STRUCTURE, PARAMETERS AND FUNCTIONS OF SELF-RELATION

Self-attitude is a complex psychological phenomenon that characterizes the position of a person in relation to himself. The characteristics of self-attitude determine how adequately a person will be able to adapt in the sphere of his professional activity and affect his socially significant activity. Self-attitude is a kind of self-regulating mechanism that is present at all stages of behavioral activity, starting from the stage of motive formation and ending with the analysis of the result of activity.

Genetic Determinacy of the Behavioral Activity of the Silkworm (Bomby xmori L)

Тhe behavioral activity of such an extremely important agricultural object in scientific and practical terms as the silkworm still remains poorly studied. Aims: Сreation of silkworm lines with alternative behaviors to prove the genetic determinacy of the behavioral activity of the silkworm Bombyx mori L. Place and Duration of Study: The work was carried out at the Research Institute of Sericulture in the Laboratory of Genetics and Breeding of the silkworm in 2015-2020. The Ipakchi 1 and Ipakchi 2 breeds widely zoned in Uzbekistan were used as research objects. Methodology and Results: The Ipakchi 1 and Ipakchi 2 breeds widely zoned in Uzbekistan were used as research objects. The breeding of alternative silkworm lines in terms of motor activity was carried out by selecting the most active caterpillars and male butterflies in the "A" -lines (active), and the least active individuals in the "P"-lines (passive). The separation of individuals by motor activity led to their separation by reproductive and biological indicators. The difference between the lines by the average number of eggs in the clutch of the Ipakchi 1 breed in 2018 was 32 eggs, in 2020 already 133 eggs; by the mass of the clutch in 2018 – 25mg, in 2020 – 78mg. The same patterns were observed in the Ipakchi 2 breed. The greatest difference between the "A" and "P" lines was observed in the viability of caterpillars: in 2018 it was 3.2%, in 2020 already 6.9%. Conclusions: Selection of hatched larvae (caterpillars) and male butterflies by the speed of movement to the feed (the larvae) and to the sexual partner (butterflies) leads to the appearance of silkworm lines with alternative forms of motor activity.

Brain Perivascular Macrophages Do Not Mediate Interleukin-1-Induced Sickness Behavior in Rats

Sickness behavior, characterized by on overall reduction in behavioral activity, is commonly observed after bacterial infection. Sickness behavior can also be induced by the peripheral administration of Gram-negative bacterial lipopolysaccharide (LPS) or interleukin-1beta (IL-1β), a pro-inflammatory cytokine released by LPS-activated macrophages. In addition to the microglia, the brain contains perivascular macrophages, which express the IL-1 type 1 receptor (IL-1R1). In the present study, we assessed the role of brain perivascular macrophages in mediating IL-1β-induced sickness behavior in rats. To do so, we used intracerebroventricular (icv) administration of an IL-1β-saporin conjugate, known to eliminate IL-R1-expressing brain cells, prior to systemic or central IL-1β injection. Icv IL-1β-saporin administration resulted in a reduction in brain perivascular macrophages, without altering subsequent icv or ip IL-1β-induced reductions in food intake, locomotor activity, and social interactions. In conclusion, the present work shows that icv IL-1β-saporin administration is an efficient way to target brain perivascular macrophages, and to determine whether these cells are involved in IL-1β-induced sickness behavior.

Sex Matters: Effects of Sex and Mating in the Presence and Absence of a Protective Microbe

In most animals, female investment in offspring production is greater than for males. Lifetime reproductive success (LRS) is predicted to be optimized in females through extended lifespans to maximize reproductive events by increased investment in immunity. Males, however, maximize lifetime reproductive success by obtaining as many matings as possible. In populations consisting of mainly hermaphrodites, optimization of reproductive success may be primarily influenced by gamete and resource availability. Microbe-mediated protection (MMP) is known to affect both immunity and reproduction, but whether sex influences the response to MMP remains to be explored. Here, we investigated the sex-specific differences in survival, behavior, and timing of offspring production between feminized hermaphrodite (female) and male Caenorhabditis elegans following pathogenic infection with Staphylococcus aureus with or without MMP by Enterococcus faecalis. Overall, female survival decreased with increased mating. With MMP, females increased investment into offspring production, while males displayed higher behavioral activity. MMP was furthermore able to dampen costs that females experience due to mating with males. These results demonstrate that strategies employed under pathogen infection with and without MMP are sex dependent.

