Comparison of alfalfa and fenugreek (Trigonella foenum-graecum) silages supplemented with barley grain on performance of growing steers

1998 ◽  
Vol 78 (3) ◽  
pp. 343-349 ◽  
Author(s):  
Z. Mir ◽  
P. S. Mir ◽  
S. N. Acharya ◽  
M. S. Zaman ◽  
W. G. Taylor ◽  
...  
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Average Daily Gain ◽  
Gas Production ◽  
Dry Matter Intake ◽  
Plastic Tube ◽  
Alfalfa Silage

To compare nutritive value of alfalfa and fenugreek silages for weaned back-grounding feedlot steers, isonitrogenous diets of alfalfa or fenugreek silage, supplemented with three levels of steam-rolled barley (0, 15 and 30% DM) were fed ad libitum to 60 steers (initial weight of 237 ± 18 kg). The study was conducted in individual pens for 105 d using a 2 × 3 factorial experiment. Fenugreek was cut 17 wk after seeding while the alfalfa was cut at mid-bloom. Both fenugreek and alfalfa silages were prepared in plastic tube silos after wilting to 38 and 35% DM, respectively. After ensiling for 40 d, silage tubes were opened and a feeding trial was conducted. Alfalfa and fenugreek silage contained similar levels of CP (18.0 and 17.2%, respectively) while fenugreek silage contained a lower level of NDF (38.2%) than alfalfa silage (40.4%). Dry matter intake (DMI) and average daily gain (ADG) of steers increased (P < 0.001) and feed efficiency (FE) improved linearly (P < 0.001) with increasing grain level in the diet. Dry matter intake (6.4 vs. 6.2 kg d−1), ADG (0.82 vs. 0.84 kg d−1), FE (8.1 vs. 7.8), total rumen volatile fatty acids (103.4 vs. 105.3 mM), rumen fluid pH (6.9 vs. 6.9) and rumen ammonia concentration (9.6 vs. 10.4 mg dL−1) in steers were similar for both alfalfa- and fenugreek-silage-based diets, respectively. Although in vitro rate of gas production was different at 12.6 and 17.4% h−1 for alfalfa and fenugreek diets, respectively, potential gas production at the end of 72 h of fermentation was similar (21.6 vs. 22.3 mL 100 mg−1 DM). The presence of steroidal saponins in fenugreek plants did not affect steer performance or hormonal status when fed as silage. Results from this study indicate that the nutritive value of fenugreek silage is comparable to that of mid-bloom alfalfa silage, and that fenugreek, a high-DM yielding annual legume, has potential to be a valuable forage in feedlot diets. Key words: Alfalfa silage, fenugreek silage, annual legume, feedlot, steer, barley

scholarly journals Comparison of three different rumen fluid as a source of inoculum to evaluate in vitro gas production and digestibility of elephant grass-concentrate mixture

2021 ◽  
Vol 888 (1) ◽  
pp. 012076
Author(s):  
H Soetanto ◽  
RM Aprilia ◽  
MS Pramita ◽  
I Banna
Keyword(s):  
Dry Matter ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Gas Production ◽  
Elephant Grass ◽  
Buffer Solution ◽  
Cattle Breeds ◽  
In Vitro Gas Production ◽  
Concentrate Mixture

Abstract This study aimed at elucidating the use of three different rumen fluid (RF) of indigenous cattle breeds i.e. Bali, Madura and Crossbred Ongole immediately after slaughtered at abattoir to evaluate the nutritive value of elephant grass( EG) -concentrate mixture using a standard in vitro gas production (IVGP) technique. Approximately 500 mg feed dry matter/syringe was added with 50 ml RF-buffer solution and incubated in a 39 0C water bath for 48 hours where gas production was observed at time intervals. Following termination of incubation the content was transferred into tare glass crucible to measure rumen dry matter (RDMD) and organic matter (ROMD) digestibility. The results showed that there was no significant different (P>0.05) in gas production parameters. In contrast, RDMD and ROMD differed significantly (P<0.01) among cattle breeds. RF from OCB resulted in the highest IVGP, RDMD and ROMD as compared with other RF sources. In conclusion, the use of RF from abattoir for IVGP measurement can be warranted using the same source of RF. The highest values resulted from OCB suggests that the abundance and variation in rumen microbiota may exist among cattle breeds.

scholarly journals Screening of Cyanide-Utilizing Bacteria from Rumen and In Vitro Evaluation of Fresh Cassava Root Utilization with Pellet Containing High Sulfur Diet

