scholarly journals Bioactive materials in the traumatology of small pets

2021 ◽  
Vol 937 (2) ◽  
pp. 022016
Author(s):  
O Krotova ◽  
N Bashkatova ◽  
K Savenkov ◽  
S Sklyarov ◽  
A Manjikova
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Fractures ◽  
Control Group ◽  
Bioactive Materials ◽  
Primary Bone ◽  
Synthetic Hydroxyapatite ◽  
New Generation

Abstract The effect of the biocomposition material was studied on the basis of synthetic hydroxyapatite, collagen and antibiotic for reparative histogenesis of bone tissue during shoulder bone fractures and forearm in dogs and implants for the tissue regeneration of the new generation Xenograft Collagen.It has been established that remodeling of the primary bone corn into the secondary in the region of the fracture in the dogs studied was diverse. During the experiment, it was revealed that a month after the operation on radiographs in the control group of dogs, there was a defect in which the formation of a weak periosal bone corn was observed. Endostal bone corn has not been detected. The most pronounced process proceeded by dogs in experienced groups. In the first experimental defect, a collapan was filled with a drug, and in the second used the implant in the form of the xenograft collagen granules. In animals of the first experienced group, the primary bone-cartilage corn had a more mature and developed character, intermediary and periosal corns were formed.

scholarly journals Biofabrication of SDF-1 Functionalized 3D-Printed Cell-Free Scaffolds for Bone Tissue Regeneration

2020 ◽  
Vol 21 (6) ◽  
pp. 2175 ◽  
Author(s):  
Alina Lauer ◽  
Philipp Wolf ◽  
Dorothea Mehler ◽  
Hermann Götz ◽  
Mehmet Rüzgar ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Growth ◽  
Collagen Type ◽  
Biomechanical Testing ◽  
Collagen Type I ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Type I

Large segmental bone defects occurring after trauma, bone tumors, infections or revision surgeries are a challenge for surgeons. The aim of our study was to develop a new biomaterial utilizing simple and cheap 3D-printing techniques. A porous polylactide (PLA) cylinder was printed and functionalized with stromal-derived factor 1 (SDF-1) or bone morphogenetic protein 7 (BMP-7) immobilized in collagen type I. Biomechanical testing proved biomechanical stability and the scaffolds were implanted into a 6 mm critical size defect in rat femur. Bone growth was observed via x-ray and after 8 weeks, bone regeneration was analyzed with µCT and histological staining methods. Development of non-unions was detected in the control group with no implant. Implantation of PLA cylinder alone resulted in a slight but not significant osteoconductive effect, which was more pronounced in the group where the PLA cylinder was loaded with collagen type I. Addition of SDF-1 resulted in an osteoinductive effect, with stronger new bone formation. BMP-7 treatment showed the most distinct effect on bone regeneration. However, histological analyses revealed that newly formed bone in the BMP-7 group displayed a holey structure. Our results confirm the osteoinductive character of this 3D-biofabricated cell-free new biomaterial and raise new options for its application in bone tissue regeneration.

scholarly journals Mesoporous bioactive glasses: Relevance of their porous structure compared to that of classical bioglasses

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Isabel Izquierdo-Barba ◽  
María Vallet-Regí
Keyword(s):  
Bone Tissue ◽  
Nanostructured Materials ◽  
Tissue Regeneration ◽  
Sol Gel ◽  
Added Value ◽  
Bone Tissue Regeneration ◽  
Chemical Compositions ◽  
Bioactive Glasses ◽  
Gel Glasses ◽  
New Generation

AbstractIn the last decade, the development of third generation bioceramics for Bone Tissue Regeneration has experienced significant progress with the emergence of a new generation of nanostructured materials named mesoporous bioactive glasses (MBG). This new generation of materials, also known as “templated glasses”, presents chemical compositions similar to those of conventional bioactive sol–gel glasses and the added value of an ordered mesopore arrangement. This article shows an indepth comparative study of the ordered porous structures of MBGs compared to conventional glasses (melt and solgel) andhowthese properties influence the bioactivity process. Moreover, the possibility to tailor the textural and structural properties of these nanostructured materials by an exhaustive control of the different synthesis parameters is also discussed. A brief overview regarding the possibility of using these materials as controlled drug delivery systems and as starting materials for the fabrication of 3D scaffolds for bone tissue regeneration is also given.