CURRENT ASPECTS OF FORMATION OF VALUE ATTITUDE TO OWN HEALTH IN MEDICAL STUDENTS

The issue of health-care personnel’s preservation of health is extremely urgent today. Especially now, during the coronavirus pandemic, the modern health care sector requires significant expenditure of psychophysical resources of medical professionals, given the need to perform their professional duties at different times of the day and with different degrees of mental and physical exertion. Therefore, starting from the student years, it is important to organize the educational process with special attention to mental, physical and social well-being of each student in particular. During university years, students begin to realize the importance of their own health. Even if students care about their health, they pay attention to its physical component, but psychological and social health is not given a proper place in the system of their values. With age, a personal concern for own health increases significantly. At the same time some contradictions arise, as with growing up the behavioral activity, which provides its support, and strengthening, decreases. In addition, the period of reduced activity is the result of psychological mechanisms of self-defense, in particular, to mask personal experiences related to health. The content of educational and professional programs of medical specialties purposefully and indirectly influences the formation of individual and professional values, the leading place among which is rightly occupied by health concerns. The level of education of medical students, which is obtained by mastering integrated, general and professional competencies, significantly depends on their personal lifestyle choices and habits that affect the preservation of their own mental and physical health. The level of working capacity of students depends on a set of different factors: daily routine, nutrition, rest, sleep, the presence of unfavorable conditions for mental work, the level of compliance with the rules of culture of mental behavior, according to age. Emphasizes the health benefits of outdoor activities. The use of information technology in education, of course, significantly increases the efficiency of the educational process, optimizing the assimilation of educational material. However, with each passing day, there is a growing need to create a specific security system in education to predict and monitor the impact of information technology on the health of students as there is a probable threat to health due to excessive use of computers and other household appliances with different types of electromagnetic radiation. The formation of a value attitude to own health is an important component of the educational process in higher medical educational institutions, which was emphasized in this article. The problem of value attitude to own health is of great importance in modern research and practice of organizing the educational activities of student youth. A young person’s attitude to own health depends on a number of factors, including age, social status and level of education. It is the medical students’ deep awareness of the specifics of mental and physical health, factors of pathogenic influence on their condition is an effective feature in formation of the value attitude to their own health and has a significant impact on their daily behavior, building a trajectory of maintaining their own health in future professional activities.

Relationship Between Subjective Ratings of Answers and Behavioral and Autonomic Nervous Activities During Creative Problem-Solving via Online Conversation

Creative problem solving has been important for the advent of new technologies. In this study, we hypothesized that subjective ratings of answers should be useful for evaluating the answer quality in creative problem solving. To test this hypothesis and extract objective indicators of the subjective ratings of answers, we evaluated the relationship between subjective ratings of task performance and behavioral and autonomic nervous activities during a creative problem-solving task performed via online conversation. The task involved an answerer and a supporter, and in the experiment, each pair performed 10 trials. The trials were categorized as highly or lowly rated according to the answerer’s confidence in the answer. The task performance and behavioral and autonomic nervous activities were then compared between these categories of trials. Behavioral activity was evaluated via movements and speech activities, while for autonomic nervous activity, sympathetic nervous activity (SNA) was evaluated via skin conductance. The task performance was significantly better in the highly rated trials, whereas there were no significant differences in the behavioral activities between the highly and lowly rated trials. Moreover, in the highly rated trials, the skin conductance of the answerer was significantly high, whereas that of the supporter was significantly low. The results support the hypothesis and suggest that contrasting differences in SNA between an answerer and a supporter are indicators of the subjective ratings of answers in creative problem solving.

Psilocin, LSD, mescaline, and DOB all induce broadband desynchronization of EEG and disconnection in rats with robust translational validity

Serotonergic psychedelics are recently gaining a lot of attention as a potential treatment of several neuropsychiatric disorders. Broadband desynchronization of EEG activity and disconnection in humans have been repeatedly shown; however, translational data from animals are completely lacking. Therefore, the main aim of our study was to assess the effects of tryptamine and phenethylamine psychedelics (psilocin 4 mg/kg, LSD 0.2 mg/kg, mescaline 100 mg/kg, and DOB 5 mg/kg) on EEG in freely moving rats. A system consisting of 14 cortical EEG electrodes, co-registration of behavioral activity of animals with subsequent analysis only in segments corresponding to behavioral inactivity (resting-state-like EEG) was used in order to reach a high level of translational validity. Analyses of the mean power, topographic brain-mapping, and functional connectivity revealed that all of the psychedelics irrespective of the structural family induced overall and time-dependent global decrease/desynchronization of EEG activity and disconnection within 1–40 Hz. Major changes in activity were localized on the large areas of the frontal and sensorimotor cortex showing some subtle spatial patterns characterizing each substance. A rebound of occipital theta (4–8 Hz) activity was detected at later stages after treatment with mescaline and LSD. Connectivity analyses showed an overall decrease in global connectivity for both the components of cross-spectral and phase-lagged coherence. Since our results show almost identical effects to those known from human EEG/MEG studies, we conclude that our method has robust translational validity.

