scholarly journals Characterization of Sound Spectrum based on Natural Animals as an Alternative Source of Harmonic System Audio Bio Stimulators for Increasing Productivity of Food Plants

2019 ◽  
Vol 1387 ◽  
pp. 012098
Author(s):  
Nur Kadarisman ◽  
Dyah Kurniawati Agustika ◽  
Agus Purwanto ◽  
Vinna Alvianty ◽  
Bagoes Wibowo
Keyword(s):  
Food Plants ◽  
Alternative Source ◽  
Sound Spectrum ◽  
Harmonic System

SBA-15 Molecular Sieve Using Clay as Silicon Sources

2014 ◽  
Vol 798-799 ◽  
pp. 116-120 ◽  
Author(s):  
Gustavo Medeiros de Paula ◽  
L.A. Lima ◽  
Meiry Glaúcia Freire Rodrigues
Keyword(s):  
Surface Area ◽  
Great Reduction ◽  
Molecular Sieve ◽  
Total Pore Volume ◽  
Synthesis And Characterization ◽  
Alternative Source ◽  
Research Teams ◽  
Silica Source ◽  
Silicon Sources

New sources of silica as a replacement for TEOS in the synthesis of SBA-15 are been performed by various research teams worldwide. The aim of this work is to describe the synthesis and characterization of SBA-15 made with chocolate B clay as an alternative source of silica by the hydrothermal method. Samples of classic SBA-15 were made with the traditional TEOS method. The samples of the two silica sources were characterized by DRX, EDX, and BET to evaluate the effects of the silica source on the synthesis of the molecular sieve. The chocolate B clay was efficient in the formation of the molecular sieve structure, but there was a great reduction in the surface area and total pore volume in comparison to the surface area of classic SBA-15.

300 ESTABLISHMENT AND CHARACTERIZATION OF RAT MESENCHYMAL STEM CELLS

2011 ◽  
Vol 23 (1) ◽  
pp. 247
Author(s):  
T. H. Kim ◽  
B. G. Jeon ◽  
S. L. Lee ◽  
G. J. Rho
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Transcriptional Factors ◽  
Skin Tissue ◽  
Rt Pcr ◽  
Alternative Source ◽  
Differentiated Cells ◽  
Fetal Bovine

Mesenchymal stem cells (MSC) are regarded as an attractive source for tissue engineering and regeneration, and bone marrow extract has been commonly used as a source of pluripotent MSC. However, skin tissue has recently been identified as a convenient alternative source of MSC. The present study was focused on the effect of characterised MSC derived from rat on expression of early transcriptional factors, alkaline phosphate (AP) activity, and in vitro differentiation into selected cell lineages. The MSC were isolated from 8-week-old s.d. rat’s ear skin and cultured in advanced DMEM supplemented with 10% fetal bovine serum at 37°C in a humidified atmosphere of 5% CO2 in air. To evaluate AP activity, cells were fixed with 3.7% formaldehyde solution and stained with Western Blue® (Promega, Madison, WI, USA). Expressions of early transcriptional factors (Oct-4, Sox2, and Nanog) were evaluated by RT-PCR. Differentiation into distinct mesenchymal lineages such as adipogenic, osteogenic, and neuron was done by following previously described protocols and assessed by lineage-specific stains. The specific genes in the osteocytes (osteocalcin, osteonectin, osteopontin, and Runx2), adipocytes (pparγ2, adiponectin, and aP2) or neuron (nestin, neurogenin 1, β-tublin, and nerve growth factor) were characterised by RT-PCR. The MSC were positive for AP activity and expressed Oct-4, Sox2, and Nanog. Following induction, MSC were successfully differentiated into adipocytes, osteocytes, and neurons. As adipocytes markers, aP2, pparγ2, and adiponectin were strongly detected in the adipocyte induced cells. Osteonectin, osteocalcin, Runx2, and osteopontin were expressed in the adipocyte induced cells. Futhermore, neuron-specific markers were clearly expressed in the neuronal differentiated cells. In conclusion, MSC have the capability of differentiation into multilineages including adipocytes, osteocytes, and neurons under the specific induction conditions. Skin tissue in rat can serve as an easily accessible and expandable alternative source for MSC harvesting and preclinical applications using an animal model. This work was supported by Grant No. 2007031034040 from Bio-organ and 200908FHT010204005 from Biogreen21, Republic of Korea.

scholarly journals Extraction and Characterization of Native Starch From Black and Red Rice

