Erythrocyte protoporphyrin/heme ratio in the assessment of iron status.

1979 ◽  
Vol 25 (1) ◽  
pp. 87-92 ◽  
Author(s):  
R F Labbé ◽  
C A Finch ◽  
N J Smith ◽  
R N Doan ◽  
S K Sood ◽  
...  
Keyword(s):  
Iron Status ◽  
Transferrin Saturation ◽  
Molar Ratio ◽  
Confirmatory Test ◽  
Blood Proteins ◽  
Men And Women ◽  
Healthy Men ◽  
The Mean ◽  
Blood Specimens ◽  
Hemin Chloride

Abstract The protoporphyrinemia of iron deficiency is well recognized. Clinically, information on the protoporphyrin/heme molar ratio in whole blood offers certain advantages over protoporphyrin measurement alone. A procedure for determining this ratio is reported. Protoporphyrin is extracted, solubilized, and measured fluorometrically. Heme (as hemin chloride) is precipitated with the blood proteins, the precipitate is dissolved in an alkaline/pyridine solvent, and the resulting bispyridine ferriprotoporphyrin is measured spectrophotometrically. The molar ratio of these two metabolites correlates well with values for plasma ferritin, plasma iron, transferrin saturation, hemoglobin, and hematocrit. In some cases the ratio increases detectably while the other variables, especially hematocrit and hemoglobin, remain normal. Evidently it is a more sensitive index to iron status. For healthy men and women, the mean ratio is 16.0 (SD, 5.3). The mean + 3 SD, or a ratio of 32, is distinctly abnormal, as shown by a confirmatory test. We validated the test by surveying routine blood specimens obtained from several population groups.

Day-to-day variation in iron-status indices in healthy men and women

1991 ◽  
Vol 54 (4) ◽  
pp. 729-735 ◽  
Author(s):  
M J Borel ◽  
S M Smith ◽  
J Derr ◽  
J L Beard
Keyword(s):  
Iron Status ◽  
Men And Women ◽  
Healthy Men

Effects of bathing, passive range-of-motion exercises, and turning on oxygen consumption in healthy men and women

1994 ◽  
Vol 3 (5) ◽  
pp. 374-381 ◽  
Author(s):  
A Verderber ◽  
KJ Gallagher
Keyword(s):  
Oxygen Consumption ◽  
Range Of Motion ◽  
Appreciable Effect ◽  
Convenience Sample ◽  
Men And Women ◽  
Healthy Men ◽  
Significant Difference ◽  
The Mean ◽  
Oxygen Requirements ◽  
Back Care

BACKGROUND: Baseline data are needed to provide a foundation for future studies investigating the effects of various nursing interventions on the oxygen requirements of critically ill patients. OBJECTIVES: To establish reference values for the oxygen requirements of adults in response to three common patient events: a nurse-administered bed bath, passive range-of-motion exercises, and turning from side to side; and to determine whether the order in which the interventions were administered had an effect on oxygen consumption. METHODS: A convenience sample of 30 healthy men and women were randomly assigned to one of three treatment groups, for which the order in which interventions were to be administered had previously been designated. Data were analyzed using analysis of variance with repeated measures. RESULTS: There was a significant difference in the mean oxygen consumption among activities, as well as between men and women, with men having a significantly higher mean oxygen consumption than that of the women. For both men and women, the mean oxygen consumption during unassisted turning and back care was significantly higher than at baseline. The oxygen consumption for men averaged 4.25 mL/kg per minute, SD = .71 at baseline, 5.08 mL/kg per minute, SD = .98 for turning, and 4.72 mL/kg per minute, SD = .90 during back care. Women averaged 3.74 mL/kg per minute, SD = .49 at baseline, 4.48 mL/kg per minute, SD = .85 for turning, and 3.89 mL/kg per minute, SD = 1.15 during back care. Changes in oxygen consumption for other interventions were nonsignificant and negligible. Oxygen consumption returned to near baseline within 15 minutes of cessation of activity. The order in which interventions were administered did not have a significant effect on oxygen consumption. CONCLUSIONS: The anterior bath and passive range of motion exercises have minimal effect on oxygen consumption. Turning and back care significantly increase oxygen consumption from the baseline value. The order in which interventions are administered does not have any appreciable effect on oxygen consumption.

scholarly journals Impact of Iron Status on Thyroid Function: A Community Based Cross-Sectional Study in Eastern Nepal

2019 ◽  
Vol 15 (1) ◽  
pp. 18-21 ◽  
Author(s):  
Saroj Thapa ◽  
Madhab Lamsal ◽  
Sanjay Kumar Sah ◽  
Rajendra Kumar Chaudhari ◽  
Basanta Gelal ◽  
...  
Keyword(s):  
Iron Deficiency ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Community Based ◽  
Cross Sectional ◽  
Hormone Profile ◽  
Iron Deficient ◽  
The Mean

