Unreliability of immunochemical determination of lactate dehydrogenase isoenzyme-1 in heparinized plasma.

1984 ◽  
Vol 30 (11) ◽  
pp. 1807-1808 ◽  
Author(s):  
G Lum
Keyword(s):  
Lactate Dehydrogenase ◽  
Mean Difference ◽  
Normal Group ◽  
Plasma Samples ◽  
Group Iii ◽  
Dehydrogenase Isoenzyme ◽  
Group I ◽  
Immunochemical Determination ◽  
Paired Serum

Abstract Paired serum and heparinized plasma samples were assayed simultaneously for lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 (LD1) activity by a commercially available immunochemical procedure. For all sera specimens tested, only LD1 activity was detected. For heparinized plasma, random discrepancies in LD1 activity were noted at normal (Group I), borderline (Group II), and increased (Group III) total LD activity. Incomplete precipitation of LD-M subunits was confirmed by electrophoresis and occurred in eight of 15, four of 13, and eight of 22 instances (total: 20/50, or 40%) with a mean difference of 13, 10.6, and 18.2 U/L (8.3, 4.0, and 4.4%) in Groups I, II, and III, respectively. We conclude that heparinized plasma is an unsuitable sample for the immunochemical determination of LD1.

Clinical evaluation of immunochemical assay of lactate dehydrogenase isoenzyme 1 in early detection of acute myocardial infarction.

1982 ◽  
Vol 28 (10) ◽  
pp. 2152-2154 ◽  
Author(s):  
T Y Wang ◽  
J H Godfrey ◽  
L G Graham ◽  
M N Haddad ◽  
T C Hamilton
Keyword(s):  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Lactate Dehydrogenase ◽  
Myocardial Ischemia ◽  
Clinical Presentation ◽  
Myocardial Infarct ◽  
Acute Myocardial Infarct ◽  
Group Iii ◽  
Dehydrogenase Isoenzyme ◽  
Group I

Abstract We immunochemically measured lactate dehydrogenase isoenzyme 1 (LD-1), calculated LD-1/LD ratios (% LD-1) for 122 specimens from 60 patients, and compared the results with those for the conventional cardiac profile and other findings such as clinical presentation and electrocardiogram. Results for LD-1 and % LD-1 could be classified into three groups: group I, with LD-1 less than 64 U/L; group II, with LD-1 greater than 64 U/L and % LD-1 between 17 and 37%; and group III, with LD-1 greater than 64 U/L and 5 LD-1 greater than 38%. These three groups correlated closely and consistently with three patients of cardiac profile, i.e., those of no acute myocardial infarct, myocardial ischemia, and acute myocardial infarct, respectively.

scholarly journals Concentration of hepatic vitamins A and E in rats exposed to chlorpyrifos and/or enrofloxacin

2016 ◽  
Vol 19 (2) ◽  
pp. 371-378 ◽  
Author(s):  
A. Spodniewska ◽  
D. Barski
Keyword(s):  
Lipid Peroxidation ◽  
Free Radicals ◽  
Gastric Tube ◽  
Initial Period ◽  
Hplc Method ◽  
Antioxidant Vitamin ◽  
Exposure Group ◽  
Group Iii ◽  
Group I

Abstract The aim of the study was to determine the level of antioxidant vitamins A and E in the liver of rats exposed to chlorpyrifos and/or enrofloxacin. Chlorpyrifos (Group I) was administered at a dose of 0.04 LD50 (6 mg/kg b.w.) for 28 days, and enrofloxacin (Group II) at a dose of 5 mg/kg b.w. for 5 consecutive days. The animals of group III were given both of the mentioned above compounds at the same manner as groups I and II, but enrofloxacin was applied to rats for the last 5 days of chlorpyrifos exposure (i.e. on day 24, 25, 26, 27 and 28). Chlorpyrifos and enrofloxacin were administered to rats intragastrically via a gastric tube. The quantitative determination of vitamins was made by the HPLC method. The results of this study indicated a reduction in the hepatic concentrations of vitamins A and E, compared to the control, which sustained for the entire period of the experiment. The four-week administration of chlorpyrifos to rats resulted in a significant decrease of vitamins in the initial period of the experiment, i.e. up to 24 hours after exposure. For vitamin A the maximum drop was observed after 24 hours (19.24%) and for vitamin E after 6 hours (23.19%). Enrofloxacin caused a slight (3-9%) reduction in the level of the analysed vitamins. In the chlorpyrifos-enrofloxacin co-exposure group reduced vitamins A and E levels were also noted, but changes in this group were less pronounced in comparison to the animals intoxicated with chlorpyrifos only. The decrease in the antioxidant vitamin levels, particularly noticeable in the chlorpyrifos- and the chlorpyrifos combined with enrofloxacin-treated groups, may result not only from the increase in the concentration of free radicals, but also from the intensification of the secondary stages of lipid peroxidation.

