scholarly journals Changing in the production of anticancer drugs (vinblastine and vincristine) in Catharanthus roseus (L.) G. Don by potassium and ascorbic acid treatments  

2022 ◽  
Author(s):  
Neda Sahi ◽  
Akbar Mostajeran ◽  
Mustafa Ghanadian
Keyword(s):  
Gene Expression ◽  
Ascorbic Acid ◽  
Anticancer Drugs ◽  
Catharanthus Roseus ◽  
Peroxidase Activity ◽  
Foliar Application ◽  
Control Plant ◽  
Microtubule Assembly ◽  
Positive Interaction ◽  
Genes Expression

Catharanthus roseus seedling was treated with different concentrations (1.5, 3.16, 15, and 30 mmol) and forms (K<sub>2</sub>SO<sub>4</sub> and KNO<sub>3</sub>) of potassium (K<sup>+</sup>) via Hoagland’s nutrient solution. Ascorbic acid (AsA) was sprayed twice (plant days 68 and 78) with different concentrations (750 and 1 500 mg/L) on the leaves. Vinblastine, vincristine, tryptophan contents, D4H and DAT genes expression, peroxidase activity, and H<sub>2</sub>O<sub>2</sub> content of leaves were measured. Potassium in KNO<sub>3</sub> form increased vinblastine (60%) and vincristine (50%), compared to 30% and 20% using K<sub>2</sub>SO<sub>4</sub>. Vinblastine and vincristine inhibit microtubule assembly and ultimately metaphase-arrested caused by the polymerisation. The genes expression was higher 3 times in KNO<sub>3</sub> and 2.5 times in K<sub>2</sub>SO<sub>4</sub> in excess of K<sup>+</sup>. Foliar application of 750 mg/L AsA led to an increase in vinblastine (20%) and vincristine (16%). Both concentrations of AsA had the same additional effect on the expression of D4H and DAT about 30% and 60%, respectively, compared to the control plant. Tryptophan decreased 2.5 times in excess of K<sup>+</sup> and 35% due to the exterior of AsA. H<sub>2</sub>O<sub>2</sub> decreased while peroxidase activity increased along with AsA treatment. A positive interaction existed between the K<sup>+</sup> and AsA on the amount of vinblastine, vincristine, tryptophan, and gene expression.  

Low Light Affacting Alkaloids Accumulation and Related Biosynthetic Pathway Genes Expression in Leaves of Catharanthus roseus Seedlings

2014 ◽  
Vol 522-524 ◽  
pp. 311-315
Author(s):  
Ying Liu ◽  
De Wen Li ◽  
Zhong Hua Zhang ◽  
Yuan Gang Zu
Keyword(s):  
Gene Expression ◽  
Catharanthus Roseus ◽  
High Performance ◽  
Light Condition ◽  
Content Increase ◽  
Biosynthesis Pathway ◽  
Expression Change ◽  
Low Light ◽  
Plastic Films ◽  
Genes Expression

The alkaloids contents were investigated by High performance liquid chromatography (HPLC) in Catharanthus roseus aseptic seedlings leaves, the expressions of terpenoid indole alkaloids (TIAs) biosynthesis pathway genes were detected by semi-quantitative RT-PCR. The plastic films treatment induces a low light condition. HPLC analysis shows, the contents of vindoline (VIN) and catharanthine (CAT) are slight increase first and significant decrease (p<0.05) under plastic films, the vinblastine (VBL) content increase and reach the maximum (0.028 mg g-1±0.0051) on the 15th day of treatment. TIA biosynthesis pathway genes expression change under low light. The correlation analysis indicates, VIN accumulation is significantly correlated with the gene expression of desacetoxyvindoline-4-hydroxylase (D4h) and deacetylvindoline-4-O-acetyl transferase (Dat), and CAT accumulation is significantly correlated with strictosidine synthase (Str) gene expression (p<0.05). The low light treatment with plastic films accelerates the accumulation of VBL in C. roseus seedlings leaves.