2020 ◽  
Author(s):  
Rittikeard Prachumchai ◽  
Anusorn Cherdthong ◽  
Metha Wanapat
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
In Vitro Study ◽  
Gas Production ◽  
Ruminal Fermentation ◽  
Cassava Root ◽  
Randomized Design ◽  
Completely Randomized Design

The current work aimed to screen the ruminal cyanide-utilizing bacteria and evaluate the influence of fresh cassava root (FCR) and pellets containing high sulfur (PELFUR) on cyanide content, kinetics of gas, in vitro degradability, and ruminal fermentation. The experiment was conducted in a Completely randomized design (CRD) for a screening of cyanide-utilizing bacteria and the dietary treatments were the level of cyanide at 0, 150, 300, and 450 ppm. A 5 &times; 3 factorial arrangement in a Completely randomized design was used for in vitro study. Factor A was the level of FCR at 0, 260, 350, 440, and 530 g/kg of 0.5 g dry matter (DM) substrate, and factor B was the level of PELFUR at 0, 15, and 30 g/kg DM substrate. Adding different doses of cyanide significantly affected cyanide-utilizing rumen bacterial growth (p &lt; 0.05). Increasing the concentration of cyanide from 0 to 150 and 150 to 300 ppm, resulted in an increase in cyanide-utilizing rumen bacteria of 38.2% and 15.0%, respectively. Increasing the FCR level to more than 260 g/kg of 0.5 g substrate could increase cumulative gas production (p &lt; 0.05), whereas increasing doses of PELFUR from 15 to 30 g/kg increased the cumulative gas production when compared with that of 0 g/kg PELFUR (p &lt; 0.05). Cyanide concentration in rumen fluid decreased with PELFUR (p &lt; 0.05) supplementation. Degradability of in vitro dry matter and organic matter following incubation increased at 12 and 24 h due to PELFUR supplementation with FCR and increased additionally with 15 g/kg PELFUR (p &lt; 0.05) in 440 g/kg FCR. Proportions of the total volatile fatty acids, acetic acid (C2), propionic acid (C3), and butyric acid, as well as the ratio of C2 to C3 among supplementations with FCR (p &lt; 0.05) were significantly different. As the proportion of FCR increased to 530 g/kg of the substrate, the volume of C3 increased by 14.6%. This is the first finding of bacteria in the rumen capable of utilizing cyanide, and cyanide might function as a nitrogen source for bacterial cell synthesis. Inclusion of FCR of 530 g/kg with 30 g/kg PELFUR could increase the cumulative gas production, the bacterial population, the in vitro degradability, the proportion of C3, and the rate of the disappearance of cyanide.

scholarly journals Effects of addition of exogenous fibrolytic enzymes on digestibility and milk and meat production – a systematic review

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Deli Nazmín Tirado-González ◽  
Gustavo Tirado-Estrada ◽  
Luis Alberto Miranda-Romero ◽  
Rodolfo Ramírez-Valverde ◽  
Sergio Ernesto Medina-Cuéllar ◽  
...  
Keyword(s):  
Systematic Review ◽  
Milk Yield ◽  
Dry Matter ◽  
Production Efficiency ◽  
Volatile Fatty Acids ◽  
Average Daily Gain ◽  
Gas Production ◽  
Meat Production ◽  
Fibrolytic Enzymes

Abstract Exogenous fibrolytic enzymes (EFE) added to the ruminant diet can increase fiber digestibility and production efficiency. A systematic review was conducted to understand the interactions between EFE and diet on digestibility and animal performance. The database included variables from 384 experiments with EFE and 264 controls from 85 papers published since 2000 (classification criteria: 1) type of study (in vitro, in situ, in vivo), 2) type of ruminants (sheep, Buffaloes, goats, beef and dairy cattle), 3) primary EFE activity (Cellulases (Cel) or Xylanases (Xyl)), 4) forage proportion (FP), 5) type of plant (TP: legumes or grasses), 6) number of ingredients in diets, and 7) application time (AT)). In over of 52.85% of cases, EFE improved the degradability of dry matter (DMD), neutral and acid detergent fiber (NDFD and ADFD), in vitro gas production (GP), volatile fatty acids (VFA), the acetate: propionate ratio (A:P ratio), protein and fat milk, milk yield and average daily gain (ADG) (by 7.78–21.85%). Cel improved organic matter degradability (OMD), GP, VFA, milk yield, and milk protein and fat content. EFE in FP≥40% diets enhanced the ADG, and in grass-based diets increased the dry matter intake (DMI). The AT of EFE affected the DMD, NDFD, and ADFD. Significant correlations were found between the improvements of NDFD or ADFD with DMD (r>0.59), milk yield (r=0.64), and ADG (r=0.59). In conclusion, many factors interact with EFE supplementation effects, but EFE consistently enhanced the DMD, NDFD, and ADFD of ruminant diets, which are related to improvements in productive performance.