scholarly journals Morphological and radiological characteristics of experimental bone tissue regeneration

2021 ◽  
pp. 94-104
Author(s):  
E. A. Nadyrov ◽  
V. I. Nikolaev ◽  
S. I. Kirilenko ◽  
V. V. Rozhin ◽  
N. G. Maltseva ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Experimental Animals ◽  
Male Wistar Rats ◽  
Group A ◽  
Bone Trabeculae ◽  
The Right ◽  
Experimental Group

Objective. To study the dynamics of reparative bone tissue regeneration in experimental animals using a native transplantation autosmix (NTA).Materials and methods. Male Wistar rats weighing 180–200 g and aged 6–7 months were used. Bone tissue defects were modelled using a cutter with a diameter of 2 mm (experimental group). A similar defect of the middle third of the right tibia was formed in the control group animals without flling the defects with bone tissue. The animals were removed from the experiment on days 3, 7, 14, 30 and 60 of the experiment (per 6 animals for each observation period). The indicators of defect occupancy, necrosis, the area of bone trabeculae, the area of granulation tissue were determined in the histological sections. All the indicators were presented as percentage of the tested area. Osteoblasts, osteocytes, and osteoclasts were calculated in absolute values on the tested area.Results. The study has showed a higher rate of bone tissue formation in the bone defects in the experimental animals after autotransplantation of the bone mixture. Bone recovery in the experimental group had a higher rate, which was manifested by faster flling of the bone tissue defect, a low percentage of the necrosis area, a higher area of bone trabeculae, its rapid transformation into lamellar bone tissue.Conclusion. Osteogenesis had practically completed by day 30 of the experiment. At the same time, in the control group it had completed by day 60. The results obtained are a theoretical precondition for further research in the feld of bone autoplasty. Advanced development of surgical and minimally invasive technologies of bone mixture application will increase the effciency of modern reconstructive surgery of bones and joints.

EVALUATION OF BONE TISSUE REGENERATION USING A COMBINATION OF AUTOLOGOUS FIBRIN MATRIX WITH PRE-DIFFERENTIATED MESENCHYMAL STEM CELLS IN AN EXPERIMENTAL MODEL

2019 ◽  
Vol 25 (3) ◽  
pp. 172-175
Author(s):  
Igor S. Kopetsky ◽  
V. V. Loginov ◽  
S. D. Ryzhova ◽  
P. S. Virgilyev ◽  
A. V. Lokhonina ◽  
...  
Keyword(s):  
Stem Cells ◽  
Experimental Study ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Damage Zone ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Facial Skeleton ◽  
Fibrin Matrix

Introduction. According to domestic and foreign authors, the frequency of damage to the mandible bone is 36-84% of all damage to the bones of the facial skeleton. Since A.Y. Friedenstein made a key contribution to the study of mesenchymal stem cells (MSC), their analysis, types, methods of isolation and use in various combinations with materials, allowed to expand the boundaries of regenerative medicine. Aim of the study. The purpose of the study is to evaluate the regenerative potential of cortical bone tissue in the region of the mandible angle, with the introduction of exogenous pre-differentiated MSC (MSCs) on the autologous fibrin matrix in the experiment. Material and methods. We carried out an experimental study on 27 rabbits of the Soviet Chinchilla breed. Animals were divided into 3 groups: A, B and control. The bone defect diameter of 6mm was formed in the angle of the mandible of all animals with a trepan. Groups A and B mandible defects were injected with autologous fibrin clot with pre-differentiated MSCs in the amount of 300 thousand and 1 million cells, respectively. Defect size was assessed after 8, 10 and 12 weeks. Results. At week 12 after surgery, in the experimental group B the size of the bone tissue defect was significantly reduced and amounted to 0.006022 ± 0.00135 mm3, while in the control group the defect volume was 0.05597 ± 0.00505 mm3, and in group A was 0.02235 ± 0.0056 mm3. In an experimental study, an improvement was obtained in the pathophysiological processes of bone tissue regeneration under conditions of a bicortical defect with minimal presence of spongy substance in the damage zone.

