Effects of Di-2-ethylhexyl phthalate on growth, lipid peroxidation and antioxidative enzyme activity in Dunaliella salina

The objective of this study was to investigate the effect of exogenous selenium (Se) supply (0, 2, 4, 8, 16 μM) on the growth, lipid peroxidation and antioxidative enzyme activity of 100 mM NaCl-stressed melon (<em>Cucumis melo</em> L.) seedlings. Salt stress significantly reduced the growth attributes including stem length, stem diameter, dry weight and increased antioxidative enzyme activity [superoxide dismutase (SOD), peroxidase (POD), catalase (CAT)]. Moreover, the plant exhibited a significant increase in electrolyte leakage and malondialdehyde (MDA) content under NaCl stress. Se supplementation not only improved the growth parameters but also successfully ameliorated the adverse effect caused by salt stress in melon seedlings. However, the mitigation of NaCl-stressed seedlings was different depending on the Se concentration. At lower concentrations (2–8 μM), Se improved growth and acted as antioxidant by inhibiting lipid peroxidation and increasing in SOD and POD enzymes activity under salt stress. At higher concentrations (16 μM), Se exerted diminished beneficial effects on growth. Whereas CAT activity was enhanced. The result indicated that Se supplementation had a positive physiological effect on the growth and development of salt-stressed melon seedlings.

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Transcriptome, physiological and biochemical analysis of Triarrhena sacchariflora in response to flooding stress

BMC Genetics ◽

10.1186/s12863-019-0790-4 ◽

2019 ◽

Vol 20 (1) ◽

Author(s):

Jia Wang ◽

Han Sun ◽

Jiajin Sheng ◽

Surong Jin ◽

Fasong Zhou ◽

...

Keyword(s):

Lipid Peroxidation ◽

Enzyme Activity ◽

Molecular Mechanisms ◽

Soluble Sugar ◽

Antioxidative Enzyme ◽

Pathway Enrichment Analysis ◽

Photosynthetic System ◽

Flooding Stress ◽

Antioxidative Enzyme Activity ◽

Physiological And Biochemical

Abstract Background In recent decades, the frequency of flooding is increasing with the change of global climate. Flooding has become one of the major abiotic stresses that seriously affect growth and development of plants. Triarrhena sacchariflora Nakai has been considered a promising energy crop for utilization in ethanol production. Flooding stress is among the most severe abiotic stressors in the production of Nakai. However, the physiological and molecular biological mechanisms of Nakai response to flooding is still unclear. In the present study, in order to understand the molecular mechanisms of Nakai in response to flooding stress, the transcriptome, physiological and biochemical were investigated. Results The results demonstrated that significant physiological changes were observed in photosynthetic system, antioxidative enzyme activity, chlorophyll, carotenoid, proline, lipid peroxidation and soluble sugar content under normal and flooding treatments. Such as, the chlorophyll, carotenoid contents and photosynthetic system were significantly decreased. Whereas, the antioxidative enzyme activity, proline, lipid peroxidation and soluble sugar has increased first and then decreased under treatments compared with the normal plants. Additionally, a total of 8832, 6608 and 3649 unigenes were validated to be differentially expressed under different treatments, respectively. Besides, gene ontology enrichment and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis of the different expression levels of genes also presented processes, which involved in photosynthesis, sucrose catabolism, glycolysis, stress response and defense, phytohormone biosynthesis and signal transduction. Conclusions The results provide a comprehensive view of the complex molecular events involved in the response to flooding stress of Nakai leaves, which also will promote the research in the development of flood-resistant crops and provide new tools for Nakai breeders.

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Plant Growth, Antioxidative Enzymes and Lipid Peroxidation in Sunflower Seedlings Supplemented With Eichhornia crassipes Organic Fertilizer Under Drought Stress Conditions

Journal of Agricultural Science ◽

10.5539/jas.v11n1p504 ◽

2018 ◽

Vol 11 (1) ◽

pp. 504

Author(s):

Rifandreo Monteiro Barbosa ◽

Paulo Ovídio Batista de Brito ◽

Francisca Raíssa da Silva Costa ◽

Roberto Albuquerque Pontes Filho ◽

Francisco Holanda Nunes Júnior ◽

...

Keyword(s):

Lipid Peroxidation ◽

Enzyme Activity ◽

Drought Stress ◽

Plant Growth ◽

Eichhornia Crassipes ◽

Organic Fertilizer ◽

Antioxidative Enzyme ◽

Stress Conditions ◽

Semiarid Region ◽

Antioxidative Enzyme Activity

In the semiarid region of the Brazilian Northeast, there is still the occurrence of soils with low concentrations of organic mass and nutrients. Eichhornia crassipes (water hyacinth) is recognized as one of the top ten endemic herbs in the world. However, its accumulation capacity means it can be an alternative source of nutrients. The objective of this study was to analyse the effects of macrophyte organic residue (ROM) on plant growth, antioxidative enzyme activity and membrane lipid peroxidation in leaves and roots of sunflower seedlings submitted to drought stress conditions. The experiment was conducted under greenhouse conditions at the Instituto Federal de Educação, Ciência e Tecnologia do Ceará, Maracanaú Campus, Brazil. Samples of E. crassipes were collected in the Parangaba Lagoon, Fortaleza, Ceará, Brazil. The treatments were: 1) 100% (by volume) sand; 2) sand + fertilizer (following the recommendation of 80 kg of N/ha), and 3) sand + 100% of the recommended nitrogen in macrophytes (RN). In general, the use of ROM caused better seedling growth in relation to the other treatments in all conditions studied. Increases in antioxidative enzyme activity and reductions in the deleterious effects of drought stress on plant growth were observed.

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