P–222 Can we optimise the time that we perform the fertilisation check in the lab? Lessons learnt from time-lapse incubation

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Barrie ◽  
R Smith ◽  
L Best ◽  
N Davis ◽  
S Duffy ◽  
...  
Keyword(s):  
Study Design ◽  
Fetal Heart ◽  
Time Lapse ◽  
Trial Registration ◽  
Optimum Time ◽  
Use Of Time ◽  
Lessons Learnt ◽  
Registration Number

Abstract Study question Can time-lapse data be used to identify the optimum time to perform the fertilisation check for oocytes cultured in standard incubation? Summary answer The optimum time to perform fertilisation checks for oocytes cultured in standard incubation is 16.5hpi+/–0.5h. What is known already Time-lapse incubation allows the embryologist to retrospectively review collated images of oocytes and embryos to capture important embryological observations that may have otherwise been missed. This is a luxury not available to embryologists when oocytes or embryos are cultured in standard incubation. Traditionally, the optimum time to perform the fertilisation check is 17 hours post insemination (hpi) +/- 1 hour. It was hypothesised that this could be fine-tuned ensuring the maximum number of fertilised oocytes were observed, thereby increasing the number of usable embryos for the patient. Study design, size, duration This was a retrospective, multicentre analysis including data from 27,022 ICSI derived embryos cultured in time-lapse incubation between January 2011 to November 2019. Participants/materials, setting, methods The time of pronuclei appearance and disappearance was recorded using the time-lapse incubation software. The number of oocytes exhibiting normal fertilisation (defined as the presence of two pronuclei) during 30 minute intervals from 15hpi to 20hpi was determined. Main results and the role of chance Between 15–17.5hpi the average number of oocytes exhibiting normal fertilisation was 98.19% with most oocytes having visible pronuclei at 16–16.5hpi (98.32%). At 18–18.5hpi the number of visible pronuclei falls to 95.53% and continues to fall to 87.02% at 19.5–20hpi meaning that over 3000 (11%) normally fertilised oocytes, within this cohort, would not be identified. Limitations, reasons for caution The conclusions of this investigation cannot be effectively extrapolated to IVF embryos as only ICSI embryos were used for the determination of the results. Wider implications of the findings: The optimum time to perform fertilisation checks for oocytes cultured in standard incubation is 16.5hpi+/–0.5h. However, without the use of time-lapse incubation, the fertilisation of at least 2% of embryos that create a fetal heart will be missed, even if the fertilisation check is performed in the optimum window (16.5hpi+/–0.5h). Trial registration number Not applicable

scholarly journals The determination of glacier speed by time-lapse photography under unfavorable conditions

1992 ◽  
Vol 38 (129) ◽  
pp. 257-265 ◽  
Author(s):  
W.D. Harrison ◽  
K.A. Echelmeyer ◽  
D.M. Cosgrove ◽  
C. F. Raymond
Keyword(s):  
Time Lapse ◽  
Systematic Errors ◽  
Horizontal Motion ◽  
Limited Information ◽  
Glacier Surface ◽  
Alaska Range ◽  
Use Of Time ◽  
West Fork ◽  
Time Lapse Photography

AbstractTwo practical problems in the use of time-lapse photography for the measurement of speed were encountered during the recent surge of West Fork Glacier in the central Alaska Range, Alaska, U.S.A. The first is severe rotational camera instability; we show how natural, unsurveyed features on the valley wall can be used to make the necessary corrections. The second problem is the computation of absolute speed when many different, unsurveyed glacier-surface features are used as targets. We give a method for connecting the data obtained from different targets, and for determining the scale using limited information obtained by surveying. Severe systematic errors can occur unless the angle between the axis of the lens and the direction of horizontal motion is determined.