NUTRITIVE VALUE OF SEDGE AND KENTUCKY BLUEGRASS HAYS GROWN IN ALBERTA

1977 ◽  
Vol 57 (1) ◽  
pp. 187-193 ◽  
Author(s):  
G. M. J. HORTON ◽  
L. W. McELROY
Keyword(s):  
Trichloroacetic Acid ◽  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
In Vitro Digestion ◽  
Kentucky Bluegrass ◽  
Lake Bottom ◽  
Oat Straw

The nutritive values of sedge and Kentucky bluegrass hays grown in a partially drained lake bottom in central Alberta were assessed in the laboratory and compared with those of brome–fescue hay and oat straw. The extent and rate of in vitro digestion for each forage with rumen fluid from cows fed either sedge, Kentucky bluegrass or brome–fescue were measured using a two-stage technique. The forages were analyzed for proximate components and minerals, and the rumen fluid for trichloroacetic acid-insoluble nitrogen (TCA-N) and volatile fatty acids (VFA). The effects of sulphur and phosphorus supplementation on the in vitro digestion of purified cellulose in the presence of aqueous extracts of sedge, Kentucky bluegrass and brome–fescue were also determined. In vitro dry matter and cellulose digestibilities were highest for brome–fescue and lowest for oat straw; DMD values for Kentucky bluegrass were consistently higher (P > 0.05) than for sedge. Phosphorus supplementation did not enhance cellulose digestion with brome–fescue extract, but with sedge and Kentucky bluegrass extracts, the responses were significant. Ruminal TCA-N and VFA concentrations were highest after feeding brome–fescue, followed by Kentucky bluegrass and then sedge. These studies indicated that the nutritive value of brome–fescue hay was highest, followed by Kentucky bluegrass and sedge hays, and then oat straw.

scholarly journals Effect of Maize Stover in Fermented Total Mixed Ration on Nutritive Value and Digestibility Quality in Vitro

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
R. Buddanoi ◽  
A. Abrar ◽  
C. Wachirapakorn
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Gas Production ◽  
Control Group ◽  
Maize Stover ◽  
Total Mixed Ration ◽  
Dry Matter Digestibility ◽  
In Vitro Technique

The objective of this experiment was to investigate the effect of maize stover used as roughage source in fermented total mixed ration on nutritive value and digestibility quality by in vitro technique. The experimental compared between the feeds non-fermented total mixed ratio (TMR) group and fermented total mixed ratio (FTMR) group by fermentation 21 days, each group were ratio 60:40 and five replications. The results revealed that CF content of FTMR increased from 33.79% to 40.70% significantly different (P<0.05) and DM, CP, EE and ash were not significantly different between treatments (P>0.05). The digestibility quality were dry matter digestibility (%DMD) in FTMR higher than control group significantly different (P<0.05). Moreover total volatile fatty acids (VFAs) in FTMR increased from 15.00 to 39.50 mM higher than control group not significantly different (P>0.05) and N-NH3 concentrations were not significantly different (P>0.05). The gas production was recorded at 0, 6, 12, 18, and 24h of incubation. The cumulative gas production curve both of treatments was remarkable boost from 0h to 6h incubation of FTMR from 57.5 to 37 ml and then incubation time the cumulative gas production both of them were slowly decrease.

scholarly journals Preserving Porphyra umbilicalis and Saccharina latissima as Silages for Ruminant Feeding

Animals ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1957
Author(s):  
Margarita Novoa-Garrido ◽  
Carlos Navarro Marcos ◽  
María Dolores Carro Travieso ◽  
Eduarda Molina Alcaide ◽  
Mogens Larsen ◽  
...  
Keyword(s):  
Dry Matter ◽  
Nutritional Value ◽  
Volatile Fatty Acids ◽  
Gas Production ◽  
Saccharina Latissima ◽  
Porphyra Umbilicalis ◽  
In Vitro Gas Production ◽  
Ruminal Degradability ◽  
Kinetics Of

