scholarly journals Segregation ratio and heritability of agronomic character in yardlong bean F2 population

2021 ◽  
Vol 803 (1) ◽  
pp. 012027
Author(s):  
Florentina Kusmiyati ◽  
Syaiful Anwar ◽  
Urip Jamiati ◽  
Bagus Herwibawa
Keyword(s):  
Segregation Ratio ◽  
F2 Population ◽  
Agronomic Character ◽  
Yardlong Bean

scholarly journals Agronomic character evaluation of F3 Yardlong bean progenies

2021 ◽  
Author(s):  
F. Kusmiyati ◽  
S. Anwar ◽  
B. Herwibawa
Keyword(s):  
Agronomic Character ◽  
Yardlong Bean ◽  
Character Evaluation

scholarly journals Genome sequencing identified novel mechanisms underlying virescent mutation in upland cotton Gossypiuma hirsutum

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Jin Gao ◽  
Yang Shi ◽  
Wei Wang ◽  
Yong-Hui Wang ◽  
Hua Yang ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Molecular Mechanisms ◽  
Bulked Segregant Analysis ◽  
Protoporphyrin Ix ◽  
Segregation Ratio ◽  
Chlorophyll Synthesis ◽  
Start Codon ◽  
Real Time Quantitative Pcr ◽  
F2 Population ◽  
Virescent Mutant

Abstract Background Virescent mutation broadly exists in plants and is an ideal experimental material to investigate regulatory mechanisms underlying chlorophyll synthesis, photosynthesis and plant growth. Up to date, the molecular mechanisms in two virescent mutations have been clarified in cottons (Gossypiuma hirsutum). A virescent mutation has been found in the cotton strain Sumian 22, and the underlying molecular mechanisms have been studied. Methods The virescent mutant and wild type (WT) of Sumian 22 were cross-bred, and the F1 population were self-pollinated to calculate the segregation ratio. Green and yellow leaves from F2 populations were subjected to genome sequencing and bulked-segregant analysis was performed to screen mutations. Real-time quantitative PCR (RT-qPCR) were performed to identify genes in relations to chlorophyll synthesis. Intermediate products for chlorophyll synthesis were determined to validate the RT-qPCR results. Results The segregation ratio of green and virescent plants in F2 population complied with 3:1. Compared with WT, a 0.34 Mb highly mutated interval was identified on the chromosome D10 in mutant, which contained 31 genes. Among them, only ABCI1 displayed significantly lower levels in mutant than in WT. Meanwhile, the contents of Mg-protoporphyrin IX, protochlorophyllide, chlorophyll a and b were all significantly lower in mutant than in WT, which were consistent with the inhibited levels of ABCI1. In addition, a mutation from A to T at the -317 bp position from the start codon of ABCI1 was observed in the genome sequence of mutant. Conclusions Inhibited transcription of ABCI1 might be the mechanism causing virescent mutation in Sumian 22 cotton, which reduced the transportation of protoporphyrin IX to plastid, and then inhibited Mg-protoporphyrin IX, Protochlorophyllide and finally chlorophyll synthesis. These results provided novel insights into the molecular mechanisms underlying virescent mutation in cotton.

scholarly journals Genetic resistance of common bean cultivar Beija Flor to Colletotrichum lindemuthianum

2020 ◽  
Vol 43 ◽  
pp. e44910
Author(s):  
João Ricardo Silva Marcon ◽  
Maria Celeste Gonçalves Vidigal ◽  
Jean Fausto Carvalho Paulino ◽  
Pedro Soares Vidigal Filho ◽  
Marcela Coêlho
Keyword(s):  
Common Bean ◽  
Genetic Resistance ◽  
Segregation Ratio ◽  
Colletotrichum Lindemuthianum ◽  
Anthracnose Resistance ◽  
Bean Cultivar ◽  
F2 Population ◽  
Allelism Tests ◽  
Common Bean Cultivar ◽  
The Common

Anthracnose, which is caused by the fungus Colletotrichum lindemuthianum, is one of the most widespread and important diseases of the common bean (Phaseolus vulgaris L.) in the world. The objective of the present study was to characterize the genetic resistance of the Beija Flor cultivar by inheritance and to conduct allelism tests. The inheritance test was conducted in the F2 population derived from the Beija Flor (resistant) x TU (susceptible) cross inoculated with race 2047 of C. lindemuthianum. Furthermore, allelism tests exhibited a fitted segregation ratio of 15R:1S, thereby indicating the independence of the Beija Flor gene from the following previously characterized genes: Co-1, Co-2, Co-4, Co-42, Co-6, Co-12, Co-14, Co-15, and Co-Pe. Based on the aforementioned results, we are proposing the symbol Co-Bf to designate the new anthracnose resistance gene in the Brazilian Andean common bean cultivar Beija Flor. This cultivar is an important source of resistance to C. lindemuthianum that should provide a valuable contribution to the common bean breeding program for anthracnose resistance.

