scholarly journals Comparison of three different rumen fluid as a source of inoculum to evaluate in vitro gas production and digestibility of elephant grass-concentrate mixture

2021 ◽  
Vol 888 (1) ◽  
pp. 012076
Author(s):  
H Soetanto ◽  
RM Aprilia ◽  
MS Pramita ◽  
I Banna
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Elephant Grass ◽  
Buffer Solution ◽  
Cattle Breeds ◽  
In Vitro Gas Production ◽  
Concentrate Mixture

Abstract This study aimed at elucidating the use of three different rumen fluid (RF) of indigenous cattle breeds i.e. Bali, Madura and Crossbred Ongole immediately after slaughtered at abattoir to evaluate the nutritive value of elephant grass( EG) -concentrate mixture using a standard in vitro gas production (IVGP) technique. Approximately 500 mg feed dry matter/syringe was added with 50 ml RF-buffer solution and incubated in a 39 0C water bath for 48 hours where gas production was observed at time intervals. Following termination of incubation the content was transferred into tare glass crucible to measure rumen dry matter (RDMD) and organic matter (ROMD) digestibility. The results showed that there was no significant different (P>0.05) in gas production parameters. In contrast, RDMD and ROMD differed significantly (P<0.01) among cattle breeds. RF from OCB resulted in the highest IVGP, RDMD and ROMD as compared with other RF sources. In conclusion, the use of RF from abattoir for IVGP measurement can be warranted using the same source of RF. The highest values resulted from OCB suggests that the abundance and variation in rumen microbiota may exist among cattle breeds.

Effect of the ratio of maize cob and husk to napier Pakchong 1 silage on nutritive value and in vitro gas production of rumen fluid of Thai native cattle

2017 ◽  
Vol 57 (8) ◽  
pp. 1603 ◽  
Author(s):  
S. Yammuen-art ◽  
P. Somrak ◽  
C. Phatsara
Keyword(s):  
Microbial Biomass ◽  
Nutritive Value ◽  
Biomass Yield ◽  
Rumen Fluid ◽  
Gas Production ◽  
Average Weight ◽  
In Vitro Gas Production ◽  
Native Cattle ◽  
Maize Cob

The present study evaluated the chemical composition and in vitro ruminal digestibility of napier Pakchong 1 silage combined with maize cob and husk in different ratios. The napier Pakchong 1 grass was harvested at 45 days of maturity. The napier Pakchong 1 grass was ensiled with maize cob and husk at ratios of 1:5, 1:10 and 1:15. Three rumen fistulated Thai native cattle (White Lamphun cattle) with an average weight of 154 ± 4.7 kg were used to determine ruminal digestibility by in vitro gas-production technique. Gas production was recorded after incubating for 2, 4, 8, 12, 24, 48, 72 and 96 h. The microbial biomass yield was determined after incubating for 24 h. DM, NDF and ADF of maize cob and husk mixed with napier Pakchong 1 silage declined by increasing the proportion of napier Pakchong 1 grass, while gas production after 4–10 h of incubating maize cob and husk mixed with napier Pakchong 1 grass increased by increasing the proportion of napier Pakchong 1 grass. The metabolisable energy, organic matter digestibility and microbial biomass yield did not differ among the different ratios. The results of the study suggested a recommended ratio of maize cob and husk to napier Pakchong 1 grass of 1:10. The ensiling fermentation increased the proportion of protein in the roughage, which lead to increased in vitro gas production of roughage.

scholarly journals Corrigendum to: Effect of the ratio of maize cob and husk to napier Pakchong 1 silage on nutritive value and in vitro gas production of rumen fluid of Thai native cattle

2019 ◽  
Vol 59 (3) ◽  
pp. 600
Author(s):  
S. Yammuen-art ◽  
P. Somrak ◽  
C. Phatsara
Keyword(s):  
Microbial Biomass ◽  
Nutritive Value ◽  
Biomass Yield ◽  
Rumen Fluid ◽  
Gas Production ◽  
Average Weight ◽  
In Vitro Gas Production ◽  
Native Cattle ◽  
Maize Cob

