scholarly journals Metagenome-Assembled Genome (MAG) of Oceancaulis alexandrii NP7 isolated from Mediterranean Sea polluted marine sediments and its bioremediation potential

Author(s):  
Filippo Dell’Anno ◽  
Leonardo Joaquim van Zyl ◽  
Marla Trindade ◽  
Christophe Brunet ◽  
Antonio Dell’Anno ◽  
...  

Abstract Oceanicaulis alexandrii strain NP7 is a marine bacterium which belongs to the Hyphomonadaceae family and was isolated from sediments highly contaminated with metals and polycyclic aromatic hydrocarbons released for decades by industrial activities in the Gulf of Naples (Mediterranean Sea). Here, we report the partial genome sequence and annotation of Oceanicaulis alexandrii strain NP7 that contains a chromosome of 2,954,327 bp and encodes for 2914 predicted coding sequences and 44 RNA- encoding genes. Although the presence of some coding sequences for genes involved in hydrocarbon degradation processes (e.g., alkB) have already been described in the literature associated with the Oceanicaulis, this is the first time that more than 100 genes involved in metal detoxification processes and hydrocarbon degradation are reported belonging to this genus. The presence of a heterogeneous set of genes involved in stress response, hydrocarbon degradation, heavy metal resistance and detoxification suggests a possible role for Oceanicaulis alexandrii NP7 in the bioremediation of these highly contaminated marine sediments.

2008 ◽  
Vol 25 (6) ◽  
pp. 304-314 ◽  
Author(s):  
A.-C. M. Toes ◽  
J. S. Geelhoed ◽  
J. G. Kuenen ◽  
G. Muyzer

2016 ◽  
Vol 60 (9) ◽  
pp. 5589-5591 ◽  
Author(s):  
Katrin Zurfluh ◽  
Jochen Klumpp ◽  
Magdalena Nüesch-Inderbinen ◽  
Roger Stephan

ABSTRACTHere, we present the full sequences of threemcr-1-carrying plasmids isolated from extended-spectrum-β-lactamase (ESBL)-producingEscherichia coli. The plasmids belong to three different replicon types and are 34,640 bp, 209,401 bp, and 247,885 bp in size. We describe for the first time a composite transposon containingmcr-1localized on a multidrug-resistant (MDR) IncHI2 plasmid harboring additional determinants of resistance to six different classes of antibiotics, including the ESBL geneblaCTX-M-1, and heavy metal resistance.


1994 ◽  
Vol 84 (1-2) ◽  
pp. 45-56 ◽  
Author(s):  
Marina Montresor ◽  
Elvira Montesarchio ◽  
Donato Marino ◽  
Adriana Zingone

Author(s):  
Yan Guo ◽  
Chang-ye Hui ◽  
Nai-xing Zhang ◽  
Lisa Liu ◽  
Hui Li ◽  
...  

The development of genetic engineering, especially synthetic biology, greatly contributes to the development of novel metal biosensors. The cad operon encoding cadmium resistance was previously characterized from Pseudomonas putida. In this study, single-, dual-, and triple-signal output Cd(II) biosensors were successfully developed using artificial translationally coupled cad operons. Sensitivity, selectivity, and response toward Cd(II) and Hg(II), of three biosensors were all determined. Reporter signals of three biosensors all increased within the range 0.1–3.125 μM Cd(II). Three biosensors responded strongly to Cd(II), and weakly to Hg(II). However, the detection ranges of Cd(II) and Hg(II) do not overlap in all three biosensors. Next, novel Cd(II) biosensing coupled with bioadsorptive artificial cad operons were assembled for the first time. Cd(II)-induced fluorescence emission, enzymatic indication, and Cd(II) binding protein surface display can be achieved simultaneously. This study provides an example of one way to realize multiple signal outputs and bioadsorption based on the redesigned heavy metal resistance operons, which may be a potential strategy for biodetection and removal of toxic metal in the environment, facilitating the study of the mechanism and dynamics of bioremediation.


2020 ◽  
Vol 9 (49) ◽  
Author(s):  
Natsuko Hamamura ◽  
Nobuyoshi Nakajima ◽  
Shigeki Yamamura

ABSTRACT The antimony-oxidizing Stenotrophomonas sp. strain SbOxS2 was isolated from stibnite mine tailing soil. The draft genome sequence of strain SbOxS2 comprises 4.76 Mbp with 4,211 predicted protein-coding sequences. This genome will provide useful information for characterizing the molecular mechanisms associated with heavy metal resistance within the genus Stenotrophomonas.


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