scholarly journals THE SEGREGATION PATTERN OF INSECT RESISTANCE GENES IN THE PROGENIES AND CROSSES OF TRANSGENIC ROJOLELE RICE

2016 ◽  
Vol 9 (2) ◽  
pp. 35 ◽  
Author(s):  
Satoto Satoto ◽  
Yuli Sulistyowati ◽  
Alex Hartana ◽  
Inez H. Slamet -Loedin
Keyword(s):  
Insect Resistance ◽  
Rice Variety ◽  
Stem Borer ◽  
Segregation Ratio ◽  
Mendelian Inheritance ◽  
Segregation Pattern ◽  
Chi Square ◽  
F2 Population ◽  
Chi Square Test ◽  
Brown Plant Hopper

Successful application of genetic transformation technique, especially in developing rice variety resistant to brown plant hopper and stem borer, will depend on transgene being expressed and the gene inherited in a stable and predictable manner. This study aimed to analyse transgene segregation pattern of the progenies and the crosses of transgenic rice cv. Rojolele harboring cry1Ab and gna genes. The third generation (T2) of five<br />transgenic Rojolele events containing gna and/or cry1Ab were evaluated for two generations to identify the homozygous lines and to study their inheritance. The homozygous lines were selected based on the result of PCR technique. The segregation patterns of gna and cry1Ab were studied in eight F2 populations derived from Rojolele x transgenic Rojolele homozygous for cry1Ab and or gna and their reciprocal crosses. Data  resulted from PCR of F2 population were analysed using a Chi Square test. The study obtained six homozygous lines for gna, namely A22- 1-32, A22-1-37, C72-1-9, F11-1-48, K21-1-39, K21-1-48, and two homozygous lines for cry1Ab, namely K21-1-39 and K21- 1-48. Both cry1Ab and gna transgenes had been inherited through selfing and crossing with their wild type as indicated from the F1 containing gna and cry1Ab as many as 48.4% and 47.4%, respectively. In six of the eight crosses, gna was inherited in a 3:1 ratio consistent with Mendelian inheritance of a single dominant locus, while in the remaining two crosses, gna was segregated in a 1:1 ratio. The presence of cry1Ab in F2 populations also showed a 3:1 segregation ratio in all crosses. In the F2 population derived from F1 plant containing cry1Ab and gna, both transgenes segregated in a 9:3:3:1 dihybrid segregation ratio. This study will add to the diversity of genetic sources for insect resistance and allow further use of these transgenic lines for pyramiding resistance to brown plant hopper and stem borer or  separately in rice breeding programs whenever the efficacy tests and biosafety requirements have been completed.

scholarly journals Molecular Markers for Septoria Leaf Spot (Septoria lycopersicii Speg.) Resistance in Tomato (Solanum lycopersicum L.)

2015 ◽  
Vol 3 (1) ◽  
pp. 40-47
Author(s):  
Bal K Joshi ◽  
Frank J Louws ◽  
G Craig Yenco ◽  
Byron R Sosinski ◽  
Consuelo Arellano ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Solanum Lycopersicum ◽  
Leaf Spot ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Dominant Gene ◽  
Segregation Ratio ◽  
Solanum Lycopersicum L ◽  
F2 Population ◽  
Septoria Leaf Spot

Marker assisted selection (MAS) has not been initiated in tomato (Solanum lycopersicum L.) for septoria leaf spot (SLS) resistance caused by Septoria lycopersici Speg due to lack of molecular markers. We studied the inheritance of SLS resistance and identified molecular markers linked to SLS resistance using bulked segregant analysis (BSA) in a segregating F2 population. Tomato inbred lines, NC 85L-1W (2007), susceptible to SLS and NC 839-2(2007)-1, resistant to SLS were used to develop the segregating population. A total of 250 F2 plants, and 10 plants each of P1, P2 and F1 were grown at the Mountain Horticultural Crops Research and Extension Center (MHCREC), Mills River NC in the summer of 2009. Disease severity was scored using a scale of 0 to 5, where 0 = no disease and 5 = complete development of disease. DNA was extracted from 2-3 week old plants and parental lines were screened with a total of 197 random amplified polymorphic DNA (RAPD) primers, of which 34 were polymorphic. Two DNA bulks, called resistant bulk (RB) and susceptible bulk (SB) were prepared from the F2 individuals. The RB and SB consisted of 8 individuals each with disease scores of 0, and 4.0 or 4.5, respectively. The segregation ratio of resistant and susceptible plants in F2 generation fit the expected Mendelian ratio of 3:1 for a single dominant gene. Five RAPD markers were linked to the SLS disease reaction, of which two were linked to susceptibility and three to the resistance. Subject to verification in independent populations, these markers may be useful for MAS of SLS resistance in tomato.Nepal Journal of Biotechnology. Dec. 2015 Vol. 3, No. 1: 40-47