Pyruvate as Substrate in the Determination of Serum Lactate Dehydrogenase Isoenzyme Activity

1974 ◽  
Vol 20 (11) ◽  
pp. 1462-1465 ◽  
Author(s):  
Doris McKenzie ◽  
A R Henderson
Keyword(s):  
Lactate Dehydrogenase ◽  
Serum Lactate ◽  
Serum Lactate Dehydrogenase ◽  
Dehydrogenase Isoenzyme

Abstract Lactate dehydrogenase isoenzyme activity is usually assessed, after the isoenzymes have been separated, by reactions involving lactate as the substrate. We describe a method for their assessment with pyruvate as the substrate. Precision is adequate as compared to the conventional methodology. Application of this type of approach (measurement of a decrease in fluorescence) to determination of other serum isoenzymes is briefly described.

Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum: in vivo and in vitro studies

1990 ◽  
Vol 36 (9) ◽  
pp. 1638-1641 ◽  
Author(s):  
S C Kazmierczak ◽  
W J Castellani ◽  
F Van Lente ◽  
E D Hodges ◽  
B Udis
Keyword(s):  
Flow Cytometry ◽  
Lactate Dehydrogenase ◽  
Cell Types ◽  
In Vitro Studies ◽  
Hemolytic Disease ◽  
Dehydrogenase Isoenzyme ◽  
Disease Analysis

Abstract We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD; EC 1.1.1.27) isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenzyme distribution between these two cell types. The ability of hemolysis to cause a "flipped" LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.

scholarly journals Effects of Enoant and Ischemia and Reperfusion on Lens Metabolites of Rats

2013 ◽  
Vol 2013 ◽  
pp. 1-7
Author(s):  
Hülya Güngel ◽  
Asiye Nurten ◽  
İhsan Kara ◽  
Serife Evrim Kepekci Tekkeli ◽  
Elif Özkök ◽  
...  
Keyword(s):  
Wistar Rats ◽  
The Other ◽  
Ischemia And Reperfusion ◽  
Polyphenol Content ◽  
Membrane Stabilization ◽  
Group Iii ◽  
Group I ◽  
Ischemia Group ◽  
Dry Diet

The effects of the ischemia and reperfusion on the lens metabolites and the effect of a phytotherapeutic commercial product called “Enoant” (mixed polyphenol content) on the selected lens metabolites were investigated. For this aim, 30 Wistar rats were divided into three groups according to their diet and being subjected to ischemia. 10 of the rats as Group I were fed on dry diet; the other 10 (Group II) were fed on dry diet and drinking water with Enoant. At the end of 15 days period, both groups of rats were subjected to ischemia for 2 hours and reperfused. After another 15 days with their same diet, the rats were decapitated. The remaining 10 rats, which were not subjected to ischemia (Group III), were fed on dry diet only. 1HNMR spectroscopy was used for the determination of lens metabolites of each group of rats. The results obtained from the three groups have been compared statistically. The differences of metabolites were significant except pyruvate and succinate. Oral administration of Enoant revealed effects on increasing membrane stabilization, the antioxidant capacity, osmotic regulator molecule capacity, and sorbitol content of lens disturbed by ischemia. Enoant can be used where oxidative or osmotic stress is formed.

scholarly journals Determination of thiol/disulphide homeostasis as a new indicator of oxidative stress in dairy cows with subclinical endometritis

2021 ◽  
Vol 91 (2) ◽  
pp. 137-148
Author(s):  
Birten Emre ◽  
◽  
Ömer Korkmaz ◽  
Ismail Koyuncu ◽  
Selim Çomaklı ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dairy Cows ◽  
Immunocytochemical Staining ◽  
Control Group ◽  
Roc Curve Analysis ◽  
Group Iii ◽  
Total Thiol ◽  
Group I ◽  
Subclinical Endometritis

