scholarly journals Reference range of naïve T and T memory lymphocyte subsets in peripheral blood of healthy adult

2021 ◽  
Author(s):  
Ying Xia ◽  
Aqing Liu ◽  
Wentao Li ◽  
Yunhe Liu ◽  
Guan Zhang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Reference Values ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Reference Range ◽  
Healthy Adult ◽  
Memory T Cells ◽  
Memory Cell ◽  
Effector Memory

Abstract Naïve T and T memory cell subsets are closely related to immune response and can provide important information for the diagnosis and treatment of immunological and hematological disorders. Lymphocyte compartment undergo dramatic changes during adulthood; age-related reference values derived from healthy individuals are crucial. However, extensively detailed reference values of peripheral blood lymphocytes in whole spectrum of adulthood detected by multi-color flow cytometry on a single platform are rare. 309 healthy adult volunteers were recruited from Tianjin in China. The absolute counts and percentages of CD3+CD4+ T cells, CD3+CD8+ T cells, naïve T cells (Tn), T memory stem cells (Tscm), central memory T cells (Tcm), effector memory T cells (Tem), terminal effector T cells (Tte) were detected by flow cytometry with single platform technologies. Reference range of absolute counts and percentage of T lymphocyte subsets were formulated by different age and gender. The results showed that Tn and Tscm cells, which had stem cell properties, decreased with aging; while, Tcm and Tem increased with aging, which increased from 18 to 64 years old but presented no significant change over the 65 years old. Gender had influence on the fluctuation of lymphocyte subsets, absolute count of CD3+CD8+, CD8+ Tcm, CD8+ Tem in male were higher than those in female. The reference values of percentages and absolute numbers of naïve T and T memory cell subsets can help doctors to understand the immune state of patients and evaluate conditions of prognosis then adjust treatment for patients.

scholarly journals Reference range of naïve T and T memory lymphocyte subsets in peripheral blood of healthy adult

2021 ◽  
Author(s):  
Ying Xia ◽  
Aqing Liu ◽  
Wentao Li ◽  
Yunhe Liu ◽  
Guan Zhang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Reference Values ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Reference Range ◽  
Healthy Adult ◽  
Memory T Cells ◽  
Memory Cell ◽  
Effector Memory

Abstract: Background： Naïve T and T memory cell subsets can provide important information for the diagnosis and treatment of immunological and hematological disorders. Lymphocyte compartment undergo dramatic changes during adulthood; age-related reference values derived from healthy individuals are crucial. However, extensively detailed immunophenotyping reference values of peripheral blood lymphocytes in whole spectrum of adulthood performed by flow cytometry-based single-platform method are rare. Methods: 309 healthy adult volunteers were recruited from Tianjin in China. The absolute counts and percentages of CD3+CD4+ T cells, CD3+CD8+ T cells, naïve T cells (Tn), T memory stem cells (Tscm), central memory T cells (Tcm), effector memory T cells (Tem), terminal effector T cells (Tte) were determined by flow cytometry with single platform technologies. Results: Reference range of absolute counts and percentage of lymphocyte subsets were formulated by different age and gender. We also find out the changing regularity of them: the cells which have stem cell properties, Tn and Tscm cells, decrease with aging; memory cell subsets, Tcm and Tem increase with aging, which increase from 18 to 64 years old and present no significant change over the 65 years old. Gender have influence on the fluctuation of lymphocyte subsets, absolute count of CD3+CD8+, CD8+ Tcm, CD8+ Tem in male are higher than that in female. Conclusion: The reference values of percentages and absolute numbers of naïve T and T memory cell subsets can help doctors to understand the immune state of patients and to evaluate conditions of prognosis then adjust treatment for patients.

Sezary Syndrome Is a Malignancy of Central Memory T Cells with Skin Homing Properties, While Mycosis Fungoides Is a Malignancy of Skin Homing Effector Memory T Cells.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 3954-3954 ◽  
Author(s):  
James Campbell ◽  
Rachael A Clark ◽  
Thomas S. Kupper
Keyword(s):  
T Cells ◽  
Mycosis Fungoides ◽  
Peripheral Blood ◽  
Memory T Cells ◽  
Memory Cell ◽  
Central Memory ◽  
Effector Memory ◽  
Lesional Skin ◽  
Skin Homing ◽  
Low Levels

