scholarly journals Binding of bromocresol green and bromocresol purple to albumin in hemodialysis patients

2018 ◽  
Vol 56 (3) ◽  
pp. 436-440 ◽  
Author(s):  
Sigurd Delanghe ◽  
Wim Van Biesen ◽  
Nadeige Van de Velde ◽  
Sunny Eloot ◽  
Anneleen Pletinck ◽  
...  
Keyword(s):  
Serum Albumin ◽  
Acute Phase ◽  
High Performance ◽  
Acute Phase Proteins ◽  
Hemodialysis Patients ◽  
Uremic Toxins ◽  
Bromocresol Green ◽  
Bromocresol Purple ◽  
Acid Glycoprotein ◽  
Reactive Protein

AbstractBackground:Colorimetric albumin assays based on binding to bromocresol purple (BCP) and bromocresol green (BCG) yield different results in chronic kidney disease. Altered dye binding of carbamylated albumin has been suggested as a cause. In the present study, a detailed analysis was carried out in which uremic toxins, acute phase proteins and Kt/V, a parameter describing hemodialysis efficiency, were compared with colorimetrically assayed (BCP and BCG) serum albumin.Methods:Albumin was assayed using immunonephelometry on a BN II nephelometer and colorimetrically based on, respectively, BCP and BCG on a Modular P analyzer. Uremic toxins were assessed using high-performance liquid chromatography. Acute phase proteins (C-reactive protein and α1-acid glycoprotein) and plasma protein α2-macroglobulin were assayed nephelometrically. In parallel, Kt/V was calculated.Results:Sixty-two serum specimens originating from hemodialysis patients were analyzed. Among the uremic toxins investigated, total para-cresyl sulfate (PCS) showed a significant positive correlation with the BCP/BCG ratio. The serum α1-acid glycoprotein concentration correlated negatively with the BCP/BCG ratio. The BCP/BCG ratio showed also a negative correlation with Kt/V.Conclusions:In renal insufficiency, the BCP/BCG ratio of serum albumin is affected by multiple factors: next to carbamylation, uremic toxins (total PCS) and α1-acid glycoprotein also play a role.

scholarly journals Luminometric assays of seven acute-phase proteins in minimal volumes of serum, plasma, sputum, and bronchioalveolar lavage.

1986 ◽  
Vol 32 (5) ◽  
pp. 743-747 ◽  
Author(s):  
J Braun ◽  
T Schultek ◽  
K F Tegtmeier ◽  
A Florenz ◽  
C Rohde ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Immunoglobulin A ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Coefficients Of Variation ◽  
Alpha 1 Antitrypsin ◽  
Heel Prick ◽  
Secretory Immunoglobulin

Abstract We describe immunoluminometric assays for seven acute-phase proteins, which can be determined in minimal volumes of plasma, serum, sputum, and bronchioalveolar lavage. The theoretical volume of serum or plasma required to measure all seven analytes in duplicate is 130 nL, although in practice the smallest volume of sample was enough to fill a hematocrit tube (about 25 microL of blood), collected from neonates by the heel-prick method. The assays could be performed with 10 microL of sputum or with 100 microL of bronchioalveolar lavage. We measured alpha 1-antitrypsin, alpha 2-macroglobulin, alpha 1-acid glycoprotein, thyroxin-binding prealbumin, C-reactive protein, and total and secretory immunoglobulin A. The assays are rapid enough for all results to be returned to the ward on the same day and are suitable for monitoring neonatal sepsis. All coefficients of variation, derived from compound precision profiles, were less than 7% for clinically relevant analyte concentrations. Correlation with commercially available nephelometric assays was good.

Biological Variation of Acute Phase Proteins

1993 ◽  
Vol 30 (4) ◽  
pp. 373-376 ◽  
Author(s):  
Geraldine H Clark ◽  
Callum G Fraser
Keyword(s):  
Serum Albumin ◽  
Healthy Subjects ◽  
Acute Phase Proteins ◽  
Population Based ◽  
Biological Variation ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Methodological Improvement ◽  
Apparently Healthy

The analytical, within-subject and between-subject components of variation were estimated for serum albumin, transthyretin, α1-acid glycoprotein, α1-antichymotrypsin, haptoglobin, β2-microglobulin and C-reactive protein in a cohort of 19 apparently healthy subjects over 20 weeks. Desirable analytical goals based on biological variation should be able to be met except for serum albumin and β2-microglobulin for which methodological improvement is warranted. All proteins showed marked individuality which casts doubt on the utility of conventional population-based reference values as interpretative criteria. The critical differences required for significance of changes in serial results differ markedly from protein to protein and the data presented allow generation of objective criteria for monitoring individuals.