Background: Iron deficiency is the most common nutritional deficiency in the world. The relation between thyroid hormones and iron status is bidirectional. The aim of this study was to assess iron nutrition status and evaluate its relationship with thyroid hormone profile among children of Eastern Nepal. Methods: A  community based cross-sectional study was conducted in eastern Nepal. A total of 200 school children aged 6-12 years were recruited after taking informed consent from their guardians. Blood samples were collected and assayed for free thyroid hormones (fT3 and fT4), thyroid stimulating hormone (TSH), serum iron, total iron binding capacity (TIBC) concentration and percentage transferrin saturation was calculated. Results: The mean serum iron and TIBC was 74.04 µg/dl and 389.38 µg/dl respectively. The median transferring saturation was 19.21%. The overall prevalence of iron deficiency (Transferrin saturation < 16%) was 34% (n=68). The mean concentration of fT3 and fT4 was 2.87 pg/ml and 1.21 ng/dl respectively, while the median TSH concentration was 3.03 mIU/L. Median TSH concentration in iron deficient group (3.11 µg/dl) and iron sufficient group (2.91 µg/dl) was not significantly different. Among iron deficient children 5.9% had   subclinical hypothyroidism (n=4). Iron status indicators were not significantly correlated with thyroid profile parameters in the study population. Conclusions: The prevalence of iron deficiency is high and iron   deficiency does not significantly alter the thyroid hormone profile in the study region.

Growth Differentiation Factor 15 (GDF15) and Erythropoietin (EPO) Levels in Beta Talassemia Major Patients.

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 1881-1881
Author(s):  
Ilaria Salussoglia ◽  
Gisella Volpe ◽  
Silvia Fracchia ◽  
Simona Roggero ◽  
Filomena Longo ◽  
...  
Keyword(s):  
Iron Status ◽  
Clinical Status ◽  
Transferrin Saturation ◽  
Iron Chelation ◽  
Serum Levels ◽  
Thalassemia Major ◽  
Beta Thalassemia ◽  
Transfusion Therapy ◽  
Beta Thalassemia Major ◽  
The Mean

Abstract Background: The serum level of GDF15 has been recently indicated as a possible marker of erythropoiesis (Tanno et al., Nature 2007) suggesting a role of its over-expression in contributing to iron overload in thalassemia syndromes by inhibiting hepcidin expression. The aim of present study has been to evaluate GDF15 serum levels in a homogeneous series of thalassemia patients and the relationship with transfusional parameters and iron status markers. Methods: A group of consecutive patients with beta thalassemia major followed at our institution were included in the study. All patients were on regular transfusion and iron chelation treatment. Quantification of GDF15 on serum samples was performed with DuoSet ELISA for human GDF15 (R&D Systems) following the manufacturer’s protocol (Tanno et al., Nature 2007). Each patient had also a blood test for haemoglobin (Hb), serum iron, ferritin, transferrin, transferrin saturation and EPO levels. Liver Iron Concentration by SQUID and cardiac iron by MRI T2* have been assessed. The mean hemoglobin levels of the previous year (pre-transfusional, post-transfusional and mean) have been calculated for each individual. The presence of mild thalassemic mutations was used to classify mild or severe genotype. Clinical status has been assessed on the presence/absence of main complications (heart disease, liver disease, diabetes, hypothyroidism). Statistical analysis was performed using the software Statistica (StatSoft). Results: One hundred-forty patients (73 male, 67 females) were studied. The mean age was 27.9 ± 9.0 years (range: 3.5–42). One hundred (71%) were splenectomised. Betathalassemia major patients had elevated GDF15 serum levels (mean 6892 ± 6894 pg/mL; range 720–52521) in comparison with healthy volunteers (273 ± 104 pg/mL; range 129–401). GDF 15 levels were strongly related to EPO levels (r=0,81; p&lt;0,001). GDF15 levels were not related with age, gender, spleen, clinical status and iron markers. Patients with a severe genotype had higher GDF15 levels than mild genotype patients. GDF15 levels had a negative correlation with Hbs (p&lt;0,05 for actual Hb and pre-transfusional Hb; p&lt;0,001 for post-transfusional Hb and mean Hb). In thalassemia major patients with a severe genotype, GDF15 levels within thrice the normal range have been observed only in patients with pre-transfusional Hb above 9,6, post-transfusional Hb above 12,5 and a mean Hb above 11,3. Conclusions: In beta thalassemia major patients on regular transfusion and iron chelation, serum GDF15 levels are high, inversely related to the haemoglobin levels maintained. Further studies of this marker may lead to a rethinking of the optimal transfusion therapy in these conditions.

