scholarly journals Screening of a pyrene-degrading bacterium and its degradation effect on polycyclic aromatic hydrocarbons oxidation

Author(s):  
Xiaoxia Sang ◽  
Changwei An ◽  
Danfeng Zhang ◽  
Tong Liu ◽  
Yujuan Wen
2014 ◽  
Vol 955-959 ◽  
pp. 302-305
Author(s):  
Xin Xin Wang ◽  
Chen Li ◽  
Li Bin Zhao ◽  
Liang Wu ◽  
Wei An ◽  
...  

The genomes ofAdvenellahave been sequenced to date, which are important bacteria in environment. However, the genome of a single strain can only provide limited information on genetic diversity. Here, genomes ofAdvenellawere compared by bioinformatics methods. A total of 2438 genes were shared by 3 strains ofAdvenella. Amino acid transport and metabolism related genes dominated the shared genes ofAdvenella, indicates the importance of these genes to the survival ofAdvenella. Genes encoding protocatechuate 3,4-dioxygenase was highly conserved in the genusAdvenella. This comparative analysis presents a foundation for future understanding of the biodiversity ofAdvenella.


2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Tony Gutierrez ◽  
William B. Whitman ◽  
Marcel Huntemann ◽  
Alex Copeland ◽  
Amy Chen ◽  
...  

Arenibacter algicola strain TG409 was isolated from Skeletonema costatum and exhibits the ability to utilize polycyclic aromatic hydrocarbons as sole sources of carbon and energy. Here, we present the genome sequence of this strain, which is 5,550,230 bp with 4,722 genes and an average G+C content of 39.7%.


2020 ◽  
Vol 9 (47) ◽  
Author(s):  
Mitchell Henry Wright ◽  
Steven Robert Bentley ◽  
Anthony Carlson Greene

ABSTRACT Halomonas sp. strain ML-15 is an aerobic, haloalkaliphilic bacterium capable of degrading polycyclic aromatic hydrocarbons (PAHs). The draft genome sequence of the isolate contains 19 contigs encompassing 4.8 Mb and a G+C content of 65.38%. This sequence will provide essential information for future studies of PAH degradation, particularly under haloalkaliphilic conditions.


2013 ◽  
Vol 664 ◽  
pp. 72-76 ◽  
Author(s):  
Jie Yu

The degradation of polycyclic aromatic hydrocarbons in sludge in aerobic fermentation process is affected by many factors, and the pile body pH value is one of the important factors. Through the test of mixing lime pile body regulating pH value (7.7, 8.2, 9), the polycyclic aromatic hydrocarbon degradation effect was researched under the different pH conditions and the same of the other aerobic fermentation conditions. The results showed that, pH=7.7 test device (not mixing lime ) on PAHs degradation effect was best, worst was the pH=8.2 test device, the sludge degradation of the polycyclic aromatic hydrocarbons effect is negative effect.


2014 ◽  
Vol 665 ◽  
pp. 563-566
Author(s):  
Xin Xin Wang ◽  
Liang Wu ◽  
Ping Zhou ◽  
Chen Li ◽  
Li Bin Zhao ◽  
...  

Magnetic flocculation was reported to be efficient to harvest microalgae. However, little is known about the recovery of PAHs-degrading bacteria from the culture by magnetic flocculation.Advenella kashmirensisstrain w13003, a PAHs-degrading bacterium, was harvested by magnetic flocculation using magnetite and polyferric sulphate (PFS) as flocculants. The results showed the treatment with magnetite and PFS showed high concentration factor with flocculation efficiency near 100%, which suggest magnetic flocculation could result in a high concentration factor without significantly loss of PAHs-degrading bacteria. This is the first report about the recovery of PAHs-degrading bacteria by magnetic flocculation, which suggest its potential in bacterial harvesting.


2000 ◽  
Vol 66 (12) ◽  
pp. 5387-5392 ◽  
Author(s):  
Trevor Bugg ◽  
Julia M. Foght ◽  
Michael A Pickard ◽  
Murray R. Gray

ABSTRACT The mechanism of transport of polycyclic aromatic hydrocarbons (PAHs) by Pseudomonas fluorescens LP6a, a PAH-degrading bacterium, was studied by inhibiting membrane transport and measuring the resulting change in cellular uptake. Three cultures were used: wild-type LP6a which carried a plasmid for PAH degradation, a transposon mutant lacking the first enzyme in the pathway for PAH degradation, and a cured strain without the plasmid. Washed cells were mixed with aqueous solutions of radiolabelled PAH; then the cells were removed by centrifugation, and the concentrations of PAH in the supernatant and the cell pellet were measured. The change in the pellet and supernatant concentrations after inhibitors of membrane transport (azide, cyanide, or carbonyl cyanide m-chlorophenyl hydrazone) were added indicated the role of active transport. The data were consistent with the presence of two conflicting transport mechanisms: uptake by passive diffusion and an energy-driven efflux system to transport PAHs out of the cell. The efflux mechanism was chromosomally encoded. Under the test conditions used, neither uptake nor efflux of phenanthrene by P. fluorescens LP6a was saturated. The efflux mechanism showed selectivity since phenanthrene, anthracene, and fluoranthene were transported out of the cell but naphthalene was not.


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