Potential Neuroprotective Effect of Nanomicelle Curcumin on Learning and Memory Functions Following Subacute Exposure to Bisphenol a in Adult Male Rats

ObjectiveBisphenol A (BPA) is an endocrine-disrupting chemical that widely used in plastics production. It can influence on the brain tissue. Curcumin has a strong protective activity against brain disorders. The purpose of this study was to evaluate the protective effect of nanomicelle curcumin (NmCur) on BPA-induced learning and memory disorders in rats.Material and methodsIn this study, after determining the dose of BPA, rats were randomly divided into 8 groups (8 rats in each group); sesame oil, dextrose 5%, sesame oil + dextrose 5%, NmCur (50 mg/kg), BPA (50 mg/kg), and 50 mg/kg BPA plus 10, 25, or 50 mg/kg NmCur, respectively. All materials administered via gavage. Behavioral tests were estimated by shuttle-box, open-field, and Morris water maze devices. Then, stress oxidative, pro-inflammatory cytokines, oxidative stress-scavenging enzymes levels, as well as expression of MAPK proteins, glutamate receptors, and memory-related proteins were determined in the hippocampus and cortex tissues. ResultsBPA significantly increased expression of ROS, MDA, TNF-α, IL-6, IL-1β, SOD, GST, p-P38, and p-JNK; however, considerably decreased GSH, GPx, GR, CAT, p-AKT, p-ERK1/2 levels. In addition, it down regulated expression of p-NR1, p-NR2A, p-NR2B, p-GluA1, BDNF, and p-CREB in rat cortex and hippocampus tissues. BPA significantly also changed behavioral activity. Conversely, BPA (50 mg/kg) plus NmCur (25 and 50 mg/kg) significantly reversed all BPA-induced adverse effects. ConclusionThe results of this study support that nanomicelle curcumin exhibited preventive effects against neurotoxicity and learning and memory impairment induced by subacute exposure to bisphenol A.

Effect of the Model of one-Time Shift Work in the Highlands on the Behavior and Structural Organization of the Cerebellum

In Kyrgyzstan, 94% of the entire territory of the country is occupied by mountains. There are significant resources located here, the development of which is often associated with the temporary ascent to significant heights and descent to the low mountains of specialists in various fields of work. At the same time, information about the state of brain structures and the behavior of animals and humans in these conditions is scarce. Objective: to study the influence of the model of a one-time 8-hour shift work in the highlands on the histophysiology of the cerebellum and individual behavior of rats. The work was performed on 28 white mongrel male rats weighing 250–310 g. The low-mountain series of the experiment was carried out at an altitude of 760 m above sea level (Bishkek). The high-altitude series was modeled on the Tuya-Ashu pass-3200 m above sea level (Kyrgyzstan). Behavioral activity was evaluated using the Open field method, muscle strength according to the S. V. Speransky method on the 3rd day of the experiment. The microcirculation of the cerebellum was studied microscopically under the Olympus B×40 light microscope (Japan). Statistical processing of the obtained data was carried out in the SPSS 16.0 program. In the highlands, the indicators of the mink reflex increase by 1.43 times, and the level of defecation increases by 8.5 times, which is a marker of increased vegetative activity of the body. Remodeling of cerebellar angioarchitectonics in the highlands is characterized by hypercapillarization, signs of blood stagnation, perivascular edema, enlargement of nucleoli, moderate hyperchromia of neurons of the molecular and granular layers. Thus, in the highlands, changes in behavioral activity are more pronounced than the remodeling of capillaries, neurons and glial elements, which are adaptive in nature.

The opposing Chloride Cotransporters KCC and NKCC control locomotor activity in constant light and during long days

Cation Chloride Cotransporters (CCC’s) regulate intracellular chloride ion concentration ([Cl−]i) within neurons, which can reverse the direction of the neuronal response to the neurotransmitter GABA. Na+ K+ Cl− (NKCC) and K+ Cl− (KCC) cotransporters transport Cl− into or out of the cell, respectively. When NKCC activity dominates, the resulting high [Cl−]i can lead to an excitatory and depolarizing response of the neuron upon GABAA receptor opening, while KCC dominance has the opposite effect. This inhibitory-to-excitatory GABA switch has been linked to seasonal adaption of circadian clock function to changing day length, and its dysregulation is associated with neurodevelopmental disorders such as epilepsy. Constant light normally disrupts circadian clock function and leads to arrhythmic behavior. Here, we demonstrate a function for KCC in regulating Drosophila locomotor activity and GABA responses in circadian clock neurons because alteration of KCC expression in circadian clock neurons elicits rhythmic behavior in constant light. We observed the same effects after downregulation of the Wnk and Fray kinases, which modulate CCC activity in a [Cl−]i-dependent manner. Patch-clamp recordings from clock neurons show that downregulation of KCC results in a more positive GABA reversal potential, while KCC overexpression has the opposite effect. Finally, KCC downregulation represses morning behavioral activity during long photoperiods, while downregulation of NKCC promotes morning activity. In summary, our results support a model in which the regulation of [Cl−]i by a KCC/NKCC/Wnk/Fray feedback loop determines the response of clock neurons to GABA, which is important for adjusting behavioral activity to constant light and long-day conditions.