The effect of leaf treatment (Solanum tuberosum L. cv Zhukovsky) with an early leaf extract (Amaranthus tricolor L. cv Early splendor) on the photosynthetic indicators of potato plants and the feeding of the colorado beetle larvae

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

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Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro)

JURNAL BIOS LOGOS ◽

10.35799/jbl.9.2.2019.24746 ◽

2019 ◽

Vol 9 (2) ◽

pp. 91

Author(s):

Ghea Dotulong ◽

Stella Umboh ◽

Johanis Pelealu

Keyword(s):

Solanum Tuberosum ◽

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Leaf Extract ◽

Solanum Tuberosum L ◽

In Vitro Toxicity ◽

Potato Plants ◽

Colony Diameter ◽

In Vitro Toxicity Test

Uji Toksisitas Beberapa Fungisida Nabati terhadap Penyakit Layu Fusarium (Fusarium oxysporum) pada Tanaman Kentang (Solanum tuberosum L.) secara In Vitro (Toxicity Test of several Biofungicides in controlling Fusarium wilt (Fusarium oxysporum) in Potato Plants (Solanum tuberosum L.) by In Vitro) Ghea Dotulong1*), Stella Umboh1), Johanis Pelealu1), 1) Program Studi Biologi, FMIPA Universitas Sam Ratulangi, Manado 95115*Email korespondensi: [email protected] Diterima 9 Juli 2019, diterima untuk dipublikasi 10 Agustus 2019 Abstrak Tanaman kentang (Solanum tuberosum L.) adalah salah satu tanaman hortikultura yang sering mengalami penurunan dari segi produksi dan produktivitasnya, akibat adanya serangan penyakit layu yang salah satunya disebabkan oleh Fusarium oxysporum. Tujuan penelitian ini adalah mengidentifikasi toksisitas beberapa fungisida nabati dalam mengendalikan penyakit Layu Fusarium (F. oxysporum) pada tanaman kentang (Solanum tuberosum L.) secara In Vitro. Metode Penelitian yang digunakan yaitu metode umpan beracun. Data dianalisis dengan Rancangan Acak Lengkap (RAL) dengan Analisis Varian (ANAVA) yang dilanjutkan dengan menggunakan metode BNT (Beda Nyata Terkecil). Hasil Penelitian, diperoleh nilai toksisitas fungisida nabati tertinggi yaitu pada ekstrak daun sirsak dengan nilai HR (69,44%), kategori berpengaruh, ditandai dengan diameter koloni 2,75 cm (100ppm) dan yang terendah toksisitasnya yaitu pada ekstrak daun jeruk purut dengan nilai HR (49,81%), kategori cukup berpengaruh ditandai dengan diameter koloni 3,75 cm (25ppm). Semakin tinggi konsentrasi yang diujikan maka semakin tinggi toksisitas dari fungisida nabati yang diberikan.Kata Kunci: fungisida nabati, Fusarium oxysporum, tanaman kentang, In Vitro Abstract Potato plants (Solanum tuberosum L.) is one of the horticulture plants which often decreases in terms of production and productivity, due to the attack of wilt, one of which is caused by Fusarium oxysporum. The purpose of this study was to determine the toxicity of several biofungicides in controlling Fusarium wilt (F. oxysporum) in potato plants (Solanum tuberosum L.) in Vitro. The research method used was the In Vitro method with the poison bait method. Data were analyzed by Completely Randomized Design with Variant Analysis (ANAVA), followed by the BNT method. The results showed that the highest biofungicide toxicity value was soursop leaf extract with HR values (69.44%), influential categories, characterized by colony diameter 2.75 cm (100ppm) and the lowest toxicity, namely in kaffir lime leaf extract with a value of HR (49.81%), quite influential category was characterized by colony diameter of 3.75 cm (25ppm). The higher the concentration tested, the higher the toxicity of the biofungicide given.Keywords: biofungicides, Fusarium oxysporum, Potato Plants, In Vitro.

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Uptake of Plutonium-238 into Solanum tuberosum L. (potato plants) in presence of complexing agent EDTA

Journal of Environmental Radioactivity ◽

10.1016/j.jenvrad.2017.08.007 ◽

2017 ◽

Vol 178-179 ◽

pp. 186-192

Author(s):

Frank Tawussi ◽

Dharmendra K. Gupta ◽

Elena L. Mühr-Ebert ◽

Stephanie Schneider ◽

Stefan Bister ◽

...

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L ◽

Complexing Agent ◽

Potato Plants

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Etude Des Effets Des Régulateurs De Croissance Sur La Les Stades De Prolifération Et De Développement De La Pomme De Terre (Solanum Tuberosum. L) In Vitro

European Scientific Journal ESJ ◽

10.19044/esj.2017.v13n24p145 ◽

2017 ◽

Vol 13 (24) ◽

pp. 145

Author(s):

Sadek Chahredine ◽

Nadia Ykhlef

Keyword(s):

Solanum Tuberosum ◽

Callus Induction ◽

Solanum Tuberosum L ◽

Naphthaleneacetic Acid ◽

Compact Structure ◽

Potato Plants ◽

Régulateurs De Croissance ◽

Role Of Light

The aim of this study is to determine the effects of different concentrations and combinations of the phytohormones, 1-naphthaleneacetic acid (NAA), and 6-benzylaminopurine (BAP): M1 (0.5 mg / l +1 mg / l), M2 (1 mg / l + 0.5 mg / l) , M3 (2 mg / l +2 mg / l), M4 (0.5 mg / l + l mg / l, NAA), M5 (1.0 mg / l + l mg / l , NAA), and M6 (2.0 mg / l + l mg / l, NAA). This study was carried out in dark condition on callus induction of potato plants (Solanum tuberosum L.) cultivars from potato tuber bud so as to demonstrate the role of light. The callus initiation begins after 7 days of incubation for all studied media. After two months of incubation, the better development of callus was noted in Spunta variety by using medium M1, M2, M3, and M6. The calluses took a compact structure of brown-white color for both varieties with a callus induction rate of 20- 40%. This was collected with kondor variety for M2 and (M3, M4, M5) media respectively and 10-30% for M4 (M1, M2, M3) for Spunta variety also. The highest fresh weight was recorded on M2 medium with 0.26g for Kondor variety and 0.93g for Spunta variety.

