Cytotoxicity of hyaluronic acid coated chitosan nanoparticles containing nitric oxide donor against cancer cell lines

Clinacanthus nutansLindau leaves (CN) have been used in traditional medicine but the therapeutic potential has not been explored for cancer prevention and treatment. Current study aimed to evaluate the antioxidant and antiproliferative effects of CN, extracted in chloroform, methanol, and water, on cancer cell lines. Antioxidant properties of CN were evaluated using DPPH, galvinoxyl, nitric oxide, and hydrogen peroxide based radical scavenging assays, whereas the tumoricidal effect was tested on HepG2, IMR32, NCL-H23, SNU-1, Hela, LS-174T, K562, Raji, and IMR32 cancer cells using MTT assay. Our data showed that CN in chloroform extract was a good antioxidant against DPPH and galvinoxyl radicals, but less effective in negating nitric oxide and hydrogen peroxide radicals. Chloroform extract exerted the highest antiproliferative effect on K-562 (91.28±0.03%) and Raji cell lines (88.97±1.07%) at 100 μg/ml and the other five cancer cell lines in a concentration-dependent manner, but not on IMR-32 cells. Fourteen known compounds were identified in chloroform extract, which was analysed by gas chromatography—mass spectra analysis. In conclusion, CN extracts possess antioxidant and antiproliferative properties against cultured cancer cell lines, suggesting an alternate adjunctive regimen for cancer prevention or treatment.

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The adipokine vaspin reduces apoptosis in human hepatocellular carcinoma (Hep-3B) cells, associated with lower levels of NO and superoxide anion

BMC Pharmacology and Toxicology ◽

10.1186/s40360-019-0334-6 ◽

2019 ◽

Vol 20 (1) ◽

Cited By ~ 1

Author(s):

Magdalena Skonieczna ◽

Dorota Hudy ◽

Tomasz Hejmo ◽

Rafal J. Buldak ◽

Małgorzata Adamiec ◽

...

Keyword(s):

Nitric Oxide ◽

Hepatocellular Carcinoma ◽

Cell Lines ◽

Cancer Cell ◽

Superoxide Anion ◽

Reactive Oxygen ◽

Cancer Cell Lines ◽

Protective Role ◽

Apoptotic Pathway ◽

Physiological Level

Abstract Background Among adipose-derived factors, adipocytokines play roles as hormones and signaling mediators for apoptotic pathway. Among of them, vaspin, regulates the metabolism of adipose tissue itself as an endocrine organ, and stimulates adipocytes to maturation, differentiation, etc. Damaged adipocytes, present in obesity and hepatocellular carcinoma (HCC) respond with over-production of inflammatory cytokines. Such pro-inflammatory stimulation remains under adipokine control. Pro-inflammatory pathways are connected to oxidative stress and apoptosis, reported as co-existing with an elevated level of some adipokines in cancer cell lines. However, some hormones, such as vaspin, reduce apoptosis, have anti-inflammatory and anti-oxidative roles in cancer cell lines. Methods Hep-3B cells were cytometrically evaluated under vaspin treatment for reactive oxygen species (ROS) and apoptosiss induction. The statistical significant changes to the untreated controls was calculated by T-tests (indicated at value p < 0.05). Results Here we studied the production of reactive oxygen and nitrogen species in cells of HCC line Hep-3B after vaspin treatment. A decreased level of nitric oxide and superoxide anion 24 h after vaspin addition at 5 ng/ml was correlated with restricted, to the physiological level, apoptosis. A protective role of vaspin was displayed as enhanced cell viability and proliferation, which could be a poor prognostic in liver cancer. Conclusions Apoptosis was suppressed after vaspin treatment, together with low levels of nitric oxide and superoxide anions.

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APOPTOTIC AND NECROTIC EFFECTS OF CHITOSAN NANOPARTICLES LOADED WITH THE HONEYBEE, APIS MELLIFERA VENOM ON DIFFERENT CANCER CELL LINES

Journal of the Egyptian Society of Parasitology ◽

10.21608/jesp.2019.68293 ◽

2019 ◽

Vol 49 (1) ◽

pp. 115-122 ◽

Cited By ~ 1

Author(s):

SAMAA EL-DEK ◽

MOSTAFA HASSAN ◽

ALY MOHAMED ◽

ABDEL WAHAB ABDEL WAHAB

Keyword(s):

Apis Mellifera ◽

Cell Lines ◽

Cancer Cell ◽

Chitosan Nanoparticles ◽

Cancer Cell Lines

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CHITOSAN NANOPARTICLES MEDIATED DELIVERY OF MIR-106B-5P TO BREAST CANCER CELL LINES MCF-7 AND T47D

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2021v13i1.39749 ◽

2021 ◽

pp. 129-134

Author(s):

LEONNY DWI RIZKITA ◽

YSRAFIL ◽

RONNY MARTIEN ◽

INDWIANI ASTUTI

Keyword(s):

Breast Cancer ◽

Cell Lines ◽

Cancer Cell ◽

Breast Cancer Cell ◽

Chitosan Nanoparticles ◽

Cancer Cell Lines ◽

Breast Cancer Cell Lines ◽

Size Analysis ◽

Control Group ◽

Mcf 7

Objective: The development of nanomedicine, such as miRNA transfection to cancer cells,has widely gained interest in the past decade. Unfortunately, miRNA tends to decay easily by the cellular enzymatic process and requires a carrier. As a cationic biopolymer, chitosan is widely known as a non-viral vector. However, research about chitosan as a miRNA delivery system remains limited. This study aimed to investigate the effect and characters of synthetic miRNA loaded chitosan nanoparticles on breast cancer cell lines. Methods: To obtain the nanocomplex, chitosan-antimiR-106b-5p was formulated using natriumtripolyphosphate through ionic gelation methods. The nanochitosan formula was characterized by using gel electrophoresis; Nano Quant for encapsulation of entrapment quantification; morphology appearance as viewed by Scanning Electron Microscope (SEM), nanochitosan size analysis; in vitro analysis using MCF-7 and T47D breast cancer cell lines; in silico prediction of possible gene target; polymerase chain reaction analysis and gel electrophoresis for E2F1/GAPDH expression. Results: The efficiency entrapment value was 96.7%, particle size analysis was 458±11.79 nm, and polydispersity index (PDI) was 0.65±0.07, with spherical morphology as viewed in SEM. There was no significant difference between the nanochitosan supplemented group and the control group in MCF-7 cells (p=0.067). However, the ratio of E2F1 to GAPDH was significantly lower than the control group after nanochitosan antimiR-106b-5p was loaded at concentration 140 nmol (p=0.022) and 35 nmol (p=0.016). Conclusion: Our nanochitosan formula is non-toxic to use in MCF-7 cell lines. Most importantly, as the formula was conjugated to synthetic antimiR-106b-5p, the E2F1 expression decreased.

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