Human breast cancerin vitro: matching histo-pathology with small-angle x-ray scattering and diffraction enhanced x-ray imaging

2005 ◽  
Vol 50 (13) ◽  
pp. 2991-3006 ◽  
Author(s):  
Manuel Fernández ◽  
Jani Keyriläinen ◽  
Ritva Serimaa ◽  
Mika Torkkeli ◽  
Marja-Liisa Karjalainen-Lindsberg ◽  
...  
2004 ◽  
Vol 37 (5) ◽  
pp. 757-765 ◽  
Author(s):  
L. E. Levine ◽  
G. G. Long

A new transmission X-ray imaging technique using ultra-small-angle X-ray scattering (USAXS) as a contrast mechanism is described. USAXS imaging can sometimes provide contrast in cases where radiography and phase-contrast imaging are unsuccessful. Images produced at different scattering vectors highlight different microstructural features within the same sample volume. When used in conjunction with USAXS scans, USAXS imaging provides substantial quantitative and qualitative three-dimensional information on the sizes, shapes and spatial arrangements of the scattering objects. The imaging technique is demonstrated on metal and biological samples.


2008 ◽  
Vol 41 (2) ◽  
pp. 416-427 ◽  
Author(s):  
F. Zhang ◽  
G. G. Long ◽  
L. E. Levine ◽  
J. Ilavsky ◽  
P. R. Jemian

A general treatment of X-ray imaging contrast for ultra-small-angle X-ray scattering (USAXS) imaging is presented; this approach makes use of phase propagation and dynamical diffraction theory to account quantitatively for the intensity distribution at the detector plane. Simulated results from a model system of micrometre-sized spherical SiO2particles embedded in a polypropylene matrix show good agreement with experimental measurements. Simulations by means of a separate geometrical ray-tracing method also account for the features in the USAXS images and offer a complementary view of small-angle X-ray scattering as a contrast mechanism. The ray-tracing analysis indicates that refraction, in the form of Porod scattering, and, to a much lesser extent, X-ray reflection account for the USAXS imaging contrast.


2002 ◽  
Vol 47 (4) ◽  
pp. 577-592 ◽  
Author(s):  
M Fernández ◽  
J Keyriläinen ◽  
R Serimaa ◽  
M Torkkeli ◽  
M-L Karjalainen-Lindsberg ◽  
...  

2014 ◽  
Vol 517 ◽  
pp. 012017 ◽  
Author(s):  
Y Hayakawa ◽  
K Hayakawa ◽  
M Inagaki ◽  
T Kaneda ◽  
K Nakao ◽  
...  

2012 ◽  
Vol 1 (1) ◽  
Author(s):  
Martin Bech ◽  
Simone Schleede ◽  
Guillaume Potdevin ◽  
Klaus Achterhold ◽  
Oliver Bunk ◽  
...  

AbstractX-ray phase and dark-field contrast have recently been the source of much attention in the field of X-ray imaging, as they both contribute new imaging signals based on physical principles that differ from conventional X-ray imaging. With a so-called Talbot grating interferometer, both phase-contrast and dark-field images are obtained simultaneously with the conventional attenuation-based X-ray image, providing three complementary image modalities that are intrinsically registered. Whereas the physical contrast mechanisms behind attenuation and phase contrast are well understood, a formalism to describe the dark-field signal is still in progress. In this article, we report on correlative experimental results obtained with a grating interferometer and with small-angle X-ray scattering. Furthermore, we use a proposed model to quantitatively describe the results, which could be of great importance for future clinical and biomedical applications of grating-based X-ray imaging.


2017 ◽  
Vol 44 ◽  
pp. 236-242 ◽  
Author(s):  
Daisuke Shimao ◽  
Naoki Sunaguchi ◽  
Tenta Sasaya ◽  
Tetsuya Yuasa ◽  
Shu Ichihara ◽  
...  

2004 ◽  
Vol 18 (2) ◽  
pp. 167-176 ◽  
Author(s):  
M. Fernández ◽  
J. Keyriläinen ◽  
M.-L. Karjalainen-Lindsberg ◽  
M. Leidenius ◽  
K. von Smitten ◽  
...  

Small-angle X-ray scattering (SAXS) patterns from breast tissue samples are compared with their histology. Formalin fixed human breast tissue specimens containing ductal and lobular carcinoma were studied. Histo-pathological information is compared with the scattering data, and there is a clear spatial correlation. Supra-molecular organisation of collagen fibrils is modelled and the model is used to create scattering maps. The model parameters include the axial periodicity (d-spacing), radius and packing of the fibrils, and these are derived from comparison with the experimental scattering patterns. Thed-spacing is to 0.5% larger in malignant zones of the tumours than in the healthy zones. There are also characteristic differences in the fibril diameter and packing.


2019 ◽  
Author(s):  
Christian Prehal ◽  
Aleksej Samojlov ◽  
Manfred Nachtnebel ◽  
Manfred Kriechbaum ◽  
Heinz Amenitsch ◽  
...  

<b>Here we use in situ small and wide angle X-ray scattering to elucidate unexpected mechanistic insights of the O2 reduction mechanism in Li-O2 batteries.<br></b>


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