Role of Hepcidin as a biomarker for iron status in patients on regular hemodialysis after treatment of hepatitis C virus

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Magdy M El Sharkawy ◽  
Haitham E Abdelaziz ◽  
Lina E Khedr ◽  
Mohamed A Sharaf
Keyword(s):  
Hepatitis C ◽  
Iron Homeostasis ◽  
Complete Blood Count ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Renal Elimination ◽  
Serum Hepcidin ◽  
The Mean ◽  
Esrd Patients ◽  
Regular Hemodialysis

Abstract Background Hepcidin, an acute phase reactant protein produced in the liver, is a key regulator of iron homeostasis. Because of its renal elimination and regulation by inflammation, it is possible that progressive renal insufficiency leads to altered hepcidin metabolism. Aim of the work to assess effect of HCV treatment on hepcidin levels in regular heamodialysis patients and its relation to iron status. Patients and Methods This cross sectional study was conducted on 45 ESRD patients on regular hemodialysis. All candidates included in this study subjected to careful history taking, full clinical examination and investigations (including complete blood count, HCVAb, HBVsAg and HIVAb). All patients had positive antibodies for hepatitis C; however, all of them had negative PCR and negative HIV, HBV antibodies. Results In our study, dry weight ranged from 46 to 117 kg with mean 77.36. Weight gain ranged from 2 to 5 kg with mean 3.09.About 87% of patients had LTAVF access. About 12 patients (27%) received blood transfusion once. Timing of transfusion ranged from 4 to 60 month with median of 24 months. Regarding frequency of epoetin dose, 11 patients (24.4%) did not receive epoetin, 16 patients (35.6%) received it twice/week. Only 3 patients (6.7%) received iron therapy for time ranging from 3 to 5 months with mean of 4 months. Mean Hb concentration in our study population was 10.11 ±1.64 gm/dl. The mean of iron was 64.22 ±19.52, TIBC was 409.96 ± 67.85, ferritin was 394.56 ± 239.22 and TSAT% was 22.6 ±7.36. The mean serum hepcidin was 218.51 ±127. Our study demonstrated that increase in serum hepcidin is associated with lower serum Hb and iron levels. On the other hand, there is statistically significant positive correlation between serum hepcidin and both serum ferritin and transferrin saturation. Conclusion Median hepcidin value is elevated in dialysis ESRD patients due to increased inflammation and decreased clearance of hepcidin. Also serum hepcidin levels were lowered in HCV patients. Therefore ESRD patients on maintenance HD after treatment of HCV infection showed elevated levels of serum hepcidin.

scholarly journals Serum transferrin receptors are decreased in the presence of iron overload

1998 ◽  
Vol 44 (1) ◽  
pp. 40-44 ◽  
Author(s):  
Hlosukwazi Khumalo ◽  
Zvenyika A R Gomo ◽  
Victor M Moyo ◽  
Victor R Gordeuk ◽  
Thokozile Saungweme ◽  
...  
Keyword(s):  
Iron Overload ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Cellular Level ◽  
Receptor Expression ◽  
Serum Transferrin ◽  
Transferrin Receptors ◽  
Indirect Measures ◽  
The Mean ◽  
Transferrin Receptor Expression

Abstract To test the hypothesis that the quantities of circulating transferrin receptors are reduced in iron overload, we studied serum transferrin receptors and indirect measures of iron status in 150 subjects from rural Zimbabwe. We found significant inverse correlations between serum concentrations of transferrin receptors and ferritin, the ratio of ferritin to aspartate aminotransferase, and transferrin saturation (r ≥0.44; P &lt;0.001). The mean ± SD concentration of serum transferrin receptors in 23 subjects classified as having iron overload (ferritin &gt;300 μg/L and transferrin saturation &gt;60%) was 1.55 ± 0.61 mg/L, significantly lower than the 2.50 ± 0.62 mg/L in 75 subjects with normal iron stores (ferritin 20–300 μg/L and transferrin saturation 15–55%; P &lt;0.0005) and the 2.83 ± 1.14 mg/L in 8 subjects with iron deficiency (ferritin &lt;20 μg/L; P = 0.001). In keeping with the regulation of transferrin receptor expression at the cellular level, our findings suggest that serum transferrin receptors are decreased in the presence of iron overload.

scholarly journals Serum Hepcidin Levels and Reticulocyte Hemoglobin Concentrations as Indicators of the Iron Status of Peritoneal Dialysis Patients