Abstract Abstract 3954 Poster Board III-890 The relationship between leukemic CTCL (L-CTCL), which includes Sezary Syndrome (SS), and mycosis fungoides (MF), a CTCL variant characterized by infiltration of skin, is incompletely understood. In the present study, we examined skin and/or peripheral blood from more than 300 patients with diagnoses of CTCL accrued over a five year period. We identified a subset of patients with leukemic CTCL, all of whom had absolute CD4 counts >2000, CD4/CD8 ratios of > 10, and other features of SS (loss of CD7 and/or CD26). Using specific TCR-Vb antibodies, we could identify malignant clonal populations from CD4+/CD8- peripheral blood mononuclear cells of 12 L-CTCL patients, and performed comprehensive analysis of the malignant clones by flow cytometry. With regard to skin-associated trafficking markers, we found consistently high CCR4 expression, but very heterogeneous expression of CCR6, CCR10 and CLA. Lymph-node homing molecules CCR7 and L-selectin were consistently expressed at high levels on the malignant clonal cells. CD45RA-to-CD45RO ratios, which typically distinguish between non-malignant naïve and antigen-experienced CD4 T cells, were unexpectedly variable from patient to patient. However, CD27, whose expression is lost in normal T cells upon effector/memory cell differentiation, was highly expressed by all malignant clonal cells in SS/L-CTCL. We compared this pattern to the expression of homing and maturation markers on malignant cells extracted from lesional skin of patients with MF. These cells reliably expressed CLA and CCR4, but expressed very low levels of CCR7 and L selectin. In addition, they expressed low levels of CD27. Based on this evidence, L-CTCL/SS appears to be a T central-memory cell CD4-lymphocyte malignancy that, with the exception of CCR4, has variable expression of the putative skin-homing markers CLA, CCR6, or CCR10. This is in direct contrast to MF, wherein clonal T cells in lesional skin bear uniform expression of some skin-homing markers but low expression of central memory markers. These data support a growing body of evidence that SS/L-CTCL and MF are fundamentally different malignancies, rather than different clinical stages of a single disease. Disclosures: No relevant conflicts of interest to declare.

Flow Cytometric Detection of the Expression of CXCR4 on CD34+ Cells and the Distribution of Lymphocyte Subsets in Allogeneic Hematological Grafts.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 5195-5195
Author(s):  
Lulu Lu ◽  
Yongping Song ◽  
Baogen Ma ◽  
Xiongpeng Zhu ◽  
Xudong Wei ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
T Cells ◽  
Cord Blood ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Color Flow ◽  
Hematopoietic Stem ◽  
Cd34 Cells ◽  
Mobilized Peripheral Blood

Abstract Background and objectives: Normal human bone marrow (BM), cord blood (CB) and mobilized peripheral blood (MPB) are the most commonly used sources for allogeneic hematopoietic stem cell transplantation (HSCT). The aim of this study was to detect the expression of CXCR4 on CD34+ cells and to assess the distribution of lymphocyte subsets in each type allograft. Methods: CD34+ cells were separated from BM (n=30), CB (n=30) and MPB (n=30) by the CD34 MultiSort Kit immunomagnetic bead system. The expression of CXCR4 on CD34+cells was assayed by double color flow cytometry. The lymphocyte subsets in each type of allograft were detected by three-color flow cytometry. The groups of monoclonal antibodies were used as the following: CXCR4-PE/CD34−Pecy5, CD8−FITC/CD4−R-PE/CD3−TC, CD45RA-FITC/CD45RO-PE/CD4−Pecy5, CD45RA-FITC/CD45RO-PE/CD8−Pecy5, and CD3−FITC/CD16+56-PE. Isotype-specific antibodies were used as controls. Results: The expression of CXCR4 of cord blood and mobilized peripheral blood CD34+ cells was lower than that of bone marrow cells (BM 40.21%±6.72%, CB 20.93%±3.96%, MPB 20.93%±3.96%, P <0.05). The difference between cord blood and mobilized peripheral blood was not significant (P>0.05). The CD3+CD8low and CD3+CD4−CD8low subsets were higher in BM than that of CB and MPB (BM 8.61%±1.40%, CB 3.31%±0.88%, MPB 5.11%±0.76%,P<0.01). The relative frequencies of the naïve CD45RA+ CD45RO− phenotype among CD4+ and CD8high T cells were highest in CB, and it was higher in MPB than in BM grafts (BM 28.09%±4.52%, 41.86 %±3.31%; CB83.83%±12.24%, 86.69%±6.12%; MPB 43.58%±4.54%, 57.64%±4.77%, P<0.01). Naïve T cells (CD45RA+ CD45RO−) were mobilized preferentially compared to memory T cells (CD45RA− CD45RO+)(P <0.01); The relative frequencies of NKT (CD3+CD16+56+) among lymphocytes were lower in CB than that in BM and MPB (CB 0.77±0.19, BM4.15±1.10, MPB 4.13±0.84, P<0.01). Conclusion: BM, CB and MPB allografts differ widely in cellular makeup of CD34+ cells and lymphocyte subsets, which are associated with the distinct characteristics after allogeneic HSCT from different allogeneic hematological sources.

