More-sensitive enzyme-multiplied immunoassay technique for procainamide and N-acetylprocainamide in plasma, serum, and urine.

1988 ◽  
Vol 34 (5) ◽  
pp. 957-960 ◽  
Author(s):  
P R Henry ◽  
R A Dhruv
Keyword(s):  
Quantitative Analysis ◽  
Plasma Concentration ◽  
Controlled Release ◽  
Quantitative Determination ◽  
Dosage Forms ◽  
Clinical Samples ◽  
Immunoassay Technique ◽  
Analytical Recovery ◽  
Serum And Urine

Abstract A commercially available (Syva Co.) enzyme-multiplied immunoassay technique (EMIT) for the quantitative determination of procainamide (PA) and N-acetylprocainamide (NAPA) was modified to allow automated quantitative analysis of approximately 100 samples per day, in a working range of 0.1 to 2.0 micrograms/mL. Such a test was needed to evaluate the pharmacokinetic characteristics of controlled-release dosage forms characterized by long half-lives at low plasma concentration. Analytical recovery of PA and NAPA from serum, plasma, and urine was satisfactory, but at extreme ratios for PA:NAPA the accuracy of determining the lower-concentration component became unsatisfactory. In fact, however, we found no such ratios in 5400 clinical samples assayed by this procedure.

scholarly journals QUANTIFICATION OF ANTICOAGULANTS DABIGATRAN, RIVAROXABAN, AND PRASUGREL BY CHROMATOGRAPHIC AND SPECTROMETRIC TECHNIQUES – REVIEW

2019 ◽  
pp. 1-8
Author(s):  
SATYA PRASAD B ◽  
JAYAKUMARI S
Keyword(s):  
Quantitative Analysis ◽  
Quantitative Determination ◽  
Analytical Techniques ◽  
Dosage Forms ◽  
Clinical Isolates ◽  
Advantages And Disadvantages

A review is presented on different analytical techniques used for quantitative analysis of selected anticoagulants dabigatran, rivaroxaban, and prasugrel. Efforts have been made to collate all the relevant references to the extent possible. The review discusses the advantages and disadvantages of the cited analytical techniques, which will help to give insights into the methods used for estimation of selected anticoagulants such as dabigatran, rivaroxaban, and prasugrel, from clinical isolates, and its dosage forms. The review highlights the basic as well as advanced techniques performed for estimating dabigatran, rivaroxaban, and prasugrel. The techniques illustrated here have been demonstrated to be useful for quantitative determination of selected anticoagulants and may find application in analyzing other related properties.

Qualitative and quantitative determination of trace aldehydes and ketones in food preservative propionic acid for quality improvement

2021 ◽  
Author(s):  
Yiwen Cao ◽  
Shenjie Zhu ◽  
Lin Zhang ◽  
Qun Cui ◽  
Haiyan Wang
Keyword(s):  
Quality Improvement ◽  
Quantitative Analysis ◽  
Quantitative Determination ◽  
Propionic Acid ◽  
Qualitative And Quantitative Analysis ◽  
Food Preservative ◽  
Qualitative And Quantitative ◽  
Aldehydes And Ketones ◽  
Aldehyde Content

Aiming at the difficulty in qualitative and quantitative analysis of trace compositions in food preservative propionic acid, the trace compositions and the key components influencing the total aldehyde content in...

Quantitative determination of tolazoline in serum and urine

1985 ◽  
Vol 338 ◽  
pp. 123-130 ◽  
Author(s):  
Michael J. Cwik ◽  
Gregory P. Chiu ◽  
James H. Fischer ◽  
Elizabeth Chow-Tung ◽  
Bruce L. Currie
Keyword(s):  
Quantitative Determination ◽  
Serum And Urine

Quantification Of Novel Dpp4 Inhibitor - Vildagliptin By Spectrophotometric And Chromatographic Techniques: Brief Review

2021 ◽  
pp. 5603-5610
Author(s):  
Gnanasekaran D. ◽  
Gandhimathi R.
Keyword(s):  
Quantitative Analysis ◽  
Analytical Techniques ◽  
Dosage Forms ◽  
Major Focus ◽  
Pharmaceutical Dosage Forms ◽  
Dipeptidyl Peptidase 4 ◽  
Chromatographic Techniques ◽  
Dipeptidyl Peptidase 4 Inhibitor ◽  
Pros And Cons

A review is presented on different analytical techniques used for quantitative analysis of novel Dipeptidyl peptidase-4 inhibitor (DPP-4) - Vildagliptin. Endeavours have been made to examine all the pertinent references to the degree conceivable. The review discusses the pros and cons of the cited analytical techniques, which will aid to give understand into the methods used for determination of Vildagliptin, from clinical isolates and from its pharmaceutical dosage forms. The major focus of this review is the basic as well as advanced analytical techniques established for determination of Vildagliptin. The procedures outlined here have been exhibited to be helpful for assessment of Vildagliptin and may discover application in dissecting other related properties.