scholarly journals Polydioxanone-Based Membranes for Bone Regeneration

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1685
Author(s):  
Sybele Saska ◽  
Livia Pilatti ◽  
Edvaldo Santos de Sousa Silva ◽  
Magda Aline Nagasawa ◽  
Diana Câmara ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Clinical Signs ◽  
Systemic Toxicity ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Crystallinity Degree ◽  
Reaction Index ◽  
Collagen Membranes

Resorbable synthetic and natural polymer-based membranes have been extensively studied for guided tissue regeneration. Alloplastic biomaterials are often used for tissue regeneration due to their lower immunoreactivity when compared with allogeneic and xenogeneic materials. Plenum® Guide is a synthetic membrane material based on polydioxanone (PDO), whose surface morphology closely mimics the extracellular matrix. In this study, Plenum® Guide was compared with collagen membranes as a barrier material for bone-tissue regeneration in terms of acute and subchronic systemic toxicity. Moreover, characterizations such as morphology, thermal analysis (Tm = 107.35 °C and crystallinity degree = 52.86 ± 2.97 %, final product), swelling (thickness: 0.25 mm ≅ 436% and 0.5 mm ≅ 425% within 24 h), and mechanical tests (E = 30.1 ± 6.25 MPa; σ = 3.92 ± 0.28 MPa; ε = 287.96 ± 34.68%, final product) were performed. The in vivo results revealed that the PDO membranes induced a slightly higher quantity of newly formed bone tissue than the control group (score: treated group = 15, control group = 13) without detectable systemic toxicity (clinical signs and evaluation of the membranes after necropsy did not result in differences between groups, i.e., non-reaction -> tissue-reaction index = 1.3), showing that these synthetic membranes have the essential characteristics for an effective tissue regeneration. Human adipose-derived stem cells (hASCs) were seeded on PDO membranes; results demonstrated efficient cell migration, adhesion, spread, and proliferation, such that there was a slightly better hASC osteogenic differentiation on PDO than on collagen membranes. Hence, Plenum® Guide membranes are a safe and efficient alternative for resorbable membranes for tissue regeneration.

scholarly journals The Morphological Characteristics of Bone Tissue Regeneration in the Application of Bone Grafting Auto-Mixture

2019 ◽  
pp. 57-62
Author(s):  
E. A. Nadyrov ◽  
V. I. Nikolaev ◽  
S. I. Kirilenko ◽  
V. V. Rozhin ◽  
N. G. Maltseva ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Granulation Tissue ◽  
Morphological Characteristics ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Laboratory Animals ◽  
Control Group ◽  
Male Wistar Rats ◽  
Experimental Group

Objective: to study the morphological and morphometric features of bone tissue regeneration in the application of the native transplantation automixture during bone defects replacement in an experiment.Material and methods. Male Wistar rats weighing 180-200 g. and aged 6-7 months were used. The bone defect was modeled using a 1.2 mm diameter cutter (experimental group). A similar defect of the middle third of the right tibia without filling the defect with bone tissue was formed in the animals of the control group. The animals were taken out of the experiment on the 3rd, 7th, 14th, and 30th days of the experiment (6 animals per each observation period). Histological sections 4-5 µm thick were stained with hematoxylin and eosin. The areas of necrosis, granulation tissue, bone trabecules (mm2) were determined.Results. The laboratory animals in which the bone mixture had been transplanted revealed the growth of the area occupied by granulation tissue from the 3rd to 7th day of the experiment, and later its area had decreased by the 30th day compared to the control group, which was associated with faster transformation of granulation tissue into bone trabecules and formation of the foci of cerebrospinal hematopoiesis. On the 7th day of the experiment, the area of necrosis in the control group significantly exceeded the indicators of the experimental group (р ˂ 0.001). On the 14th day of the experiment necrosis in the animals of the experimental group was absent, while in the control one there were still some necrosis foci (0.541 ± 0.014 mm2). Starting from the 7th of the experiment, immature bone tissue began to form within the area of the bone tissue defect. At the same time, its area in the animals of the experimental group was statistically significantly larger on the 7th, 14th and 30th days in comparison with the control group during all observation terms (р ˂ 0.001).Conclusion. The study has showed a higher rate of bone tissue formation in the bone defects in the experimental animals after autotransplantation of the bone mixture. The obtained results of bone autoplasty can be a theoretical basis for the development of the methods of the treatment of bone defects of any origin.

