Suppression of Immune Response to Adenovirus Serotype 5 Vector by Immunization with Peptides Containing an MHC Class II Epitope and a Thio-Oxidoreductase Motif

2016 ◽  
Vol 27 (3) ◽  
pp. 230-243 ◽  
Author(s):  
Wei Miao ◽  
Roxana Roohi Ahangarani ◽  
Vincent Carlier ◽  
Luc Vander Elst ◽  
Jean-Marie Saint-Remy
Keyword(s):  
Immune Response ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Adenovirus Serotype ◽  
Serotype 5 ◽  
Suppression Of Immune Response

MHC Sınıf I ve MHC Sınıf II Gen Düzenlenmesi

2020 ◽  
Vol 8 (3) ◽  
pp. 144-156
Author(s):  
Şule KARATAŞ ◽  
Fatma SAVRAN OĞUZ
Keyword(s):  
Immune Response ◽  
T Cells ◽  
Gene Regulation ◽  
Mhc Class I ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Class I ◽  
Mhc Molecules ◽  
Class Ii Mhc ◽  
Regulation Mechanisms

Introduction: Peptides obtained by processing intracellular and extracellular antigens are presented to T cells to stimulate the immune response. This presentation is made by peptide receptors called major histocompatibility complex (MHC) molecules. The regulation mechanisms of MHC molecules, which have similar roles in the immune response, especially at the gene level, have significant differences according to their class. Objective: Class I and class II MHC molecules encoded by MHC genes on the short arm of the sixth chromosome are peptide receptors that stimulate T cell response. These peptides, which will enable the recognition of the antigen from which they originate, are loaded into MHC molecules and presented to T cells. Although the principles of loading and delivering peptides are similar for both molecules, the peptide sources and peptide loading mechanisms are different. In addition, class I molecules are expressed in all nucleated cells while class II molecules are expressed only in Antigen Presentation Cells (APC). These differences; It shows that MHC class I is not expressed by exactly the same transcriptional mechanisms as MHC class II. In our article, we aimed to compare the gene expressions of both classes and reveal their similarities and differences. Discussion and Conclusion: A better understanding of the transcriptional mechanisms of MHC molecules will reveal the role of these molecules in diseases more clearly. In our review, we discussed MHC gene regulation mechanisms with presence of existing informations, which is specific to the MHC class, for contribute to future research. Keywords: MHC class I, MHC class II, MHC gene regulation, promoter, SXY module, transcription

scholarly journals The impact of DM on MHC class II–restricted antigen presentation can be altered by manipulation of MHC–peptide kinetic stability

2006 ◽  
Vol 203 (5) ◽  
pp. 1319-1328 ◽  
Author(s):  
Christopher A. Lazarski ◽  
Francisco A. Chaves ◽  
Andrea J. Sant
Keyword(s):  
Immune Response ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Kinetic Stability ◽  
Histocompatibility Complex ◽  
Peptide Interactions ◽  
Peptide Complexes ◽  
Antigen Presenting ◽  
Intrinsic Kinetic ◽  
The Impact

DM edits the peptide repertoire presented by major histocompatibility complex class II molecules by professional antigen-presenting cells (APCs), favoring presentation of some peptides over others. Despite considerable research by many laboratories, there is still significant uncertainty regarding the biochemical attributes of class II–peptide complexes that govern their susceptibility to DM editing. Here, using APCs that either do or do not express DM and a set of unrelated antigens, we found that the intrinsic kinetic stability of class II–peptide complexes is tightly correlated with the effects of DM editing within APCs. Furthermore, through the use of kinetic stability variants of three independent peptides, we demonstrate that increasing or decreasing the kinetic stability of class II–peptide complexes causes a corresponding alteration in DM editing. Finally, we show that the spontaneous kinetic stability of class II complexes correlates directly with the efficiency of presentation by DM+ APCs and the immunodominance of that class II–peptide complex during an immune response. Collectively, these results suggest that the pattern of DM editing in APCs can be intentionally changed by modifying class II–peptide interactions, leading to the desired hierarchy of presentation on APCs, thereby promoting recruitment of CD4 T cells specific for the preferred peptides during an immune response.

Diversity of MHC class II DAB1 in the koala (Phascolarctos cinereus)

2012 ◽  
Vol 60 (1) ◽  
pp. 1 ◽  
Author(s):  
Sarah E. Jobbins ◽  
Claire E. Sanderson ◽  
Joanna E. Griffith ◽  
Mark B. Krockenberger ◽  
Katherine Belov ◽  
...  
Keyword(s):  
Immune Response ◽  
Mhc Class Ii ◽  
Genetic Research ◽  
Class Ii ◽  
Population Diversity ◽  
Environment Interaction ◽  
Phascolarctos Cinereus ◽  
Mhc Molecules ◽  
Adaptive Immune ◽  
Class Ii Mhc

The host immune response is an important factor determining the outcome of the host–pathogen–environment interaction. At the gateway between the innate and adaptive immune systems are MHC molecules, which facilitate antigen presentation to T lymphocytes, and initiate the adaptive immune response. Despite their integral role in adaptive immunity, the genes encoding class II MHC molecules have not been examined directly in koalas. Furthermore, indirect historical evidence suggests that this species might lack functional diversity in class II MHC genes, with potential implications for disease susceptibility. We have examined diversity in the β chain genes of the koala class II MHC DA gene family and identified 23 alleles, including several atypical alleles. The levels of diversity observed are consistent with other marsupial and eutherian species, and do not support the paucity of variation suggested by the early literature. These findings are relevant to the conservation management of koalas and provide both a benchmark for maintaining population diversity and a platform for further conservation genetic research in this species.