Determination of creatinine in serum and urine by a rapid liquid-chromatographic method

1990 ◽  
Vol 36 (6) ◽  
pp. 830-836 ◽  
Author(s):  
R Paroni ◽  
C Arcelloni ◽  
I Fermo ◽  
P A Bonini
Keyword(s):  
Signal To Noise Ratio ◽  
Liquid Chromatographic Method ◽  
Analytical Recovery ◽  
Internal Standardization ◽  
Assay Precision ◽  
Fuller’S Earth ◽  
Liquid Chromatographic ◽  
Specific Evaluation ◽  
Serum And Urine

Abstract We describe an HPLC ion-pair procedure for rapid and specific evaluation of creatinine in serum and urine. We used a 15 cm X 4.6 mm ODS column with a 50/50 (by vol) mixture of sodium decanesulfonic acid (10 mmol/L, pH 3.2) and methanol and measured absorbance at 236 nm. Serum (100 microL) or 30-fold-diluted urine (100 microL) was added to 400 microL of acetone. After centrifugation, the supernates (300 microL) were dried, reconstituted with the mobile phase, and injected into the HPLC. Assay precision was tested for concentrations of 10, 29, and 130 mg/L and yielded, respectively, 3.1%, 2.1%, and 1.1% for within-day CV and 2.8%, 2.1%, and 2.2% for total CV. Analytical recovery was 102 (+/- 6.7%). Linearity was demonstrated in the 0-200 mg/L range for serum and 0-3.5 g/L range for urine (r greater than or equal to 0.999). The detection limit for creatinine (signal-to-noise ratio = 3) was 0.5 mg/L. We used cimetidine for internal standardization. Correlation was good between this procedure and the Jaffé kinetic, the enzymatic (creatinine amidohydrolase), and the Fuller's earth alkaline picrate methods.

GLC Determination of the Optical Isomers of Amphetamine

1970 ◽  
Vol 53 (1) ◽  
pp. 113-115
Author(s):  
Clyde E Wells
Keyword(s):  
Quantitative Determination ◽  
Calibration Curve ◽  
Collaborative Study ◽  
Isomeric Ratio ◽  
Dosage Forms ◽  
Optical Isomers ◽  
Standard Calibration ◽  
Tablet Dosage

Abstract A method is described for the quantitative determination of the ratio of d- and l-amphetamine stereoisomers by GLC. A derivative formed with N-trifluoroacetyl-(l)-prolyl chloride is chromatographed and the isomeric ratio is read from a standard calibration curve. The method is applicable to crystalline salts of amphetamine and to commercial tablet dosage forms. It is recommended that this method be subjected to collaborative study.

Modification of the choriogonadotropin beta-subunit radioimmunoassay for determination of urinary choriogonadotropin.

1978 ◽  
Vol 24 (11) ◽  
pp. 1958-1961 ◽  
Author(s):  
J McCready ◽  
G D Braunstein ◽  
D Helm ◽  
M E Wade
Keyword(s):  
Pregnant Women ◽  
Radioreceptor Assay ◽  
Beta Subunit ◽  
Urine Samples ◽  
Human Choriogonadotropin ◽  
Analytical Recovery ◽  
Antibody Method ◽  
Serum And Urine ◽  
Serum And Urine Samples

Abstract The choriogonadotropin beta-subunit radioimminoassay has been used extensively to measure human choriogonadotropin in the sera of pregnant women and individuals with trophoblastic and nontrophoblastic tumors. Unmodified, this method cannot be used to measure choriogonadotropin in urine because of interfering substances. We circumvented the non-parallelism between the standards and serial dilutions of urine containing choriogonadotropin by adding pooled urine from men to the standard tubes and limiting the volume of urine to 100 microliter. This modified assay has a sensitivity of 3 int. units/liter of urine and is specific for choriogonadotropin concentrations of 40 int. units/liter of urine. Analytical recovery of choriogonadotropin added to urine ranged from 96 to 105%. The within-assay CV was 7.6%; the between-assay CV was 11.8%. Concentrations of choriogonadotropin in concurrently collected serum and urine samples from pregnant women correlated well. The test can be performed within 24 h by using the double-antibody method for separating bound from free hormone, or in 3 h with a dioxane method. The assay is about 20-fold more sensitive than the 2-min or 2-h slide and tube pregnancy tests, and seven-to 12-fold more sensitive than the radioreceptor assay.

Determination of Serum and Urinary Phenylalanine by Gas Chromatography

1973 ◽  
Vol 19 (5) ◽  
pp. 496-498 ◽  
Author(s):  
Z K Shihabi ◽  
G K Summer
Keyword(s):  
Gas Chromatography ◽  
Quantitative Analysis ◽  
Nitrous Acid ◽  
Gas Chromatograph ◽  
Phenyllactic Acid ◽  
Mild Conditions ◽  
Amino Acid Analyzer ◽  
Urine Specimens ◽  
Serum And Urine

Abstract Phenylalanine in serum and urine was determined by gas chromatography after it was converted, by action of nitrous acid, to the corresponding hydroxy acid, phenyllactic acid. After extraction with ether, the phenyllactic acid was derivatized with bis(trimethylsilyl)trifluoroacetamide under mild conditions, and the product was injected directly into the gas chromatograph. The precision of the method was verified by recovery studies and by comparison with the results of quantitative analysis of phenylalanine on an amino acid analyzer. Urine specimens from normal infants and children and from patients with phenylketonuria were analyzed by this method to show that the procedure is applicable to diagnosis of the disorder.

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