scholarly journals Impact of Roux-en-Y gastric bypass on lipid and inflammatory profiles

2015 ◽  
Vol 42 (5) ◽  
pp. 305-310 ◽  
Author(s):  
Cleiton da Silva Oliveira ◽  
Bruna Teles Soares Beserra ◽  
Raphael Salles Granato Cunha ◽  
Ana Gabriela Estevam Brito ◽  
Rafaella Cristina Dimbarre de Miranda ◽  
...  
Keyword(s):  
Gastric Bypass ◽  
Total Cholesterol ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Plasma Concentrations ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Significant Difference ◽  
A Prospective Study

Objective: To evaluate the behavior of acute phase proteins and lipid profile in patients undergoing Roux-en-Y gastric bypass. Methods : We conducted a prospective study, consisting of three moments: M1 - preoperative (24 hours before surgery); M2 - 30 days after surgery; and M3 - 180 days after surgery. We carried measured height and BMI, as well as determined the concentrations of acute phase proteins (C-reactive protein (CRP), albumin and Alpha-1-acid glycoprotein) and total cholesterol, LDL-c, HDL-c and triacylglycerol. Results : participants comprised 25 individuals, with a mean age of 39.28 ± 8.07, 72% female. At all times of the study there was statistically significant difference as for weight loss and BMI. We found a significant decrease in CRP concentrations between the moments M1 and M3 (p = 0.041) and between M2 and M3 (p = 0.018). There was decrease in Alpha-1-GA concentrations between M1 and M2 (p = 0.023) and between M1 and M3 (p = 0.028). The albumin values increased, but did not differ between times. Total cholesterol and triacylglycerol decreased significantly ay all times. LDL-c concentrations decreased and differed between M1 and M2 (p = 0.001) and between M1 and M3 (p = 0.001). HDL-c values increased, however only differing between M1 and M2 (p = 0.050). Conclusion : Roux-en-Y gastric bypass promoted a decrease in plasma concentrations of CRP and Alpha-1-acid glycoprotein, improving lipid and inflammatory profiles.

scholarly journals Serum Total Sialic Acid and Lipid-Associated Sialic Acid in Normal Individuals and Patients with Myocardial Infarction, and Their Relationship to Acute Phase Proteins

1993 ◽  
Vol 30 (4) ◽  
pp. 383-386 ◽  
Author(s):  
M Haq ◽  
S Haq ◽  
P Tutt ◽  
M Crook
Keyword(s):  
Myocardial Infarction ◽  
Sialic Acid ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Total Sialic Acid ◽  
Normal Individuals ◽  
The Mean

Serum total sialic acid (TSA) has gained medical interest, particularly as a cardiovascular risk factor and it has been hypothesized that serum levels relate to serum acute phase proteins, some of which are sialylated. We assayed serum TSA and also lipid associated sialic acid (LASA) in SO normal individuals (24 male) and IS subjects (12 male) who had experienced a myocardial infarct. The mean serum TSA in the normal individuals was 2·05 SD 0·38 mmol/L (range 1·16–2·74) and the mean serum LASA was 0.70 SD 0·19 mmol/L (range 0·23–1·03). We also measured five serum acute phase proteins and found a good correlation between these and serum TSA: C-reactive protein, r = 0.52, P<0.001, α-1-antichymotrypsin, r=0·79, P<0·0001, α-2-macroglobulin, r=0·38, P<0·01 and α-1-acid glycoprotein, r=0·32, P<0·05. A significant correlation between plasma TSA and plasma C-reactive protein ( r = 0·47, P<0·04) and also Fibrinogen ( r=0·53, P<0·04) was noted on day one following the myocardial infarction, whereas a significant correlation between plasma TSA and plasma α-1-antichymotrypsin ( r=0·51, P<0·03) and also plasma α-1-acid glycoprotein ( r=0·64, P<0·05) was found on day two following the infarction. Thus it would seem that serum TSA is at least in part related to some of the acute phase proteins in both healthy individuals and those having had a myocardial infarction.

scholarly journals The Acute Phase Proteins Reaction in Children Suffering from Pseudocroup