Respon Morfologis dan Ekspresi Gen Aquaporin pada Padi IR 64 yang Mengalami Cekaman Kekeringan pada Fase Reproduktif (Morphological Responses and Aquaporin Gene Expression in Rice IR64 under Drought Stress at the Reproductive Stage)

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
Made Pharmawati ◽  
Ni Nyoman Wirasiti ◽  
Luh Putu Wrasiati
Keyword(s):  
Gene Expression ◽  
Drought Stress ◽  
Real Time ◽  
Real Time Pcr ◽  
Control Plant ◽  
Reproductive Stage ◽  
Limiting Factors ◽  
Rice Grain ◽  
Aquaporin Gene ◽  
Peg Treatment

Abstrak Cekaman kekeringan merupakan faktor pembatas penting bagi pertumbuhan dan produktivitas tanaman termasuk padi.      Penelitian ini bertujuan menganalisis respon padi IR64 terhadap cekaman kekeringan dengan pemberian polietilen glikol (PEG) pada fase reproduktif.  Penelitian juga bertujuan menganalisis ekspresi gen aquaporin akibat cekaman kekeringan.  Bibit padi ditanam dalam pot dan perlakuan PEG dengan konsentrasi 108g/L (-0.25MPa) dan 178g/L (-0.52 MPa) diberikan saat munculnya panikula. Perlakuan diberikan selama 2 minggu, kemudian tanaman disiram kembali.  Ekspresi gen diamati pada akhir perlakuan dengan semi kuantitatif real time PCR.  Ekstraksi RNA menggunakan RNeasy plant mini kit, sedangkan sintesis cDNA menggunakan Transcriptor First Strand cDNA Kit.  Hasil penelitian menunjukkan bahwa jumlah malai dan berat total malai berkurang akibat cekaman kekeringan.  Persentase gabah kosong mencapai 84,6% pada perlakuan PEG-0,52 MPa, sedangkan pada perlakuan PEG -0,25 MPa persentase gabah kosong sebesar 67,8%.  Pada kontrol persentase gabah kosong adalah 10,3%.  Ekspresi gen OsPIP2;7 sedikit menurun pada perlakuan PEG -0,52 MPa.Kata kunci: ekspresi gen, IR64, kekeringan, padi, PEG  Abstract Drought stress is one of the limiting factors of plant growth and productivity including rice.  The aim of this study was to analyze responses of IR64 rice to polyethylene glycol (PEG)-induced-drought stress at the reproductive stage.  This study also aimed to analyze the expression of aquaporin under drought stress.  Rice seedlings were grown in pot system and PEG treatment at concentration of -0.25MPa (108g/L) and -0.52 MPa (178g/L) were given when the panicles arose.  Treatments were conducted for 2 weeks, after that the plants were rewatered.  Gene expression was evaluated at the end of PEG treatment using semi quantitative real time PCR. RNA was extracted using RNeasy plant mini kit, while cDNA synthesis was done using Transcriptor First Strand cDNA Kit.  The results showed that the number and weight of rice ear were less in plant treated with PEG than in control.  The percentage of empty rice grain reached 84.6% at PEG -0.52 MPa, while at PEG -0.25 MPa the percentage of empty grain was 67.8%.  In control plant, the percentage of empty grain was 10.3%.  Drought stress did not alter the expression of OsPIP2;7.  Keywords: drought, gene expression, IR64, PEG, rice

An ESR study of the peroxidase reaction catalyzed by human methaemoglobin and methaemoglobin-haptoglobin complex

1991 ◽  
Vol 56 (4) ◽  
pp. 923-932
Author(s):  
Jana Stejskalová ◽  
Pavel Stopka ◽  
Zdeněk Pavlíček
Keyword(s):  
Hydrogen Peroxide ◽  
Ascorbic Acid ◽  
Amino Acid ◽  
Peroxidase Activity ◽  
Amino Acid Analysis ◽  
Superoxide Radical ◽  
Esr Spectra ◽  
The Other ◽  
Delocalized Electron

