Rapid High-Performance Liquid Chromatography Method for Levodopa Quantitation at Low UV Wavelength: Application of Pharmacokinetics Study in Rat Following Intranasal Delivery

Liquid Chromatography ◽

Residence Time ◽

High Performance ◽

Hplc Method ◽

Pharmacokinetic Parameters ◽

Intranasal Delivery ◽

Suitable Alternative ◽

Thermosensitive Gel ◽

Abstract Levodopa is widely administered orally in clinical treatment of Parkinson’s disease; however, due to levodopa various oral absorption and low bioavailability, intranasal delivery seems to be a suitable alternative route of administration. Pluronic F-127 is a thermosensitive polymer, which can form gel at nasal cavity temperature and increase drug residence time. In this study, a rapid High Performance Liquid Chromatography (HPLC) method was validated in presence of internal standard to determine pharmacokinetic parameters following levodopa administration to rats in three different intravenous solution, intranasal solution and intranasal thermosensitive gel groups. A precised (96.7%) and accurate (95.0%) HPLC method was validated at low UltraViolet (UV) wavelength of 208 nm that showed limit of detection and limit of quantitation of 59 and 177 ng/mL, respectively. Specificity results showed no interference for levodopa with endogenous serum materials, and serum extraction efficacy was 93%. Pharmacokinetic parameters including bioavailability of 75 and 85% with mean residence time of 78 and 94 min were estimated for intranasal solution and thermosensitive gel using the validated HPLC method, which indicated that levodopa nasal gel may be a good alternative with appropriate pharmacokinetic outcome. Therefore, the validated levodopa HPLC analysis method at low UV wavelength was efficiently applied in pharmacokinetic study.

Intravascular Residence Time Determination for the Cyanide Antidote Dimethyl Trisulfide in Rat by Using Liquid-Liquid Extraction Coupled with High Performance Liquid Chromatography

Journal of Analytical Methods in Chemistry ◽

10.1155/2016/6546475 ◽

2016 ◽

Vol 2016 ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

Deepthika De Silva ◽

Steven Lee ◽

Anna Duke ◽

Siva Angalakurthi ◽

Ching-En Chou ◽

...

Keyword(s):

Liquid Chromatography ◽

Residence Time ◽

Rat Model ◽

High Performance ◽

Liquid Extraction ◽

Dimethyl Trisulfide ◽

Liquid Liquid Extraction ◽

Cyanide Antidote ◽

These studies represent the first report on the intravascular residence time determinations for the cyanide antidote dimethyl trisulfide (DMTS) in a rat model by using high performance liquid chromatography coupled with ultraviolet absorption spectroscopy (HPLC-UV). The newly developed sample preparation included liquid-liquid extraction by cyclohexanone. The calibration curves showed a linear response for DMTS concentrations between 0.010 and 0.30 mg/mL withR2= 0.9994. The limit of detection for DMTS via this extraction method was 0.010 mg/mL, and the limit of quantitation was 0.034 mg/mL. Thus this calibration curve provided a tool for determining DMTS in the range between 0.04 and 0.30 mg/mL. Rats were given 20 mg/kg DMTS dose (in 15% Polysorbate 80) intravenously, and blood samples were taken 15, 60, 90, 120, and 240 min after DMTS injections. The data points were plotted as DMTS concentration in RBCs versus time, and the intravascular residence time was determined graphically. The results indicated a half-life of 36 min in a rat model, suggesting that the circulation time is long enough to provide a reasonable time interval for cyanide antagonism.

Establishment of an HPLC Method for Determination of Coumarin-3-Carboxylic Acid Analogues in Rat Plasma and a Preliminary Study on Their Pharmacokinetics

Journal of Chromatographic Science ◽

10.1093/chromsci/bmab103 ◽

2021 ◽

Author(s):

Yan Xiong ◽

Yong-Hong Liu ◽

Jian-Sha Li ◽

Yu-Ying Zhang ◽

Jing Zhang ◽

...