2020 ◽  
Vol 8 (3) ◽  
pp. 1
Author(s):  
Henrique Valentim Moura ◽  
Rennan Pereira de Gusmão ◽  
Thaisa Abrantes Souza Gusmão ◽  
Deise Souza de Castro ◽  
Raphael Lucas Jacinto Almeida ◽  
...  
Keyword(s):  
Lower Cost ◽  
Extraction Yield ◽  
Rice Starch ◽  
Sodium Metabisulfite ◽  
Red Rice ◽  
Black Rice ◽  
Alternative Source ◽  
Rice Grains ◽  
Starch Gels

The objective of the study is to extract and characterize red rice starch, a grain commonly consumed in the Northeast region of Brazil and to compare it with the starch obtained from black rice, widely spread in eastern countries. Starch was extracted by immersion of rice in sodium metabisulfite (0.2%), followed by milling, filtration, decantation and drying at 50 °C. The obtained starches were characterized by the following parameters: physicochemical, physical, rheological, textural, structural, thermal and morphological. The highest starch extraction yield was found in red rice grains (67.84%), where it obtained higher water absorption and lower apparent viscosity, firmness and gum. Both starch gels indicate the behavior of non-Newtonian fluid in the specific case of pseudoplastic crystallinity type A and with polyhedral geometry. For the thermal analysis of red rice starch, it was observed higher gelatinization temperature and lower gelatinization enthalpy. Black and red rice grains can be considered an alternative source of starch, especially red rice, which has a lower cost due to its production in the country.

Synthesis and Characterization of ZSM-5 Zeolite Using Kaolin as Si and Al Source

2014 ◽  
Vol 805 ◽  
pp. 651-656 ◽  
Author(s):  
Valmir José da Silva ◽  
Meiry Glaúcia Freire Rodrigues
Keyword(s):  
Hydrothermal Treatment ◽  
Crystalline Material ◽  
Synthesis And Characterization ◽  
Mass Ratio ◽  
Alternative Source ◽  
Shape Characteristic ◽  
Autogenous Pressure ◽  
Calcined Kaolin

The objective of this work is to obtain synthesis of ZSM-5 zeolite using kaolin as Si and Al alternative source. Kaolin powder was dispersed under constant stirring into the sílica sol where the mass ratio of kaolin to silica was 1:2. After stirring for 2 hours, the dispersion was dried at 160 °C and calcined at 700 °C/2 hours. The synthesis of ZSM-5 zeolite was by hydrothermal treatment at 170 °C/48 hours under autogenous pressure. The materials were characterized by XRD, SEM, EDX, BET. According to the XRD, was possible to observe the formation of ZSM-5 zeolite with intense and well defined peaks set located between 2θ = 7-9° and 23-25°, typical of a crystalline material. The SEM showed the formation of particles clusters with orthorhombic shape characteristic of MFI structure. The results show that the hydrothermal treatment was efficient to produce ZSM-5 using calcined kaolin as alternative source.

Extraction and Partial Characterization of Pepsin-Soluble Collagens from the Skin of Amiurus Nebulosus

2011 ◽  
Vol 236-238 ◽  
pp. 2926-2934 ◽  
Author(s):  
Li Li Chen ◽  
Li Zhao ◽  
Hua Liu ◽  
Run Feng Wu
Keyword(s):  
Type I Collagen ◽  
Type I ◽  
Alternative Source ◽  
Soluble Collagen ◽  
Acid Soluble Collagen ◽  
Sds Page ◽  
Skin Collagen ◽  
Maximal Yield ◽  
Pepsin Soluble Collagen

Pepsin-soluble collagen (PSC) was successfully extracted from the skin of Amiurus nebulosus. The skin of Amiurus nebulosus was immersed in 0.3 mol/L acetic acid (1: 20, m: V) for 6 h at 37°C, while pepsin was added, at a level of 5000U/g dosage of defatted skin. The maximal yield of the collagen was 97.44%, which was higher than that of acid-soluble collagen (ASC) at 62.05%. Some properties of pepsin-soluble collagens from the skin of Amiurus nebulosus were characterized. Amino acid composition and SDS-PAGE suggested that the collagen might be classified as type I collagen. Moreover, FTIR investigations showed the existence of helical arrangements in PSC of Amiurus nebulosus skin of collagen. There is a possibility to use Amiurus nebulosus skin collagen as an alternative source of collagen for industrial purposes and subsequently it may maximize the economical value of the fish.

scholarly journals Extraction and Physicochemical Characterization of Chitin Derived from the Asian Hornet, Vespa velutina Lepeletier 1836 (Hym.: Vespidae)

2019 ◽  
Author(s):  
Xesús Feás ◽  
M. Pilar Vázquez-Tato ◽  
Julio A. Seijas Vázquez ◽  
Francisco Fraga-López
Keyword(s):  
Solid State ◽  
Model Fitting ◽  
Physicochemical Characterization ◽  
Frequency Factor ◽  
Alternative Source ◽  
Promising Alternative ◽  
Vespa Velutina ◽  
Model Free ◽  
Isolation And Characterization

