Measurement of Hexoses and Pentoses, Singly or in Combination, with o-Anisidine
Abstract Hexoses and pentoses can be measured either singly or in combination, with an o-anisidine reagent. It has the advantages of a fast reaction rate and relatively little interference from hexoses (Amax = 660 nm) in the measurement of pentoses. A simple subtraction of absorbance attributable to glucose at the maximum for pentoses (465 nm) is sufficient for satisfactory pentose estimation in urine. The method has been automated, and results compare favorably with those from an automated procedure for the measurement of serum glucose in which hexokinase is used, and also with those from a colorimetric aniline method for measurement of pentoses in urine. Interferences and procedural variables are discussed. Urinary pentose and hexose values for normal infants are reported.