The ESR spectra of peroxidase systems of methaemoglobin-ascorbic acid-hydrogen peroxide and methaemoglobin-haptoglobin complex-ascorbic acid-hydrogen peroxide have been measured in the acetate buffer of pH 4.5. For the system with methaemoglobin an asymmetrical signal with g ~ 2 has been observed which is interpreted as the perpendicular region of anisotropic spectrum of superoxide radical. On the other hand, for the system with methaemoglobin-haptoglobin complex the observed signal with g ~ 2 is symmetrical and is interpreted as a signal of delocalized electron. After realization of three repeatedly induced peroxidase processes the ESR signal of the perpendicular part of anisotropic spectrum of superoxide radical is distinctly diminished, whereas the signal of delocalized electron remains practically unchanged. An amino acid analysis of methaemoglobin along with results of the ESR measurements make it possible to derive a hypothesis about the role of haptoglobin in increasing of the peroxidase activity of methaemoglobin.

scholarly journals Effects of Irrigation Deficit and Application of Some Growth Regulators on Defense Mechanisms of Canola

2015 ◽  
Vol 43 (1) ◽  
pp. 124-130 ◽  
Author(s):  
Seyed Ahmad KALANTAR AHMADI ◽  
Ali EBADI ◽  
Jahanfar DANESHIAN ◽  
Soodabeh JAHANBAKHSH ◽  
Seyed Ataollah SIADAT ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Deficit Irrigation ◽  
Defense Mechanisms ◽  
Agricultural Research ◽  
Foliar Application ◽  
Maximum Activity ◽  
Flowering Stage ◽  
Relative Water ◽  
Irrigation Deficit ◽  
Main Factor

A split-plot experiment arranged in a randomized complete blocks design with three replications was carried out in Safiabad Agricultural Research Center of Dezful in order to investigate the effects of foliar applications of ascorbic acid (AsA), salicylic acid (SA) and methanol (Me), under deficit irrigation conditions, in canola; there were 3 levels of irrigation as the main factor (irrigation after 70 mm evaporation from the pan as control, cessation of irrigation at the flowering stage and cessation of irrigation at the appearance of siliques) and 10 levels of foliar applications as sub-factor (100, 200 and 300 mg.l-1AsA; 100, 200 and 300 µM SA; 10, 20 and 30% (w/v) methanol; and foliar application of distilled water as control). Foliar applications were made during both budding and initiation of flowering stages. Results indicated that antioxidant enzymes showed different responses to deficit irrigation and foliar application treatments. Maximum catalase (CAT) and polyphenol oxidase (PPO) activities were observed under cessation of irrigation at flowering stage and foliar application of 300 µM SA, while ascorbate peroxidase (APX) reached its maximum activity under the same irrigation conditions and foliar application of 300 mg.l-1AsA. SA had more influence to increase in CAT and PPO activity under cessation of irrigation at flowering stage. The relative water content (RWC) was also decreased due to the drought stress caused by the cessation of irrigation. Foliar application of SA (100 μM) and Me (10% w/v) had more influence to maintain RWC compared to ascorbic acid under irrigation cessation at flowering stage.

Anti-Oxidant Activities, Chemical Attributes and Fruit Yield of Peach Cultivars as Influenced by Foliar Application of Ascorbic Acid

2017 ◽  
Vol 69 (3) ◽  
pp. 113-121 ◽  
Author(s):  
Muhammad Sajid ◽  
Muhammad Ali Khan ◽  
Wasim Bilal ◽  
Abdur Rab ◽  
Zafar Iqbal ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Foliar Application ◽  
Fruit Yield ◽  
Anti Oxidant ◽  
Chemical Attributes

scholarly journals Radiation Processed Carrageenan Improves Plant Growth, Physiological Activities, and Alkaloids Production in Catharanthus roseus L.

