scholarly journals THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD

1941 ◽  
Vol 73 (2) ◽  
pp. 183-190 ◽  
Author(s):  
Colin M. MacLeod ◽  
Oswald T. Avery
Keyword(s):  
Serum Albumin ◽  
Acute Phase ◽  
Sodium Sulfate ◽  
Serum Proteins ◽  
Tap Water ◽  
Normal Serum ◽  
Reactive Protein ◽  
Acute Infections ◽  
Acute Phase Serum

Methods are described for isolating a protein commonly present in the blood of patients during the acute phase of various infections which, unlike the normal serum proteins, is precipitable by the C polysaccharide of Pneumococcus. The reactive protein is present in the fraction of serum albumin precipitated by either ammonium or sodium sulfate between 50 and 75 per cent saturation. From this fraction the reactive protein separates out on dialysis against tap water. Following removal of the alcohol-ether-soluble lipids from acute phase serum the reactive protein becomes soluble in tap water, and is no longer precipitable by traces of calcium but still retains its precipitability with the C polysaccharide.

scholarly journals THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD

1947 ◽  
Vol 85 (5) ◽  
pp. 491-498 ◽  
Author(s):  
Maclyn McCarty
Keyword(s):  
Infectious Diseases ◽  
Early Phase ◽  
Serum Proteins ◽  
Protein A ◽  
Normal Serum ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Acute Infections

A procedure is described for the isolation and crystallization from human serous fluids of the C-reactive protein, a substance which appears in the blood especially in the early phase of certain acute infectious diseases. Immunological studies confirm earlier work in showing that the protein is highly antigenic and serologically specific, and demonstrate that crystallization of the protein effectively separates it from normal serum proteins.

Serum Glycoproteins and Seromucoid in Patients with Myocardial Infarction

1964 ◽  
Vol 10 (8) ◽  
pp. 736-740
Author(s):  
J W Keyser ◽  
B T Stephens
Keyword(s):  
Myocardial Infarction ◽  
Serum Albumin ◽  
Serum Proteins ◽  
Tissue Injury ◽  
Electrophoretic Analysis ◽  
C Reactive Protein ◽  
Glutamic Oxalacetic Transaminase ◽  
Reactive Protein ◽  
Pas Staining

Abstract Seromucoid and serum total glycoproteins were measured in 7 patients with myocardial infarction. In all cases the highest concentrations of these proteins were reached several days after the concentration of glutamic-oxalacetic transaminase had begun to return to normal. In 2 cases paper electrophoretic analysis of the serum proteins was performed. A rise in the concentration of 1- and 2-globulins occurred after infarction, and PAS staining indicated that the rise in total glycoprotein was largely or entirely accounted for by these two fractions. The concentration of serum albumin fell considerably after infarction, reaching a minimum at about 6 days; - and -globulin concentrations appeared to fall slightly. In general, the presence of C-reactive protein corresponded roughly with the period during which the seromucoid concentration was elevated. It is suggested that the elevation of seromucoid and total glycoproteins represents a nonspecific response to the tissue injury.

scholarly journals AN IMMUNOLOGICAL AND ELECTROPHORETIC COMPARISON OF THE ANTIBODY TO C POLYSACCHARIDE AND THE C-REACTIVE PROTEIN OF ACUTE PHASE SERUM

1943 ◽  
Vol 77 (2) ◽  
pp. 97-110 ◽  
Author(s):  
Ely Perlman ◽  
Jesse G. M. Bullowa ◽  
Ruth Goodkind
Keyword(s):  
Acute Phase ◽  
Gamma Globulin ◽  
Acute Phase Protein ◽  
Precipitation Reaction ◽  
Globulin Fraction ◽  
C Reactive Protein ◽  
C Protein ◽  
Reactive Protein ◽  
Phase Protein ◽  
Acute Phase Serum

1. Studies of the precipitation reaction of C polysaccharide with C protein, and of C polysaccharide with C antibody are reported. The similarity between these two systems in this respect is demonstrated. 2. The differences between C protein and C antibody are emphasized. The differences between this protein and antibodies in general have been reported previously by others. 3. Electrophoretic studies show that C antibody is in the gamma globulin fraction of serum whereas C protein migrates with the alpha1 globulin fraction of acute phase protein.

Increased levels of acute-phase serum proteins in diabetes

Metabolism ◽  
1989 ◽  
Vol 38 (11) ◽  
pp. 1042-1046 ◽  
Author(s):  
Donald E. McMillan
Keyword(s):  
Acute Phase ◽  
Serum Proteins ◽  
Acute Phase Serum

scholarly journals Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients

2018 ◽  
Vol 56 (3) ◽  
pp. 436-440 ◽  
Author(s):  
Sigurd Delanghe ◽  
Wim Van Biesen ◽  
Nadeige Van de Velde ◽  
Sunny Eloot ◽  
Anneleen Pletinck ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Acute Phase ◽  
High Performance ◽  
Acute Phase Proteins ◽  
Hemodialysis Patients ◽  
Uremic Toxins ◽  
Bromocresol Green ◽  
Bromocresol Purple ◽  
Acid Glycoprotein ◽  
Reactive Protein