2019 ◽  
Vol 2019 ◽  
pp. 1-7
Author(s):  
Beata Pucher ◽  
Magdalena Sobieska ◽  
Michal Grzegorowski ◽  
Jaroslaw Szydlowski
Keyword(s):  
Acute Phase ◽  
Inflammatory Reaction ◽  
Acute Phase Proteins ◽  
Control Group ◽  
C Reactive Protein ◽  
Acid Glycoprotein ◽  
Reactive Protein ◽  
Persistent Inflammation ◽  
Alpha 1 Antitrypsin

The aim of the study was to evaluate the inflammatory reaction in children with pseudocroup and compare it with other laryngological diseases according to the available literature data. The study group included 51 children hospitalized because of pseudocroup. The measurements of the acute phase proteins (APP), such as C-reactive protein (CRP), alpha-1-antitrypsin (AT), alpha-1-antichymotrypsin (ACT), alpha-1-acid glycoprotein (AGP), ceruloplasmin (Cp), transferrin (Tf), alpha-2-macroglobulin (A2M), and haptoglobin (Hp) were obtained at 3 time points. The glycosylation profiles of AGP, ACT, and Tf were completed. An increased AGP level was observed in girls. The AGP glycosylation revealed the advantage of the W0 variant over the W1 variant. W1 and W2 were decreased in boys. W3 emerged in boys. The Tf concentration and T4 variant were lower compared to the control group. The A2M level was lower after treatment. The Hp and AT levels were decreased a few weeks later. The ACT glycosylation revealed a decrease of the A4 variant in boys. In conclusion, the inflammatory reaction during pseudocroup was of low intensity. The APP glycosylation suggested a chronic process. In a follow-up investigation, no normalization of the parameters was noted, but signs of persistent inflammation were observed.

Variations in the levels of acute-phase proteins and lactoferrin in serum and milk during bovine subclinical mastitis

2021 ◽  
pp. 1-5
Author(s):  
Aarsha Raj ◽  
Vinodkumar Kulangara ◽  
Tresamol P. Vareed ◽  
Deepa P. Melepat ◽  
Latha Chattothayil ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Solid Phase ◽  
Bovine Mastitis ◽  
Subclinical Mastitis ◽  
Clinical Mastitis ◽  
Reactive Protein ◽  
Amyloid A ◽  
California Mastitis Test ◽  
Bacterial Organism

Abstract Variations in the levels of acute phase proteins and lactoferrin in serum and milk for diagnosis of subclinical mastitis in dairy cows are described in this research paper. Milking animals from two organized dairy farms in Kerala, India, were screened by California Mastitis Test (CMT), Electrical Conductivity test (EC) and Somatic Cell Count (SCC) test to identify animals affected with sub clinical mastitis (SCM). The concentrations of acute phase proteins (APP) Haptoglobin (Hp), C- reactive protein (CRP), Albumin, Lactoferrin (Lf) and α- 1 acid glycoprotein (AGP) in milk and Hp, Albumin, Serum Amyloid A (SAA) and CRP in the serum of 40 normal cows and 40 cows affected with sub clinical mastitis were assessed. Solid phase ELISA was employed for assessment of all parameters except the albumin levels, for which spectrophotometry was used. The values of Hp in milk; and SAA, AGP and Lf in serum, were significantly elevated in the group with sub clinical mastitis. Such variations were found to be independent of the specific bacterial organism causing the disease. These results show that significant variations exist in the levels of acute phase proteins Hp, AGP and Lf in milk, and SAA in serum of animals affected with subclinical bovine mastitis that are not affected by specific bacterial etiology.

scholarly journals Experimental Ehrlichia canis infection changes acute-phase proteins

2012 ◽  
Vol 21 (3) ◽  
pp. 206-212 ◽  
Author(s):  
Thiago Demarchi Munhoz ◽  
Joice Lara Maia Faria ◽  
Giovanni Vargas-Hérnandez ◽  
José Jurandir Fagliari ◽  
Áureo Evangelista Santana ◽  
...  
Keyword(s):  
Early Detection ◽  
Early Diagnosis ◽  
Acute Phase ◽  
Acute Phase Proteins ◽  
Ehrlichia Canis ◽  
Laboratory Findings ◽  
Early Detection Of Disease ◽  
Acid Glycoprotein ◽  
The Third ◽  
Canine Ehrlichiosis