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
M. Naeem ◽  
Mohd. Idrees ◽  
Tariq Aftab ◽  
M. Masidur Alam ◽  
M. Masroor A. Khan ◽  
...  
Keyword(s):  
Plant Growth ◽  
Catharanthus Roseus ◽  
Gamma Rays ◽  
Foliar Application ◽  
Indole Alkaloids ◽  
Leaf Yield ◽  
Plant Growth Promoting ◽  
Commercially Important ◽  
Agricultural Plants ◽  
Growth Promoting

Catharanthus roseus (L.) G. Don (Apocynaceae) is a medicinal plant that produces indole alkaloids used in cancer chemotherapy. Commercially important antineoplastic alkaloids, namely, vinblastine and vincristine, are mainly present in the leaves of C. roseus. Gamma-rays irradiated carrageenan (ICR) has been proven as plant growth promoting substance for a number of medicinal and agricultural plants. Considering the importance of ICR as a promoter of plant growth and alkaloids production in C. roseus, a pot experiment was carried out to explore the effect of ICR on the plant growth, physiological activities, and production of anticancer alkaloids in C. roseus at 120 and 150 days after planting (DAP). Foliar application of ICR (at 0, 20, 40, 60, 80, and 100 mg L−1) significantly improved the performance of C. roseus. 80 mg L−1 of ICR enhanced the leaf yield by 29.2 and 35.4% and the herbage yield by 32.5 and 37.4% at 120 and 150 DAP, respectively, over the control. The spray of ICR at 80 mg L−1 increased the yield of vinblastine by 64.3 and 65.0% and of vincristine by 75.5 and 77.0% at 120 and 150 DAP, respectively, as compared to the control.

scholarly journals Diabetes Induced Renal Complications by Leukocyte Activation of Nuclear Factor κ-B and its Regulated Genes Expression

2020 ◽  
Author(s):  
Noura Darwish ◽  
Yousif Elnahas ◽  
Fatmah AlQahtany
Keyword(s):  
Gene Expression ◽  
Large Scale ◽  
Structural Changes ◽  
Kidney Injury ◽  
Leukocyte Activation ◽  
Disease Biomarkers ◽  
Genes Expression ◽  
Nuclear Factor Κ B ◽  
Adhesion Process

Type 2 diabetes mellitus (T2D) is a metabolic disorder characterized by inappropriate insulin function. Despite wide progress in genome studies, defects in gene expression for diabetes prognosis still incompletely identified. Prolonged hyperglycemia activates NF-&kappa;B, which is a main player in vascular dysfunctions of diabetes. Activated NF-&kappa;B, triggers expression of various genes that promote inflammation and cell adhesion process. Alteration of pro-inflammatory and profibrotic gene expression contribute to the irreversible functional and structural changes in the kidney resulting in diabetic nephropathy (DN). To identify the effect of some important NF-&kappa;B related genes on mediation of DN progression, we divided our candidate genes on the basis of their function exerted in bloodstream into three categories (Proinflammatory; NF-&kappa;B, IL-1B, IL-6, TNF-&alpha; and VEGF); (Profibrotic; FN, ICAM-1, VCAM-1) and (Proliferative; MAPK-1 and EGF). We analyzed their expression profile in leukocytes of patients and explored their correlation to diabetic kidney injury features. Our data revealed the overexpression of both proinflammatory and profibrotic genes in DN group when compared to T2D group and were associated positively with each other in DN group indicating their possible role in DN progression. In DN patients, increased expression of proinflammatory genes correlated positively with glycemic control and inflammatory markers indicating their role in DN progression. Our data revealed that the persistent activation NF-&kappa;B and its related genes observed in hyperglycemia might contribute to DN progression and might be a good diagnostic and therapeutic target for DN progression. Large-scale studies are needed to evaluate the potential of these molecules to serve as disease biomarkers.

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