The Role of Improved K-Means Clustering Algorithm in the Motion Parameters Determination of Breaker's Moving Contact

2014 ◽  
Vol 960-961 ◽  
pp. 905-909
Author(s):  
Yu Tao Xu ◽  
Shu Tao Zhao ◽  
Dan Jiang ◽  
Jian Feng Ren
Keyword(s):  
Clustering Algorithm ◽  
Circuit Breaker ◽  
A Algorithm ◽  
Use Of Time ◽  
Moving Contact ◽  
Center Point ◽  
Movement Distance ◽  
Motion Parameters

High voltage circuit breaker's motion parameters of the moving contact is one of the key performance indicators in the evaluated of breaker. By means of extracting color features of LW10B-252 circuit breaker by image segmentation, then determining its center point of the minimum circumscribed rectangle, getting the movement distance of center point and the use of time in adjacent images, realization motion parameter's measurement of the moving contact. This paper presents a algorithm based on improved k-means clustering algorithm to extract the color characteristics of the mechanical linkage of breaker, at the same time contrast with classical OTSU method and RGB color segmentation method. Experimental results show that the k-means clustering algorithm have a better segmentation result.

scholarly journals Systemic Inflammation and Clinical Outcomes in COVID-19: a retrospective study

2020 ◽  
Author(s):  
Meijia Wang ◽  
Zhenli Huang ◽  
Kun Tang ◽  
Pengfei Gao ◽  
Yanjiao Lu ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Clinical Outcomes ◽  
Systemic Inflammation ◽  
Trial Registration ◽  
Inflammatory Factors ◽  
Reactive Protein ◽  
Tnf Α ◽  
Registration Number ◽  
Factor Α

Abstract Background：COVID-19 causes epidemics and pandemics worldwide, but the role of pathophysiological parameters particularly systemic inflammation in COVID-19 has not been understood. We aimed to investigate clinical outcomes in view of systemic inflammation in COVID-19.Methods：In this retrospective study, the demographic and clinical data of 225 confirmed COVID-19 cases on admission at Tongji Hospital from January 28 to February 15, 2020, were extracted and analyzed. These patients were categorized by inflammation state on the basis of the expression of inflammatory factors or classified as severe and non-severe according to 2019 American Thoracic Society / Infectious Disease Society of America guidelines.Results: Among 225 patients with confirmed COVID-19, 155 patients (68.9%) categorized into hyperinflammation group and 70 (31.1%) were non- hyperinflammation group. Compared to non-hyperinflammation group, hyperinflammation group more frequently had chest tightness/dyspnea and lymphopenia, aberrant multiple indexes of organ function including the heart, liver, kidney, and coagulation, with higher level of C-reactive protein (hsCRP) as well as interleukin (IL)-6, IL-8, tumour necrosis factor α (TNF-α), etc. Hyperinflammation group were more likely to admit to intensive care unit (ICU) (52.3% vs 5.7%), receive ventilation (84.5% vs 10.0%) and be with higher mortality (44.5% vs 5.7%) than non-hyperinflammation group. The mortality of severe patients with hyperinflammation (60/99, 60.6%) was significantly higher than without hyperinflammation (2/20, 10.0%). Non-severe patients with hyperinflammation even tended to have higher mortality (9/56, 16.1%) than those in severe cases without hyperinflammation (2/20, 10%).Conclusion: Excessive systemic inflammation was correlated highly with poor clinical outcomes in COVID-19, particularly in severe cases. Non-severe patients with hyperinflammation even tended to have higher mortality than those in severe cases without hyperinflammation.Trial registration: This is a retrospective observational study without a trial registration number.

P–150 Does trophectoderm biopsy performed on different blastocyst stages affect the clinical outcome?

