A Polysaccharide Isolated from Ecklonia cava Fermented by Lactobacillus brevis Inhibits the Inflammatory Response by Suppressing the Activation of Nuclear Factor-κB in Lipopolysaccharide-Induced RAW 264.7 Macrophages

2011 ◽  
Vol 14 (12) ◽  
pp. 1546-1553 ◽  
Author(s):  
Won-Woo Lee ◽  
Ginnae Ahn ◽  
Janaka Priyalal Wijesinghe Arachchillage ◽  
Young Mog Kim ◽  
Se-Kwon Kim ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Lactobacillus Brevis ◽  
Nuclear Factor Κb ◽  
Ecklonia Cava ◽  
Raw 264.7 ◽  
Nuclear Factor ◽  
Raw 264.7 Macrophages

Nuclear factor-κB mediates over-expression of cyclooxygenase-2 during activation of RAW 264.7 macrophages in selenium deficiency

2002 ◽  
Vol 32 (9) ◽  
pp. 890-897 ◽  
Author(s):  
Faith Zamamiri-Davis ◽  
Ying Lu ◽  
Jerry T Thompson ◽  
K.Sandeep Prabhu ◽  
Padala V Reddy ◽  
...  
Keyword(s):  
Selenium Deficiency ◽  
Nuclear Factor Κb ◽  
Cyclooxygenase 2 ◽  
Raw 264.7 ◽  
Nuclear Factor ◽  
Raw 264.7 Macrophages ◽  
Over Expression

scholarly journals Lutonarin from Barley Seedlings Inhibits the Lipopolysacchride-Stimulated Inflammatory Response of RAW 264.7 Macrophages by Suppressing Nuclear Factor-κB Signaling

Molecules ◽  
2021 ◽  
Vol 26 (6) ◽  
pp. 1571
Author(s):  
Ji Yeong Yang ◽  
So-Yeun Woo ◽  
Mi Ja Lee ◽  
Hyun Young Kim ◽  
Jin Hwan Lee ◽  
...  
Keyword(s):  
Nuclear Translocation ◽  
Nuclear Factor Κb ◽  
Ultra Performance Liquid Chromatography ◽  
Raw 264.7 ◽  
Nuclear Factor ◽  
Raw 264.7 Macrophages ◽  
Tnf Α ◽  
Anti Inflammatory ◽  
Oxide Synthase ◽  
Necrosis Factor

Extracts from barley seedlings (BS) have known antioxidant and anti-inflammatory activities. The flavonoid lutonarin (LN) is a component of BS extract and has several known bioactivities. Here, we evaluated LN anti-inflammatory efficacy against lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Lutonarin was isolated from BS by methanol extraction and characterized by ultra-performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF-MS/MS). Lutonarin did not reduce the viability or enhance the apoptosis rate of RAW 264.7 macrophages at concentrations up to 150 µM. Concentrations within 20–60 µM dose-dependently suppressed the LPS-induced expression, phosphorylation, and nuclear translocation of the inflammatory transcription factor nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). Furthermore, LN suppressed the LPS-induced upregulation of proinflammatory cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-α and of the inflammatory enzyme cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS). Lutonarin may be a safe and effective therapeutic agent for alleviation of pathological inflammation.

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