scholarly journals Cutting Edge: Permissive MHC Class II Allele Changes the Pattern of Antitumor Immune Response Resulting in Failure of Tumor Rejection

2009 ◽  
Vol 182 (3) ◽  
pp. 1242-1246 ◽  
Author(s):  
Elena N. Klyushnenkova ◽  
Diana V. Kouiavskaia ◽  
Carla A. Berard ◽  
Richard B. Alexander
Keyword(s):  
Immune Response ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Cutting Edge ◽  
Tumor Rejection ◽  
Antitumor Immune Response

scholarly journals Genetic Dissection of Primary and Secondary Responses to a Widespread Natural Pathogen of the Gut,Eimeria vermiformis

2000 ◽  
Vol 68 (11) ◽  
pp. 6273-6280 ◽  
Author(s):  
Adrian L. Smith ◽  
Adrian C. Hayday
Keyword(s):  
Immune Response ◽  
T Cells ◽  
T Cell ◽  
Mhc Class Ii ◽  
Class Ii ◽  
Primary Response ◽  
Class I ◽  
Class Ii Mhc ◽  
Ifn Γ ◽  
Αβ T Cells

ABSTRACT Because most pathogens initially challenge the body at epithelial surfaces, it is important to dissect the mechanisms that underlie T-cell responses to infected epithelial cells in vivo. The coccidian parasites of the genus Eimeria are protozoan gut pathogens that elicit a potent, protective immune response in a wide range of host species. CD4+ αβ T cells and gamma interferon (IFN-γ) are centrally implicated in the primary immunoprotective response. To define any additional requirements for the primary response and to develop a comparison between the primary and the secondary response, we have studied Eimeria infections of a broad range of genetically altered mice. We find that a full-strength primary response depends on β2-microglobulin (class I major histocompatibility complex [MHC] and class II MHC and on IFN-γ and interleukin-6 (IL-6) but not on TAP1, perforin, IL-4, Fas ligand, or inducible nitric oxide synthetase. Indeed, MHC class II-deficient and IFN-γ-deficient mice are as susceptible to primary infection as mice deficient in all αβ T cells. Strikingly, the requirements for a highly effective αβ-T-cell-driven memory response are less stringent, requiring neither IFN-γ nor IL-6 nor class I MHC. The class II MHC dependence was also reduced, with adoptively transferable immunity developing in MHC class II−/− mice. Besides the improved depiction of an immune response to a natural gut pathogen, the finding that effective memory can be elicited in the absence of primary effector responses appears to create latitude in the design of vaccine strategies.

Benfotiamine reduces dendritic cell inflammatory potency

2020 ◽  
Vol 20 ◽  
Author(s):  
Neda Djedovic ◽  
Iva Božić ◽  
Đorđe Miljković ◽  
Irena Lavrnja
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Immune Response ◽  
Dendritic Cells ◽  
Mhc Class Ii ◽  
Morphological Changes ◽  
Marrow Cell ◽  
Class Ii ◽  
Immunofluorescent Staining ◽  
Mhc Class Ii Molecules

Background: Benfotiamine is a synthetic liposoluble derivative of vitamin B1 that has been shown to have antiinflammatory properties. Objective: To study the effects of benfotiamine on dendritic cells. Methods: Dendritic cells were obtained from murine bone marrow precursor cells in the presence of GM-CSF. Benfotiamine was applied to the cell culture during the process of bone marrow cell differentiation into dendritic cells. Dendritic cells were stimulated with lipopolysaccharide (LPS) and expression of MHC class II molecules and CD86 was determined by flow cytometry, while levels of tumor necrosis factor (TNF) and interleukin (IL)-1β in cell culture supernatants were measured by ELISA. F-Actin, NF-κB and Nrf2 were visualized by immunofluorescent staining and microscopy. Results: Benfotiamine potently reduced LPS-induced expression of MHC class II molecules and CD86, in addition to suppressing the release of pro-inflammatory cytokines TNF and IL-1β. It also prevented LPS-imposed morphological changes of dendritic cells, i.e. enlargement and intensified protrusions. The effects were paralleled with the reduction of NF-κB translocation to the nucleus, but not of Nrf2 activation inhibition. Conclusion: Having in mind the importance of dendritic cells for the configuration of the immune response, our results imply that benfotiamine has the ability to regulate the immune response through inhibition of inflammatory properties of dendritic cells.

MHC class II antigen-associated invariant chain on renal cell cancer may contribute to the anti-tumor immune response of the host

1996 ◽  
Vol 109 (1-2) ◽  
pp. 15-21 ◽  
Author(s):  
Toshihiro Saito ◽  
Motohiko Kimura ◽  
Takashi Kawasaki ◽  
Shotaro Sato ◽  
Yoshihiko Tomita
Keyword(s):  
Immune Response ◽  
Mhc Class Ii ◽  
Renal Cell Cancer ◽  
Renal Cell ◽  
Cell Cancer ◽  
Class Ii ◽  
Invariant Chain ◽  
Class Ii Antigen ◽  
Mhc Class Ii Antigen
Sign in / Sign up

Export Citation Format

Share Document