The present study evaluated the chemical composition and in vitro ruminal digestibility of napier Pakchong 1 silage combined with maize cob and husk in different ratios. The napier Pakchong 1 grass was harvested at 45 days of maturity. The napier Pakchong 1 grass was ensiled with maize cob and husk at ratios of 1:5, 1:10 and 1:15. Three rumen fistulated Thai native cattle (White Lamphun cattle) with an average weight of 154 ± 4.7 kg were used to determine ruminal digestibility by in vitro gas-production technique. Gas production was recorded after incubating for 2, 4, 8, 12, 24, 48, 72 and 96 h. The microbial biomass yield was determined after incubating for 24 h. DM, NDF and ADF of maize cob and husk mixed with napier Pakchong 1 silage declined by increasing the proportion of napier Pakchong 1 grass, while gas production after 4–10 h of incubating maize cob and husk mixed with napier Pakchong 1 grass increased by increasing the proportion of napier Pakchong 1 grass. The metabolisable energy, organic matter digestibility and microbial biomass yield did not differ among the different ratios. The results of the study suggested a recommended ratio of maize cob and husk to napier Pakchong 1 grass of 1:10. The ensiling fermentation increased the proportion of protein in the roughage, which lead to increased in vitro gas production of roughage.

scholarly journals Determination of in vitro rumen digestibility and potential feed value of tiger nut varieties

2021 ◽  
Vol 50 (5) ◽  
Author(s):  
T. Ayaşan ◽  
E. Sucu ◽  
I. Ülger ◽  
H. Hızlı ◽  
P. Cubukcu ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Nutritive Value ◽  
Energy Content ◽  
Rumen Fluid ◽  
Gas Production ◽  
Time Dependent ◽  
In Vivo Studies ◽  
In Vitro Digestibility ◽  
In Vitro Gas Production

Tiger nut (Cyperus esculentus L.), or chufa, is a plant that is found in nature and is cultivated for its edible tubers. The purpose of this study was to determine the chemical composition, nutritive value, and in vitro digestibility of three tiger nut varieties using the in vitro gas production technique. These varieties were Sarışeker (yellow), Introduction 1, and Balyumru (brown). Rumen fluid was obtained from two cannulated Holstein animals. Time-dependent in vitro gas production was monitored at 3, 6, 9, 12, 24, 48, 72, and 96 hours of incubation. The varieties differed in dry matter (DM), crude ash (CA), ether extract (EE), neutral detergent fibre (NDF), and non-fibre carbohydrate (NFC) content (P <0.05). They also differed in the instantaneous volume of gas produced and in time-dependent gas production. Balyumru produced more gas at the onset of incubation than Introduction 1 and Sarışeker. However, over time, the gas produced by digestion of Introduction 1 exceeded the other two varieties. The amounts of gas produced at each time-point were intercorrelated. It is recommended that these results should lead to further evaluation in in vivo studies. Keywords: chemical composition, energy content, in vitro gas production

Relationship between in vitro gas production and in vivo energy digestibility in growing pigs

1996 ◽  
Vol 1996 ◽  
pp. 142-142
Author(s):  
A.C. Longland ◽  
S.P. Bray ◽  
A.E. Brooks ◽  
M.K. Theodorou ◽  
A.G. Low
Keyword(s):  
Pressure Transducer ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Growing Pigs ◽  
Digestible Energy ◽  
In Vitro Gas Production

In vitro methods which can be used to predict the nutritive value of feedstuflfs for livestock are attractive in terms of both speed and economy. The in vitro pressure transducer technique (PTT) whereby the gas evolved during the in vitro fermentation of feedstuffs in rumen fluid is quantified by a pressure transducer, has been used to predict the nutritive value of ruminant feedstuffs. Here the potential for the PTT in predicting the digestible energy contents of eight feedstuffs varying in non-starch polysaccharide (NSP) content fed to growing pigs, was investigated.

Relationship between in vitro gas production and in vivo energy digestibility in growing pigs

1996 ◽  
Vol 1996 ◽  
pp. 142-142
Author(s):  
A.C. Longland ◽  
S.P. Bray ◽  
A.E. Brooks ◽  
M.K. Theodorou ◽  
A.G. Low
Keyword(s):  
Pressure Transducer ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Growing Pigs ◽  
Digestible Energy ◽  
In Vitro Gas Production

In vitro methods which can be used to predict the nutritive value of feedstuflfs for livestock are attractive in terms of both speed and economy. The in vitro pressure transducer technique (PTT) whereby the gas evolved during the in vitro fermentation of feedstuffs in rumen fluid is quantified by a pressure transducer, has been used to predict the nutritive value of ruminant feedstuffs. Here the potential for the PTT in predicting the digestible energy contents of eight feedstuffs varying in non-starch polysaccharide (NSP) content fed to growing pigs, was investigated.

