scholarly journals Effect of packing on changes in erythrocyte osmotic fragility and malondialdehyde concentration in donkeys administered with ascorbic acid

2012 ◽  
Vol 79 (1) ◽  
Author(s):  
Folashade Olaifa ◽  
Joseph O. Ayo ◽  
Suleiman F. Ambali ◽  
Peter I. Rekwot
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Osmotic Fragility ◽  
Blood Samples ◽  
Experimental Animals ◽  
Erythrocyte Osmotic Fragility ◽  
Nacl Concentration ◽  
Experimental Subjects ◽  
Apparently Healthy

Experiments were performed with the aim of investigating the effect of packing on erythrocyte osmotic fragility (EOF) and malondialdehyde (MDA) concentration in donkeys, and the effect of ascorbic acid (AA). Twelve apparently healthy donkeys raised under the traditional extensive system served as experimental subjects. Six donkeys administered orally with AA (200 mg/kg) and subjected to packing were used as experimental animals, whilst six others not administered with AA served as controls. Blood samples were collected pre- and post-packing from all the donkeys for the determination of MDA and EOF. At 0.3% Sodium Chloride (NaCl) concentration, the percentage haemolysis was 93.69% ± 2.21% in the control donkeys and the value was significantly (P < 0.05) higher than the value of 71.31% ± 8.33%, recorded in the experimental donkeys. The post-packing MDA concentration obtained in the control donkeys was 39.62 µmol ± 4.16 µmol, and was not significantly different (P > 0.05) from the value of 35.97 µmol ± 2.88 µmol recorded in the experimental donkeys. In conclusion, the increase in haemolysis obtained in the donkeys suggested that packing induced oxidative stress, which was ameliorated by AA administration.

scholarly journals Antioxidant capacity of lipid- and water-soluble antioxidants in dogs with subclinical myxomatous mitral valve degeneration anaesthetised with propofol or sevoflurane

2020 ◽  
Author(s):  
Katerina Tomsič ◽  
Alenka Nemec Svete ◽  
Ana Nemec ◽  
Aleksandra Domanjko Petrič ◽  
Tatjana Pirman ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mitral Valve ◽  
Antioxidant Capacity ◽  
Early Stage ◽  
Water Soluble ◽  
Oxygen Species ◽  
Dental Procedure ◽  
Blood Samples ◽  
Sampling Times

Abstract Background: Antioxidants located in both the hydrophilic and lipophilic compartments of plasma act as a defence system against reactive oxygen species (ROS). . Excessive production of ROS during anaesthesia affects the antioxidant capacity of plasma and may result in oxidative stress. The aim of this study was to evaluate the antioxidant capacity of lipid- (ACL) and water-soluble (ACW) antioxidants in client-owned dogs diagnosed with periodontal disease and early-stage myxomatous mitral valve degeneration (MMVD) and anaesthetised for a dental procedure with propofol and sevoflurane or with propofol only. Results: Dogs with MMVD were anaesthetised with propofol and sevoflurane (MMVD/PS, n = 8) or with propofol only (MMVD/P, n = 10). Dogs with no evidence of MMVD (PS, n = 12) were anaesthetised with propofol and sevoflurane. Blood samples for determination of ACL and ACW were collected before and 5 minutes, 60 minutes and 6 hours after induction to anaesthesia. In dogs with MMVD and anaesthetised with propofol and sevoflurane, ACL was significantly higher at all sampling times when compared to control dogs. Compared to basal values, only anaesthesia maintained with propofol significantly increased ACL at 60 minutes in dogs with MMVD. In dogs with MMVD and anaesthetised with propofol, ACW increased after induction to anaesthesia and remained elevated up to 6 hours after anaesthesia. Compared to basal values, anaesthesia maintained with sevoflurane significantly increased ACW only at 60 minutes in both dogs with and without MMVD.The only difference between propofol and propofol/sevoflurane anaesthesia in dogs with MMVD was significantly higher ACW at 60 minutes after induction to anaesthesia in the propofol group. Conclusions: Regarding antioxidant capacity, propofol could be a better choice than sevoflurane for anaesthesia of dogs with early-stage MMVD, although further studies are necessary to clarify the advantage of this antioxidant capacity.