scholarly journals THE SEGREGATION PATTERN OF INSECT RESISTANCE GENES IN THE PROGENIES AND CROSSES OF TRANSGENIC ROJOLELE RICE

2016 ◽  
Vol 9 (2) ◽  
pp. 35
Author(s):  
Satoto Satoto ◽  
Yuli Sulistyowati ◽  
Alex Hartana ◽  
Inez H. Slamet -Loedin
Keyword(s):  
Insect Resistance ◽  
Rice Variety ◽  
Stem Borer ◽  
Segregation Ratio ◽  
Mendelian Inheritance ◽  
Segregation Pattern ◽  
Chi Square ◽  
F2 Population ◽  
Chi Square Test ◽  
Brown Plant Hopper

Successful application of genetic transformation technique, especially in developing rice variety resistant to brown plant hopper and stem borer, will depend on transgene being expressed and the gene inherited in a stable and predictable manner. This study aimed to analyse transgene segregation pattern of the progenies and the crosses of transgenic rice cv. Rojolele harboring cry1Ab and gna genes. The third generation (T2) of five<br />transgenic Rojolele events containing gna and/or cry1Ab were evaluated for two generations to identify the homozygous lines and to study their inheritance. The homozygous lines were selected based on the result of PCR technique. The segregation patterns of gna and cry1Ab were studied in eight F2 populations derived from Rojolele x transgenic Rojolele homozygous for cry1Ab and or gna and their reciprocal crosses. Data  resulted from PCR of F2 population were analysed using a Chi Square test. The study obtained six homozygous lines for gna, namely A22- 1-32, A22-1-37, C72-1-9, F11-1-48, K21-1-39, K21-1-48, and two homozygous lines for cry1Ab, namely K21-1-39 and K21- 1-48. Both cry1Ab and gna transgenes had been inherited through selfing and crossing with their wild type as indicated from the F1 containing gna and cry1Ab as many as 48.4% and 47.4%, respectively. In six of the eight crosses, gna was inherited in a 3:1 ratio consistent with Mendelian inheritance of a single dominant locus, while in the remaining two crosses, gna was segregated in a 1:1 ratio. The presence of cry1Ab in F2 populations also showed a 3:1 segregation ratio in all crosses. In the F2 population derived from F1 plant containing cry1Ab and gna, both transgenes segregated in a 9:3:3:1 dihybrid segregation ratio. This study will add to the diversity of genetic sources for insect resistance and allow further use of these transgenic lines for pyramiding resistance to brown plant hopper and stem borer or  separately in rice breeding programs whenever the efficacy tests and biosafety requirements have been completed.

scholarly journals Genetic Diversity Analysis and F2 Population Development for Breeding of Long Juvenile Trait in Soybean

2018 ◽  
Vol 14 (1) ◽  
pp. 11 ◽  
Author(s):  
I Made Tasma ◽  
N. P. Mega Gena Yani ◽  
Rosliana Purwaningdyah ◽  
Dani Satyawan ◽  
Kristianto Nugroho ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ssr Markers ◽  
Segregation Ratio ◽  
Genetic Distances ◽  
Day Length ◽  
Diversity Analysis ◽  
Genetic Diversity Analysis ◽  
F2 Population ◽  
Soybean Genotypes ◽  
The Usa