Inclusion of yeast waste as a protein source to replace soybean meal in concentrate mixture on ruminal fermentation and gas kinetics using in vitro gas production technique

2019 ◽  
Vol 59 (9) ◽  
pp. 1682
Author(s):  
Anusorn Cherdthong ◽  
Rittikeard Prachumchai ◽  
Chanadol Supapong ◽  
Benjamad Khonkhaeng ◽  
Metha Wanapat ◽  
...  
Keyword(s):  
Soybean Meal ◽  
Dry Matter ◽  
Insoluble Fraction ◽  
Gas Production ◽  
Protein Source ◽  
Production Technique ◽  
Dry Matter Digestibility ◽  
In Vitro Gas Production ◽  
Concentrate Mixture

This experiment was conducted to investigate the utilisation of yeast waste as protein source to replace soybean meal in concentrate mixture on kinetic of gas, rumen ammonia-nitrogen and digestibility of nutrients by using in vitro gas production technique. The experimental design was a completely randomised design and the dietary treatments were replacing soybean meal with yeast wastein concentrate at the ratio of 100:0, 75:25, 50:50, 25:75 and 0:100, respectively. Yeast waste was obtained from KSL Green Innovation Public Co. Limited, Thailand. The gas production was recorded at 0, 0.5, 1, 2, 4, 6, 8, 12, 16, 24, 48, 72 and 96 h of incubation. The yeast waste contained 26.4% crude protein. Gas production from soluble fractions (a), gas production from the insoluble fraction (b), potential extent of gas production (a+b) and the gas production rate constants for the insoluble fraction (c) were not altered when increasing concentration of yeast waste replacing soybean meal (P &gt; 0.05). Cumulative gas production (at 96 h of incubation) ranged from 69.3 to 72.8 mL and was similar among treatments. Ruminal NH3-N concentration was linearly increased (P &lt; 0.05) whereas ruminal pH did not alter when inclusion various levels of yeast waste replacing soybean meal, which ranged from 15.2 to 19.1 mg/dL and 6.90 to 6.94, respectively. In vitro dry matter digestibility and in vitro organic matter digestibility did not changed by increasing levels of yeast waste in the diets (P &gt; 0.05), except only in vitro dry matter digestibility at 12 h, which higher in soybean meal:yeast waste at 25:75 ratio (P &lt; 0.05). Furthermore, propionate (C3) molar was linearly higher when compared between inclusion yeast waste and the control group whereas acetate was decreased quadratically (P &lt; 0.05) and protozoal population tended to be decreased (P = 0.07) when increasing the level of replacing yeast waste. In conclusion, yeast waste could replace soybean meal in concentrate mixture with no negative effect on gas kinetics, rumen fermentation and in vitro digestibility, and therefore its use in animal feeding would contribute to a reduction in environmental pollution.

Comparison of alfalfa and fenugreek (Trigonella foenum-graecum) silages supplemented with barley grain on performance of growing steers

1998 ◽  
Vol 78 (3) ◽  
pp. 343-349 ◽  
Author(s):  
Z. Mir ◽  
P. S. Mir ◽  
S. N. Acharya ◽  
M. S. Zaman ◽  
W. G. Taylor ◽  
...  
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Average Daily Gain ◽  
Gas Production ◽  
Dry Matter Intake ◽  
Plastic Tube ◽  
Alfalfa Silage

To compare nutritive value of alfalfa and fenugreek silages for weaned back-grounding feedlot steers, isonitrogenous diets of alfalfa or fenugreek silage, supplemented with three levels of steam-rolled barley (0, 15 and 30% DM) were fed ad libitum to 60 steers (initial weight of 237 ± 18 kg). The study was conducted in individual pens for 105 d using a 2 × 3 factorial experiment. Fenugreek was cut 17 wk after seeding while the alfalfa was cut at mid-bloom. Both fenugreek and alfalfa silages were prepared in plastic tube silos after wilting to 38 and 35% DM, respectively. After ensiling for 40 d, silage tubes were opened and a feeding trial was conducted. Alfalfa and fenugreek silage contained similar levels of CP (18.0 and 17.2%, respectively) while fenugreek silage contained a lower level of NDF (38.2%) than alfalfa silage (40.4%). Dry matter intake (DMI) and average daily gain (ADG) of steers increased (P < 0.001) and feed efficiency (FE) improved linearly (P < 0.001) with increasing grain level in the diet. Dry matter intake (6.4 vs. 6.2 kg d−1), ADG (0.82 vs. 0.84 kg d−1), FE (8.1 vs. 7.8), total rumen volatile fatty acids (103.4 vs. 105.3 mM), rumen fluid pH (6.9 vs. 6.9) and rumen ammonia concentration (9.6 vs. 10.4 mg dL−1) in steers were similar for both alfalfa- and fenugreek-silage-based diets, respectively. Although in vitro rate of gas production was different at 12.6 and 17.4% h−1 for alfalfa and fenugreek diets, respectively, potential gas production at the end of 72 h of fermentation was similar (21.6 vs. 22.3 mL 100 mg−1 DM). The presence of steroidal saponins in fenugreek plants did not affect steer performance or hormonal status when fed as silage. Results from this study indicate that the nutritive value of fenugreek silage is comparable to that of mid-bloom alfalfa silage, and that fenugreek, a high-DM yielding annual legume, has potential to be a valuable forage in feedlot diets. Key words: Alfalfa silage, fenugreek silage, annual legume, feedlot, steer, barley