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Aya Eguchi ◽  
Takahiro Mochizuki ◽  
Misao Tsukada ◽  
Koji Kataoka ◽  
Yukio Hamaguchi ◽  
...  
Keyword(s):  
Peritoneal Dialysis ◽  
Iron Status ◽  
Transferrin Saturation ◽  
Serum Levels ◽  
Dialysis Patients ◽  
Reliable Measurement ◽  
Hepcidin Expression ◽  
Serum Hepcidin ◽  
Iron Parameters ◽  
The Mean

Hepcidin is the key mediator of renal anemia, and reliable measurement of serum hepcidin levels has been made possible by the ProteinChip system. We therefore investigated the iron status and serum hepcidin levels of peritoneal dialysis (PD) patients who had not received frequent doses of an erythrocytosis-stimulating agent (ESA) and had not received iron therapy. In addition to the usual iron parameters, the iron status of erythrocytes can be determined by measuring reticulocyte hemoglobin (RET-He). The mean serum hepcidin level of the PD patients(n=52)was 80.7 ng/mL. Their serum hepcidin levels were significantly positively correlated with their serum ferritin levels and transferrin saturation (TSAT) levels, but no correlations were found between their serum hepcidin levels and RET-He levels, thereby suggesting that hepcidin has no effect on the iron dynamics of reticulocytes. Since low serum levels of CRP and IL-6, biomarkers of inflammation, were not correlated with the serum hepcidin levels, there is likely to be a threshold for induction of hepcidin expression by inflammation.

scholarly journals The effect of low doses of betaine on plasma homocysteine in healthy volunteers

2004 ◽  
Vol 92 (4) ◽  
pp. 665-669 ◽  
Author(s):  
Georg Alfthan ◽  
Kaisa Tapani ◽  
Katja Nissinen ◽  
Janna Saarela ◽  
Antti Aro
Keyword(s):  
Folic Acid ◽  
Vascular Diseases ◽  
Dependent Manner ◽  
Men And Women ◽  
Healthy Men ◽  
Plasma Thcy ◽  
Total Homocysteine ◽  
The Mean ◽  
Betaine Homocysteine Methyltransferase ◽  
Dose Dependent

Homocysteine is a risk factor for vascular diseases, and lowering of plasma total homocysteine (tHcy) may be beneficial for health. Homocysteine can be remethylated to methionine by betaine–homocysteine methyltransferase using betaine (2(N,N,N-trimethyl)glycine) as methyl donor. A dose of 6 g betaine/d has been used in the treatment of homocystinuria, but data on the dose–response are scarce. Thirty-four healthy men and women were supplied with doses of 1, 3 and 6 g betaine and then with 6 g betaine + 1 mg folic acid for four consecutive 1-week periods. The mean plasma tHcy concentration decreased by 1·1 (NS), 10·0 and 14·0 % (P<0·001) after supplementation with 1, 3 and 6 g betaine respectively. A further decrease in plasma tHcy by 5 % (P<0·01) was achieved by combining 1 mg folic acid with the 6 g betaine dose. Plasma betaine increased from 31 (SD 13) to 255 (SD 136) μmol/l in a dose-dependent manner (R20·97). We conclude that plasma tHcy is lowered rapidly and significantly by 3 or 6 g betaine/d in healthy men and women.

Resistance Training Affects Iron Status in Older Men and Women

2001 ◽  
Vol 11 (3) ◽  
pp. 287-298 ◽  
Author(s):  
Laura E. Murray-Kolb ◽  
John L. Beard ◽  
Lyndon J. Joseph ◽  
Stephanie L. Davey ◽  
William J. Evans ◽  
...  
Keyword(s):  
Resistance Training ◽  
Binding Capacity ◽  
Iron Status ◽  
Hematological Parameters ◽  
Transferrin Saturation ◽  
Older Men ◽  
Resistance Training Program ◽  
Men And Women ◽  
Before And After ◽  
Older Men And Women

Objective:To examine the effects of resistance training on hematological and selected indices of iron status in 17 women aged 54–71 years and 18 men aged 56–69 years.Design:Tests and evaluations were done before and after all subjects participated in a resistance training program twice weekly for 12 weeks.Results:The resistance training was effective as evidenced by increases in skeletal muscle strength of 20 ± 9% and 23 ± 13% for the men and women, respectively. Hematological parameters and serum iron concentrations were within normal clinical ranges and were unchanged by resistance training for both the men and the women. Total iron binding capacity (TIBC) and transferrin saturation were also unaffected by resistance training in the women but were significantly affected in the men. The men showed a decreased TIBC (p < .0001) and an increased transferrin saturation (p = .050). Serum ferritin concentrations decreased significantly in the women (p = .041) but were unchanged in the men. Transferrin receptor concentrations were unaffected by resistance training in the women but increased significantly in the men (p = .030).Conclusions:With resistance training, iron status of older men and women changes in a sex specific way.

Sign in / Sign up

Export Citation Format

Share Document