The objective of this study was to determine thiol/disulphide homeostasis (TDH) in infertile cows with subclinical endometritis (SCE). Endometrial cytological samples were collected using a cytobrush to diagnose SCE in 36 infertile cows. According to the results of the cytology examination, those with acute endometritis were classified as Group I (n = 20) and those with chronic endometritis were classified as Group II (n = 16). A control group was formed of heifers as Group III (n = 20). Blood samples were taken from each group on the day of diagnosis (day 0) to analyse TDH. In the cytology examination, both the Giemsa method and immunocytochemical staining were applied to determine chronic inflammation and activity status. In 55.55% (20/36) of the infertile cows with cytological endometritis, the inflammation was determined to be active, and in 44.44% (16/36) it had become chronic. The native thiol and total thiol levels were found to be statistically significantly lower in the acute (206.54 ± 8.30 μmol/L; 227.11 ± 9.30 μmol/L) and chronic SCE cases (225.15 ± 11.89 μmol/L; 247.96 ± 10.80 μmol/L) compared to the heathy control group (308.47 ± 13.59 μmol/L; 336.83 ± 15.5 μmol/L respectively) (P<0.001). Disulphide levels, disulphide/total thiol, native thiol/total thiol and disulphide/native thiol ratios were similar in all the groups (P>0.05). The diagnostic accuracy of native thiol, which can be used in the diagnosis of SCE, was 92.8%, that of total thiol was 89.3% and that of disulphide was 64.3% according to the ROC curve analysis. These results demonstrate that TDH is a reliable and sensitive indicator of oxidative stress in cow SCE, and that abnormal TDH might play a role in SCE pathogenic mechanisms. This is the first study to evaluate thiol/disulphide homeostasis in dairy cows with SCE as a new indicator of oxidative stress.

scholarly journals Problem of Ametropia in Children in Russian Federation

2018 ◽  
Vol 15 (2S) ◽  
pp. 40-44
Author(s):  
El Yu. Markova ◽  
L. Yu. Bezmel’nitsyna ◽  
O. V. Kurganova ◽  
N. A. Pron’ko ◽  
L. V. Venediktova
Keyword(s):  
Electrophysiological Study ◽  
Economic Assessment ◽  
Medical Costs ◽  
Refractive Errors ◽  
Mathematical Methods ◽  
Group Iii ◽  
Social Burden ◽  
Group I ◽  
High Level

Refractive errors are remaining one of the most important problems in pediatric ophthalmology. At present, there are changes in organization approaches of prophylactic medical examinations in children. It causes the necessary of comprehensive clinical and economic assessment of their effectiveness.Purpose: to develop the recommendations for improvement of ophthalmological care for children with refractive errors. For diagnostics we used visometry, determination the vision characteristics, determination of chromosensitivity, biomicroscopy, definition of refraction of manifest and cycloplegic, determination of volume and accommodation reserves, ophthalmoscopy, electrophysiological study (EFI), optical coherence tomography (OCT), fundusography.Methods. A retrospective analysis of typical practice of treatment patients with amblyopia and strabismus was organized. Data was processed with mathematical methods and descriptive statistics. Modeling, Budget impact and burden of diseases analyses were used for clinicoeconomical evaluation.Results. 636 patients aged from 5 to 7 years old were included. Depending on the age of the diagnosis of ametropia, three groups were identified. Group I (318 patients): the diagnosis was detected up to 1 year, group II (190 patients) — at the age from 1 to 3 years, Group III: (127 patients) from 3 to 7 years. A direct correlation between the age of ametropia diagnostics and frequency such complications as amblyopia, disturbance of binocular vision and strabismus development of strabismus was established. According to the results of clinical and economic analysis, uncorrected refractive errors in children are characterized by a high level of economic and social burden of disease (the older the age of ametropia diagnosis caused increase of direct medical and non-medical costs). With regard to non-medical costs, we established that a reduction the frequency of courses in vision office and operational treatment results in a reduction in payments to parents on temporary incapacity sheets.Conclusion: the optimal age for diagnostics of refractive errors in up to 1year from both clinical and economic perspective. 

Evaluation of determination of lactate dehydrogenase isoenzyme 1 by chemical inhibition with perchlorate or with 1,6-hexanediol

1990 ◽  
Vol 36 (2) ◽  
pp. 355-358 ◽  
Author(s):  
J M Paz ◽  
A Garcia ◽  
M Gonzales ◽  
M Treviño ◽  
J C Tutor ◽  
...  
Keyword(s):  
Lactate Dehydrogenase ◽  
Coefficient Of Variation ◽  
Low Cost ◽  
The Other ◽  
Pairwise Correlation ◽  
Dehydrogenase Isoenzyme ◽  
Acceptable Limit ◽  
Chemical Inhibition

Abstract We have evaluated the determination of lactate dehydrogenase (EC 1.1.1.27) isoenzyme 1 activity by chemical inhibition of the other isoenzymes with perchlorate and with 1,6-hexanediol. In the hexanediol method, we studied the effect of the duration of incubation with the inhibitor; a 5-min incubation yielded results closest to those of an immunochemical technique (Isomune-LD). The perchlorate method was the most precise, and the hexanediol method the least, although for none of the techniques did the coefficient of variation exceed the medically acceptable limit prescribed by the College of American Pathologists. Pairwise correlation among the immunoprecipitation, electrophoretic, and chemical inhibition methods was good (r greater than 0.991), although the differences between means were statistically significant (except for the comparison of the two chemical inhibition methods). Because of its ease, low cost, and precision, we recommend the perchlorate method for routine use.

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