<p>Genetic diversity analysis using molecular markers is an important step for selecting appropriate parents in a soybean breeding program. The aims of this study were to (1) analyze genetic diversity of 29 soybean genotypes assessed with 27 SSR markers for selecting appropriate parents and (2) develop F2 populations to be used for breeding long juvenile (LJ) trait in soybean to<br />be cultivated in short photoperiod condition. The soybean genotypes used consisted of 11 Indonesian soybean genotypes and 18 genotypes introduced from the USA. F2 populations were developed by crossing Grobogan with three introduced genotypes carrying LJ character. The PIC values of the 27 SSR markers ranged from 0.87 to 0.96. Cluster analysis resulted in three main<br />clusters at coefficient similarity of 0.76. The five LJ introduced accessions and the nine Indonesian genotypes showed high genetic distances and are useful as parent pairs for developing breeding populations. The F1 progeny phenotypic<br />performances of the cross far exceeded the performaces of both parents. Three F2 populations were developed by crossing the distantly related soybean genotypes. The F2 populations were verified by using SSR markers and it was found that they segregated in a 1:2:1 ratio confirming the segregation ratio of codominant SSR markers. The F2 populations should be useful for breeding LJ characters to improve soybean productivity in low latitude tropical countries such as Indonesia, which has day length of approximately 12 h all year round.</p>

Genetic Mapping of a Soybean Glabrous Gene Using Specific-Locus Amplified Fragment Sequencing Method

2019 ◽  
Author(s):  
Xiaomin Yu ◽  
Hangxia Jin ◽  
Qinghua Yang ◽  
Xujun Fu ◽  
Fengjie Yuan
Keyword(s):  
Candidate Genes ◽  
High Throughput Sequencing ◽  
Dominant Gene ◽  
Segregation Ratio ◽  
Chromosome 9 ◽  
Sequencing Technology ◽  
Selection Procedures ◽  
F2 Population ◽  
Sequencing Method ◽  
Specific Locus

Trichome is a natural trait related with plant defense and seed yield, however, the genetic control of this trait has not been extensively studied in soybean. In this study, a cross was made between a soybean landrace “Aijiaomaodou” with normal pubescence and a glabrous soybean line “39002”. The segregation ratio of the F2 population was fit to the expected value and the glabrous phenotype was controlled by a single dominant gene. Association analysis was conducted to identify candidate genes for the glabrous trait using a high-throughput sequencing technology, specific-locus amplified fragment sequencing. A total of 6,961 polymorphic specific-locus amplified fragments were analyzed and a region of 2.76 Mb at nucleotides 47,315,610-50,072,564 on chromosome 9 containing 551 candidate genes was identified. Four specific genes (Glyma09G280500, Glyma09G282600, Glyma09G259400 and Glyma09G283400) in the region appeared to be most important candidate genes that are highly related to the glabrous trait. Further studies of these genes may lead to isolation and cloning of the glabrous gene and facilitate the selection procedures in soybean breeding.

Laboratory Exercise on the Segregation of Flower Color and Related Genes Using Salpiglossis sinuata

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 555b-555
Author(s):  
Chiwon W. Lee
Keyword(s):  
Phenotypic Expression ◽  
Pollen Grains ◽  
Flower Color ◽  
Segregation Ratio ◽  
Crop Breeding ◽  
Flower Opening ◽  
Pigmentation Pattern ◽  
Laboratory Exercise ◽  
Demonstration Plant ◽  
Corolla Color

Velvet flower (Salpiglossis sinuata, Solanaceae) can be used as an excellent demonstration plant for horticultural crop breeding classes. Salpiglossis produces large trumpet-like flowers exhibiting an assortment of corolla color and pigmentation pattern. The pistil is large (3 to 4 cm long) with a sticky stigmatal tip and anthers can be easily emasculated prior to anthesis. The large pollen grains are shed in tetrads, which can be separated and individually placed on the stigma. It takes 8 to 9 weeks from seeding to blooming, with a prolific flowering cycle repeated in flushes. Numerous seeds (about 750/capsule) are obtained in 3 weeks after self- or cross-pollination. The influences of three genes that control flower color and pigmentation pattern can be conveniently demonstrated with their dominant and recessive alleles. The R gene controls flower color with red (RR or Rr) being dominant over yellow (rr) flower color. The D gene controls the density of pigmentation with solid (DD or Dd) color being dominant over dilute (dd) color. Corolla color striping is controlled by the St gene with striped (stst) being recessive to non-striped (StSt or Stst) pattern. For example, by using diploid lines of genotypes RRDD (red, solid), RRdd (red, dilute), or rrdd (yellow, dilute) and their crosses, students can easily learn a dominant phenotypic expression in the F1 hybrid and the digenic 9:3:3:1 segregation ratio in the F2 progeny. Another gene (C) that controls flower opening can also be used to show its influence on cleistogamous (closed, self-pollinated, CC or Cc) versus normal chasmogamous (open-pollinated, cc) corolla development. In addition, the induction and use of polyploid (4X, 3X) plants in plant breeding can be effectively demonstrated using this species.

Sign in / Sign up

Export Citation Format

Share Document