Keyword(s):

Liquid Chromatography ◽

Carboxylic Acid ◽

High Performance ◽

Rat Plasma ◽

Hplc Method ◽

Array Detector ◽

Pharmacokinetic Parameters ◽

Preliminary Study

Abstract A simple high performance liquid chromatography (HPLC) method was developed and validated for the determination of coumarin-3-carboxylic acid analogues (C3AA) in rat plasma and a preliminary study on pharmacokinetics. Ferulic acid (FA) was used as the internal standard substance, and coumarin-3-carboxylic acid (C3A) was used as a substitute for quantitative C3AA. After protein precipitation with methanol, the satisfactory separation was achieved on an ODS2 column when the temperature was maintained at 30 ± 2°C. The correlation coefficient r in the C3A linear equation is equal to 0.9990. Pharmacokinetic parameters for t1/2, Tmax, Cmax, area under the curve (AUC)0-t, average residence time (MRT), apparent volume of distribution (V z/F) and clearance (Cl/F) were 1.89 ± 0.03 h, 0.39 ± 0.14 h, 1.81 ± 0.10 g· mL−1 ·h, 7.88 ± 0.24 g·mL−1·h, 3.23 ± 0.14 h, 0.43 ± 0.03 (mg·kg−1)·(g·mL−1)−1·h−1, respectively. The high performance liquid chromatography-photo diode array detector (HPLC-PDA) method established in this study can be used to separate and determine the content of C3AA in plasma of rats after 60% ethanol extraction by gavage. The plasma concentration-time curve and pharmacokinetic parameters reflect the absorption of C3AA in rat blood after oral administration to some extent.

Development and Validation of Stability-Indicating Reverse Phase-High Performance Liquid Chromatography Method for Simultaneous Determination of Atenolol and Nifedipine in Bulk and Tablet Dosage Form

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.11.2.4 ◽

2020 ◽

Vol 11 (02) ◽

pp. 219-223

Author(s):

Ansari Yaasir Ahmed ◽

Qazi Shoeb ◽

Umme Rumana ◽

Patel Afroza ◽

Pathan Vahid Tajkhan ◽

...

Keyword(s):

Liquid Chromatography ◽

High Performance ◽

Dosage Form ◽

Limit Of Detection ◽

Hplc Method ◽

Forced Degradation ◽

Chromatography Method ◽

Stability Indicating ◽

The new stability-indicating high performance liquid chromatography (HPLC) method has been developed and validated with different parameters for atenolol (ATE) and nifedipine (NIFE) in the combined dosage form. The chromatographic conditions were optimized using a mobile phase of MeOH:OPA (70:30) with a flow rate of 0.7 mL/min. Column (C18) of 4.6 × 250 mm dimension was used as a stationary phase; the particle size capacity of the column was 5 μm. The detection was carried out at 233 nm. The method was validated according to ICH guidelines for linearity, precision, repeatability, the limit of detection (LoD), and limit of quantitation (LoQ). The response was found to be linear in the concentration range of 20 to 100 mcg/mL for ATE and 1 to 5 mcg/mL for NIFE. The developed method shows the minimum quantity of drugs to be identified (LoD) and minimum drug to be quantified (LoQ). The LoD and LoQ were found to be 0.1415 and 0.4289, respectively, for ATE, and 0.1834 and 0.5558, respectively, for NIFE. The method was linear, simple, precise, and accurate and, therefore, suitable for routine analysis of drugs in tablet form. The forced degradation studies were also done through the exposure of analyte solution to four different stress conditions.

