Effect of Ascorbic Acid on Osmotic Fragility of Normal Erythrocytes in Saline

1950 ◽  
Vol 20 (7) ◽  
pp. 641-643 ◽  
Author(s):  
V. Traina
Keyword(s):  
Ascorbic Acid ◽  
Osmotic Fragility

scholarly journals Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid

2012 ◽  
Vol 79 (1) ◽  
Author(s):  
Folashade Olaifa ◽  
Joseph O. Ayo ◽  
Suleiman F. Ambali ◽  
Peter I. Rekwot
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Osmotic Fragility ◽  
Blood Samples ◽  
Experimental Animals ◽  
Erythrocyte Osmotic Fragility ◽  
Nacl Concentration ◽  
Experimental Subjects ◽  
Apparently Healthy

Experiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as experimental subjects. Six donkeys administered orally with AA (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not administered with AA served as controls. Blood samples were collected pre- and post-packing from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl) concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the value was significantly (P < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the experimental donkeys. The post-packing MDA concentration obtained in the control donkeys was 39.62 µmol ± 4.16 µmol, and was not significantly different (P > 0.05) from the value of 35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which was ameliorated by AA administration.

Effect of ascorbic acid administration on erythrocyte osmotic fragility of pigs transported by road during the hot-dry season

2011 ◽  
Vol 35 (4) ◽  
pp. 245-254 ◽  
Author(s):  
Olayinka O. Asala ◽  
Joseph O. Ayo ◽  
Peter I. Rekwot ◽  
Ndazo S. Minka ◽  
David O. Omoniwa ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Osmotic Fragility ◽  
Dry Season ◽  
Erythrocyte Osmotic Fragility ◽  
Hot Dry Season ◽  
Acid Administration

scholarly journals Antibiotic-Induced Alterations in the Osmotic Resistance of Erythrocytes is Modulated by β-Carotene and L –Ascorbic Acid

2006 ◽  
Vol 1 (6) ◽  
pp. 1934578X0600100
Author(s):  
Suaib Luqman ◽  
K V Obli Prabu ◽  
Anirban Pal ◽  
Dharmendra Saikia ◽  
Mahendra P Darokar ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Mammalian Cells ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Osmotic Fragility ◽  
Protective Role ◽  
Vitamin Supplementation ◽  
Hypotonic Stress ◽  
Osmotic Resistance Of Erythrocytes ◽  
Β Carotene

Ciprofloxacin, Erythromycin and Norfloxacin are potent anti-microbials. These antibiotics, besides affecting bacteria, also exert toxic effects on mammalian cells. The present work was undertaken to study the effect of these antibiotics on the osmotic fragility profile of erythrocytes and the protective role played by vitamin supplementation, which exerts a stabilizing effect on the erythrocytes making them more resistant to lysis under hypotonic stress. The protective effect of β-carotene and L-ascorbic acid was significant (p < 0.01) at 0.1 mM and 0.01 mM concentrations. No significant effect was observed at lower concentrations. The free radical scavenging activity and effect of these vitamins on membrane fluidity and permeability may explain the protective role against the damage induced by the antibiotics on the erythrocytes membrane.

scholarly journals Protective role of tocopherol and ascorbic acid in taxol-treated human erythrocytes in vitro

2017 ◽  
Vol 1 ◽  
pp. 239784731770581
Author(s):  
Uzma Faridi ◽  
Fatema Alatawi ◽  
Magda Mostafa
Keyword(s):  
Ascorbic Acid ◽  
Superoxide Dismutase ◽  
Side Effects ◽  
Protective Effect ◽  
Haematological Toxicity ◽  
Osmotic Fragility ◽  
Natural Antioxidants ◽  
Drug Induced ◽  
Osmotic Lysis

Taxol is a well-known anticancer agent. It is used for the treatment of several kinds of cancer, including breast cancer, lung cancer and ovarian cancer. In spite of being a good chemotherapeutic agent, taxol has several side effects. Drug-induced haemolytic anaemia is one of the most common side effects of taxol. This study investigated the haemolytic effect of taxol on normal erythrocytes and the protective effect of natural antioxidants ascorbic acid and tocopherol in the presence of taxol. We evaluated the osmotic fragility and the activity of enzymes superoxide dismutase and catalase of erythrocytes in the presence of taxol alone and taxol in combination with tocopherol and ascorbic acid. Taxol-induced haematological perturbation significantly caused haemolysis and reduced the activities of superoxide dismutase and catalase in erythrocytes. The antioxidants tocopherol and ascorbic acid demonstrated a protective effect when added to taxol. The combination of tocopherol with taxol significantly protected the osmotic lysis of erythrocytes and increased the activities of superoxide dismutase but had less effect on catalase. Ascorbic acid showed significant protection of erythrocytes from osmotic lysis but didn’t show any significant effect on superoxide and catalase. The results suggest that both antioxidants, especially tocopherol, could exhibit a protective effect against taxol-induced haematological toxicity.

Comparative Study of Ascorbic Acid Levels in Gastric Secretion, Blood, Urine and Saliva

1951 ◽  
Vol 18 (2) ◽  
pp. 224-229 ◽  
Author(s):  
Joseph T. Freeman ◽  
Roberta Hafkesbring
Keyword(s):  
Ascorbic Acid ◽  
Gastric Secretion ◽  
Comparative Study
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