The study analyzed the characteristics, chemical composition, and in vitro gas production kinetics of Porphyra umbilicalis and Saccharina latissima silages. Each seaweed was ensiled in vacuum bags (three bags/silage) following a 2 × 3 factorial design, with two pre-treatments (unwilted or pre-wilted) and three silage types: unwashed seaweed ensiled without additive; seaweed washed and ensiled without additive; and seaweed washed and ensiled with 4 g of formic acid (FAC) per kg seaweed. Silages were kept for 3 months in darkness at 20 °C. Pre-wilting prevented (p < 0.001) effluent formation and reduced (p ≤ 0.038) the production of NH3-N and volatile fatty acids for both seaweeds. Both pre-wilting and washing increased (p < 0.05) the ruminal degradability of P. umbilicalis silages but not of S. latissima silages. The pH of the FAC-treated silages was below 4.0, but ranged from 4.54 to 6.23 in non FAC-treated silages. DL-lactate concentrations were low (≤23.0 g/kg dry matter) and acetate was the predominant fermentation product, indicating a non-lactic fermentation. The estimated ruminal degradability of the P. umbilicalis and S. latissima silages was as average, 59.9 and 86.1% of that for high-quality rye-grass silages, respectively, indicating a medium-low nutritional value of these seaweed silages for ruminants.

scholarly journals The nutritional evaluation of forage-based mixed rations in New Zealand using an in vitro gas production technique. 1: analytical survey

2021 ◽  
pp. 1-6
Author(s):  
N.D. Meads ◽  
R. Tahmasbi ◽  
N. Jantasila
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Environmental Science ◽  
Ghg Emissions ◽  
Negative Relationship ◽  
Real Data ◽  
Gas Production ◽  
Production Technique ◽  
In Vitro Gas Production

Greenhouse gas (GHG) emissions from livestock are an important consideration in environmental science. Estimating GHG production can be problematic at a farm or animal level, and requires controlled conditions to produce real data. An in vitro gas production technique (IVGPT) was developed to evaluate forage-based total mixed rations in digestion kinetics and GHG production. Two hundred and sixty samples of complete mixed rations (MR), which included a pasture component used in commercial lactating dairy herds, were collected around NZ across three calendar years, 2017-2019. Twenty of the 260 samples were 100% total mixed rations (TMR) with no pasture content. The samples were submitted for proximate analysis as well as IVGPT to generate GHG production figures. The results showed an average total gas production (TGP) of 129.82 ml/g dry matter (DM), 78.6% true digestibility (TDMD), 125.06 mg/g DM microbial biomass (MB), 20.16 g CH4/kg DM, and 12.8 MJME/kg DM. The average nutrient composition was dry matter (DM) 31.55%, crude protein (CP) 21.85%, neutral detergent fibre (NDF) 44.35%, and starch 7.03%. The IVGPT CH4 production was negatively correlated to NDF (r=-0.312), ADF (r=-0.193), TGP (r=-0.216), and was positively correlated with TDMD (r=0.250), apparent digestibility (ADMD) (r=0.614), starch (r=0.117) and volatile fatty acids (r=0.538). The MR diet showed a strong positive relationship with ADMD digestibility (P=0.01) and a negative relationship with fibre content (NDF, P=0.01 and ADF, P=0.01). However, CH4 production reduced linearly with increasing TGP (P=0.01). The results indicated that a greater CH4 production may be related to higher digestibility of mixed ration.

scholarly journals Produk Fermentasi Rumen dan Produksi Protein Mikroba Sapi Lokal yang Diberi Pakan Jerami Amoniasi dan Beberapa Bahan Pakan Sumber Energi

2011 ◽  
Vol 11 (2) ◽  
pp. 29-34 ◽  
Author(s):  
Novita Hindratiningrum ◽  
Muhamad Bata ◽  
Setya Agus Santosa
Keyword(s):  
Protein Synthesis ◽  
Rice Bran ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Gas Production ◽  
Metabolic Energy ◽  
Energy Sources ◽  
Microbial Protein ◽  
Microbial Protein Synthesis

Products of rumen fermentation and protein microbial of dairy cattle feed with rice bran ammonization and some feedstuffs as an energy sourcesABSTRACT. This study aims to examine the energy sources of feed ingredients that can increase the production of Volatile Fatty Acids (VFA), N-NH3, microbial protein synthesis, total gas production and metabolic energy. The material used is as a source of rumen fluid inoculum from Frisian Holstein cows (FH) females, amoniasi rice straw, salt, mineral mix brand "Ultra Minerals' production Eka Farma Semarang, onggok wet and dry, corn, and rice bran. Observed variable is the concentration of (VFA), N-NH3, rumen microbial protein synthesis, and total gas production. Based on the analysis of diversity seen any significant effect (P0.05) on total VFA concentration, N-NH3 and total gas but had no effect (P0.05) on microbial protein synthesis. Conclusion of research is the provision of energy sources with rice bran treatment, onggok wet and dry corn flour can be used as fermentable carbohydrates on feed hay amoniasi in vitro.

