Optimal Use Ratio of the Stromal Vascular Fraction (SVF): An Animal Experiment Based on Micro-CT Dynamic Detection After Large-Volume Fat Grafting

2018 ◽  
Vol 39 (6) ◽  
pp. NP213-NP224 ◽  
Author(s):  
Fang-Wei Li ◽  
Hai-Bin Wang ◽  
Jin-Ping Fang ◽  
Li Zeng ◽  
Chun-Lin Chen ◽  
...  
Keyword(s):  
Large Volume ◽  
Animal Experiment ◽  
Stromal Vascular Fraction ◽  
Fat Grafting ◽  
Micro Ct ◽  
Dynamic Detection

Lipofilling to the Buttocks and Breasts in Local Anesthesia Using VASER and Expansion Vibration Lipofilling in a Closed-Loop System

2020 ◽  
pp. 074880682095649
Author(s):  
Roland Boeni ◽  
Paul von Waechter-Gniadek
Keyword(s):  
Local Anesthesia ◽  
Large Volume ◽  
Closed Loop ◽  
Operation Time ◽  
Cost Effective ◽  
Fat Grafting ◽  
Loop System ◽  
Closed Loop System ◽  
Satisfaction Rate ◽  
Fat Extraction

Large-volume fat transfer to the buttocks and breast has rapidly become popular. Lipofilling using syringes is tedious, time-consuming, and carries the risk of contamination. Most often, systemic anesthesia is being used. This study aims to evaluate the efficacy of large-volume lipofilling in local anesthesia. Local anesthesia was performed with 2 anesthetics: lidocaine and prilocaine. We performed vibration amplification of sound energy at resonance (VASER) and reciprocating power-assisted liposuction (PAL) for fat collection in a closed-loop system using a peristaltic pump. In a reverse pump setting, fat was then used for expansion vibration lipofilling. Pre- and postoperative data were collected. There were no major complications. Liposuction volumes up to 3.100 mL were extracted, and injection volumes ranged from 200 to 1600 mL. Mean operation time was 96 minutes. Satisfaction rate was high. Anesthesia was sufficient in all patients. Using a closed-loop system for fat extraction–purification and transfer is not only time- and cost-effective but also ideal for large-volume fat grafting. Recovery time is 1 to 2 days and satisfaction rate is high.

scholarly journals Application of enhanced stromal vascular fraction and fat grafting mixed with PRP in post-traumatic lower extremity ulcers

2011 ◽  
Vol 6 (2) ◽  
pp. 103-111 ◽  
Author(s):  
Valerio Cervelli ◽  
Pietro Gentile ◽  
Barbara De Angelis ◽  
Claudio Calabrese ◽  
Alessandro Di Stefani ◽  
...  
Keyword(s):  
Lower Extremity ◽  
Stromal Vascular Fraction ◽  
Fat Grafting ◽  
Post Traumatic

scholarly journals Characterized the Adipogenic Capacity of Adipose-Derived Stem Cell, Extracellular Matrix, and Microenvironment With Fat Components Grafting

2021 ◽  
Vol 9 ◽  
Author(s):  
Wenqing Jiang ◽  
Junrong Cai ◽  
Jingyan Guan ◽  
Yunjun Liao ◽  
Feng Lu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Endothelial Cells ◽  
Fluorescent Protein ◽  
Macrophage Polarization ◽  
Lethal Dose ◽  
Stromal Vascular Fraction ◽  
Fat Grafting ◽  
Tissue Integrity ◽  
Green Fluorescent

Background: Autologous fat grafting has been a widely used technique; however, the role of adipose-derived stem cells (ASCs), extracellular matrix (ECM), and microenvironment in fat regeneration are not fully understood.Methods: Lipoaspirates were obtained and processed by inter-syringe shifting to remove adipocytes, yielding an adipocyte-free fat (Aff). Aff was then exposed to lethal dose of radiation to obtain decellularized fat (Df). To further remove microenvironment, Df was rinsed with phosphate-buffered saline (PBS) yielding rinsed decellularized fat (Rdf). Green fluorescent protein (GFP) lentivirus (LV-GFP)-transfected ASCs were added to Df to generate cell-recombinant decellularized fat (Crdf). Grafts were transplanted subcutaneously into nude mice and harvested over 3 months.Results: Removal of adipocytes (Aff) didn’t compromise the retention of fat grafts, while additional removal of stromal vascular fraction (SVF) cells (Df) and microenvironment (Rdf) resulted in poor retention by day 90 (Aff, 82 ± 7.1% vs. Df, 28 ± 6.3%; p < 0.05; vs. Rdf, 5 ± 1.2%; p < 0.05). Addition of ASCs to Df (Crdf) partially restored its regenerative potential. Aff and Crdf exhibited rapid angiogenesis and M2-polarized macrophages infiltration, in contrast to impaired angiogenesis and M1-polarized inflammatory pattern in Df. GFP + ASCs participated in angiogenesis and displayed a phenotype of endothelial cells in Crdf.Conclusion: Adipose ECM and microenvironment have the capacity to stimulate early adipogenesis while ECM alone cannot induce adipogenesis in vivo. By directly differentiating into endothelial cells and regulating macrophage polarization, ASCs coordinate early adipogenesis with angiogenesis and tissue remodeling, leading to better long-term retention and greater tissue integrity.

scholarly journals Comparison of Microfat, Nanofat and Extracellular Matrix/Stromal Vascular Fraction Gel for Skin Rejuvenation: Basic Research and Clinical Applications

2021 ◽  
Author(s):  
Zhibin Yang ◽  
Shengyang Jin ◽  
Yu He ◽  
Xinyu Zhang ◽  
Xuefeng Han ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Therapeutic Potential ◽  
Stromal Vascular Fraction ◽  
Basic Research ◽  
Sem Analysis ◽  
Clinical Applications ◽  
Fat Grafting ◽  
Histological Structure ◽  
Skin Rejuvenation ◽  
Uniform Dispersion

Abstract Background Over the past two decades, fat grafting has been extensively applied in the field of tissue regeneration. Objectives The authors investigated the therapeutic potential of microfat, nanofat and extracellular matrix/stromal vascular fraction gel (SVF-gel) in skin rejuvenation. Methods Microfat was harvested by a cannula with multiple 0.8 mm smooth side holes and processed with a fat stirrer to remove fibers. Nanofat and SVF-gel were prepared according to previously reported methods. We evaluated their structure and viability. Then, stromal vascular fraction (SVF) cells from the three types of samples were isolated and characterized, and the cell viability was compared. Results The microstructure of the three samples showed distinct differences. The microfat group showed a diameter of 100 to 120 .0μmunder the microscope and presented abotryoid shape under Calcein-AM/Propidium iodide (AM/PI) staining. Scanning electron microscopy (SEM) analysis showed that the microfat maintained integral histological structure.In the nanofat group, no viable adipocytes and no normal histological structure were observed, with high levels of free lipids.The SVF-gel group showed uniform dispersion of cells with different sizes and parts of the adipose histological structure. Cell count and culture revealed that the number of viable SVF cells decreased distinctly in the nanofat group compared with the microfat group. In contrast, the number of viable SVF cells in the SVF-gel group increased moderately. Clinical applications with microfat showed marked improvements in skin wrinkles. Conclusions The study showed that the microfat could preserve the integrity of the histological structure and presents the advantages of subcutaneous volumetric restoration and improvement of skin quality in skin rejuvenation compared with the nanofat and SVF-gel.

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