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S H Tan ◽  
A Q Y Chan ◽  
A Y X Lim ◽  
M W Lim
Keyword(s):  
Retrospective Study ◽  
Clinical Outcome ◽  
Sample Size ◽  
Time Lapse ◽  
Blastocyst Stage ◽  
Assisted Hatching ◽  
Preimplantation Genetic Testing ◽  
Significant Difference ◽  
Registration Number

Abstract Study question The objective of this study is to evaluate the effect of trophectoderm (TE) biopsy on different blastocyst stages and its clinical outcome. Summary answer Our results showed that TE biopsy significantly reduced the clinical outcome of fully hatched blastocyst. What is known already: TE biopsy is a method widely practiced to harvest cells to determine the chromosomal constitution of a blastocyst, ensuring higher implantation and healthy pregnancies. The effect on clinical outcome after transferring blastocysts biopsied at different blastocysts stages has not been extensively studied. Study design, size, duration This retrospective study was conducted from January 2017 until July 2019 at Alpha IVF & Women’s Specialists. Following laser assisted hatching on day 3, TE biopsy was performed on unhatched, hatching and fully hatched day–5 blastocysts. A total of 1,020 single euploid blastocysts transfer (SBT) were performed. The average maternal age was 31.7. Implantation rates (IR) were evaluated for all stages of hatching (Unhatched: BG3 & 4; hatching: BG5; fully hatched: BG6). Participants/materials, setting, methods Laser assisted hatching (Hamilton Thorne Bioscience, USA) was performed on day–3 and subsequently cultured to blastocyst-stage. Different hatching stages were observed using embryoscope time-lapse system (Vitrolife, Sweden) and were recorded. Day–5 blastocysts with at least BG3BB grade (Gardner’s System) were selected for TE biopsy and the biopsied cells were sent for preimplantation genetic testing for aneuploidy (PGT-A) using Next-Generation Sequencing (Life Technologies, USA). All blastocysts were vitrified and warmed using the Cryotec Method (Cryotech, Japan). Main results and the role of chance: All 1,020 blastocysts survived post-warmed (post-warm survival rate= 100%) and were transferred in frozen transfer cycles. TE biopsy performed on unhatched blastocysts showed a comparable IR to hatching blastocysts (60.0% [15/25] and 65.2% [627/961]). While fully hatched blastocysts (44.12% [15/34]) show a significantly lower IR when compared to hatching blastocysts (65.2% [627/961]), no significant difference was seen when comparing unhatched blastocysts to fully hatched blastocysts (60.0% [15/25] and 44.12% [15/34]; p = 0.2949). Limitations, reasons for caution The sample size was comparatively smaller in the unhatched and fully hatched group than the hatching group. Further studies with a larger sample size is recommended to ascertain the clinical outcome. Since this is a retrospective study and biopsy was done by different embryologists, the biopsy technique was not controlled. Wider implications of the findings: To achieve higher clinical pregnancy, it is recommended to perform TE biopsy before the blastocysts is fully hatched. Trial registration number Not applicable

P–156 Automatic pronuclear detection based on deep learning technology has clinical utility

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
S Takeda ◽  
N Fukunaga ◽  
S Sanami ◽  
Y Tsuzuki ◽  
H Kitasaka ◽  
...  
Keyword(s):  
Deep Learning ◽  
Evaluation System ◽  
Clinical Utility ◽  
Detection System ◽  
Time Lapse ◽  
Learning Technology ◽  
Training Images ◽  
First Results ◽  
Registration Number