A NEW RP-HPLC METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF KETOROLAC TROMETHAMINE AND TRAMADOL HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORMS.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i5.15976 ◽

2017 ◽

Vol 10 (5) ◽

pp. 186

Author(s):

Bharathi Devi Y ◽

Sumanth Srinivas Kamatham ◽

Bhaskara Raju V ◽

Mohan Gandhi B ◽

Srinivas K

Keyword(s):

Liquid Chromatography ◽

High Performance ◽

Reversed Phase ◽

Hplc Method ◽

Simultaneous Estimation ◽

Ketorolac Tromethamine ◽

Tramadol Hydrochloride ◽

Relative Standard ◽

Objective: This study was embarked upon to develop a new, simple, rapid, validated reversed-phase high-performance liquid chromatography (HPLC)method for the estimation of ketorolac tromethamine (KET) and tramadol hydrochloride (TDL) in pharmaceutical dosage forms.Methods: The HPLC method was developed on Shishiedo C18 column (250 mm × 4.6 mm i.d, 5 μ) using methanol: 50 mM phosphate buffer (pH 6.0) in the ratio of 52:48 at 282 nm.Results: Retention time for the drugs was found to be 5.1 and 6.9 minutes for tramadol and ketorolac, respectively. The limit of detection for tramadoland ketorolac were found to be 1.0 and 0.1 μg/ml, limit of quantitation for tramadol and ketorolac were found to be 5.0 and 0.5 μg/ml, respectively. Linearity was established in the range of 20.0-30.0 μg/ml and 8.0-12.0 μg/ml for TDL and KET, respectively. The method was precise with % relative standard deviation <2 for both intra- and interday precision. The accuracy of the method was performed over three levels of concentration, and the recovery was in the range of 98-102%.Conclusion: From the found experimental data, it can be concluded that the developed method is accurate, precise, and selective and can be employedsuccessfully for the estimation of KET and TDL in Pharmaceutical dosage forms.Keywords: Reversed-phase high-performance liquid chromatography, Ketorolac tromethamine, Tramadol hydrochloride.

Analysis of Piperine in Black Pepper by High Performance Liquid Chromatography

Journal of Nepal Chemical Society ◽

10.3126/jncs.v41i1.30492 ◽

2020 ◽

Vol 41 (1) ◽

pp. 80-86

Author(s):

Suraj Shrestha ◽

Nibedita Chaudhary ◽

Rajkumari Sah ◽

Neera Malakar

Keyword(s):

Liquid Chromatography ◽

High Performance ◽

Limit Of Detection ◽

Accurate Method ◽

Black Pepper ◽

Hplc Method ◽

Limit Of Quantitation ◽

Precision Limit

Piperine is the most important alkaloid present in the black pepper. A high performance liquid chromatography (HPLC) method proposed by International Organization for Standardization (ISO) was optimized and validated for quantitative determination of piperine in black pepper. The method was successfully validated for linearity, range, accuracy, precision, limit of detection, limit of quantitation, and measurement uncertainty. The validated method was used for the analysis of nine black pepper samples collected from the local supermarket of Kathmandu. The piperine content of the samples collected from various areas of Kathmandu, Nepal were found in the range of 2.33 % to 3.34 % with an average of 2.75 % and a standard deviation of 0.31%. This simple, precise, and accurate method can be used for routine analysis of piperine content in black pepper for quality control purposes.

The Effect of Omeprazole on the Pharmacokinetics of Piroxicam Profiles with High Performance Liquid Chromatography (HPLC) Method

Indonesian Journal of Pharmaceutical and Clinical Research ◽

10.32734/idjpcr.v3i1.4049 ◽

2020 ◽

Vol 3 (1) ◽

pp. 36-41

Author(s):

Firdaus Fahdi ◽

Herviani Sari ◽

Sulasmi ◽

Wahyudi

Keyword(s):