scholarly journals PSVI-42 Effect of increasing concentrations of aflatoxins (AFB1) on rumen gas production (GP), dry matter (DM) disappearance and fermentation parameters using the in vitro ANKOM gas technique

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 194-195
Author(s):  
Jean-Philippe Marden ◽  
Virginie Marquis ◽  
Kheira Hadjeba Medjdoub ◽  
Marine Lacombe
Keyword(s):  
Feed Efficiency ◽  
Dry Matter ◽  
In Vitro Model ◽  
Rumen Fluid ◽  
Gas Production ◽  
Corn Silage ◽  
Vitro Model ◽  
Fermentation Parameters ◽  
Cheese Cloth

Abstract Aflatoxins are secondary metabolites produced by Aspergillus species known to be the most prevalent contaminants in feedstuffs. In ruminants, contaminated AFB1 feeds usually exhibit symptoms including reduced feed efficiency and milk production and decreased appetite. The objective of this study was to investigate the effects of different concentrations of AFB1 on rumen fermentation parameters by using the ANKOM gas production protocol. Rumen fluid was collected from a cannulated dry dairy cow, filtered with cheese-cloth and diluted (1:1) with a standard buffer. Triplicates of 75 mL flasks were fed 0,75g of feed (79% corn silage, 15% alfalfa and 6% concentrates) and inoculated with 0 (blank), 0,2, 0,5, 1 and 2 ppm of AFB1. Flasks were placed in a rotating incubation at 39°C for 96h and connected to ANKOM GP system. After 96h of incubation, the contents of each flask were centrifuged. Supernatants were analyzed for total VFA and AFB1 while precipitates were dried at 104°C for DM disappearance. The experimentation was repeated weekly 3 times and named wk1, 2 and 3. Statistical analysis was done by SPSS using a univariate model. Results showed no significant differences on GP max at 96h among AFB1 concentrations. Only wk 1 showed that higher AFB1 concentration (2 ppm) decreased significantly (P &lt; 0,05) DM disappearance (- 8,2 pts) when compared to the blank. Total VFA contents (75,0 ± 1,6 mM) were not affected by AFB1. Wk 2 and 3 did not show any difference neither on DM disappearance nor on VFA (89,1 ± 1,6 mM; 110,2 ± 4,8 mM). It can be concluded that our in vitro model, GP did not reflect DM disappearance and it can be put forward that rumen fluid with low total VFA concentrations (≤ 75 mM) could be more sensible to AFB1 challenge.

NUTRITIVE VALUE OF FORMALDEHYDE-TREATED RAPESEED MEAL FOR DAIRY CALVES

1972 ◽  
Vol 52 (2) ◽  
pp. 363-371 ◽  
Author(s):  
H. R. SHARMA ◽  
J. R. INGALLS ◽  
J. A. McKIRDY
Keyword(s):  
Dry Matter ◽  
Volatile Fatty Acids ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Protein Digestibility ◽  
Rapeseed Meal ◽  
Dairy Calves ◽  
Plasma Urea ◽  
Significant Difference ◽  
Daily Gain

Treatment of oilseed meals with formaldehyde (FA) and glutaraldehyde (GA) significantly (P < 0.01) reduced the solubility of protein in 0.02 N NaOH and the production of NH3 in the artificial rumen. Ten Holstein and Holstein × Brown Swiss calves of both sexes were fed two test rations containing either 26% untreated or formaldehyde-treated (5.6 g FA per 100 g protein) rapeseed meal (FA-treated RSM) for 14 weeks. Plasma urea nitrogen (N) and rumen NH3 levels were significantly (P < 0.01) lower in the FA-treated RSM calves compared with the control calves during the first 8 weeks of the growth trial. No significant difference (P > 0.05) in dry matter consumption, daily gain, or feed efficiency was observed. Total volatile fatty acids (VFA) (mmoles/100 ml) concentration was significantly higher (P < 0.05) in rumen fluid from the control calves compared with those receiving treated RSM. Molar percentages of the VFA’s were not significantly different (P > 0.05) between the two groups. In the N balance and digestibility trial, dry matter consumption did not differ significantly (P > 0.05); however, the FA-treated RSM resulted in decreased (P < 0.05) dry matter and crude protein digestibility. Calves receiving the treated RSM had reduced (P < 0.01) urinary N excretion; however, there was no significant effect (P > 0.05) on N consumption and retention.

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