Design and Fabrication of Sub-mm-Sized Modules Containing Encapsulated Cells for Modular Tissue Engineering

2007 ◽  
Vol 13 (5) ◽  
pp. 1069-1078 ◽  
Author(s):  
Alison P. McGuigan ◽  
Michael V. Sefton
Keyword(s):  
Tissue Engineering ◽  
Encapsulated Cells ◽  
Modular Tissue Engineering

scholarly journals Three-Dimensional Printable Hydrogel Using a Hyaluronic Acid/Sodium Alginate Bio-Ink

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 794 ◽  
Author(s):  
Su Jeong Lee ◽  
Ji Min Seok ◽  
Jun Hee Lee ◽  
Jaejong Lee ◽  
Wan Doo Kim ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Hyaluronic Acid ◽  
Sodium Alginate ◽  
Three Dimensional ◽  
Fibroblast Cell ◽  
Chemical Crosslinking ◽  
Fibroblast Cell Line ◽  
Engineering Applications ◽  
Encapsulated Cells ◽  
Crosslinking Agents

Bio-ink properties have been extensively studied for use in the three-dimensional (3D) bio-printing process for tissue engineering applications. In this study, we developed a method to synthesize bio-ink using hyaluronic acid (HA) and sodium alginate (SA) without employing the chemical crosslinking agents of HA to 30% (w/v). Furthermore, we evaluated the properties of the obtained bio-inks to gauge their suitability in bio-printing, primarily focusing on their viscosity, printability, and shrinkage properties. Furthermore, the bio-ink encapsulating the cells (NIH3T3 fibroblast cell line) was characterized using a live/dead assay and WST-1 to assess the biocompatibility. It was inferred from the results that the blended hydrogel was successfully printed for all groups with viscosities of 883 Pa∙s (HA, 0% w/v), 1211 Pa∙s (HA, 10% w/v), and 1525 Pa∙s, (HA, 30% w/v) at a 0.1 s−1 shear rate. Their structures exhibited no significant shrinkage after CaCl2 crosslinking and maintained their integrity during the culture periods. The relative proliferation rate of the encapsulated cells in the HA/SA blended bio-ink was 70% higher than the SA-only bio-ink after the fourth day. These results suggest that the 3D printable HA/SA hydrogel could be used as the bio-ink for tissue engineering applications.

scholarly journals Effects of Encapsulated Cells on the Physical–Mechanical Properties and Microstructure of Gelatin Methacrylate Hydrogels

2019 ◽  
Vol 20 (20) ◽  
pp. 5061 ◽  
Author(s):  
Srikumar Krishnamoorthy ◽  
Behnam Noorani ◽  
Changxue Xu
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Cell Viability ◽  
Physical Properties ◽  
Pore Size ◽  
Cell Density ◽  
Tensile Modulus ◽  
Encapsulated Cells ◽  
3T3 Fibroblasts ◽  
Cell Densities

Gelatin methacrylate (GelMA) has been gaining popularity in recent years as a photo-crosslinkable biomaterial widely used in a variety of bioprinting and tissue engineering applications. Several studies have established the effects of process-based and material-based parameters on the physical–mechanical properties and microstructure of GelMA hydrogels. However, the effect of encapsulated cells on the physical–mechanical properties and microstructure of GelMA hydrogels has not been fully understood. In this study, 3T3 fibroblasts were encapsulated at different cell densities within the GelMA hydrogels and incubated over 96 h. The effects of encapsulated cells were investigated in terms of mechanical properties (tensile modulus and strength), physical properties (swelling and degradation), and microstructure (pore size). Cell viability was also evaluated to confirm that most cells were alive during the incubation. It was found that with an increase in cell density, the mechanical properties decreased, while the degradation and the pore size increased.

scholarly journals Liquefied Capsules: Geometrically Controlled Liquefied Capsules for Modular Tissue Engineering Strategies (Adv. Biosys. 11/2020)

2020 ◽  
Vol 4 (11) ◽  
pp. 2070114
Author(s):  
Sara Nadine ◽  
Sónia G. Patrício ◽  
Cristina C. Barrias ◽  
Insung S. Choi ◽  
Michiya Matsusaki ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Modular Tissue Engineering

scholarly journals Fabrication of a Microfluidic System Using Micromolded Alginate Gel as a Sacrificial Material for Tissues Engineering

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Meng Wang ◽  
Xiaoning Yang ◽  
Lanju Liang
Keyword(s):  
Tissue Engineering ◽  
Ion Exchange ◽  
Metabolic Activity ◽  
Calcium Alginate ◽  
Microfluidic System ◽  
Sacrificial Template ◽  
Encapsulated Cells ◽  
Alginate Gel ◽  
Sacrificial Material ◽  
Vascular Channels

We described a sacrificial molding for the formation of microfluidic networks. In this molding, the micromolded calcium alginate (Ca-Alg) is introduced as a sacrificial template. The basis of this procedure is fabricating a micromolded Ca-Alg hydrogel and encapsulating this model within a second gel and removing it by ion-exchange to leave a microchannel in the remaining gel. This microfluidic system can readily deliver solutes into the channels and even control the transport of solutes from channels into the bulk of the gels. Furthermore, the perfused vascular channels can sustain the metabolic activity of encapsulated cells, indicating the feasibility of this microfluidic system in the field of tissue engineering.

Geometrically Controlled Liquefied Capsules for Modular Tissue Engineering Strategies

2020 ◽  
Vol 4 (11) ◽  
pp. 2000127
Author(s):  
Sara Nadine ◽  
Sónia G. Patrício ◽  
Cristina C. Barrias ◽  
Insung S. Choi ◽  
Michiya Matsusaki ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Modular Tissue Engineering

scholarly journals Controlled Tubular Unit Formation from Collagen Film for Modular Tissue Engineering

2016 ◽  
Vol 3 (11) ◽  
pp. 2860-2868 ◽  
Author(s):  
Jianming Sang ◽  
Xiang Li ◽  
Yue Shao ◽  
Zida Li ◽  
Jianping Fu
Keyword(s):  
Tissue Engineering ◽  
Collagen Film ◽  
Unit Formation ◽  
Modular Tissue Engineering
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