Abstract Study question Does the performance of an automatic pronuclear detection system based on deep learning technology have clinical utility? Summary answer Output results for 2PN detection using the automatic system powered by deep learning technology has clinical utility. What is known already In order to establish a more objective embryo evaluation system, we have been developing an automatic pronuclear detection system that utilizes deep learning technology based on Time- Lapse (TL) images. We have previously reported that the accuracy of pronuclei detection was improved by introducing an analysis method using 11 slices in the Z axis. In this study, we evaluated the potential clinical practicality of the automatic pronuclear detection system. Study design, size, duration Embryos clinically evaluated between May 2018 and December 2019 by embryologists were chosen for this study. We prepared for analysis TL videos of 995 embryos that had been evaluated as having 0, 1, 2, and 3PN. Participants/materials, setting, methods Part1:We compared the outputs of the automatic pronuclear detection system with these embryologists(three junior embryologists (1a), three intermediate embryologists (1b),and three senior embryologists (1c)) who had judged the pronuclei number from TL videos from 40 embryos each having 0,1,2,and 3PN. Part2:The automatic pronuclear detection system determined the pronuclei number from the TL videos of 955 embryos scored as either 1,2,and 3PN,(different from those used in Part1),and the detection rate for 2PN was calculated. Main results and the role of chance Part1: The sensitivities for embryologist groups 1a),1b),1c) and the automatic pronuclear detection system were 80.0%,100%,100%,100% for 2PN, 60.0%,83.3%,86.7%,100% for 0PN, 46.7%,80.0%,86.7%,10.0% for 1PN, and 73.3%,96.7%,96.7%,10.0% for 3PN. Part2: The precision for 2PN by the automatic pronuclear detection system was 99%. Limitations, reasons for caution In order to further improve the performance of the automatic pronuclear detection system, further adjustment of the algorithm and more training images will be utilised. Wider implications of the findings: The detection of 2PN by the automatic pronuclear detection system was highly reliable, and the performance of the system was comparable to that of embryologists. These first results are reassuring and support the clinical use of the system as a further aid for embryologists, in routine laboratory practice. Trial registration number ‘not applicable’

P–317 Uterine cavity in patients with repeated implantation failure (RIF) and before the first IVFprogram

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Rybina ◽  
V Lokshin ◽  
Y Askar ◽  
R Valiev ◽  
S Karibayeva
Keyword(s):  
Antibiotic Therapy ◽  
Study Design ◽  
Uterine Cavity ◽  
Visual Examination ◽  
Chronic Endometritis ◽  
Registration Number ◽  
Group 2 ◽  
First Time ◽  
Group 1

Abstract Study question What is the frequency of uterine pathology and histological analysis of chronic endometritis in the RIF group and in patients before the first IVF program? Summary answer We report a lower incidence of chronic endometritis in the group of patients just preparing for the first IVF program and higher effectiveness What is known already Chronic endometritis (CE) is one of the reasons for implantation failures in IVF cycles. The presence of chronic inflammation in the endometrium changes its susceptibility, resulting in failed implantations and loss of pregnancy. CE diagnostics is rather complicated. Visual examination during hysteroscopy allows one to suspect CE in 20–70%, routine histology - in 10–20%. The new gold standard is immunohistochemistry (IHC). Study design, size, duration A continuous study of 2003 hysteroscopies was carried out, conducted in 1944 patients from May 2018 to January 2020. All patients were divided into 2 groups: 1 - 650 patients preparing for IVF for the first time; 2 - 1294 with repeated implantation failures. Participants/materials, setting, methods All included patients had diagnostic office hysteroscopy (OH) with biopsy and IHC CD 138, CD 20, and CD 8. We compared the frequency of CE detection by visual examination during hysteroscopy, routine histology and IHC of the two groups, as well as the effectiveness of 1 course of antibiotic therapy with CE. Main results and the role of chance In group 1 of women preparing for IVF for the first time, during visual examination by hysteroscopy, CE was detected in 17% (110/650), in group 2 RIF - in 27% (349/1294), p < 0.001. Routine histology also more often detected CE in the RIF group, 42% (543/1294) compared with group 1 - 23% (149/650), p < 0.001. IHC markers of CE were detected in 81.8% (1058/1294) of samples from RIP group than in group 1 - 71.4% (464/650), p < 0.001. At the same time, IHC mild CE was more common in group 1 in 320/650 (49.2%), p < 0.001. In the second group, mild, moderate and severe CE occurred with the same frequency (33.2%, 36.5%, 30.3%, respectively). The frequency of CE detection by IHC in the groups after 1 course of antibiotic therapy with fluoroquinolones was 31.2% (145/464) and 43.2% (457/1058), respectively, p < 0.001. The pregnancy rate (PR) also differed and was the lowest in the RIF group: in group 1 after treatment with CE PR = 42.7% (198/464), in group 2 after treatment with CE PR = 27.2% (288 / 1058), p < 0.05. Limitations, reasons for caution The limitations are related to study design. More research is also needed. Wider implications of the findings: The question of overdiagnosis of CE using IHC remains open. Therefore, well-designed prospective studies or RCTs should be conducted to clarify possible correlations between ChE and poor reproductive outcomes and the effectiveness of endometrial interventions and treatments. Trial registration number not avaliable

