta (rIL-1) on basal and glucose-stimulated insulin release were investigated in the isolated perfused pancreas. At a concentration of 20 μg/l rIL-1 had no effect on basal insulin release, but increased the total amount of insulin released during first and second phase insulin release in response to 20 mmol/l D-glucose in the rat pancreas (P < 0.05). In addition, 26 μg/l of rIL-1 potentiated insulin release in response to square wave infusions of stimulatory concentrations of glucose (11 mmol/l) in the porcine pancreas. We hypothesize that IL-1 in the systemic circulation may affect B cell function in vivo.

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