Determination of branched-chain amino acids and tyrosine in serum of patients with various hepatic diseases, and its clinical usefulness.

1989 ◽  
Vol 35 (7) ◽  
pp. 1399-1403 ◽  
Author(s):  
Y Azuma ◽  
M Maekawa ◽  
Y Kuwabara ◽  
T Nakajima ◽  
K Taniguchi ◽  
...  
Keyword(s):  
Amino Acids ◽  
Clinical Laboratory ◽  
Branched Chain Amino Acids ◽  
Isoleucine Leucine ◽  
Hepatic Diseases ◽  
Enzymatic Determination ◽  
Branched Chain ◽  
Routine Clinical Laboratory ◽  
Parenchymal Damage

Abstract We developed an automated enzymatic method for determination of the branched-chain amino acids (BCAAs; valine, isoleucine, leucine) and tyrosine in serum, and applied it to the clinical evaluation of patients with various hepatic diseases. Analytically, the test results were acceptably precise and reproducible, and correlated well with results obtained with an amino acid analyzer. Clinically, we found that a decrease in BCAAs, an increase in tyrosine, and the BCAAs/tyrosine ratio in serum paralleled the severity of hepatic parenchymal damage. We conclude that this enzymatic determination of BCAAs and tyrosine is simple and convenient enough for routine clinical laboratory use, and that the ratio of BCAAs/tyrosine obtained may be a good indicator of the severity of hepatic disorders.

scholarly journals Enzymic determination of branched-chain amino acids and 2-oxoacids in rat tissues. Transfer of 2-oxoacids from skeletal muscle to liver in vivo

1980 ◽  
Vol 188 (3) ◽  
pp. 705-713 ◽  
Author(s):  
G Livesey ◽  
P Lund
Keyword(s):  
Skeletal Muscle ◽  
Amino Acids ◽  
Bacillus Subtilis ◽  
Branched Chain Amino Acids ◽  
Rat Tissues ◽  
Arterial Blood ◽  
Branched Chain ◽  
Arteriovenous Difference

1. A procedure is described for the purification of leucine dehydrogenase (EC 1.4.1.9) from Bacillus subtilis. 2. The preparation is suitable for the quantitative assay of branched-chain amino acids and their 2-oxoacid analogues. 3. The content of total branched-chain 2-oxoacids in freeze-clamped liver, kidney, heart or mammary gland of fed rats is less than 5 nmol/g fresh wt. Higher amounts are present in skeletal muscle and arterial blood (25 +/- 4 nmol per g fresh wt., and 33 +/- 6 nmol per ml respectively; means +/- S.D. of 3 and 11 animals respectively). The values are not significantly affected by starvation for 24 h. 4. Arteriovenous difference measurements show that considerable amounts of branched-chain 2-oxoacids are released by skeletal muscle into the circulation and similar amounts are removed by the liver (about 1 mmol/24 h in a 400 g rat).

Bootstrapped neural network models for analyzing the responses of broiler chicks to dietary protein and branched chain amino acids

2014 ◽  
Vol 94 (1) ◽  
pp. 79-85 ◽  
Author(s):  
A. Faridi ◽  
A. Golian ◽  
A. Heravi Mousavi ◽  
J. France
Keyword(s):  
Neural Network ◽  
Amino Acids ◽  
Dietary Protein ◽  
Average Daily Gain ◽  
Network Models ◽  
Branched Chain Amino Acids ◽  
Broiler Chicks ◽  
Neural Network Models ◽  
Isoleucine Leucine ◽  
Branched Chain