Experimental Study ◽

Liquid Chromatography ◽

High Performance ◽

Pharmacokinetic Profile ◽

Hplc Method ◽

Pharmacokinetic Parameters ◽

Accuracy And Precision ◽

Absorption Phase ◽

Parameter Values

Piroxicam is an anti-inflammatory drug of the NSAID class that is commonly used as an analgesic and anthireumatic drug. Its use is often combined with gastric drugs, one of which is omeprazole, considering the side effects of piroxicam which can irritate the stomach. Piroxicam and omeprazole work on the same enzyme, CYP-450 so that it can affect the pharmacokinetic profile especially in the metabolic and excretion phases of piroxicam. This research is an experimental study using 3 male rabbits. Measurement of plasma levels of piroxicam drug is carried out using a High Performance’s Liquid Chromatography (HPLC) tool. The validation method is used to determine LOD and LOQ values, accuracy, and precision tests. The results of data analysis using t-tables. The results showed the value of pharmacokinetic parameters in the absorption phase increased or there were differences but did not show any significant effect on each group. While the pharmacokinetic parameter values in the metabolic and excretion phases decreased and showed no differences between groups. The administration of omeprazole and piroxicam together and the administration of piroxicam which was given an hour earlier by omeprazole can inhibit the enzyme that stimulates the metabolism of piroxicam so that it affects the pharmacokinetic parameters in the absorption phase and also excretion but is not significant.

Developing a High-performance Liquid Chromatography Method for Simultaneous Determination of Loratadine and its Metabolite Desloratadine in Human Plasma

Current Drug Metabolism ◽

10.2174/1389200220666191125095648 ◽

2020 ◽

Vol 20 (13) ◽

pp. 1053-1059

Author(s):

Mahmoud M. Sebaiy ◽

Noha I. Ziedan

Keyword(s):

Liquid Chromatography ◽

Human Plasma ◽

High Performance ◽

Allergic Diseases ◽

Reversed Phase ◽

Hplc Method ◽

H1 Receptor ◽

Chromatography Method

Background: Allergic diseases are considered as the major burden on public health with increased prevalence globally. Histamine H1-receptor antagonists are the foremost commonly used drugs in the treatment of allergic disorders. The target drug in this study, loratadine, belongs to this class of drugs and its biometabolite desloratadine which is also a non-sedating H1 receptor antagonist with anti-histaminic activity being 2.5 to 4 times greater than loratadine. This study aimed to develop and validate a novel isocratic Reversed-phase High-Performance Liquid Chromatography (RP-HPLC) method for rapid and simultaneous separation and determination of loratadine and its metabolite, desloratadine in human plasma. Methods: The drug extraction method from plasma was based on protein precipitation technique. The separation was carried out on a Thermo Scientific BDS Hypersil C18 column (5μm, 250 x 4.60 mm) in a mobile phase of MeOH: 0.025M KH2PO4 adjusted to pH 3.50 using orthophosphoric acid (85: 15, v/v) at an ambient temperature. The flow rate was maintained at 1 mL/min and maximum absorption was measured using the PDA detector at 248 nm. Results: The retention times of loratadine and desloratadine in plasma samples were recorded to be 4.10 and 5.08 minutes, respectively, indicating a short analysis time. Limits of detection were found to be 1.80 and 1.97 ng/mL for loratadine and desloratadine, respectively, showing a high degree of sensitivity of the method. The method was then validated according to FDA guidelines for the determination of the two analytes in human plasma. Conclusion: The results obtained indicate that the proposed method is rapid, sensitive in the nanogram range, accurate, selective, robust and reproducible compared to other reported methods.

HPLC Analysis of Oxytetracycline Residues in Honey

Journal of Food Protection ◽

10.4315/0362-028x-49.5.383 ◽

1986 ◽

Vol 49 (5) ◽

pp. 383-388 ◽

Cited By ~ 19

Author(s):

PETER SPORNS ◽

SUET KWAN ◽

LAWRENCE A. ROTH

Keyword(s):

Liquid Chromatography ◽

Aqueous Solutions ◽

High Performance ◽

Hplc Analysis ◽

Extraction Procedure ◽

Hplc Method ◽

Limits Of Detection ◽

Ph Values ◽

Double Extraction

Oxytetracycline (OTC), also known commercially as Terramycin, was determined to be more stable in honey than in buffered aqueous solutions at similar pH values and temperatures. A rapid high performance liquid chromatography (HPLC) method was developed to detect and quantitate OTC using a 1:1 dilution (wt/wt) of honey samples in water. Using 355 nm as the wavelength of detection, amounts as low as 0.5 μg/ml could be detected in the above solution. The limits of detection were lowered considerably by a double extraction procedure.