scholarly journals Dynamic Differentiation of Activated Human Peripheral Blood CD8+ and CD4+ Effector Memory T Cells

2005 ◽  
Vol 175 (3) ◽  
pp. 1433-1439 ◽  
Author(s):  
Jochen Schwendemann ◽  
Carmen Choi ◽  
Volker Schirrmacher ◽  
Philipp Beckhove
Keyword(s):  
T Cells ◽  
Peripheral Blood ◽  
Memory T Cells ◽  
Human Peripheral Blood ◽  
Effector Memory ◽  
Effector Memory T Cells

Influence of Race, Age and Sex on the Lymphocyte Subsets in Peripheral Blood of Healthy Malaysian Adults

1995 ◽  
Vol 32 (6) ◽  
pp. 532-539 ◽  
Author(s):  
M L Choong ◽  
S H Ton ◽  
S K Cheong
Keyword(s):  
T Cells ◽  
B Cells ◽  
Nk Cells ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Reference Range ◽  
Suppressor Cells ◽  
Skewed Distribution ◽  
Cd4 Cells ◽  
Cd8 Cells

The lymphocyte subsets in the peripheral blood of healthy Malaysian adults (212 subjects, age 18–71 years) were analysed using a flow cytometer FACScan in an effort to establish a reference range for the lymphocyte subsets. The lymphocyte subsets studied were T cells (CD3), B cells (CD19), natural killer (NK) cells (CD3−CD16+/CD56+), helper/inducer cells (CD4), cytotoxic/suppressor cells (CD8) and the helper/suppressor ratio (CD4/CD8). The distributions of T cells, CD4 cells and CD8 cells were symmetric about their means while B cells, NK cells and CD4/CD8 ratio followed a skewed distribution. Differences in race were observed for T cells, NK cells, CD4 cells and CD4/CD8 ratio where the Indians were significantly different from the Malays and the Chinese (higher T cells, CD4 cells and CD4/CD8 ratio and lower NK cells). The B cells were significantly lower in the Chinese than the Malays and the Indians. Age differences were seen only in the Chinese where increased CD4 cells and CD4/CD8 ratio, and decreased CD8 cells were observed. A sex difference was observed only in the Chinese where the CD4/CD8 ratio was significantly higher in females than males.

scholarly journals Peripheral Blood CD38 Bright CD8+ Effector Memory T Cells Predict Acute Graft-versus-Host Disease

2015 ◽  
Vol 21 (7) ◽  
pp. 1215-1222 ◽  
Author(s):  
Pooja Khandelwal ◽  
Adam Lane ◽  
Vijaya Chaturvedi ◽  
Erika Owsley ◽  
Stella M. Davies ◽  
...  
Keyword(s):  
T Cells ◽  
Graft Versus Host Disease ◽  
Peripheral Blood ◽  
Memory T Cells ◽  
Effector Memory ◽  
Host Disease ◽  
Graft Versus Host ◽  
Effector Memory T Cells ◽  
Acute Graft

scholarly journals Establishment of Adult Peripheral Blood Lymphocyte Subset Reference Range for an Asian Population by Single-Platform Flow Cytometry: Influence of Age, Sex, and Race and Comparison with Other Published Studies

2004 ◽  
Vol 11 (1) ◽  
pp. 168-173 ◽  
Author(s):  
Wee J. Chng ◽  
Guat B. Tan ◽  
Ponnudurai Kuperan
Keyword(s):  
Flow Cytometry ◽  
Peripheral Blood Lymphocyte ◽  
Peripheral Blood ◽  
Blood Lymphocyte ◽  
Lymphocyte Subsets ◽  
Reference Range ◽  
Nk Cell ◽  
Published Data ◽  
Reference Ranges ◽  
Cell Counts