Evidence for a functional role of the cytoskeleton in determination of the dorsoventral axis in Xenopus laevis eggs

Development ◽  
1983 ◽  
Vol 77 (1) ◽  
pp. 15-37
Author(s):  
Geertje A. Ubbels ◽  
K. Hara ◽  
C. H. Koster ◽  
M. W. Kirschner
Keyword(s):  
Functional Role ◽  
Normal Development ◽  
Cytological Study ◽  
Time Lapse ◽  
Microtubule Assembly ◽  
Dorsoventral Axis ◽  
Normal Table ◽  
Fertilized Egg

A normal table of events of the first cleavage period in the fertilized egg (cf. Gerhart, 1980) has been completed (cf. Table I) by studying external and internal features. Through a cytological study of eggs fixed after video time-lapse observation such featurescan directly be correlated and it has been shown that the first postfertilization wave (PFW) reflects spermaster growth, which causes rearrangements of animal yolk material. Thismay, in conjunction with the interaction of the spermaster rays with the cortex, define, in time as well as in space, the asymmetric cortical contraction which we suppose to evoke asymmetry in the animal hemisphere by formation of the vitelline wall (Pasteels, 1964) and in the vegetal hemisphere by formation of the Vegetal Dorsalising Centre (Kirschneret al. 1981). Neither prick-activated eggs nor fertilized eggs incubated in vinblastine develop a spermaster. Under these conditions abnormal cytoplasmic segregation may be directed by gravity alone. For normal development the activated egg must in some way, for instance through the sperm centriole, organize microtubule assembly into a monaster. The centriole actsas a microtubule-organizing centre in structuring the egg's cytoskeleton, and through this directs localization of the various yolk components, in time as well as in space. In egg rotation experiments performed under appropriate conditions, the cytoskeleton is disturbed and yolk rearranges under gravity till a new equilibrium is established which determines a new dorsoventral polarity. Such experiments also show that neither the dorsal cytoplasm nor the grey crescent cortex act as the ultimate dorsal determinants, since their localization is unaltered upon rotation, whereas the overall yolk distribution is significantly changed.

scholarly journals The determination of glacier speed by time-lapse photography under unfavorable conditions

1992 ◽  
Vol 38 (129) ◽  
pp. 257-265 ◽  
Author(s):  
W.D. Harrison ◽  
K.A. Echelmeyer ◽  
D.M. Cosgrove ◽  
C. F. Raymond
Keyword(s):  
Time Lapse ◽  
Systematic Errors ◽  
Horizontal Motion ◽  
Limited Information ◽  
Glacier Surface ◽  
Alaska Range ◽  
Use Of Time ◽  
West Fork ◽  
Time Lapse Photography

AbstractTwo practical problems in the use of time-lapse photography for the measurement of speed were encountered during the recent surge of West Fork Glacier in the central Alaska Range, Alaska, U.S.A. The first is severe rotational camera instability; we show how natural, unsurveyed features on the valley wall can be used to make the necessary corrections. The second problem is the computation of absolute speed when many different, unsurveyed glacier-surface features are used as targets. We give a method for connecting the data obtained from different targets, and for determining the scale using limited information obtained by surveying. Severe systematic errors can occur unless the angle between the axis of the lens and the direction of horizontal motion is determined.