Faridi, A., Golian, A., Heravi Mousavi, A. and France, J. 2014. Bootstrapped neural network models for analyzing the responses of broiler chicks to dietary protein and branched chain amino acids. Can. J. Anim. Sci. 94: 79–85. Reliable prediction of avian responses to dietary nutrients is essential for planning, management, and optimization activities in poultry nutrition. In this study, two bootstrapped neural network (BNN) models, each containing 100 separated neural networks (SNN), were developed for predicting average daily gain (ADG) and feed efficiency (FE) of broiler chicks in response to intake of protein and branched chain amino acids (BCAA) in the starter period. Using a re-sampling method, 100 different batches of data were generated for both the ADG and FE sets. Starting with 270 data lines extracted from eight studies in the literature, SNN models were trained, tested, and validated with 136, 67, and 67 data lines, respectively. All 200 SNN models developed, along with their respective BNN ones, were subjected to optimization (to find the optimum dietary protein and BCAA levels that maximize ADG and FE). Statistical analysis indicated that based on R 2, the BNN models were more accurate in 76 and 56 cases (out of 100) compared with the SNN models developed for ADG and FE, respectively. Optimization of the BNN models showed protein, isoleucine, leucine, and valine requirements for maximum ADG were 231.80, 9.05, 14.03 and 10.90 g kg−1 of diet, respectively. Also, maximum FE was obtained when the diet contained 232.30, 9.07, 14.50, and 11.04 g kg−1 of protein, isoleucine, leucine, and valine, respectively. The results of this study suggest that in meta-analytic modelling, bootstrap re-sampling algorithms should be used to better analyze available data and thereby take full advantage of them. This issue is of importance in the animal sciences as producing reliable data is both expensive and time-consuming.

Abstract 048: Circulating Branched-Chain Amino Acids and Incident Cardiovascular Disease in a Prospective Cohort of US Women

Circulation ◽  
2018 ◽  
Vol 137 (suppl_1) ◽  
Author(s):  
Deirdre K Tobias ◽  
Patrick R Lawler ◽  
Paulo H Harada ◽  
Olga V Demler ◽  
Paul M Ridker ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Cardiovascular Disease ◽  
Amino Acids ◽  
Prospective Cohort ◽  
Branched Chain Amino Acids ◽  
Health Study ◽  
Blood Collection ◽  
Cvd Risk ◽  
Isoleucine Leucine ◽  
Branched Chain

Introduction: Recent metabolomics studies have identified circulating levels of branched-chain amino acids (BCAAs; isoleucine, leucine, valine) as strong predictors of type 2 diabetes (T2D). Whether BCAAs are implicated in cardiovascular disease (CVD) risk has not been established. Hypothesis: We hypothesized that higher baseline levels of plasma BCAAs are associated with an elevated risk of incident CVD events, and evaluated whether this relationship was dependent on an intermediate diagnosis of T2D. Methods: Participants enrolled in the Women’s Health Study prospective cohort were eligible if they did not report CVD or cancer prior to baseline blood collection (N=27,172, mean baseline age=54.7 years). Plasma BCAA metabolites were measured via proton NMR spectroscopy, ln-transformed, and standardized for analysis. We used multivariable Cox proportional regression models to estimate hazard ratios (HR) and 95% confidence intervals (CI) per standard deviation (SD) of total and individual BCAAs with incident CVD (myocardial infarction [MI], stroke, coronary revascularization). Results: 1,917 confirmed CVD events occurred over follow-up (mean 18.6 years). In models adjusted for age, body mass index, smoking status, diet quality, physical activity, and other established CVD risk factors, total BCAAs were positively associated with CVD (per SD, HR=1.13, CI=1.08 to 1.19), comparable in magnitude to the association of LDL cholesterol with CVD (per SD, HR=1.15, CI=1.09 to 1.21). In particular, BCAAs were associated with coronary events (MI: HR=1.21, CI=1.10 to 1.33; revascularization: HR=1.15, CI=1.07 to 1.23), but not with stroke (HR=1.07, CI=0.98 to 1.15). The BCAA-CVD relationship was notably greater (p-interaction=0.008) among participants who developed T2D prior to a CVD event (HR=1.25, CI=1.13 to 1.39), vs. women without T2D (HR=1.07, CI=1.01 to 1.13). Isoleucine, leucine, and valine were each associated with CVD (p<0.05). Further adjusting for biomarkers of potential intermediates, HbA1c, lipids, and a lipoprotein-based insulin resistance score entirely eliminated the associations of BCAAs with CVD. Conclusions: Circulating plasma BCAAs were positively associated with long-term incident CVD in a cohort of US women, in particular among women who developed T2D prior to a CVD event. Impaired BCAA metabolism may represent a shared pathway of insulin resistance that links the risks of T2D and CVD.