AbstractBackground:Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin.Methods:Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated.Results:Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V.Conclusions:In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.

scholarly journals THE OCCURRENCE DURING ACUTE INFECTIONS OF A PROTEIN NOT NORMALLY PRESENT IN THE BLOOD

1954 ◽  
Vol 100 (1) ◽  
pp. 71-79 ◽  
Author(s):  
Harrison F. Wood ◽  
Maclyn McCarty ◽  
Robert J. Slater
Keyword(s):  
Isoelectric Point ◽  
Normal Serum ◽  
Globulin Fraction ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Acute Infections ◽  
Zone Electrophoresis

A method is described for obtaining crystalline C-reactive protein from serous fluids in which the protein is associated with lipid. Most pathological fluids currently available as a source of this protein appear to fall in this category. Crystalline C-reactive protein has its isoelectric point at pH 4.82 as determined by free electrophoresis in McIlvaine's buffer. Its mobility in the electrophoresis cell, both alone and after addition to normal serum, coincides with that of the ß-globulin fraction of the serum. In contrast to this finding, by the method of zone electrophoresis on a starch supporting medium the protein migrates with the γ1-globulin. The significance of this discrepancy is discussed. Studies in the ultracentrifuge indicate an s20,w of 7.5.

scholarly journals ELECTROPHORESIS OF PROTEINS ON FILTER PAPER

1951 ◽  
Vol 35 (1) ◽  
pp. 89-118 ◽  
Author(s):  
Henry G. Kunkel ◽  
Arne Tiselius
Keyword(s):  
Serum Albumin ◽  
Filter Paper ◽  
Serum Proteins ◽  
Paper Electrophoresis ◽  
Normal Serum ◽  
Concentration Gradients ◽  
Simplified Procedure ◽  
Highly Porous ◽  
Electrophoresis Of Proteins ◽  
Serum Components

A simplified procedure for filter paper electrophoresis is described in which disturbing factors such as evaporation, heating, buffer concentration gradients, and pH changes in the electrode vessels were reduced to a minimum. Artificial mixtures of highly purified proteins could be separated and the components isolated. The application of the method to a variety of studies on serum proteins is demonstrated. Protein concentration in paper segments was determined by two different methods of protein estimation. Curves were obtained showing the same five major peaks for normal serum as found by the classical methods of free electrophoresis. Comparisons were made of the areas of the various components under the curves obtained with the different methods. Two dimensional electrophoresis was applied to serum and serum components. It proved of value in demonstrating the heterogeneity of fractions such as the γ-globulin of serum. The polysaccharide dextran was used as an index of the extent of electro-osmotic flow during the course of the various experiments. The ratio of the distance of electroosmotic flow and the distance of protein migration was shown to be constant for a given type of paper. For serum albumin on Munktell 20 paper this ratio was 0.35. A formula for mobilities applicable to liquid in a highly porous supporting medium is presented. Mobility values for human serum albumin at various pH levels on paper showed approximate agreement with those obtained in free solution giving a similar isoelectric point.

Ferritin correlates with C-reactive protein and acute phase serum amyloid A in synovial fluid, but not in serum

Amyloid ◽  
1999 ◽  
Vol 6 (2) ◽  
pp. 130-135 ◽  
Author(s):  
Yoshitaka Kumon ◽  
Tadashi Suehiro ◽  
Koji Nishiya ◽  
Kozo Hashimoto ◽  
Ko Nakatani ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Acute Phase ◽  
Serum Amyloid A ◽  
Serum Amyloid ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A ◽  
Acute Phase Serum

scholarly journals STUDIES ON IMMUNITY IN ANTHRAX

1951 ◽  
Vol 93 (2) ◽  
pp. 99-106 ◽  
Author(s):  
George G. Wright ◽  
Janet B. Slein
Keyword(s):  
Acute Phase ◽  
Normal Level ◽  
Marked Decrease ◽  
Normal Serum ◽  
Inhibitory Substance ◽  
Abrupt Decrease ◽  
Human Type ◽  
Anthrax Infection ◽  
Normal Human ◽  
Acute Phase Serum

An abrupt decrease in the titer of serum T-agglutinin frequently occurs during the acute phase of anthrax infection in the rabbit. In partially immunized animals which survive the infection the titer returns to normal during convalescence, but has not been observed to rise significantly above the normal level. The presence of a substance capable of inhibiting T-agglutination may be demonstrated in the sera of many of those animals in which a marked decrease in T-agglutinin has occurred. The polysaccharide and polypeptide antigens of B. anthracis do not possess this activity. Sera containing the T-inhibitory substance usually produce a slow hemolysis of T-erythrocytes and also of normal human type O erythrocytes; hemolysis of erythrocytes of other species is irregular. This reactivity of the sera withstands inactivation at 56°C. but is inhibited by citrate. T-inhibitory and hemolytic activities can frequently be increased by incubation of the acute-phase serum at 37°C. The ability of normal serum to neutralize the T-inhibitory and hemolytic activities of acute-phase serum is not significantly increased after recovery from the infection. The meaning of the results is discussed.

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