Factors Affecting the Radioimmunoassay of Digoxin

1972 ◽  
Vol 18 (6) ◽  
pp. 539-543 ◽  
Author(s):  
Eugene Cerceo ◽  
Cipriano A Elloso
Keyword(s):  
Blood Plasma ◽  
Diagnostic Tests ◽  
Plasma Proteins ◽  
Sample Collection ◽  
Factors Affecting ◽  
Uremic Patients ◽  
In Blood Plasma

Abstract We ascertained which factors critically affect the radioimmunoassay of digoxin. These include: products of hemolysis in blood plasma, excessive amounts of bilirubin in the plasma of jaundiced patients, the age of the tritiated digoxin, the presence of gamma-emitting radioisotopes in the plasma from diagnostic tests, the binding tendency of plasma proteins for digoxin, chemiluminescence associated with the serum (or plasma) of uremic patients, and excessive delay between sample collection and assay of unfrozen samples.

The influence of alkylation on the photophysical properties of BODIPYs and their labeling in blood plasma proteins

2020 ◽  
Vol 304 ◽  
pp. 112717 ◽  
Author(s):  
Lyubov A. Antina ◽  
Alexander A. Ksenofontov ◽  
Alexander A. Kalyagin ◽  
Pavel S. Bocharov ◽  
Nadezhda V. Kharitonova ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Plasma Proteins ◽  
Photophysical Properties ◽  
Blood Plasma Proteins ◽  
In Blood Plasma

scholarly journals Protective action of carnosine and organic lithium salts in case of ethanol-induced oxidative damage of proteins and lipids of blood plasma in healthy persons and alcoholic patients

2019 ◽  
Vol 65 (1) ◽  
pp. 28-32 ◽  
Author(s):  
V.D. Prokopieva ◽  
E.V. Plotnikov ◽  
E.G. Yarygina ◽  
N.A. Bokhan
Keyword(s):  
Blood Plasma ◽  
Plasma Proteins ◽  
Protective Action ◽  
Lithium Carbonate ◽  
Lithium Salts ◽  
Oxidized Proteins ◽  
Blood Plasma Proteins ◽  
Healthy Persons ◽  
In Blood Plasma

Organic lithium salts containing anionic components (succinate, fumarate, pyruvate and antioxidant ascorbate) were tested for protection of blood plasma proteins and lipids against ethanol-induced oxidation in vitro. We used normothymic lithium carbonate and well-known antioxidant dipeptide carnosine (b-alanyl-L-histidine) as the reference drugs. The oxidized proteins and lipids were determined by the level of carbonylated proteins (CP) and TBA-reactive products (TBA-RP), respectively. In alcoholic patients the level of oxidized proteins and lipids was higher than in healthy persons. Incubation of blood with ethanol resulted in an increase in oxidized proteins and lipids in blood plasma of healthy persons but had no influence on the level of CP and TBA-RP in blood plasma of alcoholic patients. Lithium carbonate, lithium ascorbate, and lithium succinate exhibited protective action against ethanol-induced oxidation of biomolecules of blood plasma of healthy people. These effects were comparable with carnosine action. The studied compounds had no effect on the level of CP and TBA-RP of blood plasma of alcoholic patients.

To the pathogenesis and clinic of nephrosis

1937 ◽  
Vol 33 (2) ◽  
pp. 126-132
Author(s):  
A. G. Teregulov
Keyword(s):  
Uric Acid ◽  
Lipid Metabolism ◽  
Blood Plasma ◽  
Osmotic Pressure ◽  
Protein Metabolism ◽  
Protein Fraction ◽  
Plasma Proteins ◽  
The Body ◽  
Blood Plasma Proteins ◽  
In Blood Plasma

Clinically, with nephrosis, we observe edema, albuminuria, lipoiduria, which, as follows from the data of modern clinics, along with changes in the kidneys, largely depend on the general suffering of the body disorders of protein and lipid metabolism. Indeed, studies have established that with nephrosis there are deep biochemical shifts - a decrease in blood plasma proteins, a change in the ratio of the protein fraction of the blood towards globulins, an increase in the content of fibrinogen, uric acid, a decrease in osmotic pressure and, finally, in parallel with a change in protein metabolism and changes in fat-lipoid metabolism - hypercholesterolemia.

scholarly journals Change in the Microviscosity of Erythrocyte Membranes and Proteins in Blood Plasma after Graphene Oxide Addition: The ESR Spectroscopy Study