A Selective High-Performance Liquid Chromatography Method for the Determination of Reboxetine in Bulk Drug and Tablets

Journal of AOAC International ◽

10.1093/jaoac/89.6.1552 ◽

2006 ◽

Vol 89 (6) ◽

pp. 1552-1556

Author(s):

ArmaĞan Önal ◽

Olcay SaĞiri ◽

S Müge Çetin ◽

Sidika Toker

Keyword(s):

Liquid Chromatography ◽

High Performance ◽

Depressive Disorders ◽

Degradation Products ◽

Severe Depression ◽

Hplc Method ◽

Chromatography Method ◽

Relative Standard

Abstract Reboxetine is used as a selective noradrenaline reuptake inhibitor for the treatment of major depressive disorders. It is effective in the treatment of severe depression and safer to use than traditional tricyclic antidepressants. In this study, a novel, simple, and rapid stability-indicating high-performance liquid chromatography (HPLC) method for reboxetine methansulfonate was successfully developed and validated for the assay of tablets. The method was used to quantify reboxetine in tablets; it employed a C18 column (150 4.6 mm id) with an isocratic mobile phase consisting of methanolphosphate buffer (pH 7, 0.02 M; 55 + 45, v/v) at a flow rate of 1.0 μmL/min. Reboxetine was detected by an ultraviolet detector at 277 nm. The retention time of reboxetine was about 4.5 min. The developed HPLC method was validated with respect to linearity, precision, sensitivity, accuracy, and selectivity. The method was linear over the concentration range 150 g/mL (r 0.9999). The limits of detection and the quantitation of reboxetine were 0.1 and 0.3 μg/mL, respectively. The relative standard deviation values for intraday and interday precision were 0.781.01 and 1.081.37%, respectively. Selectivity was validated by subjecting a stock solution of reboxetine to neutral, acid, and alkali hydrolysis, as well as oxidation, dry heat treatment, and photodegradation. The peaks of the degradation products did not interfere with the peak of reboxetine. The results indicated that the proposed method could be used in a stability assay. The proposed method was successfully applied to the determination of reboxetine in tablets. Excipients present in the tablets did not interfere with the analysis.

Development of reverse phase-high performance liquid chromatography (RP-HPLC) method for determination of selected antihypertensive active flavonoids (rutin, myricetin, quercetin, and kaempferol) in medicinal plants found in Botswana

Physical Sciences Reviews ◽

10.1515/psr-2020-0209 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Katso Binang ◽

David T. Takuwa

Keyword(s):

Liquid Chromatography ◽

Medicinal Plants ◽

High Performance ◽

Zingiber Officinale ◽

Hplc Method ◽

Reverse Phase ◽

Rp Hplc ◽

Lippia Javanica

Abstract The aim of the study was to develop a rapid, efficient, and cheap chromatographic method for determining four selected antihypertensive active flavonoid compounds in medicinal plants in Botswana. The determination of rutin, quercetin, and kaempferol in selected medicinal plants was conducted in less than 6 min using the developed reverse phase-high performance liquid chromatography (RP-HPLC) method with a 2.7 µm Ascentis C18 express column (150 × 4.60 mm i.d) at 340, 360, and 368 nm detection wavelengths and mobile phase of methanol and 0.068% of formic acid solution in isocratic elution. Validation results showed good selectivity, linearity (r 2 > 0.99), high percentage recoveries (90.2–104.7%), and precision (% RSD < 2) for n = 3, confirming suitability of the method for determination of the investigated flavonoids in Zingiber officinale (ginger). Application of the developed RP-HPLC method was performed in selected medicinal plants (Lippia javanica ) (mosukujane), Myrothanmus flabellious (galalatshwene), and Elephantorrhiza elephantina (mositsana) used to manage hypertension by herbalists in Botswana. M. flabellious a very commonly used plant for managing hypertension was found to contain highest amounts of rutin and myricetin, whereas nothing was detected for E. elephantina.