Early diagnosis of canine ehrlichiosis favors prompt institution of treatment and improves the prognosis for the animal, since this disease causes mortality among dogs. Studies have shown that determining the concentration of acute-phase proteins (APPs) may contribute towards early detection of disease and aid in predicting the prognosis. This study aimed to evaluate the APP profile in dogs experimentally infected with Ehrlichia canis, at the start of the infection and after treatment. It also investigated whether any correlation between APP levels and the clinical and laboratory alterations over the course of the disease would be possible. The results obtained showed abnormal levels of all the APPs on the third day after infection (D3), with the highest levels being reached on D18, with the exception of ceruloplasmin and acid glycoprotein, which presented their peaks on D6 and D12 respectively. We concluded that assessment of APP levels could contribute towards establishing an early diagnosis of canine ehrlichiosis, particularly regarding acid glycoprotein and ceruloplasmin, since these proteins were detected at increased levels even before the onset of clinical and laboratory findings of the disease.

scholarly journals Evaluation of Plasmatic Levels of Negative and Positive Acute Hepatic Phase Proteins in Patients who Underwent Major Surgery

2019 ◽  
Vol 70 (11) ◽  
pp. 3947-3953
Author(s):  
Laura Nicolescu ◽  
Bogdan Totolici ◽  
Ovidiu Bedreag ◽  
Cristian Nicolescu ◽  
Alin Mihu
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Major Surgery ◽  
P Value ◽  
Time Intervals ◽  
Reactive Protein ◽  
Pearson Coefficient ◽  
Simultaneous Decrease ◽  
Correlation Tests ◽  
The Moment

The acute hepatic phase response is defined as a reaction that includes hepatic synthesis of proteins, consisting in the increase of some proteins called positive acute phase proteins and the simultaneous decrease of others called negative acute phase proteins. This study describes this hepatic reaction, based on a series of consecutive determinations, at three different time intervals (right before a major surgery event, 24 and 48 hours after the intervention), of the plasmatic levels of transferrin, albumin, fibrinogen and C reactive protein (CRP). Subsequently, the data was analyzed using Jamovi 2019, version 0.9. The inferential statistics consisted in calculating an ANOVA test that compared the values at 24 hours and 48 hours versus the values right before the major surgery. The results were validated by calculating the p value (p[0.05) as well as conducting correlation tests by determining the Pearson coefficient which shows the values of CRP, fibrinogen, transferrin and albumin are independent of each other and do not interact. The multiple ANOVA comparative test reveals the lack of interaction between the values of determined proteins, regardless of the moment of determination.

The acute phase response and C-reactive protein

2020 ◽  
pp. 2199-2207
Author(s):  
Mark B. Pepys
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Serum Amyloid A ◽  
Acute Phase Proteins ◽  
Phase Response ◽  
Serum Amyloid ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Amyloid A ◽  
Serum Amyloid A Protein

The acute phase response—trauma, tissue necrosis, infection, inflammation, and malignant neoplasia induce a complex series of nonspecific systemic, physiological, and metabolic responses including fever, leucocytosis, catabolism of muscle proteins, greatly increased de novo synthesis and secretion of a number of ‘acute phase’ plasma proteins, and decreased synthesis of albumin, transthyretin, and high- and low-density lipoproteins. The altered plasma protein concentration profile is called the acute phase response. Acute phase proteins—these are mostly synthesized by hepatocytes, in which transcription is controlled by cytokines including interleukin 1, interleukin 6, and tumour necrosis factor. The circulating concentrations of complement proteins and clotting factors increase by up to 50 to 100%; some of the proteinase inhibitors and α‎1-acid glycoprotein can increase three- to fivefold; but C-reactive protein (CRP) and serum amyloid A protein (an apolipoprotein of high-density lipoprotein particles) are unique in that their concentrations can change by more than 1000-fold. C-reactive protein—this consists of five identical, nonglycosylated, noncovalently associated polypeptide subunits. It binds to autologous and extrinsic materials which contain phosphocholine, including bacteria and their products. Ligand-bound CRP activates the classical complement pathway and triggers the inflammatory and opsonizing activities of the complement system, thereby contributing to innate host resistance to pneumococci and probably to recognition and safe ‘scavenging’ of cellular debris. Clinical features—(1) determination of CRP in serum or plasma is the most useful marker of the acute phase response in most inflammatory and tissue damaging conditions. (2) Acute phase proteins may be harmful in some circumstances. Sustained increased production of serum amyloid A protein can lead to the deposition of AA-type, reactive systemic amyloid.

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