The isolation and characterization of chitin (CHI) obtained from Vespa velutina (CHIVV) is described. Moreover, a trapping procedure is presented to selectively catch the invasive species. The chitin contents of dry Vespa velutina was observed to be 11.7 %. The physicochemical properties of CHIVV was characterized by Fourier transform infrared spectroscopy (FTIR), solid-state NMR (ssNMR), elemental analysis (EA), scanning electron microscopy (SEM) and thermogravimetric analysis (TG). Obtained CHIVV is close to pure (43, 47% C, 6.94% H, and 6.85% N) and full acetylated with a value of 95.44%. Also, lifetime and kinetic parameters such as activation E and the frequency factor A using model-free and model-fitting methods, were determined. For CHIVV the solid state mechanism that follows the thermodegradation is of type F2 (Random nucleation around two nuclei). Vespa velutina chitin should not be used at temperatures above 60ºC, since its half-life would be only one year, and from an industrial point of view it would not be profitable. Based on certain factors such as the current and probable continued abundance of Vespa velutina and the quality of the product obtained, the invasive Asian hornet is a promising alternative source of chitin.

scholarly journals Isolation and characterization of acid and pepsin soluble collagen extracted from sharpnose stingray (Dasyatis zugei) skin

Food Research ◽  
2020 ◽  
Vol 5 (3) ◽  
Author(s):  
T.Y. Ong ◽  
M.I. Shaik ◽  
N.M. Sarbon
Keyword(s):  
Helical Structure ◽  
Alternative Source ◽  
Soluble Collagen ◽  
Isolation And Characterization ◽  
Fibrous Network ◽  
Acid Soluble Collagen ◽  
Maximum Temperatures ◽  
Surface Morphologies ◽  
Pepsin Soluble Collagen

This study aimed to isolate and characterize the acid soluble collagen (ASC) and pepsin soluble collagen (PSC) from the skin of the sharpnose stingray (Dasyatis zugei). Isolated ASC and PSC were subjected to chemical and physical characterizations. The yield of PSC (34.84±1.26%) was significantly higher than that of ASC (20.48±4.41%) (p<0.05). There were no significant differences between ASC and PSC in terms of chemical composition (p>0.05). Both ASC and PSC were thermally stable at high temperatures, with denaturation temperatures of 24.1°C and 25.2°C, respectively, and maximum temperatures of 31.94±0.13°C and 31.79±0.23°C, respectively. Fourier transform infrared (FTIR) investigations showed the presence of triple helical structure with strong hydrogen bonding in both ASC and PSC. Meanwhile, both collagens were highly solubilized at acidic pH but at different optimal pH. The surface morphologies of ASC and PSC were loose and possessed slender, less uniform and irregular fibrous network structures with large and irregular pores observed between the fibrils. This finding showed that the alternative source of marine collagen possesses good physicochemical properties which highly potential for nutraceutical, pharmaceutical or cosmeceutical application.

Isolation and Characterization of Extracellular Enzyme (Glutaminase and Urease) producing Bacteria isolated from Soil Samples of Different Regions of Gwalior, Madhya Pradesh, India

2021 ◽  
Vol 16 (7) ◽  
pp. 122-129
Author(s):  
Neha Sharma ◽  
Shuchi Kaushik ◽  
Rajesh Singh Tomar
Keyword(s):  
Extracellular Enzyme ◽  
Soil Samples ◽  
Dilution Method ◽  
Bacterial Isolates ◽  
Alternative Source ◽  
Isolation And Characterization ◽  
Microbiological Laboratory ◽  
Biochemical Testing ◽  
Industrial Level

Microbial glutaminase and urease have demonstrated their benefits in various fields like medicinal, pharmaceutical and biotechnological industries. Keeping this viewpoint, the aim of the present study was the isolation and characterization of extracellular enzyme-producing bacteria from soil samples collected from different regions of Gwalior (M.P.). The isolated bacterial cultures were processed by serial dilution method and maintained on nutrient agar medium following standard microbiological laboratory practices for maintenance and preservation of bacteria. We screened out three enzyme producing strains of Salmonella sp., Proteus vulgaris and Bacillus subtilis. The screening was based on biochemical testing and enzyme assays. To accomplish this work, we used differential as well as selective media. All the selected isolates were able to produce enzymes like L-Glutaminase and Urease with different specific enzymatic activity. These bacterial isolates were not reported to show any type of allergenicity when their sequences were checked by bioinformatics tool Algpred. So, these bacterial isolates can be considered as an alternative source for the production of enzymes and can be used for largescale production of enzymes at the industrial level.

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