Lactate analyzer with continuous blood sampling for monitoring blood lactate during physical exercise

1991 ◽  
Vol 37 (11) ◽  
pp. 1978-1980 ◽  
Author(s):  
N Shimojo ◽  
K Fujino ◽  
S Kitahashi ◽  
M Nakao ◽  
K Naka ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Physical Exercise ◽  
Blood Lactate ◽  
Healthy Subjects ◽  
Lactate Concentration ◽  
Blood Sampling ◽  
Enzymatic Method ◽  
Blood Samples ◽  
Before And After ◽  
Apparently Healthy

Abstract To monitor changes in the concentration of blood lactate during physical exercise, we used an automated lactate analyzer based on an electro-enzymatic method with continuous blood sampling through a catheter. The lactate concentration was measured every 2 min; between measurements, the instrument was calibrated with a lactate standard. Ascorbic acid, bilirubin, hemoglobin, creatinine, uric acid, and glucose did not interfere with the measurements. The lactate concentrations in blood samples from apparently healthy subjects before and after exercise correlated well (r = 0.993) with results by the conventional enzymatic method. We measured the blood lactate concentrations in nine apparently healthy volunteers during exercise on a treadmill with an increasing workload. The point at which lactate concentrations started to increase was detected easily. Thus, the lactate analyzer is suitable for monitoring changes in blood lactate concentrations during exercise.

Erythrocyte Osmotic Fragility and Oxidative Stress in Experimental Hypothyroidism

Endocrine ◽  
2004 ◽  
Vol 25 (1) ◽  
pp. 01-06 ◽  
Author(s):  
Nuran Dariyerli ◽  
Selmin Toplan ◽  
Mehmet Can Akyolcu ◽  
Husrev Hatemi ◽  
Gunnur Yigit
Keyword(s):  
Oxidative Stress ◽  
Osmotic Fragility ◽  
Erythrocyte Osmotic Fragility ◽  
And Oxidative Stress

Effect of ascorbic acid administration on erythrocyte osmotic fragility of pigs transported by road during the hot-dry season

2011 ◽  
Vol 35 (4) ◽  
pp. 245-254 ◽  
Author(s):  
Olayinka O. Asala ◽  
Joseph O. Ayo ◽  
Peter I. Rekwot ◽  
Ndazo S. Minka ◽  
David O. Omoniwa ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Osmotic Fragility ◽  
Dry Season ◽  
Erythrocyte Osmotic Fragility ◽  
Hot Dry Season ◽  
Acid Administration

scholarly journals Comparative study for some antioxidants in correction of oxidative stress markers for experimental induced diabetic rabbits

2012 ◽  
Vol 11 (2) ◽  
pp. 25
Author(s):  
A. J. Abd
Keyword(s):  
Oxidative Stress ◽  
Ascorbic Acid ◽  
Body Weight Gain ◽  
Stress Markers ◽  
Diabetic Rabbit ◽  
New Zealand White Rabbits ◽  
Diabetic Rabbits ◽  
Histopathological Lesion

Determination of reliable biochemical parameters of experimental diabetic rabbit by alloxane monohydrates and role of diatery antioxidants (ascorbic acid, vit. A, and α-tocopherole) supplementation were investigated. Body weight gain, blood (plasma) chemistries, antioxidant enzymes and histopathological lesion were determined over 10 week in new Zealand white rabbits: control T1 group, diabetic T2 group, diabetic and ascorbic acid T3 group, diabetic and Vit. A T4 group, diabetic and α-tocopherole T5 animals group . each of dietary ascorbic acid, vit. A and α-tocopherole that given to animals of T3, T4 and T5 respectively were significantly (P≤0.01) reduced glucose level in blood than T2 diabetic group, also these antioxidants improve levels of cholesterol and triglyceride. Enzymatic activity of liver glutathione peroxidase and superoxide dismutase were significantly increasd more than of diabetic animals T2. histopathological structures of liver and pancrease confirm these results which indicate mild congestion and less degenerative signs in hepatocytes with mild degeneration of langerhans islets, generally oxidative stress resulted from diabetes and may diminished by administration of antioxidant ascorbic acid, Vit. A and α-tocopherole supplementation, and α-tocopherole group was the best group

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