scholarly journals Association of Dietary Branched-Chain Amino Acids With Obesity: The Filipino Women's Diet and Health Study (FiLWHEL)

2021 ◽  
Vol 5 (Supplement_2) ◽  
pp. 1072-1072
Author(s):  
Akinkunmi Okekunle ◽  
Heejin Lee ◽  
Sherlyn Mae Provido ◽  
Grace Chung ◽  
Sangmo Hong ◽  
...  
Keyword(s):  
Amino Acids ◽  
Statistical Significance ◽  
Branched Chain Amino Acids ◽  
Health Study ◽  
Dietary Intakes ◽  
Obesity Risk ◽  
Isoleucine Leucine ◽  
Dietary Recall ◽  
Filipino Migrants ◽  
Branched Chain

Abstract Objectives Migration plays a significant role in dietary choices and health of populations. Studies on dietary intakes of branched-chain amino acids (BCAA) and health status of migrant populations are scarce. This study examined the association between dietary BCAA intake and risk of obesity among migrant Filipino women in Korea. Methods This study included 428 women (20–57years) enrolled in the FiLWHEL study. Demographic information and anthropometric measurements (weight and height) were obtained using a standard protocol. Dietary BCAA (isoleucine, leucine, and valine) intakes were derived from a one-day 24-hour dietary recall. Body mass index (BMI) was calculated from weight and height. Obesity was defined as BMI ≥ 25 kg/m2. Energy-adjusted BCAA intakes were categorized in quartile distribution with the lowest quartile as a reference and multivariable-adjusted odds ratio (OR) with 95% confidence interval (CI) of obesity risk were estimated using logistic regression at a statistical significance of P &lt; 0.05. Results Mean age and BMI were 35.0 ± 8.1 years and 23.6 ± 3.9 kg/m2 respectively. 30.8% had BMI ≥ 25 kg/m2. Also, median and interquartile range of BCAA intakes (mg/day) were isoleucine: 1920.9 (1231.9–2719.1), leucine: 3318.9 (2134.2–4774.1), valine: 2257.3 (1442.6–3283.1) and total BCAA: 7519.0 (4762.0–10,726.9). Multivariable-adjusted OR and 95% CI for obesity risk given dietary BCAA intakes for each subsequent quartile compared to the bottom quartile were; isoleucine: 0.48 (0.27–0.89), 0.67 (0.37–1.02), and 0.49 (0.27–0.89) P for trend = 0.09; leucine: 0.69 (0.37–1.28), 0.80 (0.44–1.46), and 0.62 (0.34–1.13) P for trend = 0.23; valine: 0.51 (0.27–0.95), 0.77 (0.43–1.37), and 0.52 (0.28–0.95) P for trend = 0.15 and total BCAA: 0.58 (0.31–1.09), 0.82 (0.45–1.48), and 0.56 (0.31–1.03) P for trend = 0.23. Conclusions Dietary BCAA intake appears inversely related to the odds of obesity in this sample of Filipino migrants in Korea. Cohort studies among migrant population might significantly benefit the validation of these observations. Funding Sources This work was supported by the Hanmi Pharmaceutical Co., Ltd, (No. 201300000001270), Chong Kun Dang Pharm. Seoul, Korea (No. 201600000000225) and the Brain Pool Program through the National Research Foundation of Korea funded by the Ministry of Science and ICT (No. 2020H1D3A1A04081265).

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