scholarly journals β-catenin-mediated Wnt signal transduction proceeds through an endocytosis-independent mechanism

2020 ◽  
Vol 31 (13) ◽  
pp. 1425-1436 ◽  
Author(s):  
Ellen Youngsoo Rim ◽  
Leigh Katherine Kinney ◽  
Roeland Nusse
Keyword(s):  
Signal Transduction ◽  
Real Time ◽  
Cell Lines ◽  
Wnt Pathway ◽  
Receptor Endocytosis ◽  
Pathway Activation ◽  
Independent Mechanism ◽  
Multiple Cell ◽  
Wnt Signal ◽  
Time Assessment

A novel tool for quantitative, real-time assessment of Wnt pathway activation was combined with genetic disruption of endocytosis to determine whether receptor endocytosis is required for Wnt signal transduction. Our results in multiple cell lines support that clathrin- or caveolin-mediated endocytosis is dispensable for Wnt signal transduction.

scholarly journals β-catenin-Mediated Wnt Signal Transduction Proceeds Through an Endocytosis-Independent Mechanism

2020 ◽  
Author(s):  
Ellen Youngsoo Rim ◽  
Leigh Katherine Kinney ◽  
Roel Nusse
Keyword(s):  
Signal Transduction ◽  
Gene Transcription ◽  
Wnt Pathway ◽  
Target Gene ◽  
Single Cells ◽  
Receptor Endocytosis ◽  
Pathway Activation ◽  
Essential Components ◽  
Wnt Signal ◽  
Target Gene Transcription

The Wnt pathway is a key intercellular signaling cascade that regulates development, tissue homeostasis, and regeneration. However, gaps remain in our understanding of the molecular events that take place between ligand-receptor binding and target gene transcription. Here we used a novel tool for quantitative, real-time assessment of endogenous pathway activation, measured in single cells, to answer an unresolved question in the field – whether receptor endocytosis is required for Wnt signal transduction. We combined knockdown or knockout of essential components of Clathrin-mediated endocytosis with quantitative assessment of Wnt signal transduction in mouse embryonic stem cells (mESCs). Disruption of Clathrin-mediated endocytosis did not affect accumulation and nuclear translocation of β-catenin, as measured by single-cell live imaging of endogenous β-catenin, and subsequent target gene transcription. Disruption of another receptor endocytosis pathway, Caveolin-mediated endocytosis, did not affect Wnt pathway activation either. These results, confirmed in multiple cell lines, suggest that endocytosis is not a general requirement for Wnt signal transduction. We show that off-target effects of a drug used to inhibit endocytosis may be one source of the discrepancy among reports on the role of endocytosis in Wnt signaling.

Interactions and dynamics of hedgehog and Wnt pathway activation levels in ovarian cancer cell lines.

2010 ◽  
Vol 28 (15_suppl) ◽  
pp. e15515-e15515 ◽  
Author(s):  
S. Schmid ◽  
F. Zhang ◽  
M. Zhang ◽  
B. He ◽  
D. Jablons ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Wnt Pathway ◽  
Ovarian Cancer Cell ◽  
Cancer Cell Lines ◽  
Ovarian Cancer Cell Lines ◽  
Pathway Activation

scholarly journals WIF-1 gene inhibition and Wnt signal transduction pathway activation in NSCLC tumorigenesis

2017 ◽  
Vol 13 (3) ◽  
pp. 1183-1188 ◽  
Author(s):  
Qiong Tang ◽  
Hui Zhao ◽  
Bingjun Yang ◽  
Li Li ◽  
Qiulan Shi ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Signal Transduction Pathway ◽  
Transduction Pathway ◽  
Pathway Activation ◽  
Gene Inhibition ◽  
Wnt Signal
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