P–282 A time lapse analysis of 36,671 embryos to compare the incidence of early stage abnormal cleavage events in ICSI and IVF derived embryos

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A Campbell ◽  
S Montgomery ◽  
B Richardson ◽  
C Duncan ◽  
C Howles
Keyword(s):  
Pregnancy Outcomes ◽  
Maternal Age ◽  
Early Stage ◽  
Time Lapse ◽  
Advanced Maternal Age ◽  
Early Embryo ◽  
Male Factor ◽  
Registration Number ◽  
Time Lapse Imaging

Abstract Study question Is this incidence of early stage abnormal cleavage events different between embryos created following ICSI compared with IVF? Summary answer Embryos derived from ICSI are more likely to exhibit abnormal cleavage compared with those from IVF. This difference is most marked in women ≥35 years. What is known already Time lapse imaging (TLI) has been instrumental in allowing detailed annotation of early embryo development to provide an objective aid for embryo selection in ART cycles. Amongst several abnormal cleavage events reported, rapid cleavage and multichotomous mitosis/direct cleavage, during the first days after fertilisation have been demonstrated to be associated with lower blastulation rates, reduced implantation potential, increased aneuploidy and poor pregnancy outcomes. With ICSI being utilised commonly, and being the insemination method of choice in some clinics, the incidence of abnormal cleavage was investigated in association with insemination method, ICSI or IVF. Study design, size, duration The incidence of abnormal cleavage events was evaluated in a large multicentre retrospective analysis of 36,671 embryos from 6689 patients treated in 8 IVF clinics enabled with time lapse imaging, between 2011 - 2019. This constituted 10931 IVF embryos and 25740 ICSI embryos. Participants/materials, setting, methods Following ICSI or after IVF fertilisation check, embryos were time-lapse imaged every 10 minutes and annotated using the EmbryoScope. Second cell cycle durations were calculated as follows: time to reach 3-cell (t3) from 2-cell (t2) (t3-t2 = cc2). These were analysed using a welch t-test as three groups of abnormal cleavage: direct cleavage/trichotomous mitosis (DC) - where cc2=0 hours(h), rapid cleavage within 2h (R2) - where 0<cc2<2h and rapid cleavage between 2–5h (R5) where 2h<cc2<5h. Main results and the role of chance The incidence of DC, R2 and R5 in the whole cohort of embryos was 5%, 8% and 9% respectively. In the subpopulation of IVF embryos the incidence of DC, R2 and R5 was 4%, 8% and 9% respectively. In the subpopulation of ICSI embryos the incidence of DC, R2 and R5 was 6%, 8% and 9% respectively. The incidence of DC was significantly higher in ICSI embryos compared with IVF (p < 0.001) whilst R2 and R5 were the same. ICSI derived embryos had a mean (± SE) cc2 value of 9.39 ± 0.03h, compared with 9.56 ± 0.05h for IVF embryos (p < 0.0038). Examination of data split by maternal age demonstrated that ICSI oocytes from women of advanced maternal age (≥35) also had significantly more embryos exhibiting rapid cleavages R2 and R5 than IVF oocytes (p < 0.007). There were no significant differences however, in rates of abnormal cleavages between ICSI and IVF in embryos from women aged <30 (p = 0.06). Male-factor diagnoses showed no significant differences in abnormal cleavage values between ICSI or IVF in all three abnormal cleavage categories. Limitations, reasons for caution This analysis could not control for all potential confounders therefore it is possible that the increased abnormal cleavages observed in this investigation are a result of another, or combination of factors. Despite quality assurance programs being in place across all clinics, there is a risk of annotation bias. Wider implications of the findings: There is a higher incidence of early abnormal cleavage in embryos derived from ICSI, particularly in those from women of increased age and this research may help elucidate the reasons for this and add to the debate regarding the appropriateness of the increasing use of ICSI. Trial registration number Not applicable

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