ABSTRACT We established a normal reference range for peripheral blood lymphocyte subsets in a multiracial adult population by using single-platform flow cytometry. Further analysis of our cohort showed that the CD8+-cell counts decrease with age, there is a gender difference in NK cell percentages and counts, and there are significant differences in the CD3+-, CD4+-, and CD19+-cell counts between Indians and other racial groups. Overall, our results are significantly different from other published data. This difference further stresses the need for different populations to establish their own reference ranges as these may have important implications for the management of patients with human immunodeficiency virus and AIDS. The use of single-platform flow cytometry will eliminate some of the variability between different study centers, making studies more comparable. This platform should be used for future studies into the effects of age, sex, and race on lymphocyte subsets.

scholarly journals Interferon regulatory factor 4 controls effector functions of CD8+ memory T cells

2021 ◽  
Vol 118 (16) ◽  
pp. e2014553118
Author(s):  
Aenne Harberts ◽  
Constantin Schmidt ◽  
Joanna Schmid ◽  
Daniel Reimers ◽  
Friedrich Koch-Nolte ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Population ◽  
T Cell Activation ◽  
Cell Activation ◽  
Memory T Cells ◽  
Memory Cell ◽  
Effector Memory ◽  
Effector Cells ◽  
Effector Functions

The transcription factor IRF4 is required for CD8+ T cell activation, proliferation, and differentiation to effector cells and thus is essential for robust CD8+ T cell responses. The function of IRF4 in memory CD8+ T cells yet needs to be explored. To investigate the role of IRF4 for maintaining differentiation state and survival of CD8+ memory T cells, we used a mouse model with tamoxifen-inducible Irf4 knockout to preclude effects due to inefficient memory cell differentiation in absence of IRF4. We infected mice with ovalbumin-recombinant listeria and induced Irf4 knockout after clearance of the pathogen. Loss of IRF4 resulted in phenotypical changes of CD8+ memory T cells but did not cause a reduction of the total memory T cell population. However, upon reencounter of the pathogen, CD8+ memory T cells showed impaired expansion and acquisition of effector functions. When compared to CD8+ effector memory T cells, CD8+ tissue-resident memory T cells (TRM cells) expressed higher IRF4 levels. Mice with constitutive Irf4 knockout had diminished CD8+ TRM-cell populations, and tamoxifen-induced Irf4 deletion caused a reduction of this cell population. In conclusion, our results demonstrate that IRF4 is required for effective reactivation but not for general survival of CD8+ memory T cells. Formation and maintenance of CD8+ TRM cells, in contrast, appear to depend on IRF4.

scholarly journals Identifying Changes in Peripheral Lymphocyte Subpopulations in Adult Onset Type 1 Diabetes

2021 ◽  
Vol 12 ◽  
Author(s):  
Aina Teniente-Serra ◽  
Eduarda Pizarro ◽  
Bibiana Quirant-Sánchez ◽  
Marco A. Fernández ◽  
Marta Vives-Pi ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cells ◽  
Type 1 Diabetes ◽  
B Cells ◽  
Peripheral Blood ◽  
Disease Onset ◽  
Lymphocyte Subpopulations ◽  
Effector Memory ◽  
Transitional B Cells

T- and B-lymphocytes play an important role in the pathogenesis of type 1 diabetes (T1D), a chronic disease caused by the autoimmune destruction of the insulin-producing cells in the pancreatic islets. Flow cytometry allows their characterization in peripheral blood, letting to investigate changes in cellular subpopulations that can provide insights in T1D pathophysiology. With this purpose, CD4+ and CD8+ T cells (including naïve, central memory, effector memory and terminally differentiated effector (TEMRA), Th17 and Tregs) and B cells subsets (naïve, unswitched memory, switched memory and transitional B cells) were analysed in peripheral blood of adult T1D patients at disease onset and after ≥2 years using multiparametric flow cytometry. Here we report changes in the percentage of early and late effector memory CD4+ and CD8+ T cells as well as of naïve subsets, regulatory T cells and transitional B cells in peripheral blood of adult patients at onset of T1D when compared with HD. After 2 years follow-up these changes were maintained. Also, we found a decrease in percentage of Th17 and numbers of T cells with baseline. In order to identify potential biomarkers of disease, ROC curves were performed being late EM CD4 T cell subset the most promising candidate. In conclusion, the observed changes in the percentage and/or absolute number of lymphocyte subpopulations of adult T1D patients support the hypothesis that effector cells migrate to the pancreas and this autoimmune process perseveres along the disease. Moreover, multiparametric flow allows to identify those subsets with potential to be considered biomarkers of disease.

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