2019 ◽  
Vol 2019 ◽  
pp. 1-8
Author(s):  
V. A. Karachevtsev ◽  
N. T. Kartel ◽  
L. V. Ivanov ◽  
A. N. Lyapunov ◽  
O. A. Nardid ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Blood Plasma ◽  
Plasma Proteins ◽  
Spin Probe ◽  
Esr Spectroscopy ◽  
Protein Secondary Structure ◽  
Small Deformation ◽  
Erythrocyte Membranes ◽  
Oxide Addition ◽  
In Blood Plasma

The change in the microviscosity of erythrocyte membranes and the proteins in blood plasma after graphene oxide addition is studied by the ESR spectroscopy exploiting two spin probes with different lipophilic components in the structures. Experiments with charged spin probe 2 embedded into the erythrocyte membrane showed that the introduction of graphene oxide in small concentrations (∼70 μg/ml) into a suspension of erythrocytes did not lead to significant changes in the microviscosity of their membranes. Correlation times of hydrophobic spin probe 1 adsorbed to hydrophobic pockets of plasma proteins demonstrate a gradual slowdown at the graphene oxide injection into blood plasma that indicates a small deformation of the hydrophobic cavity of protein at the adsorption. However, this protein binding with graphene oxide does not cause the displacement of the spin probe from their hydrophobic cavities, which is evidence about small changes in the protein secondary structure. The obtained results indicate the insignificant cytotoxicity effect of small concentrations of graphene oxide for erythrocytes and blood plasma.

scholarly journals Dynamics of changes in lipid metabolism indices in organs and tissues of experimental animals under adrenaline stress

2015 ◽  
Vol 17 (4) ◽  
Author(s):  
S. V. Shkurashivska ◽  
H. M. Erstenyuk
Keyword(s):  
Lipid Metabolism ◽  
Blood Plasma ◽  
Single Injection ◽  
Sample Collection ◽  
Short Term ◽  
Adrenaline Release ◽  
High Level ◽  
Different Tissues ◽  
Adrenaline Injection ◽  
In Blood Plasma

<p>Diseases of human population, especially in the countries with high level of urbanization, are more often associated now with the influence of different stresses, including emotional one, which is accompanied by adrenaline release.<br />It has been investigated the effect of adrenaline on triacylglycerol (TA G) and cholesterol (CL ) content in different tissues of Wistar rats. It has been determined that under short-term adrenaline load (single injection of adrenaline<br />followed by sample collection after 30 min) TA G level in blood plasma ecreased and simultaneously increased in liver. At the same time CL concentration in blood plasma decreased, while increased in liver and muscles. Double injection of adrenaline followed by sample collection after 30 min caused a decrease in TA G concentration in blood plasma and its increase in liver. Twenty four hours after double adrenaline injection it has been observed decreased TA G and CL levels in blood plasma, whereas it has not differed significantly from control in muscles and liver. Such changes of lipid metabolism under adrenaline stress should be taken into account during evaluation of pathologic process in certain organs.</p>

A simple assay method for added stable caesium in blood plasma

2006 ◽  
Vol 94 (1) ◽  
Author(s):  
Matti Kaikkonen ◽  
Jukka Lehto
Keyword(s):  
Blood Plasma ◽  
Plasma Proteins ◽  
Assay Method ◽  
Present Stage ◽  
Inexpensive Method ◽  
Treatment Standard ◽  
Standard Procedures ◽  
Qualitative Detection ◽  
Ammonium Iron ◽  
In Blood Plasma

SummaryA new assay method was developed to determine added stable caesium (Cs) in blood plasma. The simple and inexpensive method is based on the competition between stable and radioactive Cs in binding to ammonium-iron(II)-hexacyanoferrate(III) (AFCF), followed by the precipitation of AFCF-bound Cs with plasma proteins, and the measurement of the radioactivity of the precipitate. For data treatment, standard procedures of radioimmunoassay were used. At present stage, the qualitative detection limit is around 0.2 μmol L

State of hormonal regulation in experimental animals exposed to noise and vibration

2020 ◽  
pp. 680-681
Author(s):  
A. V. Lizarev ◽  
V. A. Pankov
Keyword(s):  
Blood Plasma ◽  
Hormonal Regulation ◽  
Adrenocorticotropic Hormone ◽  
Vibration Exposure ◽  
Noise And Vibration ◽  
Experimental Animals ◽  
In Blood Plasma

When exposed to noise and vibration in experimental animals there was a decrease in the content of threeiodinethyronine, thyroxin and adrenocorticotropic hormone in blood plasma after 15 and 30 days of experience. An increase in loads led to an increase in the level of threeiodinethyronine and thyroxin under vibration exposure and was normalized with noise. The content of adrenocorticotropic hormone leveled in both cases.

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