Increase Active Substances in Catharanthus Roseus L. G. Don with Water Tension and Foliar Application of Proline

Catharanthus roseus (L.) G. Don (Apocynaceae) is a medicinal plant that produces indole alkaloids used in cancer chemotherapy. Commercially important antineoplastic alkaloids, namely, vinblastine and vincristine, are mainly present in the leaves of C. roseus. Gamma-rays irradiated carrageenan (ICR) has been proven as plant growth promoting substance for a number of medicinal and agricultural plants. Considering the importance of ICR as a promoter of plant growth and alkaloids production in C. roseus, a pot experiment was carried out to explore the effect of ICR on the plant growth, physiological activities, and production of anticancer alkaloids in C. roseus at 120 and 150 days after planting (DAP). Foliar application of ICR (at 0, 20, 40, 60, 80, and 100 mg L−1) significantly improved the performance of C. roseus. 80 mg L−1 of ICR enhanced the leaf yield by 29.2 and 35.4% and the herbage yield by 32.5 and 37.4% at 120 and 150 DAP, respectively, over the control. The spray of ICR at 80 mg L−1 increased the yield of vinblastine by 64.3 and 65.0% and of vincristine by 75.5 and 77.0% at 120 and 150 DAP, respectively, as compared to the control.

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FORMATION OF THE ROOT SYSTEM IN PHYLLOXERA TOLERANT GRAPE VARIETIES WITH FOLIAR APPLICATION OF PHYSIOLOGICALLY ACTIVE SUBSTANCES

Fruit growing and viticulture of South Russia ◽

10.30679/2219-5335-2020-3-63-121-134 ◽

2020 ◽

Vol 3 (63) ◽

pp. 121-134

Author(s):

Ramidin Efendievich Kazakhmedov ◽

◽

Valeriy Semionovich Petrov ◽

Bahtiyar Mutalibovich Saidov ◽

Tamila Imiraslanovna Abdullayeva ◽

...

Keyword(s):

Root System ◽

Foliar Application ◽

Physiologically Active Substances ◽

Grape Varieties ◽

Active Substances

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EFFECT OF SALICYLIC AND HUMIC ACID ON VINCRISTINE AND N, P, K CONTENT IN MADAGASCAR PERIWINKLE LEAVES

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v47i2.600 ◽

2016 ◽

Vol 47 (2) ◽

Author(s):

Jaddo & Rabee

Keyword(s):

Salicylic Acid ◽

Humic Acid ◽

Catharanthus Roseus ◽

Foliar Application ◽

Block Design ◽

Experimental Unit ◽

Madagascar Periwinkle ◽

Randomized Block Design ◽

Two Factors ◽

Four Levels

An Experiment carried out in Al-Yarmook Area in Baghdad for the growing season 2014 to study the effects of foliar application of Salicylic acid and Humic acid on Vincristine and NPK content in Catharanthus roseus. A factorial experiment was applied including two factors according to The Complete Randomized Block Design, the first factor included four levels of salicylic acid 0, 25, 50, and 100 mg.L-1 which were represented with S0, S1, S2, and S3 sequentially, the second factor included three levels of Humic acid 0, 0.75, and 1.5 ml.L-1 and were represented with O0, O1, and O2 sequentially to make up 12 factors in total that were distributed randomly upon three replicates and each experimental unit included 8 plants. The results were analyzed using Genestat and the differences were tested according to (LSD) with a level of probability 5% and the results were as follows: Vincristine levels increased in the leaves of Catharanthus roseus as a result of foliar application of Salicylic acid and Humic as well NPK levels in leaves. Vinblastine was not detected in all treatments. The interaction effect between 100 mg.L-1 Salicylic acid and 1.5 ml.L-1Humic acid had an exceeding effect for all the studied parameters. It may be concluded that Salicylic and Humic acid may be used to increase Vincristine alkaloid content in Periwinkle plants at the levels 100 ml.L-1 and 1.5 mg.L-1 subsequently.

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Changing in the production of anticancer drugs (vinblastine and vincristine) in Catharanthus roseus (L.) G. Don by potassium and ascorbic acid treatments

Plant Soil and Environment ◽

10.17221/121/2021-pse ◽

2022 ◽

Author(s):

Neda Sahi ◽

Akbar Mostajeran ◽

Mustafa Ghanadian

Keyword(s):

Gene Expression ◽

Ascorbic Acid ◽

Anticancer Drugs ◽

Catharanthus Roseus ◽

Peroxidase Activity ◽

Foliar Application ◽

Control Plant ◽

Microtubule Assembly ◽

Positive Interaction ◽

Genes Expression

Catharanthus roseus seedling was treated with different concentrations (1.5, 3.16, 15, and 30 mmol) and forms (K2SO4 and KNO3) of potassium (K+) via Hoagland’s nutrient solution. Ascorbic acid (AsA) was sprayed twice (plant days 68 and 78) with different concentrations (750 and 1 500 mg/L) on the leaves. Vinblastine, vincristine, tryptophan contents, D4H and DAT genes expression, peroxidase activity, and H2O2 content of leaves were measured. Potassium in KNO3 form increased vinblastine (60%) and vincristine (50%), compared to 30% and 20% using K2SO4. Vinblastine and vincristine inhibit microtubule assembly and ultimately metaphase-arrested caused by the polymerisation. The genes expression was higher 3 times in KNO3 and 2.5 times in K2SO4 in excess of K+. Foliar application of 750 mg/L AsA led to an increase in vinblastine (20%) and vincristine (16%). Both concentrations of AsA had the same additional effect on the expression of D4H and DAT about 30% and 60%, respectively, compared to the control plant. Tryptophan decreased 2.5 times in excess of K+ and 35% due to the exterior of AsA. H2O2 decreased while peroxidase activity increased along with AsA treatment. A positive interaction existed between the K+ and AsA on the amount of vinblastine, vincristine, tryptophan, and gene expression.

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