scholarly journals PSVII-10 Evaluation of different biochar sources added at two inclusion levels in a grass hay- based diet on dry matter disappearance and ruminal fermentation parameters in vitro

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 296-296
Author(s):  
Genet Mengistu ◽  
Tim A McAllister ◽  
Kim Ominski ◽  
Gabriel O Ribeiro ◽  
Erasmus Okine ◽  
...  
Keyword(s):  
Dry Matter ◽  
Rumen Fluid ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Buffer Solution ◽  
N Concentration ◽  
Grass Hay ◽  
Inclusion Level ◽  
The Impact

Abstract This study evaluated the impact of adding biochar to a grass hay-based diet on in vitro dry matter disappearance (DMD), total gas production (GP), methane production (CH4), volatile fatty acid (VFA) and ammonia nitrogen (NH3-N) concentration. Treatments were arranged in a factorial design with 7 biochar products differing in origin (pine or coconut-based) and physical properties (course, fine or loose spheres) at 2 inclusion levels (2.25% and 4.5% of DM) and a grass hay-only control. On each three successive weeks, rumen fluid was collected from 2 ruminally cannulated beef heifers fed a grass hay-based diet, pooled and mixed with a buffer solution (1:2) and treatments incubated in triplicate for 48 h. Gas pressure was recorded at 3, 6, 9, 12, 18, 24, 36 and 48 h of incubation, and gas was collected at each time to measure CH4. At 48 h, pH was measured and the supernatant sampled for determination of VFA and NH3-N, while the residue was used to estimate DMD. Dry matter disappearance decreased (P &lt; 0.05) with increasing addition of biochar. Biochar did not affect GP or CH4, but did reduce (P &lt; 0.05) gas production per g of DM incubated (111.7 vs 107.8 ml/g), but not per g DMD. The lower GP per g DM incubated could be related to the increased proportion of the diet as biochar, without it contributing to GP. Biochar, irrespective of level did not affect the total or molar proportions of VFA. There was a biochar x inclusion level interaction (P &lt; 0.04) in NH3-N concentration. Biochar type and inclusion level in the present study had little or no significant effect on in vitro fermentation and did not mitigate CH4 production from a grass hay-based diet.

EVALUASI KECERNAAN RUMPUT KUMPAI MINYAK (Hymenachne amplexicaulis) AMONIASI SECARA IN VITRO

2013 ◽  
Vol 14 (1) ◽  
Author(s):  
Sindu Akhadiarto ◽  
A Fariani
Keyword(s):  
Organic Matter ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Poultry Manure ◽  
Gas Production ◽  
Buffer Solution ◽  
In Vitro Techniques ◽  
Dry Matter Digestibility ◽  
Organic Matter Digestibility

The objective of this research was to study digestibility of kumpai minyak (Hymenachne amplexicaulis) amoniation by in vitro techniques. Materials used in this study were : kumpai oil grass; poultry manure; urea; cattle rumen fluid; Mc buffer solution Dougall; NaOH or H3PO4 and saturated HgCl2. This research was held in two stage, the first was amoniation of kumpai minyak and second was in vitro analysis, both experiment was held at laboratory of Nutrition and Feed Animal, Agriculture Faculty at Sriwijaya University. The research used completely randomized design with 4 treatments and 4 replications which were: A0 (kumpai oil grass without urea), A1 (kumpai oil grass with urea 2%), A2 (kumpai oil grass with urea 4%) and A3 (kumpai oil grass with urea 6%). Parameters measuredwere dry matter digestibility, organic matter digestibility and gas production.. The result showed that the treatment were significantly difference (P<0.01) on organic matter digestibility, but non sinificantly differences (P>0.01) on dry matter digestibility and gas production.

Sign in / Sign up

Export Citation Format

Share Document