scholarly journals Controlled Release Strategies for the Combination of Fresh and Lyophilized Platelet-Rich Fibrin on Bone Tissue Regeneration

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Zhongshuang Liu ◽  
Han Jin ◽  
Qi Xie ◽  
Zhuling Jiang ◽  
Shouli Guo ◽  
...  
Keyword(s):  
Growth Factor ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Transforming Growth Factor ◽  
Release Kinetics ◽  
Bone Tissue Regeneration ◽  
Osteogenic Potential ◽  
Control Group ◽  
Platelet Rich Fibrin ◽  
Proliferation And Differentiation

The aim of the present study was to investigate growth factors release kinetics for the combination of fresh platelet-rich fibrin (F-PRF) and lyophilized PRF (L-PRF) with different ratios to promote bone tissue regeneration. First, we quantified the level of transforming growth factor-β1 (TGF-β1), vascular endothelial growth factor (VEGF), and platelet-derived growth factor-AB (PDGF-AB) in vitro and analyzed their release kinetics from F-PRF, L-PRF, and the fresh/lyophilized PRF in different weight ratios (F:L=1:1, 1:3, 1:5). The second experimental phase was to investigate the proliferation and differentiation of bone mesenchymal stem cells (BMSCs) as a functional response to the factors released. To further test the osteogenic potential in vivo, different scaffolds (F-PRF, or L-PRF, or F:L=1:1) were implanted in rabbit cranial bone defects. There was a statistically significant increase in proliferation and differentiation of BMSCs when the culture medium contained different PRF exudates collected at day 14 compared with the negative control group. The results showed that the new bone formation in the fresh/lyophilized PRF (1:1) was much more than that of other groups in defects at both 6 and 12 weeks. Our data suggested growth factor concentration and release kinetics as a consequence of fresh and lyophilized PRF combination, which is an effective way for promoting bone regeneration.

Thermal and in vitro evaluation of a composite material pHEMA/Chitosan/Hydroxyapatite

2015 ◽  
Vol 1767 ◽  
pp. 133-138
Author(s):  
Areli.M. Salgado-Delgado ◽  
Zully Vargas-Galarza ◽  
René Salgado-Delgado ◽  
Efraín Rubio-Rosas ◽  
Edgar García-Hernández ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Body Fluid ◽  
Bone Tissue Regeneration ◽  
Gravimetric Analysis ◽  
Hydroxyethyl Methacrylate ◽  
Thermal Gravimetric ◽  
In Vitro Bioactivity ◽  
Bioactive Materials

ABSTRACTBioactive materials based on polymer/hydroxyapatite are currently being extensively investigated as materials for promotion of bone tissue regeneration and reconstruction [1]. In this work, a material interpenetrating based on poly 2-hydroxyethyl methacrylate (pHEMA), Chitosan and hydroxyapatite (HA) was prepared following the methodology of the foaming gas Damla Çetin [2], generating an interpenetrated network with the chitosan filled with hydroxyapatite. The materials were evaluated by thermal gravimetric analysis (TGA) and in vitro bioactivity [3] (SBF) and characterized by using scanning electron microscopy (SEM). The TGA studies suggested that there was not existence of possible interactions between polymers and HA but there is a thermal stability increase in the HA content. Meanwhile, SBF and its characterization by SEM, was found that the materials are bioactives as indicated by the formation of a bone-like apatite layer after immersion in simulated body fluid, indicating the potential of this material for use in bone tissue engineering.

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