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ChemInform Abstract: Cyclic Sesquiterpenes in the Volatiles Secreted by Potato Leaves (Solanum tuberosum L.) and Colorado Beetle (Leptinotarsa decemplineata Say)

ChemInform ◽

10.1002/chin.199806222 ◽

2010 ◽

Vol 29 (6) ◽

pp. no-no

Author(s):

A. Z. KHALILOVA ◽

E. A. PARAMONOV ◽

U. A. BALTAEV ◽

V. N. ODINOKOV ◽

L. M. KHALILOV

Keyword(s):

Solanum Tuberosum ◽

Solanum Tuberosum L ◽

Colorado Beetle

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ANALISIS TINGKAT POPULASI JAMUR TANAH DI LAHAN PERTANAMAN KENTANG (Solanum tuberosum L.) BERDASARKAN METODE TOTAL PLATE COUNT (TPC)

JURNAL ILMIAH SAINS ◽

10.35799/jis.19.2.2019.23961 ◽

2019 ◽

Vol 19 (2) ◽

pp. 105

Author(s):

Windha Arantika ◽

Stella D. Umboh ◽

Johanis J. Pelealu

Keyword(s):

Solanum Tuberosum ◽

Soil Fungi ◽

Solanum Tuberosum L ◽

Soil Fungus ◽

Population Level ◽

Soil Samples ◽

Plate Count ◽

Potato Field ◽

Potato Plants ◽

Total Plate Count

Penelitian ini bertujuan untuk mengetahui tingkat populasi jamur tanah di lahan pertanaman kentang (S. tuberosum L.). Sampel tanah diambil di perkebunan tanaman kentang Desa Pinasungkulan Kecamatan Modoinding yang banyak menggunakan pestisida. Penelitian ini dilakukan dengan mengisolasi jamur pada tanah di pertanaman kentang di Desa Pinasungkulan, Kecamatan Modoinding, Kabupaten Minahasa Selatan dengan pengenceran bertingkat dan dilanjutkan dengan menghitung jumlah koloni dengan metode Total Plate Count (TPC). Dari hasil isolasi diperoleh 6 famili dengan 11 jenis jamur tanah yang memiliki koloni terbesar pada isolat KJ1 sebesar 71 koloni dan terendah pada isolat KJ8, KJ10, dan KJ 11 masing-masing sebesar 2 koloni.Kata kunci: Tanaman kentang, jamur tanah, tingkat populasi, Total Plate Count (TPC) ANALYSIS OF SOIL FUNGUS POPULATION LEVELS IN POTATO PLANTS (Solanum tuberosum L.) BASED ON TOTAL PLATE COUNT (TPC) METHODSABSTRACTThis study aimed to determine the level of soil fungus population on potato planting land (S. tuberosum L.). Soil samples were taken at a potato field plantation in Pinasungkulan Village, Modoinding Subdistrict, which used many pesticides. This research was carried out by isolating fungi on soil in potato plantations in Pinasungkulan Village, Modoinding District, South Minahasa Regency with multilevel dilution and continued by counting the number of colonies using the Total Plate Count (TPC) method. The result showed that the isolation obtained 6 families with 11 types of soil fungi that have the largest colony in KJ1 isolates of 71 colonies and the lowest in isolates KJ8, KJ10, and KJ 11 of 2 colonies respectively.Keywords: Potato plants, soil fungi, population level, Total Plate Count (TPC)

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The influence of jasmonic acid on the amount and the distribution of cysteine proteinase PLCP-2 in healthy and PVYNTN infected potato plants (Solanum tuberosum L.)

Plant Protection Science ◽

10.17221/10327-pps ◽

2002 ◽

Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽

pp. S95-S98

Author(s):

M. Pompe-Novak ◽

M. Tušek-Žnidarič ◽

B. Štrukelj ◽

M. Ravnikar

Keyword(s):

Electron Microscopy ◽

Solanum Tuberosum ◽

Jasmonic Acid ◽

Cell Walls ◽

Cysteine Proteinase ◽

Solanum Tuberosum L ◽

Cell Types ◽

Protein Bodies ◽

Potato Plants

The localization of cysteine proteinase PLCP-2 was investigated in potato plants (Solanum tuberosum L.) cultivar Désirée by electron microscopy. Healthy and PVY<sup>NTN</sup> infected potato plants were grown in vitro on media with or without a supplement of jasmonic acid. We had already shown that PLCP-2 is present in leaves, stems, tips of shoots and tips of roots of healthy and PVY<sup>NTN</sup> infected plants. It was detected in various cell types in protein bodies in vacuoles, in cytoplasm and in cell walls. There were significantly larger amounts of PLCP-2 in plants grown on medium with a supplement of jasmonic acid in both healthy and virus infected plants. More protein bodies in vacuoles were found in plants grown on medium with addition of jasmonic acid.

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